##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224490_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844047
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18409401372198	34.0	31.0	34.0	31.0	34.0
2	32.35466745335272	34.0	31.0	34.0	31.0	34.0
3	32.49337536890718	34.0	31.0	34.0	31.0	34.0
4	35.79043347112187	37.0	37.0	37.0	35.0	37.0
5	35.806812890751345	37.0	37.0	37.0	35.0	37.0
6	35.90922661889682	37.0	37.0	37.0	35.0	37.0
7	35.87040769056699	37.0	37.0	37.0	35.0	37.0
8	35.90430390724687	37.0	37.0	37.0	35.0	37.0
9	37.629538402482325	39.0	39.0	39.0	35.0	39.0
10-11	37.698865702976256	39.0	39.0	39.0	35.0	39.0
12-13	37.68643393081191	39.0	39.0	39.0	35.0	39.0
14-15	39.165816003137266	41.0	40.0	41.0	36.0	41.0
16-17	39.00393461501552	41.0	39.5	41.0	36.0	41.0
18-19	39.08969168778516	41.0	40.0	41.0	36.0	41.0
20-21	39.14847277462037	41.0	40.0	41.0	36.0	41.0
22-23	39.10472521079987	41.0	40.0	41.0	36.0	41.0
24-25	39.0409082669567	41.0	39.5	41.0	35.5	41.0
26-27	38.86524447098326	41.0	39.0	41.0	35.0	41.0
28-29	38.78633595048617	41.0	39.0	41.0	35.0	41.0
30-31	38.70546367678577	41.0	39.0	41.0	35.0	41.0
32-33	38.50387893091262	40.5	39.0	41.0	35.0	41.0
34-35	38.4911882869082	40.0	38.0	41.0	35.0	41.0
36-37	38.478289123709935	40.0	38.0	41.0	35.0	41.0
38-39	38.349271426828125	40.0	38.0	41.0	34.0	41.0
40-41	38.23520609634298	40.0	38.0	41.0	34.0	41.0
42-43	38.063732825304754	40.0	38.0	41.0	33.5	41.0
44-45	37.85811631342804	40.0	37.5	41.0	33.0	41.0
46-47	37.653591565398614	40.0	37.0	41.0	33.0	41.0
48-49	37.41198535152663	40.0	36.5	41.0	32.0	41.0
50-51	36.104758976692054	38.5	35.0	40.0	30.0	40.5
52-53	36.41417480306191	39.0	35.0	40.0	30.5	40.5
54-55	36.84491799627272	39.0	35.0	41.0	31.0	41.0
56-57	36.6715852316281	39.0	35.0	41.0	31.0	41.0
58-59	36.50888042964432	39.0	35.0	41.0	31.0	41.0
60-61	36.25248890168439	38.0	35.0	41.0	31.0	41.0
62-63	35.97681467975124	37.0	35.0	41.0	30.5	41.0
64-65	35.74259075620196	37.0	35.0	40.0	30.0	41.0
66-67	35.51872881486457	36.0	35.0	40.0	30.0	41.0
68-69	35.23429737917438	36.0	35.0	40.0	29.0	41.0
70-71	34.963195769903805	35.0	35.0	39.0	29.0	41.0
72-73	34.6621207112874	35.0	34.0	39.0	29.0	41.0
74-75	34.39985628762379	35.0	34.0	38.5	29.0	40.5
76-77	34.08004234361357	35.0	34.0	37.0	28.0	39.5
78-79	33.85880229418504	35.0	34.0	37.0	28.5	39.0
80-81	33.56378850940765	35.0	34.0	36.5	28.0	39.0
82-83	33.30884358335496	35.0	34.0	36.0	27.0	37.5
84-85	33.07334544166379	35.0	34.0	36.0	27.0	37.0
86-87	32.849297491727356	35.0	33.5	35.0	27.0	37.0
88-89	32.692868406617166	35.0	33.0	35.0	27.0	36.0
90-91	32.5139737479074	35.0	33.0	35.0	26.0	36.0
92-93	32.3809764148205	35.0	33.0	35.0	25.5	36.0
94-95	32.238859328923624	35.0	33.0	35.0	25.0	35.5
96-97	32.10351615490606	35.0	33.0	35.0	25.0	35.0
98-99	32.05678475250786	35.0	33.0	35.0	25.0	35.0
100-101	30.817132813694023	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	87.0
8	321.0
9	303.0
10	436.0
11	886.0
12	1429.0
13	1723.0
14	1816.0
15	1866.0
16	2009.0
17	2159.0
18	2479.0
19	2851.0
20	3444.0
21	4546.0
22	5461.0
23	5022.0
24	4970.0
25	4953.0
26	5043.0
27	5314.0
28	5949.0
29	6946.0
30	8248.0
31	10456.0
32	13942.0
33	20187.0
34	33221.0
35	60076.0
36	128768.0
37	201785.0
38	223874.0
39	73462.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01330172527328	19.591776683835157	14.05870508991805	23.336216500973514
2	15.911355437210101	19.890408686740635	38.87280891421666	25.325426961832605
3	19.899104666468414	26.910714264557612	28.285881169561268	24.904299899412706
4	12.818125057016966	16.839939008135804	39.18703579303048	31.15490014181675
5	13.513465482372428	39.68013629572761	33.594930140146225	13.211468081753742
6	29.04767151592269	37.366639535476104	19.69475633465909	13.890932613942114
7	26.730501974416114	31.61127283196315	22.324941620549566	19.333283573071167
8	26.090371744701418	34.21195739099837	20.590678007267368	19.106992857032843
