##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224489_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27267
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936443319763818	34.0	31.0	34.0	30.0	34.0
2	32.07562254740162	34.0	31.0	34.0	30.0	34.0
3	32.18450874683683	34.0	31.0	34.0	30.0	34.0
4	35.46275717900759	37.0	35.0	37.0	35.0	37.0
5	35.46741482377966	37.0	35.0	37.0	35.0	37.0
6	35.59551839219569	37.0	37.0	37.0	35.0	37.0
7	35.54659478490483	37.0	37.0	37.0	35.0	37.0
8	35.57721788242198	37.0	37.0	37.0	35.0	37.0
9	37.3015366560311	39.0	38.0	39.0	35.0	39.0
10-11	37.39228738034987	39.0	39.0	39.0	35.0	39.0
12-13	37.36412146550776	39.0	39.0	39.0	35.0	39.0
14-15	38.80208677155536	41.0	39.0	41.0	36.0	41.0
16-17	38.61849488392562	41.0	39.0	41.0	35.5	41.0
18-19	38.733414016943556	41.0	39.5	41.0	35.0	41.0
20-21	38.80261854989548	41.0	40.0	41.0	35.0	41.0
22-23	38.742820992408404	41.0	39.5	41.0	35.0	41.0
24-25	38.673964865955185	41.0	39.0	41.0	35.0	41.0
26-27	38.515953350203546	41.0	39.0	41.0	34.5	41.0
28-29	38.40717717387318	41.0	39.0	41.0	34.0	41.0
30-31	38.24183078446474	40.5	38.5	41.0	34.0	41.0
32-33	37.977261891663915	40.0	38.0	41.0	33.0	41.0
34-35	37.95168151978582	40.0	38.0	41.0	33.0	41.0
36-37	37.88135841860124	40.0	38.0	41.0	33.0	41.0
38-39	37.70840209777387	40.0	38.0	41.0	33.0	41.0
40-41	37.52426009461987	40.0	37.5	41.0	32.0	41.0
42-43	37.29917115927678	40.0	37.0	41.0	31.0	41.0
44-45	37.04224887226317	40.0	36.5	41.0	30.5	41.0
46-47	36.71960611728463	40.0	36.0	41.0	29.5	41.0
48-49	36.38696959694869	40.0	35.5	41.0	28.0	41.0
50-51	35.02651556826933	38.0	34.0	40.0	25.5	40.5
52-53	35.327887189643164	38.5	35.0	40.0	26.0	40.5
54-55	35.73820002200462	39.0	35.0	41.0	27.0	41.0
56-57	35.54406425349323	38.5	35.0	41.0	26.0	41.0
58-59	35.35568636080244	37.5	35.0	41.0	26.0	41.0
60-61	35.10538379726409	37.0	35.0	41.0	26.0	41.0
62-63	34.822642754978546	36.5	35.0	40.0	26.0	41.0
64-65	34.575439175560206	36.0	34.5	40.0	26.0	41.0
66-67	34.32212931382257	35.5	34.0	39.5	25.0	41.0
68-69	34.01617339641325	35.0	34.0	39.0	24.5	41.0
70-71	33.7462683830271	35.0	34.0	39.0	24.0	41.0
72-73	33.43559980929328	35.0	34.0	37.5	23.5	40.5
74-75	33.189459786555176	35.0	34.0	37.0	23.0	40.0
76-77	32.871309641691425	35.0	33.5	37.0	23.0	39.0
78-79	32.66938056991968	35.0	33.0	36.0	23.0	39.0
80-81	32.37776433050941	35.0	33.0	36.0	21.0	38.5
82-83	32.18828620677009	35.0	33.0	35.5	21.0	37.0
84-85	31.988997689514797	35.0	33.0	35.0	20.0	37.0
86-87	31.791011112333592	35.0	33.0	35.0	20.0	36.5
88-89	31.6270400117358	35.0	33.0	35.0	20.0	36.0
90-91	31.48259801224924	35.0	33.0	35.0	20.0	36.0
92-93	31.355227931198883	35.0	32.5	35.0	20.0	36.0
94-95	31.22098140609528	35.0	32.0	35.0	19.5	35.0
96-97	31.09282282612682	35.0	32.0	35.0	19.0	35.0
98-99	31.037847948069093	35.0	32.0	35.0	19.0	35.0
100-101	29.80993508636814	34.0	29.5	35.0	14.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	10.0
8	16.0
9	24.0
10	29.0
11	84.0
12	119.0
13	127.0
14	109.0
15	82.0
16	109.0
17	107.0
18	119.0
19	181.0
20	208.0
21	253.0
22	331.0
23	293.0
24	228.0
25	163.0
26	200.0
27	202.0
28	217.0
29	237.0
30	276.0
31	321.0
32	477.0
33	621.0
34	981.0
35	1870.0
36	4106.0
37	6678.0
38	6498.0
