##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224484_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621170
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.406508685223045	34.0	31.0	34.0	31.0	34.0
2	32.564629650498254	34.0	31.0	34.0	31.0	34.0
3	32.71128515543249	34.0	33.0	34.0	31.0	34.0
4	35.98051419096222	37.0	37.0	37.0	35.0	37.0
5	36.00245504451277	37.0	37.0	37.0	35.0	37.0
6	36.110431926847724	37.0	37.0	37.0	35.0	37.0
7	36.06561005843811	37.0	37.0	37.0	35.0	37.0
8	36.10272389201024	37.0	37.0	37.0	35.0	37.0
9	37.85271020815558	39.0	39.0	39.0	37.0	39.0
10-11	37.94245858621633	39.0	39.0	39.0	37.0	39.0
12-13	37.969274111756846	39.0	39.0	39.0	37.0	39.0
14-15	39.52717130576171	41.0	40.0	41.0	38.0	41.0
16-17	39.37362235780865	41.0	40.0	41.0	37.5	41.0
18-19	39.46560039924658	41.0	40.0	41.0	37.5	41.0
20-21	39.53299579825169	41.0	40.0	41.0	38.0	41.0
22-23	39.50998277444178	41.0	40.0	41.0	38.0	41.0
24-25	39.4823792198593	41.0	40.0	41.0	37.5	41.0
26-27	39.359278458393035	41.0	40.0	41.0	37.0	41.0
28-29	39.28852568540013	41.0	40.0	41.0	37.0	41.0
30-31	39.2365849928361	41.0	40.0	41.0	37.0	41.0
32-33	39.06871387864835	41.0	40.0	41.0	36.0	41.0
34-35	39.0976866236296	41.0	40.0	41.0	36.0	41.0
36-37	39.11905677994752	41.0	40.0	41.0	36.0	41.0
38-39	39.057512436209095	41.0	39.5	41.0	36.0	41.0
40-41	39.00432168327511	41.0	39.0	41.0	35.5	41.0
42-43	38.90833910201716	41.0	39.0	41.0	35.0	41.0
44-45	38.80919555033243	41.0	39.0	41.0	35.0	41.0
46-47	38.724690503404865	41.0	39.0	41.0	35.0	41.0
48-49	38.59033436901331	40.5	39.0	41.0	35.0	41.0
50-51	37.421218024051385	39.0	37.5	40.0	33.0	40.5
52-53	37.79361527440153	39.5	38.0	40.5	33.5	41.0
54-55	38.354258093597565	40.0	38.0	41.0	34.0	41.0
56-57	38.30777886890867	40.0	38.0	41.0	34.0	41.0
58-59	38.240228117906526	40.0	38.0	41.0	34.0	41.0
60-61	38.0810357873046	40.0	37.0	41.0	34.0	41.0
62-63	37.85551620329379	40.0	37.0	41.0	33.5	41.0
64-65	37.671789526216656	40.0	36.0	41.0	33.0	41.0
66-67	37.46220921808845	39.5	36.0	41.0	33.0	41.0
68-69	37.1885160262086	39.0	35.0	41.0	33.0	41.0
70-71	36.90498655762512	39.0	35.0	41.0	32.5	41.0
72-73	36.58856351723361	38.5	35.0	40.5	32.0	41.0
74-75	36.265760580839384	37.0	35.0	40.0	32.0	41.0
76-77	35.84570165977108	37.0	35.0	39.0	31.5	41.0
78-79	35.49566704766811	36.0	35.0	39.0	31.0	40.5
80-81	35.07937601622744	36.0	35.0	38.0	31.0	39.0
82-83	34.709220503243884	35.5	35.0	37.0	31.0	39.0
84-85	34.37527488449217	35.0	35.0	37.0	31.0	39.0
86-87	34.05067211874366	35.0	34.0	36.5	30.0	37.0
88-89	33.80311750406491	35.0	34.0	36.0	30.0	37.0
90-91	33.56726982951527	35.0	34.0	36.0	30.0	37.0
92-93	33.37362557753916	35.0	34.0	35.0	29.5	36.0
94-95	33.20656422557432	35.0	34.0	35.0	29.0	36.0
96-97	33.05556369431879	35.0	34.0	35.0	29.0	36.0
98-99	32.968889353961075	35.0	34.0	35.0	29.0	36.0
100-101	31.891709193940468	34.5	32.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	9.0
7	96.0
8	373.0
9	278.0
10	230.0
11	435.0
12	683.0
13	829.0
14	887.0
15	929.0
16	965.0
17	1021.0
18	1108.0
19	1296.0
20	1476.0
21	1776.0
22	2000.0
23	2069.0
24	2039.0
25	2248.0
26	2420.0
27	2724.0
28	3101.0
29	3578.0
30	4474.0
31	5580.0
32	7189.0
33	10196.0
34	15587.0
35	26392.0
36	53204.0
37	111717.0
38	239685.0
39	114563.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71732155237742	21.24973799197046	14.226794150368423	22.806146305283693
