##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224486_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 705362 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1499953215512 34.0 33.0 34.0 31.0 34.0 2 33.285714285714285 34.0 34.0 34.0 31.0 34.0 3 33.405254323312 34.0 34.0 34.0 31.0 34.0 4 36.619687479620396 37.0 37.0 37.0 35.0 37.0 5 36.65186528335805 37.0 37.0 37.0 35.0 37.0 6 36.68577411315041 37.0 37.0 37.0 36.0 37.0 7 36.65863485699541 37.0 37.0 37.0 35.0 37.0 8 36.64303293911495 37.0 37.0 37.0 35.0 37.0 9 38.48002160592717 39.0 39.0 39.0 37.0 39.0 10-11 38.51007993058883 39.0 39.0 39.0 37.0 39.0 12-13 38.50783214859888 39.0 39.0 39.0 37.0 39.0 14-15 40.113263827651615 41.0 40.0 41.0 38.0 41.0 16-17 40.12091663571329 41.0 40.0 41.0 38.0 41.0 18-19 40.11400599975615 41.0 40.0 41.0 38.0 41.0 20-21 40.11548963510935 41.0 40.0 41.0 38.0 41.0 22-23 40.04287727436409 41.0 40.0 41.0 38.0 41.0 24-25 40.01096316501314 41.0 40.0 41.0 38.0 41.0 26-27 39.94807276263819 41.0 40.0 41.0 38.0 41.0 28-29 39.916380525177146 41.0 40.0 41.0 38.0 41.0 30-31 39.850204575806465 41.0 40.0 41.0 38.0 41.0 32-33 39.79845597012598 41.0 40.0 41.0 38.0 41.0 34-35 39.714399840082116 41.0 40.0 41.0 37.0 41.0 36-37 39.61799827606251 41.0 40.0 41.0 37.0 41.0 38-39 39.52024279731542 41.0 40.0 41.0 37.0 41.0 40-41 39.39310169813514 41.0 39.5 41.0 36.0 41.0 42-43 39.303618567487334 41.0 39.0 41.0 36.0 41.0 44-45 39.08188561334464 41.0 39.0 41.0 35.0 41.0 46-47 38.8666876015436 41.0 39.0 41.0 35.0 41.0 48-49 38.793803040141086 40.0 39.0 41.0 35.0 41.0 50-51 38.666435107079764 40.0 38.0 41.0 35.0 41.0 52-53 38.4607683714178 40.0 38.0 41.0 35.0 41.0 54-55 38.2956219643247 40.0 37.0 41.0 35.0 41.0 56-57 38.09611376853304 40.0 37.0 41.0 34.5 41.0 58-59 37.85191575389658 40.0 36.0 41.0 34.0 41.0 60-61 37.61333967523059 39.5 35.5 41.0 34.0 41.0 62-63 37.38925899041911 39.0 35.0 41.0 34.0 41.0 64-65 37.11533368681613 39.0 35.0 41.0 33.5 41.0 66-67 36.80883999988659 38.0 35.0 40.5 33.0 41.0 68-69 36.52237999778836 37.0 35.0 40.0 33.0 41.0 70-71 36.19669687337849 37.0 35.0 39.5 33.0 41.0 72-73 35.85350997076678 36.0 35.0 39.0 32.0 41.0 74-75 35.50844601778945 36.0 35.0 39.0 32.0 40.5 76-77 33.64428477859596 34.5 32.5 36.5 29.5 39.0 78-79 34.897021671141914 35.0 34.5 37.0 32.0 39.0 80-81 34.79447007352253 35.0 35.0 37.0 32.0 39.0 82-83 34.56123947703449 35.0 35.0 36.0 32.0 38.0 84-85 34.330409066550224 35.0 35.0 36.0 32.0 37.0 86-87 34.11749073525367 35.0 35.0 36.0 32.0 37.0 88-89 33.94544148961809 35.0 35.0 35.0 32.0 36.5 90-91 33.799322900865086 35.0 34.0 35.0 31.5 36.0 92-93 33.69516787124908 35.0 34.0 35.0 31.0 36.0 94-95 33.59492785264871 35.0 34.0 35.0 31.0 36.0 96-97 33.544172354053664 35.0 34.0 35.0 31.0 35.5 98-99 33.49383082729152 35.0 34.0 35.0 31.0 35.0 100-101 32.67818084898251 34.5 33.0 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 22.0 15 44.0 16 118.0 17 265.0 18 473.0 19 728.0 20 1040.0 21 1569.0 22 2140.0 23 2025.0 24 1770.0 25 1873.0 26 2382.0 27 3025.0 28 3879.0 29 4537.0 30 5120.0 31 5966.0 32 7564.0 33 9907.0 34 16379.0 35 31300.0 36 79198.0 37 178916.0 38 262912.0 39 82172.