##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224486_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705362
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.120725244626165	34.0	31.0	34.0	30.0	34.0
2	32.289374817469614	34.0	31.0	34.0	31.0	34.0
3	32.43558484863092	34.0	31.0	34.0	31.0	34.0
4	35.72689767807169	37.0	35.0	37.0	35.0	37.0
5	35.73772899589147	37.0	37.0	37.0	35.0	37.0
6	35.8430990044828	37.0	37.0	37.0	35.0	37.0
7	35.79984320107973	37.0	37.0	37.0	35.0	37.0
8	35.83746501796241	37.0	37.0	37.0	35.0	37.0
9	37.54289995775219	39.0	38.0	39.0	35.0	39.0
10-11	37.62113921645907	39.0	38.5	39.0	35.0	39.0
12-13	37.62743456551388	39.0	38.5	39.0	35.0	39.0
14-15	39.11098556485889	41.0	40.0	41.0	36.0	41.0
16-17	38.94163847783125	41.0	39.5	41.0	35.5	41.0
18-19	39.02941326581245	41.0	39.5	41.0	36.0	41.0
20-21	39.10225246043875	41.0	40.0	41.0	36.0	41.0
22-23	39.05332722772137	41.0	40.0	41.0	36.0	41.0
24-25	38.9942717356478	41.0	39.0	41.0	35.5	41.0
26-27	38.82224233797681	41.0	39.0	41.0	35.0	41.0
28-29	38.73548617589266	41.0	39.0	41.0	35.0	41.0
30-31	38.65209920579787	41.0	39.0	41.0	35.0	41.0
32-33	38.45427666928471	40.0	39.0	41.0	34.0	41.0
34-35	38.45055588477973	40.0	38.0	41.0	34.0	41.0
36-37	38.44910840107633	40.0	38.0	41.0	34.0	41.0
38-39	38.352881215602764	40.0	38.0	41.0	34.0	41.0
40-41	38.2667254544475	40.0	38.0	41.0	34.0	41.0
42-43	38.128831011594045	40.0	38.0	41.0	33.0	41.0
44-45	37.96087540865541	40.0	38.0	41.0	33.0	41.0
46-47	37.799741125833265	40.0	38.0	41.0	33.0	41.0
48-49	37.60962456157264	40.0	37.0	41.0	32.5	41.0
50-51	36.36010445700222	38.5	35.5	40.0	30.5	40.5
52-53	36.7221632296608	39.0	36.0	40.0	31.0	40.5
54-55	37.21960638650792	40.0	36.0	41.0	32.0	41.0
56-57	37.09877694006765	40.0	35.5	41.0	32.0	41.0
58-59	36.967403262438296	39.5	35.0	41.0	31.5	41.0
60-61	36.72709899314111	39.0	35.0	41.0	31.0	41.0
62-63	36.44015413362217	39.0	35.0	41.0	31.0	41.0
64-65	36.18487670160854	38.0	35.0	41.0	30.0	41.0
66-67	35.92124540306963	37.5	35.0	40.0	30.0	41.0
68-69	35.58942642217755	37.0	35.0	40.0	29.5	41.0
70-71	35.2663930010406	36.0	35.0	39.5	29.0	41.0
72-73	34.92008855027631	36.0	34.5	39.0	29.0	41.0
74-75	34.609716712836814	35.5	34.0	39.0	29.0	40.5
76-77	34.22969340565554	35.0	34.0	37.0	29.0	39.5
78-79	33.96358961781327	35.0	34.0	37.0	29.0	39.0
80-81	33.62731476887045	35.0	34.0	36.5	28.0	39.0
82-83	33.351537224857594	35.0	34.0	36.0	27.0	37.5
84-85	33.11928130520215	35.0	34.0	36.0	27.0	37.0
86-87	32.87591052537562	35.0	34.0	35.0	27.0	37.0
88-89	32.70432628919619	35.0	33.0	35.0	26.5	36.0
90-91	32.52630209736277	35.0	33.0	35.0	26.0	36.0
92-93	32.3875052809763	35.0	33.0	35.0	25.5	36.0
94-95	32.24404561062263	35.0	33.0	35.0	25.0	35.5
96-97	32.11749782381246	35.0	33.0	35.0	25.0	35.0
98-99	32.05183012410649	35.0	33.0	35.0	25.0	35.0
100-101	30.868707131940763	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	80.0
8	309.0
9	271.0
10	313.0
11	678.0
12	1188.0
13	1461.0
14	1624.0
15	1623.0
16	1768.0
17	1914.0
18	2200.0
19	2384.0
20	2903.0
21	3535.0
22	4198.0
23	3964.0
24	3627.0
25	3991.0
26	4332.0
27	4632.0
28	5268.0
29	6062.0
30	7387.0
31	8983.0
32	11604.0
33	15940.0
34	25419.0
35	43524.0
36	89623.0
37	169070.0
