##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844047
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.17079380650603	34.0	33.0	34.0	31.0	34.0
2	33.2934635156573	34.0	33.0	34.0	31.0	34.0
3	33.412376325015074	34.0	34.0	34.0	31.0	34.0
4	36.62362522466166	37.0	37.0	37.0	35.0	37.0
5	36.66107337624564	37.0	37.0	37.0	35.0	37.0
6	36.70511831687098	37.0	37.0	37.0	36.0	37.0
7	36.68392755379736	37.0	37.0	37.0	36.0	37.0
8	36.67249928025335	37.0	37.0	37.0	36.0	37.0
9	38.51913696749115	39.0	39.0	39.0	37.0	39.0
10-11	38.545169877980726	39.0	39.0	39.0	37.5	39.0
12-13	38.5335816607369	39.0	39.0	39.0	37.0	39.0
14-15	40.135907123655436	41.0	40.0	41.0	38.0	41.0
16-17	40.14667962803019	41.0	40.0	41.0	38.0	41.0
18-19	40.140705434649966	41.0	40.0	41.0	38.0	41.0
20-21	40.1432923758985	41.0	40.0	41.0	38.0	41.0
22-23	40.07193082849652	41.0	40.0	41.0	38.0	41.0
24-25	40.03407985574263	41.0	40.0	41.0	38.0	41.0
26-27	39.969942432115744	41.0	40.0	41.0	38.0	41.0
28-29	39.94246706640744	41.0	40.0	41.0	38.0	41.0
30-31	39.87402597248732	41.0	40.0	41.0	38.0	41.0
32-33	39.82256675279931	41.0	40.0	41.0	38.0	41.0
34-35	39.73384420535823	41.0	40.0	41.0	37.0	41.0
36-37	39.612941577897914	41.0	40.0	41.0	37.0	41.0
38-39	39.49524197112246	41.0	40.0	41.0	36.0	41.0
40-41	39.35361360208614	41.0	39.0	41.0	36.0	41.0
42-43	39.24093563510089	41.0	39.0	41.0	35.0	41.0
44-45	38.973973605735225	41.0	39.0	41.0	35.0	41.0
46-47	38.73316533321012	40.5	38.0	41.0	35.0	41.0
48-49	38.60304284003142	40.0	38.0	41.0	35.0	41.0
50-51	38.407717224277796	40.0	37.0	41.0	35.0	41.0
52-53	38.13426503500398	40.0	37.0	41.0	35.0	41.0
54-55	37.932815945083625	40.0	36.0	41.0	34.5	41.0
56-57	37.68454777992221	39.0	35.5	41.0	34.0	41.0
58-59	37.402708616937204	39.0	35.0	41.0	34.0	41.0
60-61	37.153226064425326	39.0	35.0	41.0	34.0	41.0
62-63	36.949394998145834	38.0	35.0	41.0	33.0	41.0
64-65	36.6999959717883	37.0	35.0	41.0	33.0	41.0
66-67	36.445438464919604	37.0	35.0	40.0	33.0	41.0
68-69	36.19564135646475	36.0	35.0	40.0	33.0	41.0
70-71	35.92813907282414	36.0	35.0	39.0	33.0	41.0
72-73	35.644187468233405	35.0	35.0	39.0	32.5	41.0
74-75	35.34756832261711	35.0	35.0	39.0	32.0	40.5
76-77	33.52401110364707	34.5	32.5	36.5	29.0	39.0
78-79	34.8150043777183	35.0	34.5	37.0	32.0	39.0
80-81	34.73236383755881	35.0	35.0	37.0	33.0	39.0
82-83	34.52759206537077	35.0	35.0	36.0	32.0	38.0
84-85	34.31745033155737	35.0	35.0	36.0	32.0	37.0
86-87	34.120225532464424	35.0	35.0	35.5	32.0	37.0
88-89	33.97071430856339	35.0	35.0	35.0	32.0	36.5
90-91	33.823059023964305	35.0	34.5	35.0	32.0	36.0
92-93	33.72586005281696	35.0	34.5	35.0	31.5	36.0
94-95	33.62553447853023	35.0	34.0	35.0	31.0	36.0
96-97	33.5792130059108	35.0	34.0	35.0	31.0	35.5
98-99	33.5497022085263	35.0	34.0	35.0	31.0	35.0
100-101	32.709903003031826	34.5	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	11.0
15	48.0
16	126.0
17	272.0
18	483.0
19	752.0
20	1144.0
21	1806.0
22	2502.0
23	2358.0
24	2124.0
25	2384.0
26	3103.0
27	4128.0
28	5242.0
29	5962.0
30	6403.0
31	6936.0
32	8381.0
33	11256.0
34	19000.0
35	40599.0
36	117821.0
37	235200.0
38	265061.0
39	100924.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80473508723688	19.394121197187374	13.908857486706449	22.892286228869295
2	15.78371820526582	20.015946979255894	39.459650943608594	24.740683871869695
3	19.650327529154183	27.350017238376534	28.447823403199113	24.55183182927017
4	12.95472882434272	17.019075951931587	39.509766636218124	30.516428587507566
5	13.42188290462498	38.763955087809094	34.356736058537024	13.457425949028906
6	28.959287812171596	36.83752208111633	20.211078293033445	13.992111813678623
7	26.859049318343647	31.459385555543705	22.53203909260977	19.149526033502873
