##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224488_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 961889 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.14876144752669 34.0 33.0 34.0 31.0 34.0 2 33.277801284763626 34.0 33.0 34.0 31.0 34.0 3 33.39245276741911 34.0 34.0 34.0 31.0 34.0 4 36.610205543467075 37.0 37.0 37.0 35.0 37.0 5 36.63813600113942 37.0 37.0 37.0 35.0 37.0 6 36.68036852485058 37.0 37.0 37.0 36.0 37.0 7 36.657541566646465 37.0 37.0 37.0 35.0 37.0 8 36.644055603089335 37.0 37.0 37.0 35.0 37.0 9 38.481249915530796 39.0 39.0 39.0 37.0 39.0 10-11 38.50475002832967 39.0 39.0 39.0 37.0 39.0 12-13 38.496533383789604 39.0 39.0 39.0 37.0 39.0 14-15 40.09510037020904 41.0 40.0 41.0 38.0 41.0 16-17 40.10220046179965 41.0 40.0 41.0 38.0 41.0 18-19 40.08742900688125 41.0 40.0 41.0 38.0 41.0 20-21 40.087064099911736 41.0 40.0 41.0 38.0 41.0 22-23 40.01150964404417 41.0 40.0 41.0 38.0 41.0 24-25 39.974185171054046 41.0 40.0 41.0 38.0 41.0 26-27 39.90588363106346 41.0 40.0 41.0 38.0 41.0 28-29 39.87201849693676 41.0 40.0 41.0 38.0 41.0 30-31 39.818367296018565 41.0 40.0 41.0 38.0 41.0 32-33 39.774709971732705 41.0 40.0 41.0 37.0 41.0 34-35 39.69326814216609 41.0 40.0 41.0 37.0 41.0 36-37 39.582734598274854 41.0 40.0 41.0 37.0 41.0 38-39 39.46649301530634 41.0 39.5 41.0 36.0 41.0 40-41 39.32905252061309 41.0 39.0 41.0 36.0 41.0 42-43 39.230177286568406 41.0 39.0 41.0 35.0 41.0 44-45 38.978735592152525 41.0 38.5 41.0 35.0 41.0 46-47 38.7496431500932 40.0 38.0 41.0 35.0 41.0 48-49 38.638343925338575 40.0 38.0 41.0 35.0 41.0 50-51 38.46676747524923 40.0 37.5 41.0 35.0 41.0 52-53 38.21712952326099 40.0 37.0 41.0 35.0 41.0 54-55 38.01208923274931 40.0 36.5 41.0 34.5 41.0 56-57 37.77457845967674 39.5 35.5 41.0 34.0 41.0 58-59 37.49385999839898 39.0 35.0 41.0 34.0 41.0 60-61 37.232458734843625 39.0 35.0 41.0 34.0 41.0 62-63 37.004531188110064 38.0 35.0 41.0 33.0 41.0 64-65 36.724012334063495 37.0 35.0 40.5 33.0 41.0 66-67 36.43346997418621 37.0 35.0 40.0 33.0 41.0 68-69 36.1565508078375 36.0 35.0 39.5 33.0 41.0 70-71 35.850731217427374 36.0 35.0 39.0 32.5 41.0 72-73 35.53923893505384 35.0 35.0 39.0 32.0 41.0 74-75 35.22592471688521 35.0 35.0 37.5 32.0 40.0 76-77 33.41042729462547 34.5 32.0 35.5 28.5 38.5 78-79 34.70913015950905 35.0 34.5 37.0 32.0 39.0 80-81 34.63481129319496 35.0 35.0 36.0 32.0 39.0 82-83 34.444089182847506 35.0 35.0 36.0 32.0 37.0 84-85 34.25187885504461 35.0 35.0 36.0 32.0 37.0 86-87 34.06424857753858 35.0 35.0 35.0 32.0 36.5 88-89 33.926516469156006 35.0 34.5 35.0 32.0 36.0 90-91 33.788544728133914 35.0 34.0 35.0 31.0 36.0 92-93 33.69959631516734 35.0 34.0 35.0 31.0 36.0 94-95 33.608176203283335 35.0 34.0 35.0 31.0 36.0 96-97 33.564584894930704 35.0 34.0 35.0 31.0 35.0 98-99 33.53143450023859 35.0 34.0 35.0 31.0 35.0 100-101 32.67453261239083 34.5 33.0 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 22.0 15 53.0 16 109.0 17 236.0 18 431.0 19 701.0 20 1048.0 21 1616.0 22 2310.0 23 2394.0 24 2213.0 25 2693.0 26 3354.0 27 4643.0 28 5954.0 29 6868.0 30 7641.0 31 8702.0 32 10360.0 33 13927.0 34 23841.0 35 48519.0 36 133595.0 37 275878.0 38 315245.0 39 89506.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58860395884473 18.465660416775474 13.101373583328982 23.844362041050818 2 16.347312423782785 19.366579719697388 38.784516716585806 25.50159113993403 3 20.18777634425594 25.330781410329053 28.01300357941509 26.468438665999923 4 13.11596244473115 16.28545497453448 38.220938174779 32.377644405955365 5 14.142484215954232 37.63365627426865 34.353132222117104 13.870727287660012 6 31.22491264584583 35.91277163997093 18.75549049838391 14.10682521579933 7 28.15979806401778 30.49489078261629 21.660399484763833 19.684911668602094 