9	26.934045142035927	15.451746170533157	20.436421194554335	37.17778749287658
10-11	26.088713069295906	25.28668427232133	27.32513710729379	21.299465551088982
12-13	26.469912220528002	22.90500410522163	27.714214966702087	22.910868707548275
14-15	23.631798339964703	24.64048964285418	25.12637531304928	26.601336704131835
16-17	23.256537761083603	27.47015679244209	26.167584371976556	23.10572107449775
18-19	23.084553176965812	26.984892584235574	27.04076916513396	22.88978507366465
20-21	24.88858466972466	24.978724412995295	26.55874788133082	23.573943035949224
22-23	24.527861212329228	26.31688768672	25.846512435749467	23.3087386652013
24-25	23.520388484119184	25.921811409114042	26.544222596281173	24.013577510485597
26-27	23.387441694597573	26.8344653792976	26.822499221014944	22.955593705089882
28-29	23.060682639710823	27.106606622616987	26.85265157035094	22.98005916732125
30-31	23.372869046391965	25.973375890205162	27.017215865941115	23.636539197461754
32-33	23.259249781114086	26.086995155483052	27.159684235593517	23.494070827809352
34-35	24.016375865325035	25.699812925109622	26.604324166782185	23.679487042783162
36-37	22.941454954615992	25.90985768281143	27.423447999625527	23.725239362947047
38-39	23.122839901848458	26.20992155222565	26.54830977688415	24.11892876904174
40-41	24.007279672604305	25.586793271042215	26.99497863728889	23.41094841906459
42-43	23.832165197388413	26.125151583189034	26.98497704703211	23.057706172390443
44-45	23.944789325193646	25.750564915852124	26.385332450164768	23.919313308789462
46-47	23.643884759971897	25.733697294108033	26.282718853333996	24.339699092586077
48-49	23.223469783080798	25.934219302953508	26.811066208398348	24.031244705567346
50-51	23.282885905642694	26.342253452710573	26.384490437143903	23.99037020450283
52-53	23.64228532297372	26.335144843829788	25.613206373578723	24.40936345961777
54-55	23.732268463722992	25.53347147729925	26.13219406028337	24.602065998694385
56-57	23.612990287259226	26.220431113944027	26.040997765524626	24.12558083327212
58-59	24.07896210266993	26.31122107694231	26.30843638186346	23.3013804385243
60-61	23.797490554176694	26.43485566568688	25.91944580553306	23.84820797460337
62-63	23.412200839557322	25.98301923017666	26.485995131420825	24.118784798845194
64-65	24.449081773321936	25.2769259764567	25.861419070050268	24.412573180171094
66-67	23.281843696731155	25.916542588709568	25.725641270234384	25.07597244432489
68-69	23.467413544506407	26.118095319336483	26.8987982896687	23.515692846488406
70-71	23.941616995262113	25.63802726625413	26.48039741862716	23.939958319856597
72-73	23.787834089807795	25.892219272149536	26.59917042534361	23.720776212699054
74-75	23.77106962053061	25.6142134265035	26.55213512991575	24.062581823050138
76-77	23.565512347061244	25.226735004093374	25.921542283782774	25.28621036506261
78-79	23.640685610277636	24.80967613689217	26.678816742219517	24.87082151061068
80-81	23.27331634933299	25.983920126988792	26.20803543167536	24.534728092002858
82-83	23.394617212828127	26.25599355156084	26.36824854939753	23.981140686213497
84-85	23.393399977482414	25.134424053520743	27.07668422643209	24.395491742564754
86-87	23.36545597234505	26.468517126673607	26.211860046613193	23.95416685436815
88-89	23.69914074074074	25.588385185185185	25.92705185185185	24.785422222222223
90-91	23.202617863697164	25.49686214156321	26.30718431556536	24.993335679174265
92-93	23.066287050761186	25.61287708335555	26.78697315556314	24.533862710320125
94-95	23.668824129461985	25.336622249708846	26.621029397652023	24.373524223177146
96-97	23.82965640538975	26.00880045779441	26.565108341123185	23.596434795692655
98-99	22.761835931946354	26.158179707122887	26.593941045448084	24.48604331548268
100-101	23.604614017838042	25.44821794436414	26.100564632224515	24.846603405573305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	2.0