38	217117.0
39	58353.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49172199535409	19.559093310009143	13.7169234788634	24.232261215773363
2	15.314689478593971	20.517408082658267	39.623342340528694	24.54456009821907
3	18.92406866838399	26.39938981599493	28.965026419096034	25.71151509652504
4	12.5269010805799	17.622865989378504	39.53501889809771	30.31521403194388
5	13.784127866258745	38.32230826157349	34.499732052478016	13.393831819689748
6	29.353863689850034	37.907627572792414	19.455400205851745	13.283108531505809
7	26.667299911251245	31.744267482512527	23.186534006651904	18.401898599584328
8	25.383136602198586	34.74768983869276	21.34209101142392	18.527082547684735
9	26.16613880532266	16.09939293582586	20.79868209515114	36.93578616370034
10-11	25.07676909161537	25.76159475560067	28.584329748412873	20.57730640437109
12-13	25.998565275702408	23.20149086568315	28.437596581613413	22.362347277001028
14-15	23.12684549861437	24.955608755712007	25.747559899814775	26.16998584585885
16-17	23.025432319639517	27.44570865518922	26.631542432482092	22.897316592689172
18-19	22.831888776702552	27.161588029087774	27.44399979579566	22.562523398414015
20-21	24.060493756245	25.741474716142992	27.17432234923188	23.023709178380134
22-23	23.707383300609457	26.461650645208884	26.59011076937045	23.240855284811204
24-25	23.1792464869617	26.244274917402834	27.14915701260582	23.427321583029652
26-27	23.151658297441596	26.852594837827954	27.182779905920647	22.812966958809803
28-29	22.94396352511193	26.98458380235964	27.015348147476047	23.056104525052383
30-31	23.276204275251573	26.301813820421287	27.198374735242332	23.22360716908481
32-33	23.210918648863988	26.404597922768737	27.25557940461777	23.12890402374951
34-35	23.54202522959842	26.313439056824723	26.969839600091866	23.17469611348499
36-37	23.188473616331265	26.267318680742118	27.399267866752602	23.144939836174018
38-39	23.166080644246584	26.502155050189984	26.930613055067205	23.40115125049623
40-41	23.72636366600834	26.155534414729136	27.026057565115558	23.092044354146964
42-43	23.551353638921398	26.389561338901334	27.015501045950213	23.04358397622705
44-45	23.54054197756234	26.294170816865464	26.834730517696688	23.33055668787551
46-47	23.464192854165663	26.216538458266818	26.4637873886033	23.85548129896422
48-49	23.206594628006613	26.363838709768885	26.84798727461927	23.58157938760523
50-51	23.244447531905603	26.581173922042865	26.565224664782054	23.609153881269478
52-53	23.548192275739265	26.585994142015025	26.15302497157488	23.71278861067083
54-55	23.484182589932544	26.061795220043045	26.68360359645118	23.77041859357323
56-57	23.388416160779855	26.629730549703556	26.30138850689433	23.680464782622256
58-59	23.71115528809887	26.471263846271647	26.592143995553315	23.225436870076173
60-61	23.51226685043546	26.58061012373198	26.523536868613824	23.383586157218737
62-63	23.389695183692456	26.47551003621945	26.694259061244054	23.440535718844043
64-65	24.019381175409283	26.074588589730137	26.257563679586504	23.64846655527408
66-67	23.314748538078035	26.342245716327582	26.182582851697873	24.16042289389651
68-69	23.423433641165815	26.43500783994601	26.819136840374163	23.32242167851401
