##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224483_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1024899
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40388077264199	34.0	31.0	34.0	31.0	34.0
2	32.56375701410578	34.0	31.0	34.0	31.0	34.0
3	32.712446787439546	34.0	33.0	34.0	31.0	34.0
4	35.980382457198225	37.0	37.0	37.0	35.0	37.0
5	36.00602205680755	37.0	37.0	37.0	35.0	37.0
6	36.11687590679667	37.0	37.0	37.0	35.0	37.0
7	36.08006349893989	37.0	37.0	37.0	35.0	37.0
8	36.117205695390474	37.0	37.0	37.0	35.0	37.0
9	37.87000670309953	39.0	39.0	39.0	37.0	39.0
10-11	37.95510777159505	39.0	39.0	39.0	37.0	39.0
12-13	37.975272197553124	39.0	39.0	39.0	37.0	39.0
14-15	39.51135380169168	41.0	40.0	41.0	38.0	41.0
16-17	39.35392267921034	41.0	40.0	41.0	37.5	41.0
18-19	39.460250717387765	41.0	40.0	41.0	37.0	41.0
20-21	39.538680396800075	41.0	40.0	41.0	38.0	41.0
22-23	39.50160113337998	41.0	40.0	41.0	37.5	41.0
24-25	39.46339980817622	41.0	40.0	41.0	37.0	41.0
26-27	39.33159852824522	41.0	40.0	41.0	37.0	41.0
28-29	39.270921329809084	41.0	40.0	41.0	36.5	41.0
30-31	39.208934734056726	41.0	40.0	41.0	36.0	41.0
32-33	39.03747198504438	41.0	39.5	41.0	36.0	41.0
34-35	39.05629335183272	41.0	39.0	41.0	36.0	41.0
36-37	39.07942050875257	41.0	39.0	41.0	36.0	41.0
38-39	38.99956873799272	41.0	39.0	41.0	35.5	41.0
40-41	38.94104931315184	41.0	39.0	41.0	35.0	41.0
42-43	38.836644879153944	41.0	39.0	41.0	35.0	41.0
44-45	38.71579687364316	41.0	39.0	41.0	35.0	41.0
46-47	38.605356722955136	40.0	39.0	41.0	35.0	41.0
48-49	38.45590150834375	40.0	38.0	41.0	34.5	41.0
50-51	37.24157063281358	39.0	36.5	40.0	32.5	40.5
52-53	37.586932956320574	39.5	37.5	40.5	33.5	40.5
54-55	38.11034062868634	40.0	38.0	41.0	34.0	41.0
56-57	38.04222220921281	40.0	37.0	41.0	34.0	41.0
58-59	37.963604706414976	40.0	37.0	41.0	34.0	41.0
60-61	37.79011444054487	40.0	36.5	41.0	33.5	41.0
62-63	37.56895996581126	40.0	36.0	41.0	33.0	41.0
64-65	37.38677567252968	40.0	35.0	41.0	33.0	41.0
66-67	37.18979772641012	39.0	35.0	41.0	33.0	41.0
68-69	36.92308900681921	39.0	35.0	41.0	32.5	41.0
70-71	36.64703595183526	38.5	35.0	41.0	32.0	41.0
72-73	36.324613937568486	37.5	35.0	40.0	32.0	41.0
74-75	35.98925601449508	37.0	35.0	39.5	32.0	41.0
76-77	35.5811801943411	36.5	35.0	39.0	31.0	41.0
78-79	35.24591935400464	36.0	35.0	39.0	31.0	39.5
80-81	34.84586822701554	36.0	35.0	37.0	31.0	39.0
82-83	34.49141622735509	35.0	35.0	37.0	31.0	39.0
84-85	34.17486893830514	35.0	34.5	37.0	30.5	38.0
86-87	33.87608583870215	35.0	34.0	36.0	30.0	37.0
88-89	33.66075974315518	35.0	34.0	36.0	30.0	37.0
90-91	33.45130056717784	35.0	34.0	35.5	30.0	36.5
92-93	33.27184678685412	35.0	34.0	35.0	29.0	36.0
94-95	33.122096909061284	35.0	34.0	35.0	29.0	36.0
96-97	32.98383499252121	35.0	34.0	35.0	29.0	36.0
98-99	32.918616370978995	35.0	34.0	35.0	29.0	35.5
100-101	31.827634235178294	34.5	32.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	136.0
8	610.0
9	473.0
10	411.0
11	760.0
12	1097.0
13	1249.0
14	1351.0
15	1414.0
16	1572.0
17	1640.0
18	1830.0
19	2185.0
20	2538.0
21	3294.0
22	3789.0
23	3882.0
24	3866.0
25	4166.0
26	4460.0
27	4815.0
28	5161.0
29	5964.0
30	7321.0
31	9273.0
32	12428.0
33	17331.0
34	27364.0
35	48322.0
36	100399.0
37	194353.0
38	389229.0
39	162189.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02266910706311	21.04265384318466	14.418404211345107	22.516272838407122
2	14.453814473426164	21.030072231507692	41.52594548340861	22.99016781165754
3	19.270795727965123	27.523519413639214	29.94971206890832	23.255972789487345
4	12.318969966796727	17.615004015029772	41.310704762127784	28.75532125604572
5	12.655100648941994	39.45520485433199	35.57931074183895	12.310383754887066
6	27.115940204839696	39.45559513669152	20.305317889860365	13.123146768608418
7	25.34757083380899	32.97290757430732	23.76058518936988	17.918936402513808
8	24.116815413030942	36.724399184700154	21.947723629352748	17.21106177291616
