##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224494_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240347
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09041094750506	34.0	31.0	34.0	30.0	34.0
2	32.257868831314724	34.0	31.0	34.0	31.0	34.0
3	32.40411155537618	34.0	31.0	34.0	31.0	34.0
4	35.68537572759385	37.0	35.0	37.0	35.0	37.0
5	35.70180197797351	37.0	37.0	37.0	35.0	37.0
6	35.815292056901065	37.0	37.0	37.0	35.0	37.0
7	35.77998477201712	37.0	37.0	37.0	35.0	37.0
8	35.80467823605038	37.0	37.0	37.0	35.0	37.0
9	37.5164574552626	39.0	38.0	39.0	35.0	39.0
10-11	37.5963481965658	39.0	38.5	39.0	35.0	39.0
12-13	37.59845556632702	39.0	39.0	39.0	35.0	39.0
14-15	39.08161533116702	41.0	40.0	41.0	36.0	41.0
16-17	38.92684743308633	41.0	39.5	41.0	35.5	41.0
18-19	39.022242840559684	41.0	39.5	41.0	36.0	41.0
20-21	39.07906277174253	41.0	40.0	41.0	36.0	41.0
22-23	39.038165652161254	41.0	40.0	41.0	36.0	41.0
24-25	38.98190324822028	41.0	39.5	41.0	35.5	41.0
26-27	38.80164928207966	41.0	39.0	41.0	35.0	41.0
28-29	38.712193203992555	41.0	39.0	41.0	35.0	41.0
30-31	38.60692873220802	41.0	39.0	41.0	35.0	41.0
32-33	38.39047294120584	40.0	38.5	41.0	34.0	41.0
34-35	38.382461607592354	40.0	38.0	41.0	34.0	41.0
36-37	38.38359954565691	40.0	38.0	41.0	34.0	41.0
38-39	38.27028421407382	40.0	38.0	41.0	34.0	41.0
40-41	38.15897015564995	40.0	38.0	41.0	33.5	41.0
42-43	37.997420396343614	40.0	38.0	41.0	33.0	41.0
44-45	37.79159298847084	40.0	38.0	41.0	33.0	41.0
46-47	37.57329402904966	40.0	37.0	41.0	32.0	41.0
48-49	37.32177851190154	40.0	37.0	41.0	31.5	41.0
50-51	36.05025858446329	38.5	35.5	40.0	29.5	40.5
52-53	36.40673692619421	39.0	35.5	40.0	30.5	40.5
54-55	36.887974470245105	40.0	36.0	41.0	31.0	41.0
56-57	36.75018826946041	39.0	35.0	41.0	31.0	41.0
58-59	36.609537460421805	39.0	35.0	41.0	31.0	41.0
60-61	36.3744377920257	39.0	35.0	41.0	30.5	41.0
62-63	36.078492762547484	38.5	35.0	41.0	30.0	41.0
64-65	35.83313916961726	37.5	35.0	41.0	29.0	41.0
66-67	35.56363091696589	37.0	35.0	40.0	29.0	41.0
68-69	35.251550882682125	36.5	35.0	40.0	29.0	41.0
70-71	34.92701593945421	36.0	35.0	39.0	28.5	41.0
72-73	34.60599674637088	35.5	34.0	39.0	28.0	41.0
74-75	34.31480318040167	35.0	34.0	38.5	28.0	40.5
76-77	33.962697682933424	35.0	34.0	37.0	27.0	39.5
78-79	33.697949215093175	35.0	34.0	37.0	27.0	39.0
80-81	33.39170241359368	35.0	34.0	36.5	27.0	39.0
82-83	33.12640473981368	35.0	34.0	36.0	26.0	38.0
84-85	32.89789138204346	35.0	34.0	36.0	26.0	37.0
86-87	32.671308566364466	35.0	33.0	35.0	26.0	37.0
88-89	32.52005849875388	35.0	33.0	35.0	25.5	36.0
90-91	32.32942162789634	35.0	33.0	35.0	25.0	36.0
92-93	32.190545336534264	35.0	33.0	35.0	25.0	36.0
94-95	32.05521391987418	35.0	33.0	35.0	25.0	36.0
96-97	31.92124511643582	35.0	33.0	35.0	24.0	35.0
98-99	31.859567625141985	35.0	33.0	35.0	24.0	35.0
100-101	30.685461020940558	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	37.0
8	169.0
9	118.0
10	145.0
11	278.0
12	499.0
13	567.0
14	588.0
15	576.0
16	586.0
17	647.0
18	802.0
19	964.0
20	1091.0
21	1563.0
22	1930.0
23	1673.0
24	1525.0
25	1428.0
26	1514.0
27	1607.0
28	1770.0
29	2049.0
30	2571.0
31	3092.0
32	3945.0
33	5511.0
34	8613.0
35	15127.0
36	31430.0
37	56857.0
38	69053.0
