##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27267
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07631936039902	34.0	33.0	34.0	31.0	34.0
2	33.19485091869293	34.0	33.0	34.0	31.0	34.0
3	33.313565848828254	34.0	34.0	34.0	31.0	34.0
4	36.55150915025489	37.0	37.0	37.0	35.0	37.0
5	36.594821579198296	37.0	37.0	37.0	35.0	37.0
6	36.64649576411046	37.0	37.0	37.0	35.0	37.0
7	36.62834195180988	37.0	37.0	37.0	35.0	37.0
8	36.6186599185829	37.0	37.0	37.0	35.0	37.0
9	38.43558147210914	39.0	39.0	39.0	37.0	39.0
10-11	38.4741995819122	39.0	39.0	39.0	37.0	39.0
12-13	38.46956027432428	39.0	39.0	39.0	37.0	39.0
14-15	40.04587963472329	41.0	40.0	41.0	38.0	41.0
16-17	40.04206550042176	41.0	40.0	41.0	38.0	41.0
18-19	40.06080610261488	41.0	40.0	41.0	38.0	41.0
20-21	40.05447977408589	41.0	40.0	41.0	38.0	41.0
22-23	39.94949939487292	41.0	40.0	41.0	38.0	41.0
24-25	39.91359518832289	41.0	40.0	41.0	38.0	41.0
26-27	39.821065757142335	41.0	40.0	41.0	37.5	41.0
28-29	39.7549968826787	41.0	40.0	41.0	37.0	41.0
30-31	39.637638904169876	41.0	40.0	41.0	37.0	41.0
32-33	39.50959034730627	41.0	40.0	41.0	35.5	41.0
34-35	39.363167931932374	41.0	39.5	41.0	35.0	41.0
36-37	39.196776323027834	41.0	39.0	41.0	35.0	41.0
38-39	39.04193714013276	41.0	39.0	41.0	35.0	41.0
40-41	38.83756922287014	41.0	39.0	41.0	35.0	41.0
42-43	38.680181171379324	41.0	38.0	41.0	35.0	41.0
44-45	38.30219679466021	40.0	38.0	41.0	35.0	41.0
46-47	37.920398283639564	40.0	37.0	41.0	34.0	41.0
48-49	37.686305790882756	40.0	37.0	41.0	34.0	41.0
50-51	37.45210327502109	40.0	36.5	41.0	33.0	41.0
52-53	37.1636226940991	39.5	35.5	41.0	33.0	41.0
54-55	36.91023948362489	39.0	35.0	41.0	33.0	41.0
56-57	36.67253456559211	39.0	35.0	41.0	33.0	41.0
58-59	36.37816774856053	38.0	35.0	41.0	32.0	41.0
60-61	36.084736127920195	37.5	35.0	41.0	31.0	41.0
62-63	35.87732423809	37.0	35.0	40.5	31.0	41.0
64-65	35.65183188469578	36.5	35.0	40.0	31.0	41.0
66-67	35.351102064766934	36.0	35.0	40.0	31.0	41.0
68-69	35.09669197198078	35.0	35.0	39.0	30.0	41.0
70-71	34.831609638023984	35.0	35.0	39.0	30.0	41.0
72-73	34.548208457109325	35.0	35.0	38.5	30.0	40.5
74-75	34.212509627021674	35.0	35.0	37.0	29.0	40.0
76-77	32.417574357281694	34.5	32.0	35.5	26.5	38.5
78-79	33.74219752814758	35.0	34.0	36.0	29.5	39.0
80-81	33.69138519089009	35.0	34.0	36.0	30.0	39.0
82-83	33.54131367587193	35.0	34.0	36.0	30.0	37.0
84-85	33.37549051967579	35.0	34.0	35.5	29.5	37.0
86-87	33.1724428796714	35.0	34.0	35.0	29.0	36.5
88-89	33.01534822312685	35.0	34.0	35.0	29.0	36.0
90-91	32.86564345179154	35.0	34.0	35.0	29.0	36.0
92-93	32.78052224300436	35.0	34.0	35.0	29.0	36.0
94-95	32.65757142333223	35.0	34.0	35.0	27.0	36.0
96-97	32.6131587633403	35.0	34.0	35.0	27.5	35.0
98-99	32.57523746653464	35.0	34.0	35.0	27.5	35.0
100-101	31.722228334616936	34.5	32.5	35.0	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	11.0
17	28.0
18	53.0
19	92.0
20	110.0
21	166.0
22	284.0
23	256.0
24	156.0
25	151.0
26	127.0
27	146.0
28	177.0
29	196.0
30	223.0
31	314.0
32	331.0
33	434.0
34	732.0
35	1411.0
36	4004.0
37	7686.0
38	7667.0
39	2503.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.849146984045106	18.07730572239213	12.266479973469915	23.807067320092855
