FastQCFastQC Report
Fri 10 Feb 2017
SRR3224485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845143
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT27150.3212474102015872No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26890.318171007746618No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24920.2948613429916594No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG24500.2898917697951708No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA22830.27013180018056115No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA22770.26942186115249134No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG22270.263505702585243No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG20320.24043268417297428No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT19900.23546311097648565No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT19310.22848204386713253No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18940.22410408652736874No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG18720.22150097675777947No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG18380.21747798893205056No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG18250.21593978770456596No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA17730.20978698279462768No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA16660.19712640346071614No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC16600.19641646443264632No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC16570.1960614949186114No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC15570.18422917778411463No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT15110.17878631190224614No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14660.1734617691917226No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA14240.16849219599523393No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT13960.16517914719757484No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13720.16233939108529563No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC13380.15831640325956672No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG12970.15346515323442306No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA12910.15275521420635324No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG12700.15027042760810894No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT12530.14825893369524448No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG12400.1467207324677599No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA12030.1423427751279961No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC11930.14115954341454642No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG11460.13559835436133294No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT11040.13062878116484428No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC10660.12613250065373552No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC10650.12601417748239055No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC10320.12210951282800662No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC10110.1196247262297623No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC10020.11855981768765758No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT9990.11820484817362269No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC9950.11773155548824282No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA9940.11761323231689784No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT9800.11595670791806831No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA9720.11501012254730857No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG9690.11465515303327364No Hit
CCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAAAGTGCGGCAAT9660.11430018351923875No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9550.11299862863444411No Hit
CCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGACGAACAGTGGATT9430.1115787505783045No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC9390.11110545789292463No Hit
GTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGA9360.11075048837888973No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA9080.10743743958123063No Hit
CCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGC8980.10625420786778096No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC8950.10589923835374607No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG8810.10424271395491651No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT8670.10258618955608695No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG8640.10223122004205205No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT8560.10128463467129231No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG8520.10081134198591243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT353.3147626E-668.202581
GGTATCA6800.054.7626651
GTATCAA16450.053.401171
CCGTGCG1850.048.773949
ACCGTGC2150.046.3859868
AACCGTG2050.046.332117
ATCAACG23400.036.1251873
GTATTAC1203.607056E-935.8063551
CTATTAA4300.035.5287861
CCTTGGC3400.034.9194229
TCAACGC25800.032.7647064
ACCCTTG4000.032.056037
AAACCGT3000.031.6602766
AACCCTT4050.030.4876756
CCCTTGG3900.030.4425748
CGCAGAG27900.029.9581138
CATGGGA11300.029.8391114
GTACATG48950.029.7471941
CATGGGT4000.029.681514
TCCGGGC10600.028.6734568
Produced by FastQC (version 0.11.5)