2	14.199494502310156	21.293687718337974	42.46486469082538	22.04195308852649
3	18.14884516130071	26.988146543050277	30.799347681548824	24.063660614100186
4	11.536938358259412	17.615950544939388	41.473348680715425	29.373762416085775
5	12.087351288697135	39.2969718434567	36.80377352415603	11.811903343690133
6	28.260701579277814	39.85994172287779	19.601558349566144	12.27779834827825
7	25.192137418098103	33.1691807395721	24.30220390553311	17.33647793679669
8	23.201378044657663	38.2085419450392	21.770207833604328	16.819872176698812
9	24.25616175926075	17.77468325901122	21.825748184876925	36.1434067968511
10-11	23.155577378173447	27.496498543071944	30.534636250945795	18.81328782780881
12-13	23.619701530981857	24.917816378769096	30.476600608529065	20.985881481719982
14-15	20.918601926550174	27.30481863002845	27.22872609828901	24.547853345132374
16-17	20.95579232930381	30.39410263680181	27.940063020336623	20.71004201355775
18-19	20.986719956457815	29.688634749688813	29.006597349144215	20.318047944709157
20-21	21.52158350720014	28.596184244325606	29.192713135472776	20.689519113001477
22-23	21.25329714361653	28.7177483982426	28.924335839498067	21.104618618642807
24-25	21.049296891541687	28.666633927126618	29.033021583139245	21.251047598192454
26-27	21.05092003799282	29.05267479111998	28.968881304634802	20.927523866252397
28-29	20.984915562567412	29.00486179306792	29.069175909976337	20.941046734388333
30-31	21.27549624096463	28.532366340937266	28.934752161244106	21.257385256854
32-33	20.78851200154547	28.82544231047861	29.026433987475247	21.35961170050067
34-35	21.299885699566946	28.840494550606117	28.672344124796755	21.187275625030185
36-37	21.12013474530352	28.62902933624895	29.01355584772557	21.237280070721962
38-39	21.052661235792254	28.688057442766524	28.937357117558037	21.32192420388318
40-41	21.28462847492228	28.621993176940702	29.104984632821846	20.988393715315176
42-43	21.294400900273608	28.591817201955422	28.916057374733256	21.197724523037714
44-45	21.441147276112822	28.535949385635057	28.80660324575322	21.216300092498894
46-47	21.454191284189513	28.357937440636217	28.665341210940642	21.522530064233624
48-49	21.10734581515527	28.52858315758971	28.947711576541042	21.416359450713976
50-51	21.18960992964889	28.678864079076583	28.702851071365327	21.428674919909206
52-53	21.240964631260365	28.57221050598065	28.646827760516448	21.53999710224254
54-55	21.098974515833024	28.51948741890304	28.884282885522484	21.497255179741455
56-57	21.131091327655877	28.637893008355203	28.644815428948593	21.586200235040327
58-59	21.274165124290434	28.640135380245724	28.727243744822406	21.35845575064144
60-61	21.383699951453927	28.512324503529857	28.69918260649745	21.404792938518774
62-63	21.176245142859905	28.472945749791062	28.830679250812814	21.520129856536226
64-65	21.370193294952973	28.385923121709865	28.545210467868326	21.698673115468832
66-67	21.262570273170724	28.61348810136243	28.299588525074533	21.82435310039231
68-69	21.294573144227826	28.538805801954375	28.855627283996327	21.310993769821465
70-71	21.319284575881	28.192926252072702	28.929842072218552	21.557947099827746
72-73	21.22486597871758	28.417341468519087	28.80950464446126	21.548287908302076
74-75	21.127227651045608	28.309078030168877	28.787449490477645	21.77624482830787