39	1988.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.902306449243426	18.910680182165418	11.991332451887763	24.195680916703395
2	17.713719881175045	19.672131147540984	36.42498257967507	26.189166391608904
3	21.09876407378883	24.586496497597828	29.53753621593868	24.77720321267466
4	13.650199875307148	16.763853742619283	37.44453001797044	32.14141636410312
5	13.818901969413577	37.77826676935489	34.154839182895074	14.24799207833645
6	29.893277588293543	36.248945611911836	18.52789085707999	15.329885942714636
7	26.068140976271685	31.3969266879378	22.20632999596582	20.328602339824698
8	26.486228774709357	33.076612755345295	20.783364506546377	19.65379396339898
9	26.04613635530128	14.402024425129278	20.74669013826237	38.80514908130707
10-11	26.29735577804672	24.091392525763744	27.448930942164523	22.16232075402501
12-13	26.62925881101698	22.998496350900353	27.331572963655702	23.040671874426963
14-15	24.117183057252397	24.608808907966907	25.535193440096858	25.738814594683834
16-17	22.994200983630623	26.693826616751082	27.011304411656756	23.300667987961535
18-19	22.784299339691856	25.99046221570066	28.14930300807043	23.07593543653705
20-21	24.379953037863224	24.29189903140593	28.179116524801877	23.14903140592897
22-23	24.070539875949645	25.77898484236797	27.564502514038242	22.585972767644144
24-25	22.93202743846521	25.13847621143758	28.42338872381791	23.5061076262793
26-27	22.815124509480324	26.049803792129683	28.288773975868263	22.84629772252173
28-29	22.79311988850992	26.22584075989291	28.587670077382914	22.39336927421425
30-31	22.644588696959694	25.679392672461216	28.72703267686214	22.948985953716946
32-33	22.899475556533538	25.07976675101771	28.453808633146295	23.566949059302452
34-35	23.180034473906186	25.497854549455383	27.9238640114424	23.398246965196023
36-37	22.527694226395717	25.561220746827086	28.963392267625267	22.947692759151934
38-39	22.96576133800363	25.35531552018192	28.201507454748846	23.477415687065598
40-41	23.484862376909394	25.263601855757063	28.417655364641593	22.833880402691946
42-43	23.545376194231014	25.368125722039863	28.10041626171309	22.98608182201603
44-45	23.574611674521815	25.109574721707716	27.634836509013557	23.68097709475692
46-47	23.400080683610224	25.092602779917115	27.118861627608464	24.388454908864198
48-49	23.038838156012762	25.725235632816222	27.296732313785892	23.939193897385117
50-51	23.169032163420987	26.23317563354971	26.519235706164963	24.07855649686434
52-53	23.65680126159827	26.126819965526092	26.130487402354497	24.085891370521143
54-55	23.297392452415007	25.40983606557377	26.555910074448967	24.736861407562255
56-57	23.731983716580483	26.433050940697544	26.024131734330876	23.810833608391096
58-59	23.968306371739846	26.11056087450937	26.372840321338174	23.548292432412605
60-61	23.79223065918345	26.249954146949854	26.23344704889769	23.724368144969006
62-63	23.669246854249973	26.193183902564293	26.675593382002273	23.46197586118346
64-65	24.67251311782189	25.62470186768429	25.885223645103288	23.817561369390525
66-67	23.270913192597618	25.847807347358	25.897327733250187	24.983951726794196
68-69	23.456925954450433	25.914108629478854	27.120695346022668	23.50827007004804
70-71	23.54861187516045	25.32181758169216	26.731947042212195	24.397623500935197