8	26.299957229869904	33.69409523403318	20.45075688913058	19.55519064696634
9	27.222773139410485	15.743436088274704	20.47480768251057	36.558983089804244
10-11	26.264769615910016	25.223476891689682	27.498587163984944	21.013166328415362
12-13	26.822499221014944	23.20907485009721	27.58495676188648	22.383469167001362
14-15	23.880246005258	25.00032581124037	25.29438526527551	25.825042918226117
16-17	23.14871091301788	27.631636626870304	26.297824647205665	22.921827812906155
18-19	23.011988668877446	26.82007044631401	27.66943073075315	22.498510154055403
20-21	24.763266691586484	25.405297494610746	26.961383962025522	22.870051851777255
22-23	24.53562517327419	26.245323077870903	26.297453432191443	22.92159831666347
24-25	23.97937785233945	25.668330087057072	26.606136646904666	23.746155413698812
26-27	23.62180468810628	26.855163237777823	26.79722687417916	22.725805199936733
28-29	23.27654946156425	26.8691226145383	27.224505609470878	22.62982231442657
30-31	23.560261219228426	25.737700256622304	27.283357937164265	23.41868058698501
32-33	23.44550922253691	26.175275888236015	27.382614583438237	22.99660030578884
34-35	24.160804258256	25.85759012804981	26.60088921010773	23.38071640358646
36-37	22.933786870639974	25.89362725583864	27.45316528283288	23.719420590688507
38-39	23.311912725239235	26.12650717317874	26.758758694717237	23.802821406864783
40-41	23.716688762592604	26.02088509289175	27.044406294910118	23.218019849605533
42-43	23.72747015272846	26.18550862688926	27.137647548063082	22.949373672319197
44-45	24.01358351778038	25.721298538800237	26.78988677242157	23.475231170997816
46-47	23.607498919553734	25.66150285480877	26.529298323514954	24.201699902122538
48-49	23.286514857115776	25.596357992512203	27.069214729159757	24.047912421212263
50-51	23.70371555138517	25.816275633939817	26.7095315782178	23.77047723645721
52-53	23.77379458726824	26.244628557414458	26.055361845963553	23.926215009353744
54-55	23.90500765952607	25.475358599698833	26.560902414202054	24.058731326573046
56-57	24.009531014601624	25.910994494547566	26.123262180509883	23.956212310340927
58-59	24.338919086777906	25.935173178901355	26.419057146311342	23.306850588009397
60-61	23.815325394417474	26.296256352396846	26.214007035042474	23.674411218143206
62-63	23.53156873965549	25.76313878255595	26.771257998665952	23.934034479122605
64-65	24.112579038844995	25.52103141175788	26.208196936900436	24.1581926124967
66-67	23.60490588794226	25.843169870872117	26.17881468685985	24.373109554325765
68-69	24.022714374910404	25.71397090446385	26.707102803516864	23.556211917108882
70-71	24.213106616100763	25.758636663598118	26.487446789100606	23.540809931200513
72-73	23.815794618072218	25.682396833351696	26.703548499076472	23.798260049499614
74-75	23.87136024415702	25.556219025717763	26.42032967358453	24.15209105654069
76-77	23.761484139758036	24.991395595848324	26.38101427116698	24.86610599322666
78-79	23.867771278127247	24.880417357345046	26.408717299168107	24.8430940653596
80-81	23.15177344795534	25.91542687337491	26.424910677417962	24.50788900125179
82-83	23.354266422680954	25.981457006996717	26.769454236980895	23.894822333341427
84-85	23.6331474588832	25.26046217633646	26.96775049657683	24.1386398682035
86-87	23.247757530090148	26.20061442076093	26.47636920692805	24.07525884222087
88-89	23.84061551074762	25.327973442237223	25.91828417137908	24.91312687563607
90-91	23.660353037212385	25.57659703784268	25.978766585273092	24.78428333967184
92-93	23.593354398510982	25.59448703685932	26.58193204880771	24.230226515821986
94-95	23.644773336081997	25.374297876777003	26.355048948695988	24.625879838445016