70-71	23.727887240877735	26.016003130307556	26.577346100300268	23.678763528514438
72-73	23.492972402822947	26.21171823829466	26.712737573047598	23.58257178583479
74-75	23.511686198008967	26.062149647982167	26.662337920103436	23.763826233905426
76-77	23.487230670209055	25.89911279598277	26.325560492342937	24.288096041465234
78-79	23.471683987085193	25.67916884678484	26.665858427734225	24.183288738395746
80-81	23.371497229559314	26.409406731333924	26.339259116352835	23.879836922753928
82-83	23.365936239580652	26.51707769590999	26.526297573682783	23.590688490826576
84-85	23.4241050346102	26.05564292997551	26.823080272116563	23.697171763297735
86-87	23.31677623949459	26.552112288446654	26.43337785283597	23.69773361922278
88-89	23.514866755493973	26.195286290788644	26.265913218226565	24.02393373549081
90-91	23.362401150047777	26.19023990518344	26.435858466999925	24.011500477768863
92-93	23.33776854975512	26.091123494430697	26.77065441535601	23.80045354045818
94-95	23.430593085536223	25.97864642552335	26.730458970004058	23.860301518936375
96-97	23.460081490071765	26.36915512885582	26.656596187489544	23.514167193582868
98-99	22.873404091678204	26.547493225076323	26.636596851104795	23.94250583214068
100-101	23.458100808261758	26.05330400131058	26.398962315868157	24.089632874559506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	2.5
3	4.0
4	3.0
5	2.5
6	4.5
7	9.0
8	19.0
9	25.0
10	32.5
11	37.0
12	36.0
13	45.0
14	56.5
15	62.5
16	77.5
17	107.0
18	113.5
19	125.0
20	158.0
21	189.5
22	226.5
23	297.0
24	417.0
25	592.0
26	837.0
27	1147.5
28	1567.5
29	2192.5
30	3267.0
31	4872.5
32	6981.5
33	9560.5
34	12269.0
35	14963.0
36	18127.5
37	20552.0
38	21684.5
39	22618.0
40	23079.0
41	23090.5
42	23443.5
43	24092.0
44	25002.0
45	26570.0
46	28061.5
47	28010.5
48	28475.5
49	30220.0
50	31322.5
51	30295.5
52	28661.0
53	28441.5
54	28314.5
55	27164.5
56	23229.5
57	19606.0
58	18151.0
59	16354.0
60	13297.0
61	10182.5
62	8031.0
63	5723.0
64	3608.5
65	2423.0
66	1854.5
67	1497.0
68	1149.5
69	817.5
70	566.5
71	368.5
72	235.0
73	193.5
74	152.0
75	113.5
76	86.0
77	54.5
78	31.5
79	21.5
80	16.0
81	11.5
82	9.5
83	9.5
84	5.5
85	3.0
86	4.5
87	2.5
88	0.5
89	2.0
90	3.5
91	4.0
92	3.5
93	2.0
94	1.5
95	2.5
96	2.5
97	1.5
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15396349675769322
2	0.0
3	1.4177117565165122E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03799467507464253
16-17	0.07520960868320097
18-19	0.026369438671207123
20-21	0.04217692475636623
22-23	0.06719953725888267
24-25	0.01871379518601796
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023037816043393323
38-39	0.00595438937736935
40-41	0.0055290758504143965
42-43	0.003189851452162152
44-45	0.010561952586048015
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.01672899872689484
60-61	0.018146710483411356
62-63	0.03062257394075666
64-65	0.04933636912677462
66-67	0.017863168132108052
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0177213969564564
80-81	0.059543893773693506
82-83	0.05153382234937521
84-85	0.031614972170318216
86-87	0.06060717759108089
88-89	0.03523013714943533
90-91	0.0
92-93	0.003189851452162152
94-95	0.0
96-97	0.0
98-99	4.961991147807793E-4
