##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621170
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19494180337106	34.0	33.0	34.0	31.0	34.0
2	33.33302316596101	34.0	34.0	34.0	31.0	34.0
3	33.45205821272759	34.0	34.0	34.0	31.0	34.0
4	36.6450971553681	37.0	37.0	37.0	35.0	37.0
5	36.686288777629315	37.0	37.0	37.0	35.0	37.0
6	36.72834650739733	37.0	37.0	37.0	37.0	37.0
7	36.709082859764635	37.0	37.0	37.0	36.0	37.0
8	36.69149991145741	37.0	37.0	37.0	36.0	37.0
9	38.54644783231644	39.0	39.0	39.0	38.0	39.0
10-11	38.584259542476296	39.0	39.0	39.0	37.5	39.0
12-13	38.59515913518038	39.0	39.0	39.0	38.0	39.0
14-15	40.24872418178599	41.0	40.0	41.0	39.0	41.0
16-17	40.25829966031843	41.0	40.0	41.0	39.0	41.0
18-19	40.25974853904728	41.0	40.0	41.0	39.0	41.0
20-21	40.26013893137144	41.0	40.0	41.0	39.0	41.0
22-23	40.20249126648099	41.0	40.0	41.0	39.0	41.0
24-25	40.19533300062785	41.0	40.0	41.0	39.0	41.0
26-27	40.156008822061594	41.0	40.0	41.0	39.0	41.0
28-29	40.128715971473184	41.0	40.0	41.0	38.5	41.0
30-31	40.0840373810712	41.0	40.0	41.0	38.0	41.0
32-33	40.06021539997102	41.0	40.0	41.0	38.0	41.0
34-35	40.00347167442085	41.0	40.0	41.0	38.0	41.0
36-37	39.93711705330264	41.0	40.0	41.0	38.0	41.0
38-39	39.883659867669074	41.0	40.0	41.0	38.0	41.0
40-41	39.80175958272292	41.0	40.0	41.0	38.0	41.0
42-43	39.75248402208735	41.0	40.0	41.0	38.0	41.0
44-45	39.60424923933867	41.0	40.0	41.0	37.0	41.0
46-47	39.46500957869826	41.0	40.0	41.0	37.0	41.0
48-49	39.44219698311251	41.0	40.0	41.0	36.0	41.0
50-51	39.38430139897291	41.0	39.0	41.0	36.0	41.0
52-53	39.26170854999437	41.0	39.0	41.0	35.5	41.0
54-55	39.16029267350322	41.0	39.0	41.0	35.0	41.0
56-57	39.03439879582079	41.0	39.0	41.0	35.0	41.0
58-59	38.86882978894666	40.0	38.0	41.0	35.0	41.0
60-61	38.70924465122269	40.0	38.0	41.0	35.0	41.0
62-63	38.54340116232271	40.0	37.0	41.0	35.0	41.0
64-65	38.340978315115024	40.0	37.0	41.0	35.0	41.0
66-67	38.10034612102967	40.0	36.0	41.0	34.5	41.0
68-69	37.864977381393174	39.0	36.0	41.0	34.0	41.0
70-71	37.5703076452501	39.0	35.5	41.0	34.0	41.0
72-73	37.26219231450328	38.5	35.0	40.5	34.0	41.0
74-75	36.9128869713605	37.5	35.0	40.0	34.0	41.0
76-77	34.89433327430494	35.5	33.0	38.0	30.5	39.0
78-79	36.11610750680168	36.0	35.0	39.0	33.5	40.0
80-81	35.91886279118438	36.0	35.0	38.5	34.0	39.0
82-83	35.58010850491814	36.0	35.0	37.0	34.0	39.0
84-85	35.250717194970775	35.0	35.0	37.0	33.5	39.0
86-87	34.94860827148768	35.0	35.0	36.5	33.0	37.0
88-89	34.69595199381812	35.0	35.0	36.0	33.0	37.0
90-91	34.49534426968463	35.0	35.0	36.0	33.0	37.0
92-93	34.34801906080461	35.0	35.0	35.5	33.0	36.0
94-95	34.20871259075615	35.0	35.0	35.0	33.0	36.0
96-97	34.121037719142905	35.0	35.0	35.0	33.0	36.0
98-99	34.047616594491046	35.0	35.0	35.0	33.0	36.0
100-101	33.30647568298534	34.5	34.0	35.0	30.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	7.0
15	26.0
16	59.0
17	119.0
18	185.0
19	309.0
20	423.0
21	582.0
22	775.0
23	794.0
24	840.0
25	1023.0
26	1296.0
27	1623.0
28	2205.0
29	2648.0
30	3034.0
31	3776.0
32	4692.0
33	6523.0
34	10414.0
35	19147.0
36	45001.0
37	109838.0
38	261766.0
39	144039.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63280623204535	21.2526527601646	14.267805843836538	22.846735163953518
2	14.179693159682536	20.993286861889658	42.82241576380057	22.004604214627236
