FastQCFastQC Report
Fri 10 Feb 2017
SRR3224488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961889
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG18430.19160214952037086No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA16410.17060180540582123No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16090.16727501821935795No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT15890.1651957762278184No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15210.15812635345658388No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG13690.14232411432088318No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT12770.13275960115980118No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12540.13036847286953068No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG12220.12704168568306737No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG11470.119244528214794No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11100.11539793053044582No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC11010.11446227163425302No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG10740.11165529494567461No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG10520.10936812875498107No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC10450.10864039405794224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.045.9371221
CCGTGCG2600.042.0095069
ACCGTGC2750.041.444958
GTATCAA15750.040.039671
AAACCGT2500.039.8907666
ATCAACG21650.029.3927333
AACCGTG3400.029.3314447
CGATTAT2500.028.4934022
TCAACGC22700.028.033164
GTACATG43550.027.4251461
TACATGG44800.026.3945582
ACATGGG44100.026.1674123
GTGCGCG2200.025.90309310-11
TGGTATC5800.025.3820552
GGACCGT1351.344842E-524.6239286
CAACGCA26400.024.284155
ACGCAGA26600.024.1015627
GTATTAT2602.1827873E-1123.8873021
CGCAGAG27150.023.613328
TCCGGGC8600.023.1923078
Produced by FastQC (version 0.11.5)