8 26.611802401316574 34.03126556182678 19.818087118160204 19.53884491869644 9 26.64361480378713 15.627686770510943 19.7783735961218 37.950324829580126 10-11 26.656246198885736 24.64863409395471 27.23157245794473 21.463547249214827 12-13 26.98804123968566 22.569807950813452 27.22580256141821 23.21634824808268 14-15 23.923134582056765 24.608192837219264 24.79402509021311 26.67464749051086 16-17 23.73064875468999 27.15422465585946 25.70722817289729 23.40789841655326 18-19 23.666504139250993 26.521303393634817 26.81125368935501 23.00093877775918 20-21 24.743679578160314 25.266040948889778 26.566408353623416 23.423871119326485 22-23 24.788694176796177 25.700817984854595 25.790538489368693 23.719949348980535 24-25 24.518823667008878 25.350313836077504 26.080908630167247 24.04995386674637 26-27 24.164321838172263 26.52296025912066 26.080228222464918 23.232489680242153 28-29 23.873066502539874 26.40862788478133 26.565459766410427 23.15284584626837 30-31 23.927402775287003 25.53601921232583 26.65840162389897 23.8781763884882 32-33 23.914399508466172 25.815610357557595 26.83132218233535 23.43866795164089 34-35 24.60425386805066 25.533189028688348 26.04594876145444 23.816608341806553 36-37 23.72033788174139 25.50040285899935 26.71740090968161 24.061858349577648 38-39 23.898079716058714 25.8923326911941 25.97550237085568 24.234085221891508 40-41 24.130590951762628 25.79518010913941 26.430856367002846 23.643372572095117 42-43 24.14909620548733 25.800378214118265 26.561380783021743 23.489144797372667 44-45 24.319558495407577 25.375389113351815 26.280253108228095 24.024799283012513 46-47 24.046790537640675 25.38515681207989 26.15965819560114 24.4083944546783 48-49 23.692679399697365 25.381660838679192 26.67495944146966 24.250700320153783 50-51 24.06436709433209 25.56064161249375 26.291443191470115 24.083548101704043 52-53 24.281595901398187 25.904132389496088 25.755206681852066 24.059065027253666 54-55 24.36154275597288 25.102220734409066 26.23935817958205 24.296878330036 56-57 24.41066781450371 25.567394357923455 25.841201319596717 24.180736507976118 58-59 24.679314671779533 25.62014070788683 26.030075023752065 23.670469596581576 60-61 24.17425170094556 26.121584134734128 25.735507839818634 23.968656324501676 62-63 24.007083977465175 25.539589287329413 26.271482468351337 24.181844266854075 64-65 24.397929490824826 25.23487637346929 25.930746686987792 24.436447448718095 66-67 23.87988634863274 25.543279941864395 25.877310167805224 24.699523541697637 68-69 24.33342100803731 25.515470080227555 26.19917682809555 23.951932083639587 70-71 24.61786131247992 25.372990022757303 26.111536778152157 23.89761188661062 72-73 24.1731634315394 25.385205569457597 26.319980787804 24.121650211199004 74-75 24.29142031980821 25.336447344755996 26.216226612426176 24.155905723009617 76-77 24.215135681359328 24.760314631879453 26.034582060659993 24.989967626101222 78-79 24.211479008729835 24.813724863318523 26.019048331984322 24.955747795967316 80-81 23.7270126175881 25.687490123015245 25.987230618236868 24.598266641159785 82-83 23.81412790519365 25.682091204016317 26.35738579744905 24.146395093340985 84-85 24.15768067967746 25.059259690518864 26.50711948001181 24.275940149791865 86-87 23.720096601582927 25.945145437779203 26.083622954415738 24.251135006222132 88-89 24.301400681367603 25.11916655664011 25.47107826370818 25.108354498284108 90-91 24.163598918378316 25.36581663788649 25.66928200655169 24.8013024371835 92-93 24.11246209929737 25.495470628872262 26.20651977853982 24.185547493290542 94-95 24.036661194794824 25.271419051470595 26.02322097456151 24.668698779173067 96-97 24.247548313786726 25.703225632063575 26.042298019833886 24.006928034315813 98-99 22.93045247424599 