3	18.06542492393387	27.18273580501312	30.799942044850848	23.951897226202167
4	11.454352270714942	17.87385095867476	41.48815944105478	29.183637329555516
5	12.072057568781492	39.07159070785775	36.96975063187211	11.886601091488643
6	28.15396751291917	39.648727401516496	19.819373118469986	12.377931967094353
7	25.211133828098585	33.22729687525155	24.41505545985801	17.14651383679186
8	23.28267623999871	38.20290741664923	21.58958095207431	16.92483539127775
9	24.13574383824074	17.819276526554727	21.955825297422606	36.08915433778193
10-11	23.208944411352768	27.46301334578296	30.59299386641338	18.73504837645089
12-13	23.631212067549946	25.0364634480094	30.488674597936154	20.8436498865045
14-15	20.893153243073552	27.38767165188274	27.28150103836309	24.437674066680618
16-17	20.7759550525621	30.591384001159106	28.101968865205983	20.530692081072814
18-19	20.807347425020524	29.67255340727981	29.148783746800394	20.371315420899272
20-21	21.417112536212226	28.680244927068433	29.38118646509902	20.52145607162032
22-23	21.10976954593385	28.708716685582736	29.19538286928594	20.986130899197477
24-25	21.061519786981258	28.779907464349304	28.94161947047971	21.216953278189727
26-27	20.959150998907678	29.198096766741234	28.96103781110466	20.88171442324643
28-29	20.95979354223819	28.860537975091123	29.232593408291795	20.947075074378887
30-31	21.27544313945823	28.434335832134643	29.083519743964764	21.206701284442367
32-33	20.87137316897656	28.89173477749212	29.193182531645977	21.043709521885347
34-35	21.414179792302757	28.843449471097305	28.6207154695888	21.12165526701114
36-37	21.105194737244577	28.46862510715881	29.004801519743385	21.421378635853223
38-39	21.040133940789154	28.778112272002833	28.911248128531643	21.27050565867637
40-41	21.292641305922693	28.594668126277828	29.126165139977783	20.986525427821693
42-43	21.141394465283255	28.469581596020415	29.12729204565578	21.261731893040555
44-45	21.319734024568916	28.441982901579433	29.042359646439444	21.195923427412215
46-47	21.56673519233372	28.35593176647999	28.717705399779604	21.35962764140669
48-49	21.027695472952903	28.379267072515983	29.140818122033302	21.45221933249781
50-51	21.142762850749392	28.49195872305488	28.99761739942367	21.36766102677206
52-53	21.213113962361348	28.637973501617914	28.779722137257114	21.369190398763624
54-55	21.10291868570601	28.48535827551234	28.958739153532846	21.452983885248806
56-57	21.25194406251912	28.687681325085894	28.59277243440097	21.467602177994017
58-59	21.285802861813234	28.481644616586276	28.838525771618606	21.394026749981883
60-61	21.372135546875942	28.56864502238744	28.62379840111563	21.435421029620986
62-63	20.989342692016677	28.56907126873481	28.930807991371122	21.510778047877395
64-65	21.284833459439444	28.48632419466491	28.690213629119242	21.538628716776405
66-67	21.006165783923887	28.746558913018983	28.549833378946182	21.69744192411095
68-69	21.372329636009464	28.338377577796734	28.79002527488449	21.499267511309302
70-71	21.394787256306646	28.24854709660801	28.789944781621777	21.56672086546356
72-73	21.05422026176409	28.42635671394304	28.832928183911005	21.686494840381858
74-75	21.164818004733004	28.29370381699052	28.701160712848335	21.840317465428143
76-77	21.00368739933303	28.35981161341631	28.75970246446421	21.87679852278645
78-79	21.365741430205574	28.073296566447254	28.407370228482236	22.153591774864942
