FastQCFastQC Report
Fri 10 Feb 2017
SRR3224484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621170
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG6960.1120466216977639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.044.5896381
CCGTGCG652.5885001E-643.843559
TAGCGTG702.0549071E-433.92115
ACCGTAT804.4970884E-429.680968
GGACCGC650.00584832629.2243296
AGGACCG856.412095E-427.935025
ATCAACG7650.027.3142414
ACCGTGC1405.810052E-727.1368798
GTATCAA7750.026.96182
TCAACGC7750.026.96185
AAACCGT950.001227495524.9944936
GTACTAC1151.2694809E-424.8151021
CAACGCA8550.024.439066
TCGCACC2602.3646862E-1123.7811371
GTACATG17850.023.1816141
GTATATA4150.022.921581
ACATGGG15750.022.9155853
CGCACCA2704.0017767E-1122.8653322
ACGCAGA9000.022.6894458
AACGCAG9000.022.6894457
Produced by FastQC (version 0.11.5)