##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240347
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13067772845095	34.0	33.0	34.0	31.0	34.0
2	33.254365563123315	34.0	33.0	34.0	31.0	34.0
3	33.37741265753265	34.0	34.0	34.0	31.0	34.0
4	36.596862036971544	37.0	37.0	37.0	35.0	37.0
5	36.630854556120944	37.0	37.0	37.0	35.0	37.0
6	36.6692615260436	37.0	37.0	37.0	35.0	37.0
7	36.64963573499981	37.0	37.0	37.0	35.0	37.0
8	36.636296687705695	37.0	37.0	37.0	35.0	37.0
9	38.45941076859707	39.0	39.0	39.0	37.0	39.0
10-11	38.493278468214704	39.0	39.0	39.0	37.0	39.0
12-13	38.49059276795633	39.0	39.0	39.0	37.0	39.0
14-15	40.069861491926254	41.0	40.0	41.0	38.0	41.0
16-17	40.08326710963732	41.0	40.0	41.0	38.0	41.0
18-19	40.07995107074355	41.0	40.0	41.0	38.0	41.0
20-21	40.0901155412799	41.0	40.0	41.0	38.0	41.0
22-23	40.01320382613471	41.0	40.0	41.0	38.0	41.0
24-25	39.9746720366803	41.0	40.0	41.0	38.0	41.0
26-27	39.907213320740425	41.0	40.0	41.0	38.0	41.0
28-29	39.87046228993913	41.0	40.0	41.0	38.0	41.0
30-31	39.78693721993618	41.0	40.0	41.0	37.5	41.0
32-33	39.711635676750696	41.0	40.0	41.0	37.0	41.0
34-35	39.61883651553795	41.0	40.0	41.0	37.0	41.0
36-37	39.50765143729691	41.0	40.0	41.0	36.0	41.0
38-39	39.40064365271878	41.0	39.5	41.0	36.0	41.0
40-41	39.25728217951546	41.0	39.0	41.0	35.0	41.0
42-43	39.137779959808114	41.0	39.0	41.0	35.0	41.0
44-45	38.87113839573617	41.0	39.0	41.0	35.0	41.0
46-47	38.600673193341294	40.5	38.0	41.0	35.0	41.0
48-49	38.46039268224692	40.0	38.0	41.0	35.0	41.0
50-51	38.30544795649624	40.0	38.0	41.0	35.0	41.0
52-53	38.082601405467926	40.0	37.0	41.0	34.5	41.0
54-55	37.898080691666635	40.0	37.0	41.0	34.0	41.0
56-57	37.68595821874207	40.0	36.0	41.0	34.0	41.0
58-59	37.42862611141391	39.5	35.5	41.0	33.5	41.0
60-61	37.18843588644751	39.0	35.0	41.0	33.0	41.0
62-63	36.97463875147183	39.0	35.0	41.0	33.0	41.0
64-65	36.69189338747728	38.0	35.0	41.0	33.0	41.0
66-67	36.38650784074692	37.0	35.0	40.0	32.5	41.0
68-69	36.1223709886123	37.0	35.0	40.0	32.0	41.0
70-71	35.80942553890833	36.5	35.0	39.5	32.0	41.0
72-73	35.49229863489039	36.0	35.0	39.0	31.5	41.0
74-75	35.15386919745201	35.5	35.0	39.0	31.0	40.5
76-77	33.3043745085231	34.5	32.0	36.5	28.5	39.0
78-79	34.60517917843784	35.0	34.5	37.0	31.0	39.0
80-81	34.520050177451765	35.0	35.0	37.0	32.0	39.0
82-83	34.302304584621396	35.0	35.0	36.0	31.5	38.0
84-85	34.0802880834793	35.0	35.0	36.0	31.0	37.0
86-87	33.86996509213762	35.0	34.5	36.0	31.0	37.0
88-89	33.694229593046714	35.0	34.0	35.0	31.0	36.5
90-91	33.550551910362934	35.0	34.0	35.0	31.0	36.0
92-93	33.44679983523822	35.0	34.0	35.0	31.0	36.0
94-95	33.32948195733668	35.0	34.0	35.0	30.5	36.0
96-97	33.27055881704369	35.0	34.0	35.0	30.0	36.0
98-99	33.22178558500835	35.0	34.0	35.0	30.0	35.0
100-101	32.40497489047086	34.5	33.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	14.0
16	39.0
17	88.0
18	188.0
19	301.0
20	475.0
21	894.0
22	1273.0
23	1196.0
24	935.0
25	805.0
26	955.0
27	1145.0
28	1389.0
29	1634.0
30	1830.0
31	2140.0
32	2753.0
33	3717.0
34	5906.0
35	11488.0
36	28940.0
37	62394.0
38	82027.0
39	27810.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93404792561997	19.36046219962877	13.827109914549924	22.878379960201332
2	15.548353006278424	19.528431809009476	40.44402468098208	24.479190503730024
