FastQCFastQC Report
Fri 10 Feb 2017
SRR3224486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705362
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA10090.14304711623251606No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG9410.13340667628820377No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC9390.13312313393690048No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9090.12886999866735097No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA8270.11724476226391556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1700.053.10529
ACCGTGC2000.047.4848638
AAACCGT2250.046.4263576
GTATCAA9700.043.6335451
GGTATCA5250.043.4794651
AACCGTG2200.043.1649977
ATCAACG11950.034.1707883
TCAACGC12250.033.333954
TATCAAC14950.027.9489752
GTAATCG350.00837333627.15538414-15
ATGCGGG2155.820766E-1126.503188
ACGCAGA15300.026.3786097
CGCAGAG15600.025.8731638
TACATGG21900.025.583642
CAACGCA16300.025.0515885
AATGCGG1901.1943484E-824.9902617
CGGCTTA2000.024.94900916-17
GTACATG22350.024.682131
TGCGCGG1850.024.40314912-13
AACGCAG17000.023.740756
Produced by FastQC (version 0.11.5)