##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743470
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.146083903856244	34.0	33.0	34.0	31.0	34.0
2	33.28002609385718	34.0	34.0	34.0	31.0	34.0
3	33.39707452889827	34.0	34.0	34.0	31.0	34.0
4	36.61272680807564	37.0	37.0	37.0	35.0	37.0
5	36.64624934429096	37.0	37.0	37.0	35.0	37.0
6	36.68563223801902	37.0	37.0	37.0	36.0	37.0
7	36.660552544151074	37.0	37.0	37.0	35.0	37.0
8	36.64899592451612	37.0	37.0	37.0	35.0	37.0
9	38.49040714487471	39.0	39.0	39.0	37.0	39.0
10-11	38.51347263507606	39.0	39.0	39.0	37.5	39.0
12-13	38.50624773023794	39.0	39.0	39.0	37.0	39.0
14-15	40.11358494626548	41.0	40.0	41.0	38.0	41.0
16-17	40.1148318022247	41.0	40.0	41.0	38.0	41.0
18-19	40.099139844243886	41.0	40.0	41.0	38.0	41.0
20-21	40.09587609452971	41.0	40.0	41.0	38.0	41.0
22-23	40.01813186813187	41.0	40.0	41.0	38.0	41.0
24-25	39.98216404158877	41.0	40.0	41.0	38.0	41.0
26-27	39.920152797019384	41.0	40.0	41.0	38.0	41.0
28-29	39.88693155070144	41.0	40.0	41.0	38.0	41.0
30-31	39.82768033679906	41.0	40.0	41.0	38.0	41.0
32-33	39.78292869920777	41.0	40.0	41.0	37.5	41.0
34-35	39.699235342380994	41.0	40.0	41.0	37.0	41.0
36-37	39.58884084092162	41.0	40.0	41.0	37.0	41.0
38-39	39.4817390076264	41.0	40.0	41.0	36.5	41.0
40-41	39.3426500060527	41.0	39.0	41.0	36.0	41.0
42-43	39.240132083338935	41.0	39.0	41.0	35.0	41.0
44-45	39.003396909088465	41.0	39.0	41.0	35.0	41.0
46-47	38.77541393734784	40.0	38.0	41.0	35.0	41.0
48-49	38.67305540236997	40.0	38.0	41.0	35.0	41.0
50-51	38.50912007209437	40.0	37.5	41.0	35.0	41.0
52-53	38.25700633515811	40.0	37.0	41.0	35.0	41.0
54-55	38.04957093090508	40.0	36.5	41.0	34.5	41.0
56-57	37.80903936944328	39.5	36.0	41.0	34.0	41.0
58-59	37.5277032025502	39.0	35.0	41.0	34.0	41.0
60-61	37.248355683484206	39.0	35.0	41.0	34.0	41.0
62-63	36.9894757017768	38.0	35.0	41.0	33.0	41.0
64-65	36.70368205845562	37.0	35.0	40.0	33.0	41.0
66-67	36.39138095686444	37.0	35.0	40.0	33.0	41.0
68-69	36.100836617482884	36.0	35.0	39.5	33.0	41.0
70-71	35.78602566344304	36.0	35.0	39.0	32.5	41.0
72-73	35.462114140449515	35.0	35.0	39.0	32.0	40.5
74-75	35.14107495931242	35.0	35.0	37.5	32.0	40.0
76-77	33.323456225537015	34.5	32.0	35.5	28.5	38.5
78-79	34.62981626696437	35.0	34.5	36.5	32.0	39.0
80-81	34.572297469971886	35.0	35.0	36.0	32.0	38.0
82-83	34.386671957173796	35.0	35.0	36.0	32.0	37.0
84-85	34.20140557117301	35.0	35.0	35.5	32.0	37.0
86-87	34.02258934455997	35.0	35.0	35.0	32.0	36.5
88-89	33.883121713048276	35.0	34.0	35.0	31.5	36.0
90-91	33.75475002353828	35.0	34.0	35.0	31.0	36.0
92-93	33.67668433158029	35.0	34.0	35.0	31.0	36.0
94-95	33.5858978842455	35.0	34.0	35.0	31.0	35.5
96-97	33.53521056666712	35.0	34.0	35.0	31.0	35.0
98-99	33.499912572128	35.0	34.0	35.0	31.0	35.0
100-101	32.62523370142709	34.5	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	16.0
15	43.0
16	107.0
17	203.0
18	388.0
19	563.0
20	836.0
21	1222.0
22	1733.0
23	1758.0
24	1684.0
25	2057.0
26	2635.0
27	3455.0
28	4388.0
29	4961.0
30	5632.0
31	6475.0
32	8178.0
33	11298.0
34	19123.0
35	38006.0
36	101741.0
37	219685.0
38	244548.0
39	62715.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.638063907487954	18.050716195285194	12.673642553812636	24.637577343414222
2	16.3347545966885	19.290354688151506	38.5663174035267	25.80857331163329
3	20.025421335090858	25.043377674956623	27.929842495325975	27.001358494626547
4	12.96905053331002	16.248268255612196	37.93952681345582	32.84315439762196
5	14.178379759775108	37.38819320214669	34.48316677202846	13.95026026604974
