FastQCFastQC Report
Fri 10 Feb 2017
SRR3224489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27267
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3153995672424542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3153995672424542No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC750.2750577621300473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.16136722044962776No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA440.16136722044962776No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT430.1576997836212271No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG430.1576997836212271No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA390.1430300363076246No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT390.1430300363076246No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA390.1430300363076246No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA370.13569516265082335No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA360.1320277258224227No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG350.12836028899402208No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG320.11735797850882018No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA310.11369054168041955No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA290.10635566802361829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGATG156.549208E-494.996326
TAATGCT200.002052661771.2472464
GTGGTAT250.00489773357.2077371
CTACAGA250.00496972756.9977954
GGTATCA654.089634E-851.340281
TACATGG1651.8189894E-1234.544122
ACATGGG1651.8189894E-1234.544123
CATGGGG851.5751637E-533.5281144
ATGGGGG851.5751637E-533.5281145
GTACATG1753.6379788E-1232.6901361
AAATATG600.003884954831.6654427
GTATCAA1102.5333084E-630.3374351
CATGGGA856.248266E-427.9400964
AATCCAC350.00832441227.14180892-93
AATTCGT350.00832441227.14180812-13
CAACGCA1551.3286808E-624.5151824
ATCAACG1452.2287564E-522.930152
TGGGGGG1050.002142547922.6181746
AACGCAG1702.9673392E-622.3520775
TCAACGC1502.896387E-522.165813
Produced by FastQC (version 0.11.5)