FastQCFastQC Report
Fri 10 Feb 2017
SRR3224490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844047
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA26160.30993534720222926No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG25890.306736473205876No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24080.285292169748841No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA20210.2394416424677773No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT16270.19276177748395526No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14770.17499025528199258No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA12960.15354595182495762No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA12730.15082098508732333No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC12150.14394932983589778No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11680.13838091954594944No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG11560.13695919776979246No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC11400.1350635687349164No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA11320.13411575421747843No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC10330.12238654956418303No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA10290.12191264230546404No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG10210.12096482778802603No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA10040.11895072193847026No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT9530.11290840438980293No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC9080.10757694772921413No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA8560.10141615336586707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG2100.054.2985849
AAACCGT2250.052.7590146
AACCGTG2350.052.534517
ACCGTGC2700.051.0034038
GGTATCA9150.047.8190731
GTATCAA16750.045.7136081
TTGTACG801.0798969E-535.6123354
ATCAACG22350.034.2048383
TCAACGC23850.032.0535934
GTCTAAT5050.030.1365151
TATCAAC26100.029.1084232
CGCAGAG26650.028.687568
GTACATG45250.028.4829641
CATGGGA22300.027.8936714
TACATGG45900.027.8277932
TGCGCGG2050.027.81806612-13
ACGCAGA27900.027.400657
ACATGGG46000.027.2511793
TATGCGC1056.920426E-527.133214
GCCCATA13000.026.7063121
Produced by FastQC (version 0.11.5)