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83343542114785 19.60983461774049 13.803507072548824 23.753222888562842 2 15.257555694806355 20.471615992922786 40.058012765076654 24.212815547194207 3 18.730665955920507 26.708413552190223 29.19437111724193 25.366549374647345 4 12.570850145031912 17.706510983012976 39.89880373481985 29.82383513713526 5 13.825666820724678 37.78060059940853 34.99749065019097 13.396241929675826 6 29.30140835485892 37.61628780682827 19.78005619809403 13.30224764021878 7 26.595847238722815 31.707265205667447 23.365449230324288 18.331438325285458 8 25.38101003456381 34.72982667056065 21.21761591920177 18.671547375673768 9 26.10872147918374 16.295462471752092 20.864322149477857 36.73149389958631 10-11 25.164950762870696 25.701554662712194 28.69845554481245 20.435039029604656 12-13 26.166705890025266 23.361125209466913 28.40569806709179 22.066470833416034 14-15 23.15024058568508 25.100090450010065 26.007284203004982 25.74238476129987 16-17 22.96600894292576 27.51494977047247 26.725638750031898 22.793402536569875 18-19 22.658507262937327 27.187103926778022 27.928212747497028 22.226176062787616 20-21 23.941034198236924 25.90890016133651 27.58081711698173 22.56924852344483 22-23 23.542859330208664 26.47769772341264 27.004383614468765 22.975059331909932 24-25 23.357684930127977 26.14845447933753 27.343374527369672 23.15048606316482 26-27 23.13447224782023 26.93464237612533 27.264336854044092 22.66654852201035 28-29 22.98312235987816 26.869821214262878 27.42735256609741 22.719703859761548 30-31 23.3519669500224 26.095687851241074 27.487396435275237 23.064948763461288 32-33 23.15924305533896 26.399423274857448 27.627232541588576 22.814101128215018 34-35 23.558613512841035 26.403922099593597 27.171853434495453 22.86561095306992 36-37 23.137907617363886 26.25572588888621 27.39190103055367 23.214465463196234 38-39 23.121461037027796 26.58230809144808 27.050011199922874 23.24621967160125 40-41 23.515726676515037 26.3888613222714 27.20936200135533 22.88604999985823 42-43 23.257703136829033 26.429974963210377 27.415993489867613 22.896328410092973 44-45 23.601604708354394 26.316054385406485 27.126348451969694 22.955992454269428 46-47 23.386538406969155 26.105388609857837 26.814860531766975 23.693212451406033 48-49 23.19013890818563 26.107451818792725 27.179177098721503 23.523232174300144 50-51 23.503959668935952 26.26339383181969 26.78773452496732 23.444911974277037 52-53 23.528131654384556 26.55714370776991 26.524252795018725 23.390471842826805 54-55 23.58441481111826 26.040671314870945 26.89051862731477 23.48439524669602 56-57 23.55005373584649 26.394650802067765 26.47929539402979 23.57600006805595 58-59 23.737865489092858 26.40513540940523 26.742976361770644 23.114022739731276 60-61 23.614217258059714 26.54567938701795 26.650764454869435 23.189338900052896 62-63 23.485174988162107 26.22674598291374 26.852169524300994 23.435909504623158 64-65 23.64020176873719 26.203211967755564 26.592232073743695 23.564354189763552 66-67 23.29746995159932 