##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224488_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961889
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18283918414703	34.0	31.0	34.0	31.0	34.0
2	32.348427937111246	34.0	31.0	34.0	31.0	34.0
3	32.49092878700141	34.0	31.0	34.0	31.0	34.0
4	35.771482988161836	37.0	37.0	37.0	35.0	37.0
5	35.77777581404923	37.0	37.0	37.0	35.0	37.0
6	35.88093948470146	37.0	37.0	37.0	35.0	37.0
7	35.84671516152071	37.0	37.0	37.0	35.0	37.0
8	35.8841009721496	37.0	37.0	37.0	35.0	37.0
9	37.60427866417019	39.0	39.0	39.0	35.0	39.0
10-11	37.681781369783835	39.0	39.0	39.0	35.0	39.0
12-13	37.67755427081504	39.0	39.0	39.0	35.0	39.0
14-15	39.171520830366084	41.0	40.0	41.0	36.0	41.0
16-17	39.00697637669211	41.0	40.0	41.0	36.0	41.0
18-19	39.083852190845306	41.0	40.0	41.0	36.0	41.0
20-21	39.13930869362265	41.0	40.0	41.0	36.0	41.0
22-23	39.09902389984707	41.0	40.0	41.0	36.0	41.0
24-25	39.040484401006765	41.0	39.5	41.0	35.5	41.0
26-27	38.86551254874523	41.0	39.0	41.0	35.0	41.0
28-29	38.7864576889849	41.0	39.0	41.0	35.0	41.0
30-31	38.71680516151032	41.0	39.0	41.0	35.0	41.0
32-33	38.52600403996719	41.0	39.0	41.0	35.0	41.0
34-35	38.51945286826235	40.0	39.0	41.0	35.0	41.0
36-37	38.51802234977217	40.0	39.0	41.0	35.0	41.0
38-39	38.402282903744606	40.0	38.0	41.0	34.5	41.0
40-41	38.299849047031415	40.0	38.0	41.0	34.0	41.0
42-43	38.14293177279291	40.0	38.0	41.0	33.5	41.0
44-45	37.95917148444363	40.0	38.0	41.0	33.0	41.0
46-47	37.779064944083984	40.0	37.0	41.0	33.0	41.0
48-49	37.55329045243266	40.0	37.0	41.0	32.5	41.0
50-51	36.264980678643795	38.5	35.0	40.0	30.5	40.5
52-53	36.59675388740281	39.0	35.5	40.0	31.0	40.5
54-55	37.03153326423319	39.0	35.0	41.0	32.0	41.0
56-57	36.868280019835964	39.0	35.0	41.0	32.0	41.0
58-59	36.70264240468495	39.0	35.0	41.0	32.0	41.0
60-61	36.44285047443104	38.5	35.0	41.0	31.0	41.0
62-63	36.139495825401895	37.5	35.0	41.0	31.0	41.0
64-65	35.88023410185583	37.0	35.0	40.0	31.0	41.0
66-67	35.630413696382846	36.5	35.0	40.0	30.0	41.0
68-69	35.314370473100325	36.0	35.0	39.5	30.0	41.0
70-71	35.0162445978694	35.5	35.0	39.0	29.5	41.0
72-73	34.69401822871454	35.0	34.5	39.0	29.0	41.0
74-75	34.41359294055759	35.0	34.0	37.5	29.0	40.0
76-77	34.08352315080015	35.0	34.0	37.0	29.0	39.0
78-79	33.85640546882229	35.0	34.0	36.5	29.0	39.0
80-81	33.567606553354906	35.0	34.0	36.0	29.0	38.5
82-83	33.32163066632428	35.0	34.0	36.0	28.0	37.0
84-85	33.10807016194176	35.0	34.0	35.0	28.0	37.0
86-87	32.905345107387646	35.0	34.0	35.0	27.0	36.5
88-89	32.77145595801595	35.0	33.5	35.0	27.0	36.0
90-91	32.61499351796309	35.0	33.0	35.0	27.0	36.0
92-93	32.484828810808736	35.0	33.0	35.0	26.5	36.0
94-95	32.36646328214586	35.0	33.0	35.0	26.0	35.0
96-97	32.244847378439715	35.0	33.0	35.0	25.0	35.0
98-99	32.18829667456433	35.0	33.0	35.0	25.0	35.0
100-101	30.98169591293798	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	211.0
8	807.0
9	538.0
10	517.0
11	1015.0
12	1731.0
13	1971.0
14	1996.0
15	2010.0
16	2119.0
17	2386.0
18	2697.0
19	2967.0
20	3446.0
21	4385.0
22	5153.0
23	5118.0
24	4906.0
25	5388.0
26	5605.0
27	5990.0
28	6671.0
29	7704.0
30	9355.0
31	11715.0
32	15640.0
33	21950.0
34	36390.0
35	65998.0
36	142391.0
37	247591.0
38	264969.0
39	70545.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07341230174597	18.558722344395576	13.159944355591097	24.207920998267358
2	16.426739467859598	19.242033124404166	38.41836220187568	25.91286520586055
3	20.37495048794713	24.954074647021947	27.937897071069678	26.733077793961247
4	13.005866581279129	16.129927673567323	37.91747280611381	32.94673293903974
5	14.104642011708213	38.40037675864887	33.7118939919263	13.783087237716618
6	31.109930563713696	36.32123872920888	18.56097741007538	14.007853297002045