76-77	21.126422718418468	28.279215029702016	28.793405991918476	21.80095625996104
78-79	21.352952352736594	28.019495575335227	28.702677984877567	21.92487408705061
80-81	21.215425651862972	28.38342170879266	28.44615969434762	21.954992944996746
82-83	21.126262630329865	28.55310194539494	28.58821359803439	21.732421826240806
84-85	21.357762264789002	28.110260312245867	28.712126701933222	21.81985072103191
86-87	20.95081241065496	28.481849115106613	28.798115448889604	21.769223025348825
88-89	21.314989166291586	28.13495153213839	28.446395487122338	22.103663814447682
90-91	21.28241866155803	28.012943316644396	28.658177310559108	22.04646071123847
92-93	21.405376861688854	27.947912155493988	28.84070173856906	21.806009244248102
94-95	21.564789027158426	27.797382359096545	28.59032149009128	22.04750712365375
96-97	21.514239258174094	27.886971360497125	28.825925270054896	21.772864111273886
98-99	21.18472642915041	27.889472112459746	28.758646059135707	22.167155399254142
100-101	21.568194812410916	27.81117884378447	28.396292508515796	22.22433383528881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	2.5
4	3.5
5	4.5
6	5.0
7	8.0
8	15.0
9	18.0
10	15.5
11	16.5
12	20.5
13	22.0
14	25.0
15	31.0
16	39.0
17	43.5
18	49.0
19	54.0
20	68.5
21	82.0
22	89.5
23	101.0
24	145.5
25	193.5
26	242.5
27	346.0
28	536.0
29	1052.0
30	2163.0
31	4467.0
32	8709.5
33	15111.5
34	22841.0
35	30300.0
36	37270.5
37	42627.0
38	45301.5
39	45446.5
40	40919.0
41	33715.5
42	28048.5
43	23650.5
44	19857.0
45	16902.0
46	15058.0
47	13560.5
48	12635.5
49	12759.0
50	13046.5
51	12904.0
52	12722.0
53	12844.5
54	13034.5
55	12842.5
56	11488.5
57	10028.5
58	9238.0
59	8600.5
60	7266.5
61	5602.5
62	4547.0
63	3444.0
64	2272.5
65	1572.5
66	1227.0
67	1005.0
68	790.5
69	569.0
70	389.0
71	284.0
72	221.5
73	162.5
74	115.5
75	90.0
76	68.5
77	46.5
78	36.5
79	20.0
80	14.5
81	14.0
82	14.5
83	13.5
84	9.0
85	7.0
86	5.5
87	3.5
88	2.5
89	3.0
90	2.0
91	1.5
92	2.0
93	1.5
94	2.5
95	3.5
96	5.0
97	5.5
98	4.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1545470644107088
2	0.0
3	1.6098652542782167E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.034692596229695574
16-17	0.06729236762882947
18-19	0.026240803644734936
20-21	0.03936120546710241
22-23	0.059162548094724474
24-25	0.017145064958063012
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023584525975175876
38-39	0.00482959576283465
40-41	0.006600447542540689
42-43	0.004105156398409453
44-45	0.013120401822367468
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016823091907207367
60-61	0.017789011059774297
62-63	0.028494615000724435
64-65	0.04499573385707616
66-67	0.017949997585202118
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0170645716953491
80-81	0.05376949949289244
82-83	0.047249545213065666
84-85	0.02978250720414701
86-87	0.05352801970475072
88-89	0.030828919619427856
90-91	0.0
92-93	0.004346636186551186
94-95	0.0
96-97	0.0
98-99	8.854258898530193E-4
100-101	0.042661429238372746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	621170.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.32976306637725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89616557491664	39.60431404132627
2	13.65764490023057	14.840332229484849
3	5.316106415799087	8.66468406018037
4	2.6693541280222584	5.801015092628213