39	22015.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52763689253222	19.378123033452663	13.958683231159696	23.13555684285542
2	15.545856615643217	19.366582482826914	40.14154534901622	24.946015552513657
3	20.281925715735998	25.167362188835305	28.34901205340612	26.201700042022573
4	12.95085855034596	16.63844358365197	38.81721011703911	31.593487748962957
5	13.802127756951407	38.23347077350664	33.84980881808385	14.1145926514581
6	29.989972830948584	37.12632152679251	18.72417795936708	14.159527682891818
7	27.531860185481825	31.6484083429375	21.51098203846938	19.308749433111295
8	26.035273999675468	35.050156648512356	19.874597977091454	19.039971374720714
9	26.07022346856836	16.68878746146197	20.38968657815575	36.85130249181392
10-11	25.89880464495084	25.83015390248266	26.99097554785373	21.28006590471277
12-13	26.371246572663694	23.144245611553295	27.589901267750378	22.894606548032638
14-15	23.202423959911435	25.054938651838782	25.21642499209216	26.52621239615762
16-17	22.801816917651664	28.118091320512768	26.061568694392285	23.018523067443283
18-19	22.850008843204776	27.08767257254029	27.622010216502463	22.440308367752472
20-21	23.9223675968376	25.564335377642184	27.823966070161344	22.689330955358873
22-23	23.538472746953094	26.387943506000145	27.146886481198155	22.926697265848606
24-25	22.95778135188726	26.138068357964865	27.605140891811985	23.29900939833589
26-27	23.02233853553404	26.743832874968277	27.580331770315418	22.653496819182266
28-29	22.865690023174825	26.824341472953687	27.55744818949269	22.752520314378792
30-31	23.040229335086355	26.130552908919185	27.72553849226327	23.103679263731188
32-33	22.670139423416977	26.340873819935346	27.886555688234093	23.102431068413583
34-35	23.332723104511395	26.27867208660811	27.529363794846617	22.859241014033877
36-37	22.957676141329227	26.214782138249614	27.877148445628197	22.950393274792958
38-39	22.928733106983557	26.47481026562812	27.466754876506222	23.1297017508821
40-41	23.56439969209645	25.923398589468867	27.752928204381384	22.759273514053298
42-43	23.277759516686707	26.314070424937903	27.59183826313446	22.81633179524093
44-45	23.3373279904794	26.24843438567582	27.26998472875862	23.144252895086158
46-47	23.371209126804164	26.18692973076427	26.93085414005584	23.51100700237573
48-49	23.143621513894495	26.34004168972361	27.11433885174352	23.401997944638378
50-51	23.185644089587136	26.645017412324677	26.826421798483025	23.342916699605155
52-53	23.474809338165237	26.65042625870096	26.2485073664327	23.626257036701105
54-55	23.23619599995007	26.440937477896544	26.64376921700708	23.67909730514631
56-57	23.303230960301065	26.908650635644786	26.335103694041727	23.45301471001242
58-59	23.686701717081977	26.642338044403203	26.61008944305625	23.060870795458566
60-61	23.518176370718066	26.70153800059922	26.594593694863345	23.18569193381937
62-63	23.357333111370068	26.569835192275676	26.689487264857664	23.383344431496585
64-65	23.97405959799037	26.1568495231911	26.200347148512133	23.6687437303064
66-67	23.245357607282184	26.36421326397919	26.314902470741224	24.075526657997397
68-69	23.456502473507054	26.32319105293596	26.90817859178604	23.312127881770937
70-71	23.503725863023046	26.083745584509067	26.840151946976665	23.572376605491225
