FastQCFastQC Report
Fri 10 Feb 2017
SRR3224490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844047
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG16660.19738237325646557No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA16600.1966715123683871No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT16350.1937095920013933No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG14270.16906641454800503No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA13620.1613654215938212No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13620.1613654215938212No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG13330.1579295939681084No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13090.15508615041579438No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG12880.1525981373075196No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG12660.14999164738456508No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG11890.14086893265422423No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA11640.13790701228723046No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT11570.1370776745844722No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT10940.12961363525964786No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT10930.12949515844496812No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA10710.12688866852201358No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG10600.1255854235605363No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10310.12214959593482354No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA9920.11752900016231324No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC9840.11658118564487523No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT9680.1146855566099992No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC9640.11421164935128021No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8690.10295635195670383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.051.419281
GTATCAA19300.045.5199551
GCGTAAT751.307908E-744.5633741
AAACCGT2350.044.457856
CCGTGCG2550.040.9709639
ACCGTGC2550.040.9709638
AACCGTG2600.040.183067
CGTAATT951.931221E-839.9907952
ATCAACG25450.034.5205423
TCAACGC26550.033.0903134
TCAGCGC1401.7085767E-830.5286878
CAACGCA30150.029.2967385
TATCAAC30350.028.9472082
AACGCAG31450.028.2367446
CGCAGAG31950.027.4975818
GTACATG52000.027.2705821
AATGCGG1955.366019E-1026.7887067
TACATGG52000.026.7582652
TACTCCG1257.4010713E-626.593887
ACGCAGA33450.026.264517
Produced by FastQC (version 0.11.5)