26.326482004984676 26.609031958058416 23.76701608535759 68-69 23.557549173332276 26.305287214224755 26.829557021784563 23.307606590658413 70-71 23.78587165171926 25.976448972300748 26.73783107113794 23.499848304842054 72-73 23.410532464181514 26.108863250359388 26.89590593198953 23.584698353469566 74-75 23.622763914132033 25.977086942591182 26.697071858138088 23.703077285138693 76-77 23.526834859621427 25.79617089417397 26.579387419624435 24.09760682658017 78-79 23.577880128141963 25.75039539267402 26.656023615097723 24.015700864086295 80-81 23.291113756086773 26.131311935495994 26.66787651854724 23.909697789869995 82-83 23.3427776899581 26.325653797815157 26.76025487712294 23.571313635103802 84-85 23.554614176264764 25.926209473905082 26.930252868087813 23.588923481742345 86-87 23.16058988150765 26.496820072530134 26.68707699025465 23.655513055707566 88-89 23.57158451972179 26.048752271883092 26.332578165537697 24.04708504285743 90-91 23.53926069167321 26.261125493009263 26.37071461178799 23.828899203529534 92-93 23.579965733882556 26.16559026825252 26.707723628238856 23.54672036962607 94-95 23.38224911463901 26.09780509865856 26.569477800051605 23.950467986650825 96-97 23.48836483961427 26.444010309599893 26.62675335501487 23.440871495770963 98-99 22.629798599867872 26.629801435291384 26.834944326459322 23.905455638381426 100-101 23.18275384133046 26.32774340721153 26.540185189651282 23.949317561806726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 2.5 2 3.5 3 4.0 4 4.0 5 5.5 6 5.5 7 12.5 8 24.0 9 31.5 10 30.0 11 29.0 12 39.0 13 50.0 14 64.5 15 82.0 16 90.0 17 93.5 18 108.5 19 128.5 20 161.0 21 190.0 22 239.0 23 329.0 24 441.0 25 593.0 26 916.5 27 1322.0 28 1767.5 29 2498.5 30 3607.5 31 5134.0 32 7145.5 33 9737.5 34 12524.0 35 15334.5 36 18275.0 37 20448.0 38 21693.0 39 22562.0 40 22977.0 41 23149.5 42 23643.0 43 24441.5 44 25387.5 45 26762.0 46 27873.0 47 28260.5 48 28772.5 49 30512.0 50 32164.5 51 30759.5 52 28218.5 53 27740.0 54 28034.0 55 26643.5 56 22938.5 57 19597.0 58 18102.0 59 16667.5 60 13344.5 61 9709.5 62 7499.0 63 5217.5 64 3187.0 65 2112.0 66 1615.0 67 1292.0 68 969.0 69 684.5 70 428.5 71 270.5 72 194.0 73 147.5 74 115.0 75 80.5 76 51.0 77 35.5 78 21.5 79 6.0 80 2.5 81 3.5 82 1.5 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47521698078433483 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.670847026066049E-4 22-23 0.0011341694052132098 24-25 1.4177117565165122E-4 26-27 0.0017012541078198144 28-29 0.00290630910085885 30-31 8.506270539099072E-4 32-33 0.0 34-35 0.0036151649791171057 36-37 0.0018430252834714656 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0088606984782282 46-47 0.07683997720319495 48-49 5.670847026066049E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008506270539099072 58-59 0.042035153580714586 60-61 0.031047887467711616 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.126567634774768E-4 78-79 0.0071594443704083854 80-81 0.005883503789543525 82-83 3.54427939129128E-4 84-85 0.001984796459123117 86-87 0.0 88-89 0.0 90-91 0.0 92-93 7.088558782582561E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0656400543267145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 705362.