6	31.875798619984668	35.60062948067844	18.540627059598908	13.982944839737984
7	28.33066566236701	30.318775471774245	21.84163449769325	19.508924368165495
8	26.811841769002108	33.84023565174116	19.883115660349443	19.464806918907286
9	26.976878690464982	15.118969158136844	19.461040795190122	38.44311135620806
10-11	26.66038979380473	24.331311283575666	27.484632870189785	21.52366605242982
12-13	27.109029281611903	22.26041400460005	27.292426056195946	23.338130657592103
14-15	23.985634928107388	24.137221407723246	24.54308849045691	27.334055173712457
16-17	24.056855017687333	26.67599230634726	25.562161216995978	23.704991458969427
18-19	23.813536524674834	26.13232544689093	26.55930972332441	23.49482830510982
20-21	24.913781740021683	24.97390586203464	26.229518119285185	23.882794278658494
22-23	24.8641152663018	25.408999261561803	25.609077006056808	24.117808466079595
24-25	24.475582322024835	25.139834626261404	26.021581306513063	24.363001745200698
26-27	24.378203808993465	26.09788674707546	25.88361573257686	23.640293711354214
28-29	24.106669069910865	26.00904310303663	26.26151825400722	23.622769573045286
30-31	24.269902160433215	25.261748414848373	26.265565691319797	24.202783733398615
32-33	24.0680836296922	25.686915912991548	26.383112655823787	23.861887801492465
34-35	24.780582879478967	25.31417841487019	25.870169628769773	24.03506907688107
36-37	23.99854193974472	25.364128355034982	26.34065210534351	24.296677599876794
38-39	24.1380956864433	25.532704749351016	25.831304558354738	24.497895005850943
40-41	24.468909303670625	25.440098457234324	26.143892826879362	23.947099412215692
42-43	24.315507014405423	25.546894965499618	26.291847687196523	23.845750332898437
44-45	24.547201422644065	25.17182771963312	26.122723228067628	24.15824762965519
46-47	24.29227758064603	25.236109391069306	25.797508601704727	24.67410442657994
48-49	23.972410193916847	25.266018618276902	26.22369909464223	24.53787209316402
50-51	24.278518299326134	25.46417474814048	25.857734676584126	24.399572275949268
52-53	24.420554965230608	25.73755497868105	25.536807134114355	24.305082921973987
54-55	24.402598625364842	25.127644693128172	25.867957012387855	24.60179966911913
56-57	24.52522833967797	25.350546804589662	25.616618016975828	24.50760683875654
58-59	24.693500109998865	25.450709408569416	25.790528526035416	24.065261955396302
60-61	24.449265564087817	25.785814472104217	25.537784684311966	24.227135279496004
62-63	24.12430898355011	25.400217897157923	26.023914885603993	24.451558233687976
64-65	24.407911549894415	25.20525374258544	25.676355468277134	24.710479239243007
66-67	24.161970220721752	25.39967987948404	25.576418685353815	24.861931214440393
68-69	24.422774288135365	25.34796293058227	25.990423285404923	24.23883949587744
70-71	24.655265175461015	25.19207230957537	25.825386363942055	24.32727615102156
72-73	24.24549746459171	25.295909720634324	25.93332615976435	24.525266655009617
74-75	24.479871413775943	25.117624113952143	25.90319717002704	24.49930730224488
76-77	24.46510739548628	24.704391721488648	25.783457772839952	25.04704311018512
78-79	24.42282315244894	24.638184134312073	25.786417326806593	25.152575386432392
80-81	24.012157237006264	25.39642506209432	25.776826326242507	24.814591374656906
82-83	23.945710815467617	25.526152612840182	26.063166559734647	24.464970011957554
84-85	24.25500491294239	25.073156099749205	26.092263780282188	24.579575207026213
86-87	23.95342111988379	25.812339435350452	25.81671082895073	24.41752861581503