7	28.07350952136889	30.685245386941734	21.550095697112663	19.691149394576712
8	26.462200940025305	34.43609397757953	20.014783410559847	19.086921671835317
9	26.576143401161673	15.59202777035604	19.664950945483316	38.166877882998975
10-11	26.475819975069886	24.79267358291861	27.13239261494829	21.599113827063206
12-13	26.814684438641052	22.29420442483488	27.293637831392186	23.59747330513188
14-15	23.89861558965751	24.277543515350818	24.599426850062166	27.224414044929507
16-17	23.869115122509495	27.059147895749884	25.707121677157573	23.364615304583054
18-19	23.796606820675205	26.50095930368591	26.333224144295915	23.369209731342973
20-21	24.895748342035922	24.8706312198029	26.251344910090584	23.98227552807059
22-23	24.815562120051204	25.73911695569694	25.362256646811925	24.08306427743993
24-25	24.132009522537	25.505904274483647	26.074417826006258	24.287668376973095
26-27	24.11660804936952	26.398576135084195	26.06969203307242	23.415123782473863
28-29	23.81012777981659	26.54116015465402	26.14693587305812	23.501776192471272
30-31	23.912426485800335	25.608672102498314	26.375080700579794	24.103820711121553
32-33	23.748738160016384	25.86447084850747	26.42732165561723	23.95946933585892
34-35	24.409989094375753	25.403814785281874	26.051186779347724	24.13500934099465
36-37	23.71408026928002	25.53402889020257	26.70588987657479	24.046000963942618
38-39	23.818536820687914	26.006225587079722	25.8465831528627	24.328654439369664
40-41	24.593041812958823	25.47474556340979	26.28273574047832	23.64947688315307
42-43	24.347771391975513	25.672241998802313	26.257516717679152	23.722469891543017
44-45	24.36002543210403	25.36893966529646	25.97947652079251	24.291558381807
46-47	24.159908263843334	25.356512029974354	25.915412277300188	24.568167428882127
48-49	23.723267445620024	25.7347261482354	26.27247010829732	24.269536297847257
50-51	23.891530103785364	25.94701675557159	25.86504264005514	24.296410500587907
52-53	24.22748362856837	25.977685574946797	25.265857079143228	24.528973717341607
54-55	24.25524150915542	25.218606304885494	25.80484858440007	24.721303601559015
56-57	24.146405742453517	25.736402919881048	25.714830773005392	24.402360564660043
58-59	24.45951678846082	25.77994701226537	26.029444793351995	23.731091405921816
60-61	24.305017598768867	26.014879668092938	25.58559448485258	24.094508248285614
62-63	23.908248465833402	25.62071221104059	26.13579285293111	24.335246470194893
64-65	24.774020216876107	25.04008375072614	25.60050217151737	24.58539386088039
66-67	23.76984533415963	25.708874270381614	25.36631041203845	25.154969983420305
68-69	24.05199560448243	25.76575883495913	26.275329066035685	23.906916494522758
70-71	24.438162823361115	25.32256840446247	25.99899780536008	24.24027096681634
72-73	24.14010348387392	25.48729635124219	26.269091340061067	24.10350882482282
74-75	24.24027096681634	25.29569420172182	26.23946214168163	24.224572689780214
76-77	24.085991211044103	24.95131974687308	25.773192125078882	25.18949691700394
78-79	24.163139128997138	24.70227790030144	26.169910337635006	24.964672633066414
80-81	24.00941833412825	25.671045904236532	25.65960326281719	24.659932498818026
82-83	23.94417399987518	25.90671742703197	25.882065364372046	24.2670432087208
84-85	24.064416093311923	25.12692493281974	26.450355244220003	24.358303729648334
86-87	23.884462286089672	26.087684704979157	25.7628109537484	24.265042055182768
88-89	24.18881479601812	25.264675172324857	25.543806080977085	25.002703950679937
90-91	23.74889410316575	25.28867676000037	25.839000134111107	25.12342900272277
92-93	23.668291475911428	25.456032162801833	26.40295095994049	24.472725401346253
94-95	24.055894183216566	25.199685202762478	26.234264036702783	24.510156577318174
96-97	24.197958392288506	25.761756294125416	26.070107881470733	23.970177432115346