72-73	24.00154032346793	25.22829794256794	26.66593317930099	24.104228554663145
74-75	23.47709685700664	25.954450434591266	26.605420471632375	23.96303223676972
76-77	24.12439945721935	25.318150144863754	25.63905086734881	24.918399530568085
78-79	23.732668182818575	25.045851368204826	26.11693932946959	25.104541119507008
80-81	23.356391860401107	26.134424484852932	26.12525000458724	24.383933650158717
82-83	23.443720759563345	25.99578020365104	26.476470048619394	24.084028988166224
84-85	23.188246299455237	25.62959702122196	26.94106641721235	24.241090262110458
86-87	23.244889720723698	26.384454475393593	26.448677015670302	23.92197878821241
88-89	23.492616710996973	25.745207740988718	26.002017793267907	24.760157754746402
90-91	23.220376279018595	25.87926797960905	26.205669857336705	24.694685884035646
92-93	23.13052407672278	25.798584369384237	26.731947042212195	24.338944511680786
94-95	23.642131514284667	25.5657021307808	26.78512487622401	24.007041478710526
96-97	23.396413246781826	25.963619026662265	27.095023288223864	23.54494443833205
98-99	22.62075035757509	27.018007114827448	26.642094839916382	23.71914768768108
100-101	23.449224841757637	26.23245573800569	26.082010824694983	24.236308595541693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.0
4	2.0
5	2.0
6	1.0
7	3.0
8	6.5
9	9.0
10	8.0
11	11.5
12	13.5
13	14.0
14	19.0
15	17.5
16	16.0
17	19.0
18	21.5
19	24.0
20	32.0
21	44.0
22	48.0
23	60.5
24	82.0
25	114.0
26	146.5
27	180.0
28	210.5
29	237.0
30	264.0
31	285.5
32	336.5
33	380.5
34	408.0
35	451.0
36	501.5
37	526.5
38	545.0
39	587.5
40	630.5
41	674.0
42	696.0
43	714.5
44	751.5
45	791.0
46	888.5
47	951.0
48	982.5
49	1091.0
50	1190.0
51	1175.5
52	1120.0
53	1177.5
54	1241.0
55	1234.5
56	1068.5
57	861.0
58	787.0
59	763.5
60	674.5
61	530.0
62	411.0
63	279.5
64	184.5
65	148.0
66	130.0
67	116.5
68	101.0
69	70.5
70	45.5
71	35.5
72	31.0
73	22.5
74	17.0
75	14.0
76	9.5
77	8.5
78	6.0
79	2.0
80	1.5
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	1.0
87	1.0
88	0.0
89	0.0
90	1.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1430300363076246
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03850808669820662
16-17	0.07701617339641324
18-19	0.025672057798804413
20-21	0.04034180511240694
22-23	0.07334873656801262
24-25	0.022004620970403784
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.018337184142003155
38-39	0.009168592071001578
40-41	0.0018337184142003153
42-43	0.0018337184142003153
44-45	0.009168592071001578
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.022004620970403784
60-61	0.022004620970403784
62-63	0.029339494627205046
64-65	0.051344115597608826
66-67	0.02017090255620347
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.018337184142003155
80-81	0.06418014449701104
82-83	0.053177834011809144
84-85	0.02750577621300473
86-87	0.06601386291121135
88-89	0.03484064986980599
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.053177834011809144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	27267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.21663549345362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87962962962963	71.99178494150438
2	5.782407407407407	9.161257197344776