96-97	23.822133127657583	26.061700355548922	26.365001001129084	23.751165515664415
98-99	22.389748438179392	26.303274580680934	26.7677629326329	24.53921404850678
100-101	23.320037439529763	25.7442410640983	26.19658598436147	24.739135512010467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	3.0
2	2.5
3	2.5
4	1.0
5	2.5
6	7.5
7	19.0
8	29.5
9	39.5
10	43.5
11	49.5
12	56.5
13	59.0
14	58.0
15	63.0
16	84.5
17	89.5
18	102.0
19	122.5
20	147.5
21	175.0
22	212.5
23	266.5
24	315.5
25	425.0
26	641.0
27	858.5
28	1061.5
29	1463.0
30	2212.5
31	3753.5
32	6439.0
33	10565.0
34	15858.5
35	21443.0
36	26122.5
37	28398.0
38	28764.5
39	27592.5
40	25714.5
41	24031.0
42	22827.5
43	22923.5
44	23893.0
45	25713.5
46	27662.0
47	28798.0
48	29809.5
49	33482.5
50	38466.5
51	38293.5
52	35528.0
53	35732.5
54	38049.5
55	38144.0
56	32224.5
57	26732.5
58	25197.0
59	24254.5
60	20094.5
61	14572.5
62	11535.0
63	7960.5
64	4506.0
65	2783.0
66	2285.0
67	2000.0
68	1420.5
69	814.0
70	373.0
71	207.0
72	139.0
73	97.0
74	82.5
75	62.0
76	34.5
77	20.5
78	11.0
79	4.0
80	3.0
81	4.0
82	3.5
83	1.5
84	0.5
85	1.5
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47130076879605043
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.700992954183831E-4
22-23	0.0010662913321177613
24-25	3.554304440392538E-4
26-27	0.0020733442568956467
28-29	0.003317350811033035
30-31	5.923840733987563E-4
32-33	5.923840733987562E-5
34-35	0.004679834179850174
36-37	0.0017771522201962687
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009833575618419353
46-47	0.07647678387577943
48-49	8.293377027582588E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007760231361523707
58-59	0.041822315581952194
60-61	0.032107216778212586
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.9619203669937815E-4
78-79	0.008293377027582587
80-81	0.0065754632147261936
82-83	0.0010070529247778856
84-85	0.002191821071575398
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06557691692524231
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	844047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.45250153220753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48055010131198	31.330367613693998
2	15.921193175543275	14.15473728060819
3	5.850112791145605	7.801564434359605
4	2.65343856030451	4.7180792667021905
5	1.495770935221771	3.3245379894888627
6	0.876079438319383	2.336635354453673
7	0.5919195052599114	1.8418611900156816
8	0.38399983567354273	1.3655802626916478
9	0.2703134332664857	1.081449747580926
>10	1.2118399083723173	9.734992703755088
>50	0.12132553586046446	3.8370076800039414
>100	0.12745788235464198	12.384917306452069
>500	0.01119922815625516	3.34224214998763
>1k	0.0047996692098236406	2.746027020206526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1666	0.19738237325646557	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1660	0.1966715123683871	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1635	0.1937095920013933	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1427	0.16906641454800503	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1362	0.1613654215938212	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1362	0.1613654215938212	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1333	0.1579295939681084	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1309	0.15508615041579438	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1288	0.1525981373075196	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1266	0.14999164738456508	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1189	0.14086893265422423	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1164	0.13790701228723046	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1157	