26.04338962187945 26.391350769163598 24.63480713471097 100-101 23.700631270572472 25.588044975427472 25.880845078627935 24.830478675372124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.5 2 0.5 3 1.0 4 2.0 5 4.5 6 6.0 7 9.5 8 12.5 9 16.0 10 26.5 11 25.5 12 31.5 13 44.5 14 49.5 15 55.0 16 65.5 17 77.5 18 89.5 19 102.0 20 119.5 21 155.5 22 189.0 23 243.5 24 334.0 25 476.5 26 669.5 27 928.5 28 1307.5 29 1974.0 30 3162.0 31 5038.5 32 7531.5 33 10639.5 34 13879.5 35 16951.0 36 20159.5 37 22604.0 38 23686.5 39 24536.0 40 25359.5 41 25851.0 42 26470.5 43 27798.5 44 29685.0 45 32426.5 46 35204.5 47 37184.0 48 38834.0 49 42274.5 50 46859.0 51 46509.5 52 43152.5 53 43219.5 54 45476.5 55 44856.0 56 38074.0 57 32050.5 58 30177.5 59 28557.0 60 23916.0 61 17781.0 62 14128.5 63 10055.0 64 6093.0 65 3786.5 66 2751.5 67 2331.5 68 1800.5 69 1179.5 70 725.0 71 479.5 72 385.5 73 351.0 74 284.5 75 226.0 76 153.0 77 86.5 78 52.0 79 30.0 80 22.5 81 17.5 82 9.5 83 6.0 84 5.0 85 2.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47188396997990417 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.277346970388475E-4 22-23 0.0013515072945007168 24-25 1.5594314936546734E-4 26-27 0.0021312230413280536 28-29 0.003222825086886325 30-31 6.757536472503584E-4 32-33 2.0792419915395647E-4 34-35 0.003638673485194238 36-37 0.001455469394077695 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.009044702663197105 46-47 0.07843940413083006 48-49 7.797157468273366E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008316967966158257 58-59 0.04090908618354093 60-61 0.030304952026689147 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.1188629873093467E-4 78-79 0.007537252219330921 80-81 0.0059258396758877585 82-83 0.0012995262447122278 84-85 0.0021832040911165425 86-87 0.0 88-89 0.0 90-91 0.0 92-93 5.1981049788489116E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.06788725102376678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 961889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.51316211966074 #Duplication Level Percentage of deduplicated Percentage of total 1 75.34748215978756 39.56734545965169 2 14.404185702457545 15.128186779897046 3 4.838556779540674 7.62263749767609 4 2.0356329726672855 4.275900972392163 5 1.0413011534225967 2.734100814253527 6 0.5968587121138292 1.8805762987059254 7 0.3769762431082642 1.3857350198723442 8 0.2342087411011718 0.9839233273029987 9 0.17689392706255355 0.8360333522837384 >10 0.7622768996973945 7.363672866868412 >50 0.08657641322804395 3.2597252594477073 >100 0.08795666276258468 9.987293056901324 >500 0.008122124197730422 2.9094798301093143 >1k 0.002971508852828203 2.0653894646378124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 1843 0.19160214952037086 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 1641 0.17060180540582123 No Hit GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA 1609 0.16727501821935795 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 1589 0.1651957762278184 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 1521 0.15812635345658388 No Hit CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG 1369 0.14232411432088318 No Hit GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT 1277 0.13275960115980118 No Hit ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG 1254 0.13036847286953068 No Hit TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG 1222 0.12704168568306737 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG 1147 0.119244528214794 No Hit ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT 1110 0.11539793053044582 No Hit TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC 1101 0.11446227163425302 No Hit GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG 