FastQCFastQC Report
Fri 10 Feb 2017
SRR3224494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240347
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.16933849808818083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.14936737300652805No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG3590.14936737300652805No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT3520.1464549172654537No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3070.12773198750140421No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT2740.11400183900776793No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA2580.10734479731388369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTT407.5334556E-659.366946
GTCGACA250.00501915556.992268
GGTATCA3600.053.0243721
CCGTGCG502.822536E-547.493559
GTATCAA7100.044.3612371
AAACCGT554.9555194E-543.1759536
ATCAACG8550.037.2171673
TCAACGC8650.036.786914
ATAGCGT651.3261191E-436.53355
ACCGTGC651.3261191E-436.53358
TGGTATC2350.036.378042
CAACGCA9150.034.7766955
TATCAAC9350.034.032812
AACGCAG9700.033.2944456
CCGATAT300.003951310631.66236970-71
CGTTTAA902.4073193E-531.6623699
CGCAGAG10200.031.6623678
ACGCAGA10350.031.2034917
GTGCGCG552.7319784E-630.22316710-11
TGCGCGG404.493365E-429.6834712-13
Produced by FastQC (version 0.11.5)