98-99	23.278363001717985	25.874002281983504	26.227891660363923	24.61974305593459
100-101	24.003118253902628	25.3202632937935	25.71586965532269	24.96074879698118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.5
4	2.5
5	4.0
6	4.0
7	7.5
8	15.0
9	21.0
10	24.0
11	23.5
12	37.5
13	39.5
14	35.0
15	45.5
16	58.5
17	70.0
18	81.0
19	91.5
20	119.5
21	149.0
22	169.5
23	236.5
24	315.5
25	425.0
26	615.5
27	878.5
28	1205.0
29	1790.0
30	3028.0
31	4950.0
32	7461.5
33	10514.0
34	13870.5
35	17062.5
36	19966.0
37	22579.5
38	23866.0
39	24714.5
40	25352.0
41	25585.0
42	26120.0
43	27189.0
44	28893.5
45	31786.0
46	35440.0
47	36603.5
48	38079.5
49	41882.0
50	45329.0
51	45194.0
52	43902.5
53	44239.0
54	45296.0
55	45126.0
56	38450.5
57	32483.5
58	30349.0
59	28013.5
60	23596.0
61	18029.5
62	14764.5
63	10752.0
64	6630.0
65	4423.0
66	3442.0
67	2898.0
68	2190.5
69	1464.0
70	928.5
71	647.5
72	517.5
73	435.0
74	333.0
75	263.0
76	218.0
77	147.5
78	101.5
79	77.0
80	53.0
81	31.0
82	21.0
83	21.0
84	14.5
85	8.5
86	6.5
87	8.0
88	10.0
89	8.0
90	7.5
91	7.0
92	5.0
93	7.0
94	7.0
95	4.5
96	6.5
97	5.5
98	3.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1564629598633522
2	0.0
3	2.0792419915395647E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03747833689750065
16-17	0.0768279915873869
18-19	0.026406373292552465
20-21	0.04090908618354093
22-23	0.06835508047186317
24-25	0.017725537977874788
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02219590825968485
38-39	0.005406029178002867
40-41	0.006029801775464736
42-43	0.0034307492860402816
44-45	0.011695736202410049
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.1981049788489116E-5
58-59	0.016322049633585578
60-61	0.01808940532639421
62-63	0.030356933076477643
64-65	0.04829039525350638
66-67	0.017725537977874788
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0176735569280863
80-81	0.059518302007820025
82-83	0.05291670868468191
84-85	0.03087674357436253
86-87	0.0615975439993596
88-89	0.03461937915913375
90-91	0.0
92-93	0.0033787682362517923
94-95	0.0
96-97	0.0
98-99	6.757536472503584E-4
100-101	0.04714681215815962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	961889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.20633112380935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35960854087489	41.39174225051125
2	14.154349056143408	15.34510663558576
3	4.474501809610188	7.276389801174419
4	1.931304726577214	4.187557740392902
5	0.9593292293842166	2.6000858932374844
6	0.5423023888333246	1.7637733714999198
7	0.3248697741581758	1.2326998985094668
8	0.21523223667923322	0.9333559911962096
9	0.14422576607025414	0.7036154668970337
>10	0.7206748001947076	7.092183106282396
>50	0.08099198326347894	3.068730296427389
>100	0.08117878537201094	9.175525186033614
>500	0.008524741100227946	2.949425968619289
>1k	0.0029061617387140725	2.2798083936329414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2943	0.3059604590550469	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2182	0.22684530127696648	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1829	0.19014668012629315	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1523	0.15833427765573782	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1404	0.1459627878060774	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1361	0.14149241752426736	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1234	0.12828923087799113	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1223	0.12714564778264437	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1202	0.12496244369152783	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1195	0.12423470899448896	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1183	0.12298716379956524	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1168	0.12142773230591057	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1156	0.12018018711098682	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1084	0.11269491594144437	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1021	0.10614530366809476	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	967	0.10053135029093793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.0792419915395644E-4