3	20.219515949855836	25.327963319700263	28.825822664730577	25.62669806571332
4	12.872638310442818	16.92927309265354	39.08473998011209	31.11334861679155
5	13.627796477592813	37.643074388280276	34.528827070860046	14.200302063266859
6	29.91716143742173	37.04685309157177	18.885195155337907	14.150790315668596
7	27.492334000424385	31.614291004256344	21.735241130532106	19.15813386478716
8	26.04026678094588	34.70523867574798	19.894569102173108	19.359925441133026
9	26.196707260752163	16.812774863010564	20.542382472009223	36.448135404228054
10-11	25.94686016467857	25.738619579191752	27.234581667339302	21.079938588790373
12-13	26.48316808614212	23.474601305612303	27.551415245457612	22.49081536278797
14-15	23.476265566035774	25.051488056851134	25.550766183892453	25.921480193220635
16-17	22.708001348050942	28.01283144786496	26.500434788035633	22.778732416048463
18-19	22.51120255297549	27.06441103903939	28.219823838034173	22.204562569950944
20-21	23.784143626869707	25.76629428529822	28.19571865443425	22.253843433397826
22-23	23.657133109210132	26.2032753053956	27.473745964118095	22.66584562127617
24-25	23.1332198862478	26.14573928528336	27.73094733863955	22.990093489829288
26-27	23.049094317091996	26.807673184421464	27.60840214110221	22.53483035738433
28-29	22.91772420507785	26.718829315380837	27.939402008837554	22.42404447070376
30-31	22.88774147378582	26.086450007697202	28.205261559328804	22.820546959188174
32-33	22.84327002889578	26.268117072643037	28.232156490733168	22.656456407728008
34-35	23.343915520031956	26.3173990288798	27.70272238194898	22.635963069139265
36-37	22.853380377210904	26.01719209629571	28.10857815705055	23.02084936944284
38-39	22.853832167657593	26.20253217223431	27.695373772087855	23.248261888020238
40-41	23.079963552696725	26.27575963086704	28.00034949468893	22.643927321747306
42-43	23.11012827287214	26.360220847358196	27.872617507187524	22.65703337258214
44-45	23.417610905595527	26.108847896736393	27.491812897131705	22.981728300536375
46-47	23.162834858144656	26.13965245128863	27.338957853576467	23.35855483699024
48-49	22.84274097081077	25.976562906316115	27.559076412913907	23.621619709959205
50-51	23.267608915443088	26.318614336771418	27.017811747182197	23.395965000603294
52-53	23.43757151119007	26.707219145651912	26.67622229526476	23.178987047893255
54-55	23.555525968703584	26.1858895679996	26.95082526513749	23.307759198159328
56-57	23.57691947614243	26.553628826729636	26.61666649329575	23.25278520383219
58-59	23.840522103230263	26.450205819553062	26.820431284572066	22.888840792644604
60-61	23.575056756346203	26.597345197195786	26.793989549757264	23.033608496700744
62-63	23.514751588328544	26.27471946810237	27.008450282300174	23.202078661268917
64-65	23.626881134359902	26.201283976916706	26.70763521075778	23.46419967796561
66-67	23.263656296937345	26.318406304218485	26.671021481441414	23.746915917402756
68-69	23.718415457650813	26.112662109366873	26.901105484986292	23.267816947996025
70-71	23.750036405696765	26.05337283178072	26.95311362321976	23.243477139302758
72-73	23.271353501395897	26.254124245361915	26.85492225823497	23.619599995007217
74-75	23.53222632277499	26.127016355519313	26.81040329190712	23.530354029798584
