FastQCFastQC Report
Fri 10 Feb 2017
SRR3224483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024899
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG14210.13864780822305417No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11790.11503572547148548No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11470.111913466595245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.048.023231
GTATCAA20200.043.7815931
ATCAACG22700.038.2790873
TCAACGC23350.037.4168554
TATCAAC25800.033.8637052
CCGTGCG1201.5019577E-731.6752919
CAACGCA27750.031.3129845
AATGCGG2350.030.3081727
CGTTATA953.4991157E-529.9891412
AACGCAG29100.029.8603216
ATGCGGG2250.029.5477378
CGCAGAG29600.029.3587868
AGCGTAC650.005851339529.2230388
ACGCAGA30150.028.8204067
CGTACGA350.00838093627.15091310-11
GTACATG50050.025.8401371
GCAGAGT34750.024.8844749
TACATGG50600.024.4921192
ACATGGG49400.024.3181153
AAATGCG3050.023.3521966
Produced by FastQC (version 0.11.5)