2	17.658708328749036	20.52297649172993	36.53867312135549	25.279642058165546
3	20.80536912751678	25.239300253053145	28.90673708145377	25.04859353797631
4	13.206440019070673	17.03157663109253	38.049657094656546	31.71232625518026
5	13.235779513697876	37.54355081233726	34.844317306634395	14.376352367330473
6	29.81259397806873	36.21227124362783	18.55356291487879	15.421571863424651
7	25.741739098544024	31.323577951369785	22.26500898522023	20.669673964865957
8	26.42388234862654	32.724538819818825	20.52664392855833	20.324934902996297
9	26.024131734330876	14.754098360655737	21.09876407378883	38.12300583122455
10-11	26.396376572413537	24.322441045952985	27.461766971063923	21.819415410569555
12-13	26.599919316389776	23.354237723255217	27.566288920673344	22.479554039681666
14-15	23.95569736311292	24.88172516228408	25.576704441266003	25.585873033337002
16-17	22.84813144093593	27.19404408259068	26.768621410496202	23.18920306597719
18-19	22.230168335350424	25.64455202259141	29.242307551252427	22.882972090805737
20-21	23.755822055965087	24.958741335680493	29.033263652033593	22.25217295632083
22-23	23.61645945648586	25.552866101881396	28.195254336744053	22.63542010488869
24-25	23.048006748083765	24.88172516228408	28.83522206329996	23.235046026332196
26-27	22.90681043019034	25.607877654307405	28.98558697326439	22.49972494223787
28-29	22.782535345570572	25.8357324922524	28.927438431775254	22.454293730401776
30-31	22.714269996699308	25.257637437195147	29.266145890637034	22.761946675468515
32-33	22.98015916675835	25.334653610591555	29.055268273003996	22.62991894964609
34-35	23.34366346982561	25.40846826692095	28.395650340161005	22.85221792309244
36-37	22.733440915425803	25.32824763441649	28.70241326193794	23.23589818821976
38-39	23.236879744746396	25.47768364689918	27.94036747717021	23.345069131184214
40-41	23.60912458282906	25.600542780650603	28.105402134448234	22.684930502072103
42-43	23.35973887849782	25.79491693255584	28.459309788388893	22.38603440055745
44-45	23.123762013058467	25.93720196610667	27.820776171960972	23.11825984887389
46-47	23.253551631731582	25.454278477295254	27.55589001872178	23.736279872251387
48-49	23.30656104448601	25.24296768988154	27.425092602779916	24.025378662852532
50-51	23.099350863681373	26.222173323064514	26.7539516631826	23.924524150071516
52-53	23.73014999816628	26.57974841383357	26.03880148164448	23.65130010635567
54-55	23.577951369787655	25.600542780650603	26.66776689771519	24.153738951846552
56-57	23.75573079039061	26.20759215110948	26.19658903355951	23.8400880249404
58-59	23.99515694080094	26.057126084643468	26.73955715360202	23.20815982095357
60-61	23.87328723150577	26.215675844232074	26.202835812682284	23.708201111579875
62-63	23.332233102284814	26.1396559944255	27.014339677999043	23.513771225290643
64-65	24.36461656947959	25.664722925147615	26.429383503869147	23.541277001503648
66-67	23.522939817361646	26.05347122895808	25.96545274507647	24.458136208603808
68-69	23.576117651373455	26.080977005171086	26.730113323798	23.612792019657462
70-71	24.271096930355373	25.721568195987825	26.146990868082298	23.860344005574504
72-73	23.568782777716653	25.545531228224593	26.852972457549416	24.032713536509334