3	3.5
4	5.5
5	5.0
6	7.5
7	15.5
8	27.0
9	32.5
10	33.0
11	44.5
12	55.5
13	59.0
14	69.0
15	81.0
16	83.5
17	93.0
18	106.5
19	128.0
20	155.0
21	169.5
22	191.0
23	244.5
24	334.5
25	451.5
26	624.0
27	827.0
28	1068.5
29	1463.5
30	2135.5
31	3629.0
32	6356.5
33	10504.0
34	16077.0
35	21756.5
36	26228.5
37	28503.0
38	28742.0
39	27649.0
40	25863.0
41	23975.5
42	22569.0
43	22183.0
44	22773.5
45	24897.5
46	27705.5
47	28321.0
48	29398.5
49	33117.5
50	36814.0
51	36990.0
52	36066.5
53	36828.5
54	38667.0
55	39191.0
56	32712.0
57	26773.5
58	24868.0
59	23349.5
60	19797.5
61	14829.0
62	11893.5
63	8466.5
64	5090.5
65	3299.0
66	2661.0
67	2440.5
68	1826.0
69	1033.5
70	542.0
71	313.5
72	200.0
73	143.5
74	121.5
75	90.5
76	53.5
77	38.0
78	25.0
79	21.0
80	20.5
81	15.0
82	11.0
83	9.0
84	9.0
85	9.0
86	9.5
87	10.0
88	6.5
89	1.5
90	1.5
91	3.0
92	4.5
93	5.5
94	3.0
95	3.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1450156211680155
2	2.3695362935950248E-4
3	4.7390725871900496E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03909734884431791
16-17	0.07570668458036105
18-19	0.026420329673584528
20-21	0.042296222840671194
22-23	0.06770949958947783
24-25	0.017949237423982314
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022806786825852115
38-39	0.005449933475268558
40-41	0.006279271178026817
42-43	0.004561357365170423
44-45	0.013743310502851144
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.1847681467975124E-4
58-59	0.01759380697994306
60-61	0.018423144682701318
62-63	0.031870263148853085
64-65	0.04857549401869801
66-67	0.018423144682701318
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01854162149738107
80-81	0.060186221857313635
82-83	0.05118198394165254
84-85	0.031040925446094828
86-87	0.06030469867199338
88-89	0.03518761395988612
90-91	0.0
92-93	0.004265165328471045
94-95	0.0
96-97	0.0
98-99	8.293377027582588E-4
100-101	0.04744996427924038
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	844047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.479081790689406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31676876463136	33.612170102496734
2	15.057731298706742	13.997390492296288
3	5.474278419126892	7.633183031628141
4	2.5191466174007835	4.683504866916382
5	1.3389172820488604	3.1115822931658266
6	0.7974065158194654	2.2237633601520956
7	0.5198165258795363	1.6912416375754888
8	0.34777945970384705	1.29315759621575
9	0.2556029526127383	1.0692171486386253
>10	1.1349077935842113	9.607084781958077
>50	0.10633524726372204	3.478080080436303
>100	0.11637592881725999	11.363784025517798
>500	0.010556223630804515	3.21957924821236
>1k	0.004376970773748213	3.016261334790088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2616	0.30993534720222926	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2589	0.306736473205876	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2408	0.285292169748841	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2021	0.2394416424677773	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1627	0.19276177748395526	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1477	0.17499025528199258	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1296	0.15354595182495762	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1273	0.15082098508732333	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1215	0.14394932983589778	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1168	0.13838091954594944	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1156	0.13695919776979246	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1140	0.1350635687349164	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1132	0.13411575421747843	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1033	0.12238654956418303	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1029	0.12191264230546404	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1021	0.12096482778802603	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1004	0.11895072193847026	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	953	0.11290840438980293	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	908	0.10757694772921413	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	856	0.10141615336586707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	1.1847681467975124E-4	0.0	0.0	5.923840733987562E-5	0.0
