FastQCFastQC Report
Fri 10 Feb 2017
SRR3224494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240347
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA4190.17433127935859405No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4030.1676742376647098No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3840.15976900065322222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.1452067219478504No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT3390.14104607088917273No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3130.1302283781366108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.12149101091338774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5850.051.261091
GGTATCA2900.050.8823171
GGATAGG554.890696E-543.272351
ACCGTGC853.630321E-739.1099328
ATCAACG7600.038.7423633
TCAACGC7950.037.0367244
CCGTGCG905.6725E-736.944859
TATCAAC8200.035.9075552
TACACTA801.0755199E-535.6179775
TGGTATC1601.8189894E-1235.6179772
AAACCGT958.67527E-734.99316
TCTAATG2200.034.5386472
GTCTAAT1950.034.174061
GTTATTA1950.034.095848
AGTTGCG702.0508932E-433.9218832
CAACGCA8850.033.2702755
AACCGTG1151.0323856E-733.0369647
AACGCAG9150.032.698476
ACTACTC753.074812E-431.6604278
TACACCG753.074812E-431.6604275
Produced by FastQC (version 0.11.5)