9	25.57032449051077	16.95064586851973	21.954943853004053	35.52408578796545
10-11	24.270781803865553	27.053592597904768	29.261761402830917	19.413864195398766
12-13	24.601155821207747	24.664283992861733	29.624187358949516	21.110372826981
14-15	21.961163447042996	26.438655200534495	26.761683102746687	24.838498249675826
16-17	21.313737973000155	29.66214065462811	27.665956487012693	21.358164885359038
18-19	21.372088313728934	29.02339108518337	28.812585853114626	20.791934747973073
20-21	22.75004868202269	27.387115052090984	28.442551064879595	21.42028520100673
22-23	22.504204347136284	27.868829251028203	28.215721451323546	21.411244950511964
24-25	21.62677656206696	28.15072623866533	28.464661979912798	21.757835219354916
26-27	21.818345027168533	28.60994107712077	28.38840705279252	21.18330684291818
28-29	21.59368874396404	28.68209452833889	28.425971729897288	21.298244997799785
30-31	21.76780346160939	27.888650491414275	28.661702275053447	21.681843771922892
32-33	21.666720330491103	28.07198562980352	28.615990453693485	21.64530358601189
34-35	22.029536568969235	27.977195801732655	28.35591604636164	21.637351582936464
36-37	21.703309974718827	27.94567646049896	28.756372139277264	21.59464142550495
38-39	21.587219499581643	28.165498601004547	28.232045411855967	22.015236487557843
40-41	22.060625102759992	27.85104399274228	28.666879057647566	21.421451846850164
42-43	21.809116322637614	28.01412869012016	28.57420245593322	21.602552531309
44-45	21.820269167866915	27.99720527562774	28.21242052014661	21.97010503635874
46-47	22.04373308979714	27.74483144192745	28.05295936477643	22.158476103498977
48-49	21.467529971245945	27.76146722750242	28.47563516014749	22.295367641104146
50-51	21.39820606713442	28.15116416349318	28.350891160982695	22.099738608389703
52-53	21.904255931560083	27.999441896225875	28.063204276714092	22.03309789549995
54-55	21.75428993491066	27.892699670894398	28.163750769588024	22.189259624606912
56-57	21.649255194902132	27.95041267480991	28.170727066764627	22.229605063523334
58-59	21.77773614126099	28.149050272678522	28.029605515146987	22.043608070913496
60-61	21.60608673291888	28.125669389848255	28.32606549786015	21.942178379372717
62-63	21.527826238447314	28.060134716052467	28.277292737308578	22.134746308191637
64-65	21.87853549921859	27.696399269448115	28.119072929265126	22.305992302068173
66-67	21.466909203045496	27.867498238553523	28.03027345846597	22.635319099935007
68-69	21.604811791210647	27.743270312489326	28.73668527337816	21.915232622921867
70-71	21.84166439815045	27.76863866585878	28.396261485278064	21.993435450712703
72-73	21.792391250259783	27.759271889230057	28.412360632608674	22.03597622790148
74-75	21.743996237678054	27.708974250145623	28.426264441666937	22.12076507050939
76-77	21.779316791215525	27.64257746373057	27.914555483028085	22.66355026202582
78-79	21.704586651911466	27.203289472721178	28.365603013886187	22.72652086148117
80-81	21.776716473863303	27.672843305755595	28.13095366520016	22.419486555180942
82-83	21.382540213250422	27.89719660380766	28.394471791542706	22.325791391399207
84-85	21.61765043170105	27.444748462707015	28.65325765572152	22.284343449870413
86-87	21.358736586630243	27.58696266588695	28.534776523973093	22.519524223509706
88-89	21.713744989880936	27.52931404619485	28.121768403052457	22.63517256087176
90-91	21.634668391714694	27.226048615522114	28.346061416783506	22.793221575979683
92-93	21.770453509849514	27.101797003140877	28.66613717016645	22.46161231684316
94-95	21.79966025920603	27.091791483843775	28.407238176639844	22.701310080310353
96-97	21.657207197977556	27.30488565214719	28.3751374525685	22.662769697306757
98-99	21.328463221942627	27.308416212065477	28.394580496852832	22.96854006913906
100-101	21.72143909274333	27.067522019110722	28.260746487630932	22.950292400515007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	2.0
6	5.5
7	5.0
8	7.0
9	16.0