18-19	1.1847681467975124E-4	0.0	0.0	1.1847681467975124E-4	0.0
20-21	1.1847681467975124E-4	0.0	0.0	2.3695362935950248E-4	0.0
22-23	1.7771522201962686E-4	0.0	0.0	7.700992954183831E-4	0.0
24-25	3.554304440392537E-4	0.0	0.0	0.0013624833688171394	0.0
26-27	3.554304440392537E-4	1.1847681467975124E-4	0.0	0.0019548674422158957	0.0
28-29	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.002606489922954527	0.0
30-31	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.005449933475268558	0.0
32-33	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.014098740946890398	0.0
34-35	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.024524700638708506	0.0
36-37	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.041289169915893306	0.0
38-39	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.0736333403234654	0.0
40-41	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.1139746957219207	0.0
42-43	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.14934002490382645	0.0
44-45	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.19625684351700795	0.0
46-47	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.25040074782565425	0.0
48-49	4.7390725871900496E-4	1.1847681467975124E-4	0.0	0.306736473205876	0.0
50-51	5.331456660588806E-4	1.1847681467975124E-4	0.0	0.36585640373107187	0.0
52-53	5.923840733987562E-4	1.1847681467975124E-4	0.0	0.42361385088745057	0.0
54-55	6.516224807386318E-4	1.1847681467975124E-4	0.0	0.4869989467411175	0.0
56-57	7.700992954183831E-4	1.1847681467975124E-4	0.0	0.5656083132811325	0.0
58-59	8.293377027582587E-4	1.1847681467975124E-4	0.0	0.653458871366168	0.0
60-61	8.293377027582587E-4	1.1847681467975124E-4	0.0	0.7484772767393284	0.0
62-63	8.885761100981342E-4	1.1847681467975124E-4	0.0	0.8567650853566211	0.0
64-65	9.478145174380099E-4	1.1847681467975124E-4	0.0	0.9645197483078549	0.0
66-67	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.0846552383931227	0.0
68-69	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.2309741045226155	0.0
70-71	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.3846977715695927	0.0
72-73	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.5554820999304542	0.0
74-75	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.7211719252600863	0.0
76-77	9.478145174380099E-4	1.1847681467975124E-4	0.0	1.898887147279713	0.0
78-79	9.478145174380099E-4	1.1847681467975124E-4	0.0	2.1039705134903626	0.0
80-81	9.478145174380099E-4	1.1847681467975124E-4	0.0	2.3320976201562233	0.0
82-83	9.478145174380099E-4	1.1847681467975124E-4	0.0	2.5883037319011857	0.0
84-85	9.478145174380099E-4	1.1847681467975124E-4	0.0	2.8497820618993965	0.0
86-87	9.478145174380099E-4	1.1847681467975124E-4	0.0	3.120975490701347	0.0
88-89	9.478145174380099E-4	1.1847681467975124E-4	0.0	3.429785308164119	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	210	0.0	54.298584	9
AAACCGT	225	0.0	52.759014	6
AACCGTG	235	0.0	52.53451	7
ACCGTGC	270	0.0	51.003403	8
GGTATCA	915	0.0	47.819073	1
GTATCAA	1675	0.0	45.713608	1
TTGTACG	80	1.0798969E-5	35.612335	4
ATCAACG	2235	0.0	34.204838	3
TCAACGC	2385	0.0	32.053593	4
GTCTAAT	505	0.0	30.136515	1
TATCAAC	2610	0.0	29.108423	2
CGCAGAG	2665	0.0	28.68756	8
GTACATG	4525	0.0	28.482964	1
CATGGGA	2230	0.0	27.893671	4
TACATGG	4590	0.0	27.827793	2
TGCGCGG	205	0.0	27.818066	12-13
ACGCAGA	2790	0.0	27.40065	7
ACATGGG	4600	0.0	27.251179	3
TATGCGC	105	6.920426E-5	27.13321	4
GCCCATA	1300	0.0	26.706312	1
>>END_MODULE