72-73	23.316704597935484	26.21959084157489	26.841816207400132	23.62188835308949
74-75	23.45234182244838	26.272015044914227	26.810611324460055	23.465031808177343
76-77	23.43549118566073	25.937498699796546	26.39142573029828	24.235584384244447
78-79	23.439379122365327	25.62327042716548	26.800940471484157	24.13640997898504
80-81	23.422341464023827	26.39251690627192	26.31113368287185	23.8740079468324
82-83	23.345529318231957	26.49192692398946	26.64219144782574	23.520352309952838
84-85	23.279675723815902	26.151842156736265	26.89740271090229	23.67107940854554
86-87	22.87793922666883	26.88675612614244	26.70130151256866	23.534003134620068
88-89	23.44911111712278	26.140211051156285	26.411776579606155	23.998901252114777
90-91	22.9176981614083	26.263277677691004	26.770044976637948	24.048979184262752
92-93	22.96117383462213	26.35656495680087	27.043090948050786	23.639170260526214
94-95	23.16380067152908	26.19608316309336	26.84056801208253	23.799548153295028
96-97	23.2842515196778	26.574494376880093	26.70617898288724	23.435075120554867
98-99	22.52731478784752	26.867515997769882	26.79095969976617	23.814209514616426
100-101	23.078459937565036	26.273257023933404	26.578772112382936	24.069510926118625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.5
4	1.5
5	1.0
6	4.5
7	8.5
8	15.5
9	24.0
10	27.0
11	21.5
12	27.5
13	44.0
14	48.5
15	49.5
16	65.0
17	84.0
18	81.5
19	92.0
20	113.0
21	111.5
22	145.0
23	207.5
24	270.0
25	352.5
26	497.5
27	659.5
28	836.5
29	1099.5
30	1498.5
31	2081.0
32	2964.5
33	4169.0
34	5414.5
35	6464.0
36	7322.5
37	7918.0
38	7817.0
39	7442.0
40	7150.0
41	6897.5
42	6649.0
43	6468.5
44	6707.5
45	7312.0
46	7893.0
47	8175.0
48	8489.5
49	9010.0
50	9757.0
51	9861.5
52	9511.5
53	9760.0
54	9928.5
55	9683.0
56	8545.0
57	7303.0
58	6651.5
59	5941.5
60	4930.5
61	3868.0
62	3217.5
63	2421.0
64	1602.5
65	1132.0
66	899.0
67	734.5
68	548.5
69	378.0
70	256.5
71	186.5
72	129.5
73	97.5
74	70.0
75	48.0
76	40.0
77	29.0
78	21.0
79	15.5
80	7.0
81	6.0
82	6.5
83	3.0
84	3.0
85	3.5
86	2.5
87	2.0
88	1.0
89	0.5
90	0.5
91	1.0
92	1.0
93	1.0
94	1.5
95	1.5
96	0.5
97	0.5
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16476178192363541
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03286914336355353
16-17	0.06657041693884258
18-19	0.020595222740454427
20-21	0.03598963165756177
22-23	0.06012140779789221
24-25	0.016018506575909
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023715711034462673
38-39	0.004576716164545428
40-41	0.004992781270413194
42-43	0.002496390635206597
44-45	0.010817692752561921
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	2.080325529338831E-4
58-59	0.011025725305495804
60-61	0.014562278705371816
62-63	0.027876362093140337
64-65	0.043270771010247684
66-67	0.014354246152437935
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01747473444644618
80-81	0.05221617078640466
82-83	0.043270771010247684
84-85	0.02413177614033044
86-87	0.05263223589227242
88-89	0.030580785281280817
90-91	0.0
92-93	0.0027044231881404803
94-95	0.0
96-97	0.0
98-99	4.160651058677662E-4
100-101	0.04035831526917332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	240347.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.18939419926475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15603172654325	59.77050279299441