74-75	23.939193897385117	25.69222870136062	26.44038581435435	23.928191586899917
76-77	23.70997909561008	25.490519675798584	26.271683720247918	24.527817508343418
78-79	23.87851667094597	25.48508968198658	25.95642445805671	24.679969189010748
80-81	23.27342747111682	26.009536035209972	26.609205941683477	24.107830551989732
82-83	23.2497157736458	26.183665236366306	26.654930868815786	23.911688121172112
84-85	23.289077972566567	25.79036162253356	26.685249028093594	24.23531137680628
86-87	23.026002127113358	26.66776689771519	26.47889390105255	23.827337074118898
88-89	23.367073752154617	25.719734477573624	26.013129423845676	24.900062346426083
90-91	23.464260828107236	25.63354971210621	26.06080610261488	24.841383357171672
92-93	23.13052407672278	26.21117101257931	26.550408919206365	24.107895991491546
94-95	22.877470935563135	25.859097077052844	26.704441265999197	24.558990721384824
96-97	23.115854329409178	26.394542853999344	26.26618256500532	24.223420251586166
98-99	22.20082884072322	26.748450507940003	26.66593317930099	24.384787472035793
100-101	22.558212077285823	26.55645057707481	25.883043725572946	25.002293620066425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	2.0
5	3.0
6	2.5
7	4.0
8	7.0
9	8.0
10	7.0
11	12.0
12	17.5
13	12.0
14	14.0
15	21.5
16	19.5
17	22.0
18	24.0
19	21.5
20	35.0
21	52.0
22	59.0
23	64.0
24	86.5
25	126.0
26	159.0
27	187.5
28	234.5
29	262.0
30	262.5
31	298.5
32	358.5
33	394.0
34	429.0
35	457.0
36	487.0
37	543.0
38	572.5
39	596.5
40	633.5
41	632.5
42	651.0
43	716.5
44	766.5
45	830.0
46	879.0
47	914.5
48	972.5
49	1108.0
50	1251.0
51	1174.5
52	1061.5
53	1160.0
54	1253.5
55	1196.0
56	996.5
57	847.0
58	824.0
59	765.0
60	655.0
61	534.0
62	405.5
63	277.0
64	203.5
65	157.0
66	129.0
67	98.5
68	83.0
69	71.5
70	38.0
71	24.0
72	17.0
73	15.0
74	17.0
75	14.5
76	10.0
77	5.5
78	5.0
79	5.5
80	3.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46943191403528073
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0018337184142003153
30-31	0.0
32-33	0.0
34-35	0.0018337184142003153
36-37	0.0036674368284006307
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.018337184142003155
46-47	0.09535335753841641
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007334873656801261
58-59	0.04217552352660725
60-61	0.031173213041405363
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.014669747313602523
80-81	0.007334873656801261
82-83	0.0
84-85	0.0036674368284006307
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06418014449701104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	27267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.47093556313493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.91668244650634	69.65929511864158
2	6.234614656315092	9.660028606007263
3	1.2497633024048476	2.9046099680932995
4	0.5870100359780345	1.8190486668867127
5	0.48286309411096384	1.8703927824843216
6	0.2982389698920659	1.3862911211354385
7	0.2603673546676766	1.4119631789342428
8	0.1325506532853626	0.8215058495617412
9	0.1609543647036546	1.122235669490593
>10	0.6627532664268131	8.438772142149851
>50	0.014201855709145995	0.9058568966149557