3	1.0324074074074074	2.4535152382000223
4	0.5231481481481481	1.657681446437085
5	0.30092592592592593	1.191916969230205
6	0.2916666666666667	1.3862911211354385
7	0.24537037037037035	1.3606190633366342
8	0.17592592592592593	1.1149007958337918
9	0.1527777777777778	1.0892287380349874
>10	0.5972222222222222	7.485238566765687
>50	0.018518518518518517	1.1075659221769905
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.3153995672424542	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	82	0.3007298199288517	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.2493857043312429	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	66	0.24205083067444164	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	49	0.17970440459163092	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	49	0.17970440459163092	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	34	0.12469285216562145	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	34	0.12469285216562145	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	34	0.12469285216562145	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	33	0.12102541533722082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.02017090255620347	0.0
30-31	0.0	0.0	0.0	0.0476766787692082	0.0
32-33	0.0	0.0	0.0	0.0733487365680126	0.0
34-35	0.0	0.0	0.0	0.11369054168041956	0.0
36-37	0.0	0.0	0.0	0.16320093886382808	0.0
38-39	0.0	0.0	0.0	0.23288223860344004	0.0
40-41	0.0	0.0	0.0	0.27872519895844794	0.0
42-43	0.0	0.0	0.0	0.31539956724245427	0.0
44-45	0.0	0.0	0.0	0.3924157406388675	0.0
46-47	0.0	0.0	0.0	0.49510397183408517	0.0
48-49	0.0	0.0	0.0	0.5977922030293028	0.0
50-51	0.0	0.0	0.0	0.6839769684967176	0.0
52-53	0.0	0.0	0.0	0.7279862104375252	0.0
54-55	0.0	0.0	0.0	0.819672131147541	0.0
56-57	0.0	0.0	0.0	0.9223603623427586	0.0
58-59	0.0	0.0	0.0	1.0452194960941799	0.0
60-61	0.0	0.0	0.0	1.1735797850882017	0.0
62-63	0.0	0.0	0.0	1.331279568709429	0.0
64-65	0.0	0.0	0.0	1.459639857703451	0.0
66-67	0.0	0.0	0.0	1.6356768254666814	0.0
68-69	0.0	0.0	0.0	1.80254520115891	0.0
70-71	0.0	0.0	0.0	1.9455752374665347	0.0
72-73	0.0	0.0	0.0	2.110609894744563	0.0
74-75	0.0	0.0	0.0	2.295815454578795	0.0
76-77	0.0	0.0	0.0	2.517695382697033	0.0
78-79	0.0	0.0	0.0	2.728573000330069	0.0
80-81	0.0	0.0	0.0	3.0164667913595187	0.0
82-83	0.0	0.0	0.0	3.3208640481167713	0.0
84-85	0.0	0.0	0.0	3.5904206550042175	0.0
86-87	0.0	0.0	0.0	3.89481791176147	0.0
88-89	0.0	0.0	0.0	4.224887226317527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCA	15	6.549208E-4	94.99632	4
AGACCAG	15	6.549208E-4	94.99632	5
GTAGACC	20	0.0020526617	71.247246	3
GGTATCA	60	3.0013325E-10	63.56415	1
GACCAGG	25	0.004969727	56.997795	6
GCCTGAC	25	0.004969727	56.997795	2
ACCAGGC	25	0.004969727	56.997795	7
TGTAGAC	25	0.004969727	56.997795	2
GTATCAA	80	3.970854E-9	47.498165	2
ATCAACG	110	6.596565E-8	34.54412	4
ACGCAGA	130	7.677954E-9	32.883347	8
TATCAAC	135	1.1094016E-8	31.665443	3
AGTACAT	75	3.0015328E-4	31.665443	2
TGGTCGC	30	0.0039190426	31.665442	82-83
TCAACGC	120	1.4168836E-7	31.665442	5
GAGTACA	80	4.2880047E-4	29.795696	1
CGCAGAG	150	3.0935553E-8	28.498898	9
TTCTCCC	35	0.008324412	27.141808	68-69
CAACGCA	160	5.7889338E-8	26.717718	6
AACGCAG	160	5.7889338E-8	26.717718	7
>>END_MODULE