88-89	24.450347693921746	25.073573916903168	25.339825413264826	25.136252975910256
90-91	24.327746916486205	25.213727520949064	25.511587555651204	24.946938006913527
92-93	24.262781282365125	25.29066404831399	26.009590165036922	24.436964504283964
94-95	24.25262619877063	25.097717460018558	25.789137423164348	24.86051891804646
96-97	24.41463677081792	25.532166731677137	25.73116601880372	24.322030478701226
98-99	23.268053855569153	25.768356490510712	26.087400971121898	24.87618868279823
100-101	24.013133986039588	25.353182817871268	25.700577959992355	24.933105236096793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	2.5
3	4.5
4	3.0
5	2.5
6	4.0
7	15.0
8	30.0
9	31.5
10	32.0
11	36.0
12	39.5
13	40.5
14	49.0
15	65.0
16	73.0
17	79.0
18	83.0
19	95.5
20	108.5
21	130.5
22	172.5
23	223.5
24	302.0
25	400.5
26	560.0
27	783.0
28	1065.5
29	1562.5
30	2372.5
31	3580.5
32	5373.0
33	7425.0
34	9434.0
35	11354.0
36	13394.0
37	15249.0
38	16632.5
39	17632.0
40	18164.0
41	19002.0
42	20215.0
43	21660.5
44	23145.0
45	25184.5
46	27146.5
47	28278.5
48	29662.0
49	32381.0
50	35409.5
51	35316.0
52	33893.0
53	34361.5
54	35543.5
55	35109.0
56	30807.0
57	26460.0
58	25140.0
59	23809.0
60	19770.5
61	14867.0
62	11889.5
63	8654.5
64	5342.0
65	3451.0
66	2583.0
67	2194.5
68	1651.0
69	1146.0
70	811.0
71	572.5
72	438.0
73	319.5
74	227.5
75	156.5
76	100.5
77	61.0
78	40.5
79	21.0
80	12.0
81	10.0
82	6.0
83	3.0
84	2.5
85	1.5
86	0.5
87	1.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4826018534708865
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.001076035347761174
22-23	0.0012105397662313209
24-25	3.362610461753669E-4
26-27	0.002555583950932788
28-29	0.0041023847633394755
30-31	0.001076035347761174
32-33	2.690088369402935E-4
34-35	0.0030936016248133747
36-37	0.0022193229047574215
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008810039409794612
46-47	0.07989562457126717
48-49	9.415309292910273E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008070265108208805
58-59	0.03772848938087616
60-61	0.02858218892490618
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	4.035132554104403E-4
78-79	0.009011796037499832
80-81	0.00598544662192153
82-83	9.415309292910273E-4
84-85	0.0034298626709887417
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06886626225671513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	743470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10923114466938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7664060886599	46.55821703929493
2	13.236573890689652	15.648074113365409
3	3.969072787351571	7.0382652245274375
4	1.5117772860678163	3.574399721657741
5	0.7712008217603858	2.2792543816196815
6	0.404533574479631	1.4347001135817503
7	0.2515315529192269	1.0407485691176157
8	0.17634648969135786	0.8338964336574023
9	0.11888693481777281	0.632458377920248
>10	0.645622811727921	7.080549420229923
>50	0.06833005657569682	2.838559625383584
>100	0.07379581858365607	8.480617038579949
>500	0.0050108271869069194	1.996025339760265
>1k	9.110594885285309E-4	0.564234601304146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1081	0.14539927636622862	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1054	0.14176765706753466	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1049	0.14109513497518394	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1007	0.13544594939943777	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	936	0.12589613568805735	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	878	0.11809487941678884	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	877	0.11796037499831868	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	792	0.10652749942835621	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	773	0.10397191547742343	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	747	0.10047480059719961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.3450441847014674E-4