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.29871219101108 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86532919970466 51.35237816252164 2 13.075720772681946 16.815040133054026 3 3.6670600198164176 7.073617104040177 4 1.3886321776034831 3.5714904290761336 5 0.639316082299279 2.0553600387423048 6 0.33321703660493773 1.2855255800281498 7 0.20083156286147463 0.9039247601510635 8 0.13194112910888017 0.6786915749383136 9 0.09994080775373441 0.5783458710505085 >10 0.48415686874761993 5.997099402336689 >50 0.060678506802875135 2.707101601101193 >100 0.04986613667875518 5.654345893112474 >500 0.003309699335978698 1.327079449847367 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC 871 0.1234826939925882 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 799 0.11327516934566931 No Hit TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 745 0.10561952586048014 No Hit ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT 741 0.10505244115787354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 2.8354235130330244E-4 0.0 30-31 0.0 0.0 0.0 0.0015594829321681635 0.0 32-33 0.0 0.0 0.0 0.00645058849215013 0.0 34-35 0.0 0.0 0.0 0.012404977869519482 0.0 36-37 0.0 0.0 0.0 0.021549218699050986 0.0 38-39 0.0 0.0 0.0 0.039341501243333216 0.0 40-41 0.0 0.0 0.0 0.060890719942384194 0.0 42-43 0.0 0.0 0.0 0.08215639629013188 0.0 44-45 0.0 0.0 0.0 0.10469801321874442 0.0 46-47 0.0 0.0 0.0 0.136171214213411 0.0 48-49 0.0 0.0 0.0 0.17033806754545894 0.0 50-51 0.0 0.0 0.0 0.20556820469489426 0.0 52-53 0.0 0.0 0.0 0.24313756624258182 0.0 54-55 0.0 0.0 0.0 0.2805651566146178 0.0 56-57 0.0 0.0 0.0 0.32295473813446146 0.0 58-59 0.0 0.0 0.0 0.3721493360855844 0.0 60-61 0.0 0.0 0.0 0.4278654081166834 0.0 62-63 0.0 0.0 0.0 0.49378900479470117 0.0 64-65 0.0 0.0 0.0 0.5534037841562205 0.0 66-67 0.0 0.0 0.0 0.6212412917055357 0.0 68-69 0.0 0.0 0.0 0.7046027429887065 0.0 70-71 0.0 0.0 0.0 0.8002274009657453 0.0 72-73 0.0 0.0 0.0 0.9022317618471083 0.0 74-75 0.0 0.0 0.0 1.0062209191875944 0.0 76-77 0.0 0.0 0.0 1.109288563886345 0.0 78-79 0.0 0.0 0.0 1.2309991181832873 0.0 80-81 0.0 0.0 0.0 1.3764563444018816 0.0 82-83 0.0 0.0 0.0 1.5399893955160613 0.0 84-85 0.0 0.0 0.0 1.6940946634494063 0.0 86-87 0.0 0.0 0.0 1.8586909983809732 0.0 88-89 0.0 0.0 0.0 2.0531301657872127 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 680 0.0 56.814816 1 CCGTGCG 170 0.0 53.07722 9 GTATCAA 1285 0.0 44.541374 1 ACCGTGC 205 0.0 44.015255 8 AACCGTG 245 0.0 38.767467 7 AAACCGT 245 0.0 38.767467 6 ATCAACG 1625 0.0 34.485153 3 TCAACGC 1660 0.0 33.758057 4 CAACGCA 1915 0.0 29.262857 5 TATCAAC 1960 0.0 29.0756 2 CGCAGAG 1975 0.0 28.614315 8 AACGCAG 1995 0.0 28.565502 6 GTGCGCG 185 0.0 28.237385 10-11 AATGCGG 135 4.2288775E-7 28.142307 7 ACGCAGA 2030 0.0 28.072992 7 GTCTAAT 420 0.0 27.25508 1 CGGCTTA 170 0.0 26.53861 16-17 GCGCGGC 190 0.0 26.244555 12-13 GTATAGG 200 6.8394E-10 26.233015 1 ATACCGT 115 1.2828728E-4 24.777468 6 >>END_MODULE