FastQCFastQC Report
Fri 10 Feb 2017
SRR3224484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621170
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG500.001584884138.1470341
ATAACGC500.001616602837.9934963
GTATCAA8250.033.5231481
GGTATCA5450.033.247411
TCAACGC8000.032.650664
TAACGCT600.003952876731.6612474
ATCAACG8300.031.4705183
CAACGCA9100.028.181995
AACGCAG9300.028.086596
CGCAGAG9350.027.428468
TAGCGTT1109.4807794E-525.9046576
ACCGTGC1109.4807794E-525.9046578
ACGCAGA10000.025.645617
GTACATG17750.024.4463351
ACATGGG16950.023.8159833
CTTACAC5000.023.7459353
TATCAAC11250.023.6403962
ATAGCGT1252.256897E-422.7960995
GTGTAGC4400.022.7581711
GAGTACA11950.022.3455411
Produced by FastQC (version 0.11.5)