FastQCFastQC Report
Fri 10 Feb 2017
SRR3224488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961889
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG29430.3059604590550469No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21820.22684530127696648No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18290.19014668012629315No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15230.15833427765573782No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14040.1459627878060774No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT13610.14149241752426736No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA12340.12828923087799113No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC12230.12714564778264437No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA12020.12496244369152783No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG11950.12423470899448896No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC11830.12298716379956524No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC11680.12142773230591057No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA11560.12018018711098682No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG10840.11269491594144437No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10210.10614530366809476No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA9670.10053135029093793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.047.0504951
GTATCAA14350.043.4292641
CCGTGCG2200.038.872989
AAACCGT2300.033.0308046
ATCAACG19350.032.145293
TCAACGC20550.030.4992474
ACCGTGC3300.030.2156778
GTCTAAT5300.029.6210981
AACCGTG2750.029.3523737
TATACGG700.00840661727.1324465
CGCAGAG23700.026.846248
TCGCACC13200.026.6698681
GTACATG38150.026.436531
ACGCAGA23900.026.4229157
TATCAAC24650.025.8115982
TACATGG38550.025.742392
CGCACCA13750.025.5538312
ACATGGG39750.024.9652613
CTGTGCG15000.024.0724539
CATGGGG28350.022.945344
Produced by FastQC (version 0.11.5)