0.1370776745844722	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1094	0.12961363525964786	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1093	0.12949515844496812	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1071	0.12688866852201358	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1060	0.1255854235605363	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1031	0.12214959593482354	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	992	0.11752900016231324	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	984	0.11658118564487523	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	968	0.1146855566099992	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	964	0.11421164935128021	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	869	0.10295635195670383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	5.923840733987562E-5	0.0
18-19	0.0	0.0	0.0	1.1847681467975124E-4	0.0
20-21	0.0	0.0	0.0	3.554304440392537E-4	0.0
22-23	1.7771522201962686E-4	0.0	0.0	8.293377027582587E-4	0.0
24-25	3.554304440392537E-4	0.0	0.0	0.0013624833688171394	0.0
26-27	3.554304440392537E-4	0.0	0.0	0.0020733442568956467	0.0
28-29	3.554304440392537E-4	0.0	0.0	0.002724966737634279	0.0
30-31	3.554304440392537E-4	0.0	0.0	0.005449933475268558	0.0
32-33	3.554304440392537E-4	0.0	0.0	0.014217217761570148	0.0
34-35	3.554304440392537E-4	0.0	0.0	0.02464317745338826	0.0
36-37	3.554304440392537E-4	0.0	0.0	0.041585361952592687	0.0
38-39	3.554304440392537E-4	0.0	0.0	0.0741072475821844	0.0
40-41	3.554304440392537E-4	0.0	0.0	0.11433012616595994	0.0
42-43	3.554304440392537E-4	0.0	0.0	0.1498731705698853	0.0
44-45	3.554304440392537E-4	0.0	0.0	0.19702694281242633	0.0
46-47	3.554304440392537E-4	0.0	0.0	0.25188170800915116	0.0
48-49	3.554304440392537E-4	0.0	0.0	0.3086913406480919	0.0
50-51	4.1466885137912934E-4	0.0	0.0	0.3689960393200853	0.0
52-53	5.331456660588806E-4	0.0	0.0	0.4275828241792222	0.0
54-55	5.923840733987562E-4	0.0	0.0	0.49126411206958853	0.0
56-57	5.923840733987562E-4	0.0	0.0	0.5710582467564009	0.0
58-59	5.923840733987562E-4	0.0	0.0	0.6604490034322733	0.0
60-61	5.923840733987562E-4	0.0	0.0	0.7567706537669111	0.0
62-63	5.923840733987562E-4	0.0	0.0	0.8660655153089816	0.0
64-65	5.923840733987562E-4	0.0	0.0	0.9757158072950914	0.0
66-67	5.923840733987562E-4	0.0	0.0	1.0981615952666144	0.0
68-69	5.923840733987562E-4	0.0	0.0	1.2466130440603425	0.0
70-71	5.923840733987562E-4	0.0	0.0	1.4024100553642156	0.0
72-73	5.923840733987562E-4	0.0	0.0	1.5768079265728092	0.0
74-75	5.923840733987562E-4	0.0	0.0	1.7452227186400757	0.0
76-77	5.923840733987562E-4	0.0	0.0	1.927025390766154	0.0
78-79	5.923840733987562E-4	0.0	0.0	2.1344190548630584	0.0
80-81	5.923840733987562E-4	0.0	0.0	2.3649749362298547	0.0
82-83	5.923840733987562E-4	0.0	0.0	2.6244983987858497	0.0
84-85	5.923840733987562E-4	0.0	0.0	2.8913081854446494	0.0
86-87	5.923840733987562E-4	0.0	0.0	3.16724068683379	0.0
88-89	5.923840733987562E-4	0.0	0.0	3.4789531862562155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	975	0.0	51.41928	1
GTATCAA	1930	0.0	45.519955	1
GCGTAAT	75	1.307908E-7	44.563374	1
AAACCGT	235	0.0	44.45785	6
CCGTGCG	255	0.0	40.970963	9
ACCGTGC	255	0.0	40.970963	8
AACCGTG	260	0.0	40.18306	7
CGTAATT	95	1.931221E-8	39.990795	2
ATCAACG	2545	0.0	34.520542	3
TCAACGC	2655	0.0	33.090313	4
TCAGCGC	140	1.7085767E-8	30.528687	8
CAACGCA	3015	0.0	29.296738	5
TATCAAC	3035	0.0	28.947208	2
AACGCAG	3145	0.0	28.236744	6
CGCAGAG	3195	0.0	27.497581	8
GTACATG	5200	0.0	27.270582	1
AATGCGG	195	5.366019E-10	26.788706	7
TACATGG	5200	0.0	26.758265	2
TACTCCG	125	7.4010713E-6	26.59388	7
ACGCAGA	3345	0.0	26.26451	7
>>END_MODULE