80-81	20.97906882767081	28.472093648521806	28.45454501546373	22.09429250834365
82-83	20.93486004064959	28.535689131265972	28.571911536836176	21.957539291248267
84-85	21.11976431098393	28.33959181045065	28.567150843385132	21.97349303518029
86-87	20.624386238871807	28.593380234074406	28.764186937553326	22.018046589500457
88-89	21.21681665244619	27.932611040455914	28.3890883333065	22.461483973791392
90-91	21.060659722781203	28.155738364698873	28.31229776067743	22.471304151842492
92-93	21.090764203036205	28.059790395543892	28.66155802759309	22.18788737382681
94-95	21.280969782829175	27.826842893249836	28.28879372796497	22.603393595956017
96-97	21.187517104818326	27.925608126599805	28.554260508395444	22.332614260186425
98-99	20.742308868747685	28.086997118341195	28.57679862195534	22.593895390955776
100-101	20.94379242269374	28.07745003515843	28.385458827585154	22.59329871456268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	1.0
5	2.0
6	4.5
7	6.0
8	11.0
9	14.0
10	12.0
11	11.0
12	9.5
13	12.5
14	18.5
15	20.5
16	20.5
17	23.0
18	37.0
19	52.0
20	51.0
21	57.0
22	75.0
23	96.0
24	115.5
25	145.0
26	203.5
27	294.0
28	506.0
29	1035.5
30	2243.5
31	4673.0
32	8960.0
33	15033.0
34	22505.0
35	30631.0
36	37964.0
37	42592.5
38	44701.5
39	44757.0
40	40398.5
41	33454.5
42	28076.5
43	23960.0
44	20357.0
45	17665.5
46	15505.5
47	13773.5
48	12717.0
49	12770.5
50	13419.5
51	13277.5
52	12684.5
53	12742.0
54	13166.0
55	12859.5
56	11349.0
57	9980.5
58	9366.5
59	8644.0
60	7084.5
61	5451.5
62	4472.0
63	3234.0
64	2102.5
65	1452.0
66	1069.0
67	913.5
68	721.0
69	489.0
70	340.5
71	222.5
72	147.5
73	131.0
74	93.0
75	66.5
76	49.0
77	25.0
78	12.5
79	7.0
80	9.0
81	4.5
82	1.5
83	1.5
84	2.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4742663039103627
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.854258898530193E-4
22-23	8.049326271391085E-4
24-25	6.439461017112867E-4
26-27	0.002656277669559058
28-29	0.004185649661123364
30-31	8.854258898530193E-4
32-33	1.6098652542782167E-4
34-35	0.004105156398409453
36-37	0.0020123315678477714
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0096591915256693
46-47	0.07695155915449878
48-49	5.634528389973759E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007727353220535442
58-59	0.0378318334755381
60-61	0.028092148687154882
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	8.049326271391084E-5
78-79	0.007968833008677173
80-81	0.006278474491685045
82-83	0.0012073989407086626
84-85	0.0020123315678477714
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06576299563726516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	621170.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.14345873741144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11930211408021	38.326691560705314
2	14.007322982317252	14.887951818647437
3	5.392097546089385	8.59664140346096
4	2.8140147787691303	5.9818591312793306
5	1.603701094543164	4.26131114724981
6	1.0125359723132963	3.228579819886586
7	0.691081047254474	2.5708505983282657
8	0.48689601125595183	2.0700270466872714
9	0.33710927310916916	1.6123637470928303
>10	1.42356851639525	12.662396632225533
>50	0.07926688925877598	2.8848471971839786
>100	0.03310377461391772	2.91647989725263
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.609865254278217E-4