7	0.0	0.0	0.0	0.0	2.0792419915395644E-4
8	0.0	0.0	0.0	0.0	2.0792419915395644E-4
9	0.0	0.0	0.0	0.0	2.0792419915395644E-4
10-11	0.0	0.0	0.0	1.0396209957697822E-4	2.0792419915395644E-4
12-13	0.0	0.0	0.0	1.0396209957697822E-4	2.0792419915395644E-4
14-15	0.0	0.0	0.0	2.0792419915395644E-4	2.5990524894244554E-4
16-17	0.0	0.0	0.0	3.6386734851942375E-4	3.1188629873093467E-4
18-19	0.0	0.0	0.0	4.158483983079129E-4	3.1188629873093467E-4
20-21	0.0	0.0	0.0	6.237725974618693E-4	3.1188629873093467E-4
22-23	0.0	0.0	0.0	7.277346970388475E-4	3.1188629873093467E-4
24-25	0.0	0.0	0.0	9.87639945981293E-4	3.1188629873093467E-4
26-27	0.0	0.0	0.0	0.001871317792385608	3.1188629873093467E-4
28-29	0.0	0.0	0.0	0.0031708440370978356	3.1188629873093467E-4
30-31	0.0	0.0	0.0	0.0062377259746186926	3.1188629873093467E-4
32-33	0.0	0.0	0.0	0.011331868853890625	4.158483983079129E-4
34-35	0.0	0.0	0.0	0.01933695052131795	5.198104978848911E-4
36-37	0.0	0.0	0.0	0.03430749286040281	5.198104978848911E-4
38-39	0.0	0.0	0.0	0.055827647472837305	5.198104978848911E-4
40-41	0.0	0.0	0.0	0.08270185021348617	5.198104978848911E-4
42-43	0.0	0.0	0.0	0.11004388240223145	5.198104978848911E-4
44-45	0.0	0.0	0.0	0.1370220472424573	5.198104978848911E-4
46-47	0.0	0.0	0.0	0.1746563272893234	5.198104978848911E-4
48-49	0.0	0.0	0.0	0.21213466418682406	6.237725974618693E-4
50-51	0.0	0.0	0.0	0.2539274282167693	6.237725974618693E-4
52-53	0.0	0.0	0.0	0.29712368059100375	6.757536472503584E-4
54-55	0.0	0.0	0.0	0.3418793644588929	7.277346970388475E-4
56-57	0.0	0.0	0.0	0.3967193719857489	7.277346970388475E-4
58-59	0.0	0.0	0.0	0.45899266963235885	7.277346970388475E-4
60-61	0.0	0.0	0.0	0.5273477501042221	7.277346970388475E-4
62-63	0.0	0.0	0.0	0.5963785842233356	7.277346970388475E-4
64-65	0.0	0.0	0.0	0.6693599781263742	7.277346970388475E-4
66-67	0.0	0.0	0.0	0.7485271169542431	7.277346970388475E-4
68-69	0.0	0.0	0.0	0.8432365896688703	7.277346970388475E-4
70-71	0.0	0.0	0.0	0.9473026513454255	7.277346970388475E-4
72-73	0.0	0.0	0.0	1.0594257757391965	0.0010396209957697822
74-75	0.0	0.0	0.0	1.1761232325143545	0.0010396209957697822
76-77	0.0	5.198104978848911E-5	0.0	1.2958355901772451	0.0010396209957697822
78-79	0.0	1.0396209957697822E-4	0.0	1.4377958371496087	0.0010396209957697822
80-81	0.0	1.0396209957697822E-4	0.0	1.5969098305521738	0.0011435830953467605
82-83	0.0	1.0396209957697822E-4	0.0	1.7814425573013102	0.0012475451949237387
84-85	0.0	1.0396209957697822E-4	0.0	1.9634282126108107	0.0012475451949237387
86-87	0.0	1.0396209957697822E-4	0.0	2.162723557499878	0.0012475451949237387
88-89	0.0	1.0396209957697822E-4	0.0	2.3831232086030716	0.0012475451949237387
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	910	0.0	47.050495	1
GTATCAA	1435	0.0	43.429264	1
CCGTGCG	220	0.0	38.87298	9
AAACCGT	230	0.0	33.030804	6
ATCAACG	1935	0.0	32.14529	3
TCAACGC	2055	0.0	30.499247	4
ACCGTGC	330	0.0	30.215677	8
GTCTAAT	530	0.0	29.621098	1
AACCGTG	275	0.0	29.352373	7
TATACGG	70	0.008406617	27.132446	5
CGCAGAG	2370	0.0	26.84624	8
TCGCACC	1320	0.0	26.669868	1
GTACATG	3815	0.0	26.43653	1
ACGCAGA	2390	0.0	26.422915	7
TATCAAC	2465	0.0	25.811598	2
TACATGG	3855	0.0	25.74239	2
CGCACCA	1375	0.0	25.553831	2
ACATGGG	3975	0.0	24.965261	3
CTGTGCG	1500	0.0	24.072453	9
CATGGGG	2835	0.0	22.94534	4
>>END_MODULE