76-77	23.493630016725888	25.803633095620484	26.821540612283957	23.881196275369675
78-79	23.36703699080431	25.665335164149294	26.88220363666625	24.085424208380143
80-81	23.00705265566813	26.48136975471737	26.846485114527642	23.66509247508686
82-83	23.282926311716356	26.411989506685224	27.001978418388685	23.30310576320974
84-85	23.345288419931432	26.03643111540126	27.137353127184372	23.48092733748294
86-87	22.836981530869952	26.702018331828565	26.944168223443604	23.51683191385788
88-89	23.49061981218821	25.871552380516505	26.594049436855876	24.043778370439405
90-91	23.195837684680896	26.370830507557823	26.540377038198937	23.89295476956234
92-93	23.22579437230338	26.358140521828854	27.01801977973513	23.39804532613263
94-95	23.24846992057317	26.231864762197986	26.736967800721455	23.782697516507383
96-97	23.15194281601185	26.5769907675153	26.87530944842249	23.39575696805036
98-99	22.440263452425036	26.871564862469675	26.90089745243336	23.78727423267193
100-101	23.048980280071692	26.433821769152143	26.796037279053742	23.72116067172242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	3.0
4	3.0
5	1.0
6	3.5
7	11.5
8	19.0
9	31.0
10	40.0
11	36.5
12	37.5
13	47.5
14	53.0
15	57.5
16	68.0
17	78.5
18	91.5
19	108.0
20	115.5
21	133.5
22	177.5
23	233.5
24	308.5
25	408.0
26	555.5
27	723.0
28	901.0
29	1170.5
30	1546.5
31	2089.0
32	3032.5
33	4337.5
34	5616.5
35	6694.5
36	7490.0
37	7829.5
38	7727.0
39	7415.5
40	7040.0
41	6690.0
42	6492.5
43	6597.5
44	6905.0
45	7317.5
46	7763.0
47	8151.5
48	8548.5
49	9303.5
50	10121.0
51	9855.0
52	9250.5
53	9561.5
54	9930.5
55	9583.5
56	8318.5
57	7156.5
58	6701.0
59	6232.5
60	5165.5
61	3849.5
62	3060.0
63	2237.0
64	1444.5
65	982.5
66	745.0
67	592.5
68	437.5
69	320.5
70	222.5
71	154.5
72	118.5
73	99.5
74	73.0
75	49.5
76	34.5
77	20.0
78	7.5
79	5.0
80	3.5
81	2.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4755624160068568
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.321302117355324E-4
22-23	0.0012481953176032986
24-25	0.0
26-27	0.0014562278705371817
28-29	0.0037445859528098956
30-31	0.0
32-33	2.080325529338831E-4
34-35	0.002496390635206597
36-37	0.0016642604234710647
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.012481953176032986
46-47	0.08654154202049537
48-49	6.240976588016493E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.00603294403508261
58-59	0.036613729316363426
60-61	0.027252264434338685
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	4.160651058677662E-4
78-79	0.007073106799752025
80-81	0.004992781270413194
82-83	0.0014562278705371817
84-85	0.0012481953176032986
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06594631928004094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	240347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.43620888529644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.90761460830288	57.42319040400766
2	10.563608257894394	14.458666026394063
3	2.581069780386806	5.29915891914233
4	0.9348864207108966	2.5592032948719217
5	0.5051041154044823	1.7283705375322016
6	0.32157039042845514	1.3204235046412853
7	0.21351081088175594	1.0228309316941009
8	0.14188425986774456	0.7768016676675719
9	0.09735509964981119	0.5996352542114995
>10	0.6506951175557557	8.814046529845621
>50	0.053512501652190604	2.4877941143138127