6	0.0	0.0	0.0	0.0	1.3450441847014674E-4
7	0.0	0.0	0.0	0.0	1.3450441847014674E-4
8	0.0	0.0	0.0	0.0	1.3450441847014674E-4
9	0.0	0.0	0.0	0.0	1.3450441847014674E-4
10-11	0.0	0.0	0.0	0.0	1.3450441847014674E-4
12-13	0.0	0.0	0.0	0.0	4.0351325541044023E-4
14-15	0.0	0.0	0.0	0.0	4.0351325541044023E-4
16-17	0.0	0.0	0.0	0.0	4.0351325541044023E-4
18-19	0.0	0.0	0.0	0.0	4.0351325541044023E-4
20-21	0.0	0.0	0.0	0.0	4.0351325541044023E-4
22-23	0.0	0.0	0.0	1.3450441847014674E-4	4.0351325541044023E-4
24-25	0.0	0.0	0.0	5.38017673880587E-4	4.0351325541044023E-4
26-27	0.0	0.0	0.0	6.052698831156603E-4	4.0351325541044023E-4
28-29	0.0	0.0	0.0	0.0014122963939365409	4.0351325541044023E-4
30-31	0.0	0.0	0.0	0.0041696369725745495	4.0351325541044023E-4
32-33	0.0	0.0	0.0	0.014593729404010922	4.0351325541044023E-4
34-35	0.0	0.0	0.0	0.024614308580036853	5.38017673880587E-4
36-37	0.0	0.0	0.0	0.04263790065503652	5.38017673880587E-4
38-39	0.0	0.0	0.0	0.07041306306912182	5.38017673880587E-4
40-41	0.0	0.0	0.0	0.10578772512677041	5.38017673880587E-4
42-43	0.0	0.0	0.0	0.13793428114113548	5.38017673880587E-4
44-45	0.0	0.0	0.0	0.17431772633731019	5.38017673880587E-4
46-47	0.0	0.0	0.0	0.2141982864137087	5.38017673880587E-4
48-49	0.0	0.0	0.0	0.25818123125344666	5.38017673880587E-4
50-51	0.0	0.0	0.0	0.30391273353329656	5.38017673880587E-4
52-53	0.0	0.0	0.0	0.35267058522872474	5.38017673880587E-4
54-55	0.0	0.0	0.0	0.40418577750279094	5.38017673880587E-4
56-57	0.0	0.0	0.0	0.4637039826758309	6.052698831156603E-4
58-59	0.0	0.0	0.0	0.5308216874924341	6.725220923507338E-4
60-61	0.0	0.0	0.0	0.602243533700082	6.725220923507338E-4
62-63	0.0	0.0	0.0	0.6756829461847822	6.725220923507338E-4
64-65	0.0	0.0	0.0	0.7517451948296501	6.725220923507338E-4
66-67	0.0	0.0	0.0	0.8409888764845925	7.397743015858071E-4
68-69	0.0	0.0	0.0	0.9424052080110832	8.070265108208805E-4
70-71	0.0	0.0	0.0	1.048529194184029	8.070265108208805E-4
72-73	0.0	0.0	0.0	1.1712644760380377	8.070265108208805E-4
74-75	0.0	0.0	0.0	1.3080554696221771	8.070265108208805E-4
76-77	0.0	0.0	0.0	1.4434341668123798	8.070265108208805E-4
78-79	0.0	0.0	0.0	1.5989212745638692	8.070265108208805E-4
80-81	0.0	0.0	0.0	1.7673880586977282	8.070265108208805E-4
82-83	0.0	0.0	0.0	1.9534749216511762	8.070265108208805E-4
84-85	0.0	0.0	0.0	2.1450764657619006	8.070265108208805E-4
86-87	0.0	0.0	0.0	2.35106998264893	0.0010087831385261006
88-89	0.0	0.0	0.0	2.5769701534695413	0.0012105397662313206
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1230	0.0	49.639263	1
GGTATCA	605	0.0	48.88288	1
ATCAACG	1570	0.0	38.11179	3
TCAACGC	1630	0.0	36.7089	4
CAACGCA	1830	0.0	32.697002	5
ACCGTGC	235	0.0	32.332596	8
TATCAAC	1930	0.0	31.002855	2
AACGCAG	1945	0.0	30.76376	6
CGCAGAG	1980	0.0	30.699638	8
CCGTGCG	190	1.2732926E-11	29.992735	9
ACGCAGA	2040	0.0	29.331133	7
ACCGTAT	135	4.2306783E-7	28.141335	8
CGTTACG	35	0.008378862	27.151808	52-53
GGACCGT	125	7.400213E-6	26.59356	6
AACCGTG	215	5.820766E-11	26.50521	7
GTACATG	2955	0.0	26.473225	1
GTCTAGG	205	9.094947E-10	25.595243	1
GACCGCA	570	0.0	24.993948	7
GACCGTA	95	0.0012278793	24.993946	7
TACATGG	2910	0.0	24.96818	2
>>END_MODULE