1074 0.11165529494567461 No Hit GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG 1052 0.10936812875498107 No Hit GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1045 0.10864039405794224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.0396209957697822E-4 3 0.0 0.0 0.0 0.0 1.0396209957697822E-4 4 0.0 0.0 0.0 0.0 1.0396209957697822E-4 5 0.0 0.0 0.0 0.0 1.0396209957697822E-4 6 0.0 0.0 0.0 0.0 2.0792419915395644E-4 7 0.0 0.0 0.0 0.0 2.0792419915395644E-4 8 0.0 0.0 0.0 0.0 4.158483983079129E-4 9 0.0 0.0 0.0 1.0396209957697822E-4 4.158483983079129E-4 10-11 0.0 0.0 0.0 3.1188629873093467E-4 4.158483983079129E-4 12-13 0.0 0.0 0.0 3.1188629873093467E-4 4.158483983079129E-4 14-15 0.0 0.0 0.0 3.6386734851942375E-4 4.67829448096402E-4 16-17 0.0 0.0 0.0 5.198104978848911E-4 5.198104978848911E-4 18-19 0.0 0.0 0.0 6.237725974618693E-4 5.198104978848911E-4 20-21 0.0 0.0 0.0 7.277346970388475E-4 5.198104978848911E-4 22-23 0.0 0.0 0.0 7.797157468273366E-4 5.717915476733803E-4 24-25 0.0 0.0 0.0 9.87639945981293E-4 6.237725974618693E-4 26-27 0.0 0.0 0.0 0.001871317792385608 6.237725974618693E-4 28-29 0.0 0.0 0.0 0.0030668819375208574 6.237725974618693E-4 30-31 0.0 0.0 0.0 0.006081782825253226 6.237725974618693E-4 32-33 0.0 0.0 0.0 0.011331868853890627 6.237725974618693E-4 34-35 0.0 0.0 0.0 0.019492893670683416 6.237725974618693E-4 36-37 0.0 0.0 0.0 0.03446343600976828 7.277346970388475E-4 38-39 0.0 0.0 0.0 0.056347457970722194 8.316967966158258E-4 40-41 0.0 0.0 0.0 0.08389741435862141 8.316967966158258E-4 42-43 0.0 0.0 0.0 0.11243501069250195 8.316967966158258E-4 44-45 0.0 0.0 0.0 0.13962109973188175 8.836778464043148E-4 46-47 0.0 0.0 0.0 0.17741132292811335 9.356588961928039E-4 48-49 0.0 0.0 0.0 0.2152535271741334 9.356588961928039E-4 50-51 0.0 0.0 0.0 0.2577740259011175 9.356588961928039E-4 52-53 0.0 0.0 0.0 0.30180197507196777 0.0010916020455582712 54-55 0.0 0.0 0.0 0.34671360208922236 0.0012475451949237387 56-57 0.0 0.0 0.0 0.4023853064126942 0.0012475451949237387 58-59 0.0 0.0 0.0 0.46549030085591997 0.0012475451949237387 60-61 0.0 0.0 0.0 0.534781040223976 0.0012475451949237387 62-63 0.0 0.0 0.0 0.6047995142890707 0.0012475451949237387 64-65 0.0 0.0 0.0 0.6785606239389368 0.0012475451949237387 66-67 0.0 0.0 0.0 0.7586634216629985 0.0012475451949237387 68-69 0.0 0.0 0.0 0.8543605343236069 0.0012475451949237387 70-71 0.0 0.0 0.0 0.959466216995932 0.0012475451949237387 72-73 0.0 0.0 0.0 1.0723690571365303 0.0013515072945007168 74-75 0.0 0.0 0.0 1.1906259454053432 0.0013515072945007168 76-77 1.0396209957697822E-4 0.0 0.0 1.3120536777112535 0.0013515072945007168 78-79 1.0396209957697822E-4 0.0 0.0 1.455885242476003 0.0013515072945007168 80-81 1.0396209957697822E-4 0.0 0.0 1.6172344210194733 0.0015074504438661843 82-83 1.0396209957697822E-4 0.0 0.0 1.803846389760149 0.0020792419915395643 84-85 1.0396209957697822E-4 0.0 0.0 1.9882751544097084 0.0020792419915395643 86-87 1.0396209957697822E-4 0.0 0.0 2.1909492675350277 0.0020792419915395643 88-89 1.0396209957697822E-4 0.0 0.0 2.4141558953267994 0.0020792419915395643 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1040 0.0 45.937122 1 CCGTGCG 260 0.0 42.009506 9 ACCGTGC 275 0.0 41.44495 8 GTATCAA 1575 0.0 40.03967 1 AAACCGT 250 0.0 39.890766 6 ATCAACG 2165 0.0 29.392733 3 AACCGTG 340 0.0 29.331444 7 CGATTAT 250 0.0 28.493402 2 TCAACGC 2270 0.0 28.03316 4 GTACATG 4355 0.0 27.425146 1 TACATGG 4480 0.0 26.394558 2 ACATGGG 4410 0.0 26.167412 3 GTGCGCG 220 0.0 25.903093 10-11 TGGTATC 580 0.0 25.382055 2 GGACCGT 135 1.344842E-5 24.623928 6 CAACGCA 2640 0.0 24.28415 5 ACGCAGA 2660 0.0 24.101562 7 GTATTAT 260 2.1827873E-11 23.887302 1 CGCAGAG 2715 0.0 23.61332 8 TCCGGGC 860 0.0 23.192307 8 >>END_MODULE