FastQCFastQC Report
Fri 10 Feb 2017
SRR3224486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705362
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC8710.1234826939925882No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT7990.11327516934566931No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG7450.10561952586048014No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT7410.10505244115787354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.056.8148161
CCGTGCG1700.053.077229
GTATCAA12850.044.5413741
ACCGTGC2050.044.0152558
AACCGTG2450.038.7674677
AAACCGT2450.038.7674676
ATCAACG16250.034.4851533
TCAACGC16600.033.7580574
CAACGCA19150.029.2628575
TATCAAC19600.029.07562
CGCAGAG19750.028.6143158
AACGCAG19950.028.5655026
GTGCGCG1850.028.23738510-11
AATGCGG1354.2288775E-728.1423077
ACGCAGA20300.028.0729927
GTCTAAT4200.027.255081
CGGCTTA1700.026.5386116-17
GCGCGGC1900.026.24455512-13
GTATAGG2006.8394E-1026.2330151
ATACCGT1151.2828728E-424.7774686
Produced by FastQC (version 0.11.5)