2	0.0	0.0	0.0	0.0	1.609865254278217E-4
3	0.0	0.0	0.0	0.0	1.609865254278217E-4
4	0.0	0.0	0.0	0.0	1.609865254278217E-4
5	0.0	0.0	0.0	0.0	1.609865254278217E-4
6	0.0	0.0	0.0	0.0	1.609865254278217E-4
7	0.0	0.0	0.0	0.0	1.609865254278217E-4
8	0.0	0.0	0.0	0.0	1.609865254278217E-4
9	0.0	0.0	0.0	0.0	1.609865254278217E-4
10-11	0.0	0.0	0.0	0.0	1.609865254278217E-4
12-13	0.0	0.0	0.0	0.0	3.219730508556434E-4
14-15	0.0	0.0	0.0	0.0	3.219730508556434E-4
16-17	0.0	0.0	0.0	0.0	3.219730508556434E-4
18-19	0.0	0.0	0.0	1.609865254278217E-4	3.219730508556434E-4
20-21	0.0	0.0	0.0	1.609865254278217E-4	4.8295957628346505E-4
22-23	0.0	0.0	0.0	4.8295957628346505E-4	4.8295957628346505E-4
24-25	0.0	0.0	0.0	8.049326271391084E-4	4.8295957628346505E-4
26-27	0.0	0.0	0.0	0.0016903585169921278	4.8295957628346505E-4
28-29	0.0	0.0	0.0	0.0035417035594120776	4.8295957628346505E-4
30-31	0.0	0.0	0.0	0.009256725212099748	4.8295957628346505E-4
32-33	0.0	0.0	0.0	0.02382600576331761	4.8295957628346505E-4
34-35	0.0	0.0	0.0	0.04322488207737013	4.8295957628346505E-4
36-37	0.0	0.0	0.0	0.07558317368836229	4.8295957628346505E-4
38-39	0.0	0.0	0.0	0.12677688877440957	4.8295957628346505E-4
40-41	0.0	0.0	0.0	0.18722732907255663	4.8295957628346505E-4
42-43	0.0	0.0	0.0	0.2488046750486984	4.8295957628346505E-4
44-45	0.0	0.0	0.0	0.32350242284720765	4.8295957628346505E-4
46-47	0.0	0.0	0.0	0.40423716534926024	4.8295957628346505E-4
48-49	0.0	0.0	0.0	0.49495307242783776	4.8295957628346505E-4
50-51	0.0	0.0	0.0	0.595891623871082	4.8295957628346505E-4
52-53	0.0	0.0	0.0	0.701498784551733	4.8295957628346505E-4
54-55	0.0	0.0	0.0	0.8120965275206465	4.8295957628346505E-4
56-57	0.0	0.0	0.0	0.9481301415071559	4.8295957628346505E-4
58-59	0.0	0.0	0.0	1.1017917800280115	4.8295957628346505E-4
60-61	0.0	0.0	0.0	1.27114960477808	4.8295957628346505E-4
62-63	0.0	0.0	0.0	1.4432442004604216	4.8295957628346505E-4
64-65	0.0	0.0	0.0	1.6200878986428835	4.8295957628346505E-4
66-67	0.0	0.0	0.0	1.8199526699615243	4.8295957628346505E-4
68-69	0.0	0.0	0.0	2.0500024147978815	4.8295957628346505E-4
70-71	0.0	0.0	0.0	2.311525025355378	5.634528389973759E-4
72-73	0.0	0.0	0.0	2.5790041373537034	7.244393644251976E-4
74-75	0.0	0.0	0.0	2.843987958207898	8.049326271391085E-4
76-77	0.0	0.0	0.0	3.13464912986783	8.049326271391085E-4
78-79	0.0	0.0	0.0	3.4627396686897307	8.049326271391085E-4
80-81	0.0	0.0	0.0	3.8322037445465815	8.049326271391085E-4
82-83	0.0	0.0	0.0	4.227345171209169	8.049326271391085E-4
84-85	0.0	0.0	0.0	4.6157251638037895	8.049326271391085E-4
86-87	0.0	0.0	0.0	5.025918830593879	8.854258898530193E-4
88-89	0.0	0.0	0.0	5.487628185520872	9.659191525669301E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	50	0.0015848841	38.147034	1
ATAACGC	50	0.0016166028	37.993496	3
GTATCAA	825	0.0	33.523148	1
GGTATCA	545	0.0	33.24741	1
TCAACGC	800	0.0	32.65066	4
TAACGCT	60	0.0039528767	31.661247	4
ATCAACG	830	0.0	31.470518	3
CAACGCA	910	0.0	28.18199	5
AACGCAG	930	0.0	28.08659	6
CGCAGAG	935	0.0	27.42846	8
TAGCGTT	110	9.4807794E-5	25.904657	6
ACCGTGC	110	9.4807794E-5	25.904657	8
ACGCAGA	1000	0.0	25.64561	7
GTACATG	1775	0.0	24.446335	1
ACATGGG	1695	0.0	23.815983	3
CTTACAC	500	0.0	23.745935	3
TATCAAC	1125	0.0	23.640396	2
ATAGCGT	125	2.256897E-4	22.796099	5
GTGTAGC	440	0.0	22.758171	1
GAGTACA	1195	0.0	22.345541	1
>>END_MODULE