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.3153995672424542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.3153995672424542	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	75	0.2750577621300473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.16136722044962776	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	44	0.16136722044962776	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	43	0.1576997836212271	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	43	0.1576997836212271	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	39	0.1430300363076246	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	39	0.1430300363076246	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	39	0.1430300363076246	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	37	0.13569516265082335	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	36	0.1320277258224227	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	35	0.12836028899402208	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	32	0.11735797850882018	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	31	0.11369054168041955	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	29	0.10635566802361829	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.01833718414200315	0.0
30-31	0.0	0.0	0.0	0.049510397183408515	0.0
32-33	0.0	0.0	0.0	0.07701617339641326	0.0
34-35	0.0	0.0	0.0	0.11735797850882018	0.0
36-37	0.0	0.0	0.0	0.168702094106429	0.0
38-39	0.0	0.0	0.0	0.2402171122602413	0.0
40-41	0.0	0.0	0.0	0.2878937910294495	0.0
42-43	0.0	0.0	0.0	0.33006931455605676	0.0
44-45	0.0	0.0	0.0	0.4144203616092713	0.0
46-47	0.0	0.0	0.0	0.5134411559760883	0.0
48-49	0.0	0.0	0.0	0.6197968239997066	0.0
50-51	0.0	0.0	0.0	0.7114827447097224	0.0
52-53	0.0	0.0	0.0	0.7628268603073312	0.0
54-55	0.0	0.0	0.0	0.8636813730883486	0.0
56-57	0.0	0.0	0.0	0.9700370411119669	0.0
58-59	0.0	0.0	0.0	1.0965636116917885	0.0
60-61	0.0	0.0	0.0	1.2212564638574102	0.0
62-63	0.0	0.0	0.0	1.3789562474786372	0.0
64-65	0.0	0.0	0.0	1.5128176917152603	0.0
66-67	0.0	0.0	0.0	1.698023251549492	0.0
68-69	0.0	0.0	0.0	1.866725345655921	0.0
70-71	0.0	0.0	0.0	2.0170902556203467	0.0
72-73	0.0	0.0	0.0	2.1839586313125756	0.0
74-75	0.0	0.0	0.0	2.3691641911468073	0.0
76-77	0.0	0.0	0.0	2.594711556093446	0.0
78-79	0.0	0.0	0.0	2.8129240473832837	0.0
80-81	0.0	0.0	0.0	3.111820148897935	0.0
82-83	0.0	0.0	0.0	3.4198848424835884	0.0
84-85	0.0	0.0	0.0	3.6949426046136358	0.0
86-87	0.0	0.0	0.0	4.004841016613489	0.0
88-89	0.0	0.0	0.0	4.344078923240547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGATG	15	6.549208E-4	94.99632	6
TAATGCT	20	0.0020526617	71.247246	4
GTGGTAT	25	0.004897733	57.207737	1
CTACAGA	25	0.004969727	56.997795	4
GGTATCA	65	4.089634E-8	51.34028	1
TACATGG	165	1.8189894E-12	34.54412	2
ACATGGG	165	1.8189894E-12	34.54412	3
CATGGGG	85	1.5751637E-5	33.528114	4
ATGGGGG	85	1.5751637E-5	33.528114	5
GTACATG	175	3.6379788E-12	32.690136	1
AAATATG	60	0.0038849548	31.665442	7
GTATCAA	110	2.5333084E-6	30.337435	1
CATGGGA	85	6.248266E-4	27.940096	4
AATCCAC	35	0.008324412	27.141808	92-93
AATTCGT	35	0.008324412	27.141808	12-13
CAACGCA	155	1.3286808E-6	24.515182	4
ATCAACG	145	2.2287564E-5	22.93015	2
TGGGGGG	105	0.0021425479	22.618174	6
AACGCAG	170	2.9673392E-6	22.352077	5
TCAACGC	150	2.896387E-5	22.16581	3
>>END_MODULE