2	9.772444879382409	13.718439718591165
3	2.3064401616586396	4.856629131110226
4	0.9079204588993663	2.5490554796505993
5	0.45411647624752144	1.593708018185917
6	0.2906533412749741	1.2240469167649553
7	0.18671524199125764	0.9173800806194846
8	0.139896518226899	0.7855401491945952
9	0.09575513035445347	0.6048895131945645
>10	0.6115782904110827	8.338037032844388
>50	0.05705292728013569	2.7943974156544473
>100	0.021394847730050882	2.8473737511952915
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	419	0.17433127935859405	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	403	0.1676742376647098	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	384	0.15976900065322222	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.1452067219478504	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	339	0.14104607088917273	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	313	0.1302283781366108	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	292	0.12149101091338774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	6.240976588016493E-4	0.0
22-23	0.0	0.0	0.0	8.321302117355324E-4	0.0
24-25	0.0	0.0	0.0	0.0012481953176032986	0.0
26-27	0.0	0.0	0.0	0.0016642604234710647	0.0
28-29	0.0	0.0	0.0	0.002080325529338831	0.0
30-31	0.0	0.0	0.0	0.0031204882940082464	0.0
32-33	0.0	0.0	0.0	0.006865074246818143	0.0
34-35	0.0	0.0	0.0	0.011857855517231336	0.0
36-37	0.0	0.0	0.0	0.02288358082272714	0.0
38-39	0.0	0.0	0.0	0.03765389208103284	0.0
40-41	0.0	0.0	0.0	0.054088463762809603	0.0
42-43	0.0	0.0	0.0	0.07572384926793345	0.0
44-45	0.0	0.0	0.0	0.10110382072586718	0.0
46-47	0.0	0.0	0.0	0.12440346665446209	0.0
48-49	0.0	0.0	0.0	0.14624688471251981	0.0
50-51	0.0	0.0	0.0	0.16975456319404858	0.0
52-53	0.0	0.0	0.0	0.20512009719280871	0.0
54-55	0.0	0.0	0.0	0.2375731754504945	0.0
56-57	0.0	0.0	0.0	0.2754351000844612	0.0
58-59	0.0	0.0	0.0	0.32016209896524606	0.0
60-61	0.0	0.0	0.0	0.3692577814576425	0.0
62-63	0.0	0.0	0.0	0.41377674778549345	0.0
64-65	0.0	0.0	0.0	0.4659929185718981	0.0
66-67	0.0	0.0	0.0	0.5306910425343359	0.0
68-69	0.0	0.0	0.0	0.6111996405197486	0.0
70-71	0.0	0.0	0.0	0.690460043187558	0.0
72-73	0.0	0.0	0.0	0.7919799290192929	0.0
74-75	0.0	0.0	0.0	0.8993247263331766	0.0
76-77	0.0	0.0	0.0	1.0062534585411926	0.0
78-79	0.0	0.0	0.0	1.1250400462664398	0.0
80-81	0.0	0.0	0.0	1.2617174335440011	0.0
82-83	0.0	0.0	0.0	1.4050518625154464	0.0
84-85	0.0	0.0	0.0	1.5550433331807763	0.0
86-87	0.0	0.0	0.0	1.7081552921401142	0.0
88-89	0.0	0.0	0.0	1.9026657291332947	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	585	0.0	51.26109	1
GGTATCA	290	0.0	50.882317	1
GGATAGG	55	4.890696E-5	43.27235	1
ACCGTGC	85	3.630321E-7	39.109932	8
ATCAACG	760	0.0	38.742363	3
TCAACGC	795	0.0	37.036724	4
CCGTGCG	90	5.6725E-7	36.94485	9
TATCAAC	820	0.0	35.907555	2
TACACTA	80	1.0755199E-5	35.617977	5
TGGTATC	160	1.8189894E-12	35.617977	2
AAACCGT	95	8.67527E-7	34.9931	6
TCTAATG	220	0.0	34.538647	2
GTCTAAT	195	0.0	34.17406	1
GTTATTA	195	0.0	34.09584	8
AGTTGCG	70	2.0508932E-4	33.921883	2
CAACGCA	885	0.0	33.270275	5
AACCGTG	115	1.0323856E-7	33.036964	7
AACGCAG	915	0.0	32.69847	6
ACTACTC	75	3.074812E-4	31.660427	8
TACACCG	75	3.074812E-4	31.660427	5
>>END_MODULE