5	1.581008906933356	4.294791965976065
6	1.017640944409444	3.317291483784569
7	0.6513016195171273	2.4769543871179316
8	0.4594901105466293	1.9971191069873488
9	0.33336282689769564	1.6300371060440584
>10	1.3097941741498318	11.834808764611498
>50	0.0766797466792568	2.837593674128215
>100	0.031151121880101055	2.587795259133251
>500	2.995300180771892E-4	0.11326282859727364
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	696	0.1120466216977639	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	1.609865254278217E-4	0.0
20-21	0.0	0.0	0.0	1.609865254278217E-4	1.609865254278217E-4
22-23	0.0	0.0	0.0	4.024663135695542E-4	1.609865254278217E-4
24-25	0.0	0.0	0.0	8.049326271391084E-4	1.609865254278217E-4
26-27	0.0	0.0	0.0	0.0016903585169921278	1.609865254278217E-4
28-29	0.0	0.0	0.0	0.0033807170339842556	1.609865254278217E-4
30-31	0.0	0.0	0.0	0.008934752161244104	1.609865254278217E-4
32-33	0.0	0.0	0.0	0.023423539449748055	1.609865254278217E-4
34-35	0.0	0.0	0.0	0.04290290902651448	1.609865254278217E-4
36-37	0.0	0.0	0.0	0.07501972084936491	1.609865254278217E-4
38-39	0.0	0.0	0.0	0.1261329426726983	1.609865254278217E-4
40-41	0.0	0.0	0.0	0.18650288970813142	1.609865254278217E-4
42-43	0.0	0.0	0.0	0.2470338232689924	3.219730508556434E-4
44-45	0.0	0.0	0.0	0.3214900912793599	3.219730508556434E-4
46-47	0.0	0.0	0.0	0.40045398200170645	4.024663135695542E-4
48-49	0.0	0.0	0.0	0.4904454497158588	4.8295957628346505E-4
50-51	0.0	0.0	0.0	0.5891301898031135	4.8295957628346505E-4
52-53	0.0	0.0	0.0	0.6937714313311976	8.049326271391085E-4
54-55	0.0	0.0	0.0	0.803564241672972	8.049326271391085E-4
56-57	0.0	0.0	0.0	0.938068483667917	8.854258898530193E-4
58-59	0.0	0.0	0.0	1.0909251895616336	9.659191525669301E-4
60-61	0.0	0.0	0.0	1.2598805479981325	9.659191525669301E-4
62-63	0.0	0.0	0.0	1.4304457716889096	9.659191525669301E-4
64-65	0.0	0.0	0.0	1.6064845372442327	9.659191525669301E-4
66-67	0.0	0.0	0.0	1.8059468422493037	9.659191525669301E-4
68-69	0.0	0.0	0.0	2.0335012959415297	9.659191525669301E-4
70-71	0.0	0.0	0.0	2.293092068193892	9.659191525669301E-4
72-73	0.0	0.0	0.0	2.55855884862437	9.659191525669301E-4
74-75	0.0	0.0	0.0	2.8216913244361446	0.001046412415280841
76-77	0.0	0.0	0.0	3.110340164528229	0.0011269056779947518
78-79	0.0	0.0	0.0	3.4380282370365602	0.0011269056779947518
80-81	0.0	0.0	0.0	3.806123927427274	0.0011269056779947518
82-83	0.0	0.0	0.0	4.2007019012508655	0.0014488787288503952
84-85	0.0	0.0	0.0	4.58505723070979	0.0014488787288503952
86-87	0.0	0.0	0.0	4.993721525508315	0.0014488787288503952
88-89	0.0	0.0	0.0	5.452372136452179	0.0014488787288503952
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	480	0.0	44.589638	1
CCGTGCG	65	2.5885001E-6	43.84355	9
TAGCGTG	70	2.0549071E-4	33.9211	5
ACCGTAT	80	4.4970884E-4	29.68096	8
GGACCGC	65	0.005848326	29.224329	6
AGGACCG	85	6.412095E-4	27.93502	5
ATCAACG	765	0.0	27.314241	4
ACCGTGC	140	5.810052E-7	27.136879	8
GTATCAA	775	0.0	26.9618	2
TCAACGC	775	0.0	26.9618	5
AAACCGT	95	0.0012274955	24.994493	6
GTACTAC	115	1.2694809E-4	24.815102	1
CAACGCA	855	0.0	24.43906	6
TCGCACC	260	2.3646862E-11	23.781137	1
GTACATG	1785	0.0	23.181614	1
GTATATA	415	0.0	22.92158	1
ACATGGG	1575	0.0	22.915585	3
CGCACCA	270	4.0017767E-11	22.865332	2
ACGCAGA	900	0.0	22.689445	8
AACGCAG	900	0.0	22.689445	7
>>END_MODULE