100-101	0.04685537355287073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	705362.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.95427512958348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00604029309048	53.426946686486154
2	12.533059929421498	16.532177656012472
3	3.343449839589322	6.615444318067077
4	1.2565190400609976	3.3149120989497267
5	0.5806062457879058	1.9146732038325052
6	0.3144915460688867	1.2445237173213257
7	0.19387240824826707	0.8950699907548796
8	0.12154144586453199	0.6412942368157375
9	0.07722395787595783	0.45839251479116594
>10	0.4707877621588832	5.9179473595243035
>50	0.05499293337488441	2.5411913525172243
>100	0.045033043285318486	5.363354425170193
>500	0.0021650501573060353	0.9899929771893029
>1k	2.1650501573060353E-4	0.14407946256800902
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1009	0.14304711623251606	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	941	0.13340667628820377	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	939	0.13312313393690048	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	909	0.12886999866735097	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	827	0.11724476226391556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	2.8354235130330244E-4	0.0
30-31	0.0	0.0	0.0	0.0015594829321681635	0.0
32-33	0.0	0.0	0.0	0.006025274965195176	0.0
34-35	0.0	0.0	0.0	0.01176700757908705	0.0
36-37	0.0	0.0	0.0	0.020627706057315252	0.0
38-39	0.0	0.0	0.0	0.03820733183812	0.0
40-41	0.0	0.0	0.0	0.05890592348326108	0.0
42-43	0.0	0.0	0.0	0.08017159983100876	0.0
44-45	0.0	0.0	0.0	0.1027132167596213	0.0
46-47	0.0	0.0	0.0	0.13432818892993953	0.0
48-49	0.0	0.0	0.0	0.1680697287350325	0.0
50-51	0.0	0.0	0.0	0.20329986588446783	0.0
52-53	0.0	0.0	0.0	0.24008948596607133	0.0
54-55	0.0	0.0	0.0	0.27737530516245557	0.0
56-57	0.0	0.0	0.0	0.31877248845273776	0.0
58-59	0.0	0.0	0.0	0.36725823052560247	0.0
60-61	0.0	0.0	0.0	0.42219456109061726	0.0
62-63	0.0	0.0	0.0	0.48769284424168013	0.0
64-65	0.0	0.0	0.0	0.5474493947788512	0.0
66-67	0.0	0.0	0.0	0.6146489320377339	0.0
68-69	0.0	0.0	0.0	0.6968762139156914	0.0
70-71	0.0	0.0	0.0	0.7909413889605621	0.0
72-73	0.0	0.0	0.0	0.8908900677949763	0.0
74-75	0.0	0.0	0.0	0.9936032845545975	0.0
76-77	0.0	0.0	0.0	1.0948987895577023	0.0
78-79	0.0	0.0	0.0	1.2146954329833477	0.0
80-81	0.0	0.0	0.0	1.3583096339184701	0.0
82-83	0.0	0.0	0.0	1.52014143092483	0.0
84-85	0.0	0.0	0.0	1.673396071804265	0.0
86-87	0.0	1.4177117565165122E-4	0.0	1.8357240679254057	0.0
88-89	0.0	1.4177117565165122E-4	0.0	2.028745523575129	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	170	0.0	53.1052	9
ACCGTGC	200	0.0	47.484863	8
AAACCGT	225	0.0	46.426357	6
GTATCAA	970	0.0	43.633545	1
GGTATCA	525	0.0	43.479465	1
AACCGTG	220	0.0	43.164997	7
ATCAACG	1195	0.0	34.170788	3
TCAACGC	1225	0.0	33.33395	4
TATCAAC	1495	0.0	27.948975	2
GTAATCG	35	0.008373336	27.155384	14-15
ATGCGGG	215	5.820766E-11	26.50318	8
ACGCAGA	1530	0.0	26.378609	7
CGCAGAG	1560	0.0	25.873163	8
TACATGG	2190	0.0	25.58364	2
CAACGCA	1630	0.0	25.051588	5
AATGCGG	190	1.1943484E-8	24.990261	7
CGGCTTA	200	0.0	24.949009	16-17
GTACATG	2235	0.0	24.68213	1
TGCGCGG	185	0.0	24.403149	12-13
AACGCAG	1700	0.0	23.74075	6
>>END_MODULE