FastQCFastQC Report
Fri 10 Feb 2017
SRR3224489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27267
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3153995672424542No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC820.3007298199288517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.2493857043312429No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA660.24205083067444164No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT490.17970440459163092No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA490.17970440459163092No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC340.12469285216562145No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG340.12469285216562145No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA340.12469285216562145No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA330.12102541533722082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCA156.549208E-494.996324
AGACCAG156.549208E-494.996325
GTAGACC200.002052661771.2472463
GGTATCA603.0013325E-1063.564151
GACCAGG250.00496972756.9977956
GCCTGAC250.00496972756.9977952
ACCAGGC250.00496972756.9977957
TGTAGAC250.00496972756.9977952
GTATCAA803.970854E-947.4981652
ATCAACG1106.596565E-834.544124
ACGCAGA1307.677954E-932.8833478
TATCAAC1351.1094016E-831.6654433
AGTACAT753.0015328E-431.6654432
TGGTCGC300.003919042631.66544282-83
TCAACGC1201.4168836E-731.6654425
GAGTACA804.2880047E-429.7956961
CGCAGAG1503.0935553E-828.4988989
TTCTCCC350.00832441227.14180868-69
CAACGCA1605.7889338E-826.7177186
AACGCAG1605.7889338E-826.7177187
Produced by FastQC (version 0.11.5)