>100	0.029188637264831237	3.509878815677935
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	407	0.16933849808818083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.14936737300652805	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	359	0.14936737300652805	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	352	0.1464549172654537	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	307	0.12773198750140421	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	274	0.11400183900776793	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	258	0.10734479731388369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	6.240976588016493E-4	0.0
22-23	0.0	0.0	0.0	8.321302117355324E-4	0.0
24-25	0.0	0.0	0.0	0.0012481953176032986	0.0
26-27	0.0	0.0	0.0	0.0016642604234710647	0.0
28-29	0.0	0.0	0.0	0.002080325529338831	0.0
30-31	0.0	0.0	0.0	0.0031204882940082464	0.0
32-33	0.0	0.0	0.0	0.006865074246818143	0.0
34-35	0.0	0.0	0.0	0.01206588807016522	4.160651058677662E-4
36-37	0.0	0.0	0.0	0.023091613375661024	4.160651058677662E-4
38-39	0.0	0.0	0.0	0.038486022292768374	4.160651058677662E-4
40-41	0.0	0.0	0.0	0.055544691633346785	4.160651058677662E-4
42-43	0.0	0.0	0.0	0.07697204458553675	4.160651058677662E-4
44-45	0.0	0.0	0.0	0.10235201604347048	4.160651058677662E-4
46-47	0.0	0.0	0.0	0.1264837921838009	4.160651058677662E-4
48-49	0.0	0.0	0.0	0.14832721024185863	4.160651058677662E-4
50-51	0.0	0.0	0.0	0.17162685617045353	4.160651058677662E-4
52-53	0.0	0.0	0.0	0.20657632506334592	4.160651058677662E-4
54-55	0.0	0.0	0.0	0.23965350097983332	4.160651058677662E-4
56-57	0.0	0.0	0.0	0.27709936050793227	4.160651058677662E-4
58-59	0.0	0.0	0.0	0.3224504570475188	4.160651058677662E-4
60-61	0.0	0.0	0.0	0.371962204645783	4.160651058677662E-4
62-63	0.0	0.0	0.0	0.4171052686324356	4.160651058677662E-4
64-65	0.0	0.0	0.0	0.4689053743129725	4.160651058677662E-4
66-67	0.0	0.0	0.0	0.5342275959342118	4.160651058677662E-4
68-69	0.0	0.0	0.0	0.6153602915784262	4.160651058677662E-4
70-71	0.0	0.0	0.0	0.6956608570109051	4.160651058677662E-4
72-73	0.0	0.0	0.0	0.7994691009249127	4.160651058677662E-4
74-75	0.0	0.0	0.0	0.9072299633446641	4.160651058677662E-4
76-77	0.0	0.0	0.0	1.0149908257644156	4.160651058677662E-4
78-79	0.0	0.0	0.0	1.1346095437013983	4.160651058677662E-4
80-81	0.0	0.0	0.0	1.2737833216141663	4.160651058677662E-4
82-83	0.0	0.0	0.0	1.4169097180326777	4.160651058677662E-4
84-85	0.0	0.0	0.0	1.5669011886980075	4.160651058677662E-4
86-87	0.0	0.0	0.0	1.7210533104220147	4.160651058677662E-4
88-89	0.0	0.0	0.0	1.9188922682621377	4.160651058677662E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTT	40	7.5334556E-6	59.36694	6
GTCGACA	25	0.005019155	56.99226	8
GGTATCA	360	0.0	53.024372	1
CCGTGCG	50	2.822536E-5	47.49355	9
GTATCAA	710	0.0	44.361237	1
AAACCGT	55	4.9555194E-5	43.175953	6
ATCAACG	855	0.0	37.217167	3
TCAACGC	865	0.0	36.78691	4
ATAGCGT	65	1.3261191E-4	36.5335	5
ACCGTGC	65	1.3261191E-4	36.5335	8
TGGTATC	235	0.0	36.37804	2
CAACGCA	915	0.0	34.776695	5
TATCAAC	935	0.0	34.03281	2
AACGCAG	970	0.0	33.294445	6
CCGATAT	30	0.0039513106	31.662369	70-71
CGTTTAA	90	2.4073193E-5	31.662369	9
CGCAGAG	1020	0.0	31.662367	8
ACGCAGA	1035	0.0	31.203491	7
GTGCGCG	55	2.7319784E-6	30.223167	10-11
TGCGCGG	40	4.493365E-4	29.68347	12-13
>>END_MODULE