10	21.5
11	25.0
12	27.5
13	40.5
14	44.5
15	46.5
16	55.5
17	53.5
18	55.0
19	59.5
20	78.5
21	101.0
22	121.5
23	139.0
24	166.0
25	224.0
26	297.0
27	373.0
28	473.0
29	646.0
30	1015.0
31	2035.5
32	4608.0
33	9776.0
34	18735.0
35	31571.0
36	48373.0
37	62922.5
38	71976.5
39	75486.0
40	72570.0
41	67110.0
42	59610.0
43	50832.0
44	42833.0
45	36785.0
46	32790.5
47	28411.5
48	25088.0
49	23992.5
50	23756.0
51	22721.0
52	21543.5
53	22060.5
54	23206.5
55	23453.5
56	19925.5
57	16602.5
58	15767.0
59	15076.5
60	12894.0
61	9838.5
62	7903.0
63	5751.0
64	3571.0
65	2421.0
66	2033.5
67	1780.5
68	1417.5
69	977.0
70	604.5
71	425.0
72	341.5
73	306.5
74	240.0
75	157.5
76	118.5
77	87.5
78	67.0
79	51.5
80	44.5
81	32.5
82	20.0
83	15.5
84	11.5
85	9.0
86	7.5
87	6.0
88	3.0
89	3.0
90	4.5
91	4.5
92	5.5
93	4.5
94	5.0
95	6.5
96	3.0
97	3.5
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1529906849357839
2	0.0
3	9.757058988251525E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.036540185911001964
16-17	0.07269008946247386
18-19	0.025026856304865164
20-21	0.03839402711876975
22-23	0.06454294520728383
24-25	0.016294288510380044
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02180702683874216
38-39	0.0055127383283621115
40-41	0.005805450098009658
42-43	0.0033174000560055187
44-45	0.010830335476959193
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015367367906496152
60-61	0.017416350294028972
62-63	0.02922239166981332
64-65	0.045663036065017135
66-67	0.016879712049675137
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017123638524381427
80-81	0.056834868606565135
82-83	0.050395209674319125
84-85	0.028246685770988166
86-87	0.058005715685155314
88-89	0.03390577998417405
90-91	0.0
92-93	0.002536835336945397
94-95	0.0
96-97	0.0
98-99	7.80564719060122E-4
100-101	0.043711624267366835
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1024899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.027575667934094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.484746105737436	22.8720149408404
2	15.007019581565965	10.81333067050073
3	6.695009870852228	7.236149241590831
4	3.8231262584723495	5.509518822607133
5	2.435814083214469	4.38782380980144
6	1.7600004716434956	3.804513010064144
7	1.2124447315848297	3.057701101725235
8	0.8902450428709612	2.5658696516029433
9	0.707549169592373	2.294215312164584
>10	3.657734381147553	23.37791365268054
>50	0.20665645406072408	5.114307656365484
>100	0.11443957601874506	7.537144486387162
>500	0.004390966937994368	1.0590226353121475
>1k	8.23306300873944E-4	0.3704750083572605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1421	0.13864780822305417	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1179	0.11503572547148548	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1147	0.111913466595245	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	4.878529494125762E-5	0.0
16-17	0.0	0.0	0.0	2.9271176964754575E-4	0.0
18-19	0.0	0.0	0.0	3.4149706458880334E-4	0.0
20-21	0.0	0.0	0.0	6.342088342363491E-4	0.0
22-23	0.0	0.0	0.0	0.0012684176684726982	0.0
24-25	0.0	0.0	0.0	0.0019514117976503049	0.0
26-27	0.0	0.0	0.0	0.0032198294661230035	0.0
28-29	0.0	0.0	0.0	0.0055127383283621115	0.0
30-31	0.0	0.0	0.0	0.013708667878493393	0.0
32-33	0.0	0.0	0.0	0.03171044171181746	0.0
34-35	0.0	0.0	0.0	0.05571280682291621	0.0
36-37	0.0	0.0	0.0	0.09581431926462998	0.0
38-39	0.0	0.0	0.0	0.16362587923297808	0.0
40-41	0.0	0.0	0.0	0.24343862175687553	0.0
42-43	0.0	0.0	0.0	0.3248124937188933	0.0
44-45	0.0	0.0	0.0	0.41833390412128413	0.0
46-47	0.0	0.0	0.0	0.5270763265453473	0.0
48-49	0.0	0.0	0.0	0.6395752166798875	0.0
50-51	0.0	0.0	0.0	0.7673926894259825	0.0
52-53	0.0	0.0	0.0	0.8960395121860788	0.0
54-55	0.0	0.0	0.0	1.0353703145383106	0.0
56-57	0.0	0.0	0.0	1.1996791879004662	0.0
58-59	0.0	0.0	0.0	1.3784772938601755	0.0
60-61	0.0	0.0	0.0	1.571715847122497	0.0
62-63	0.0	0.0	0.0	1.7691011504548253	0.0
64-65	0.0	0.0	0.0	1.9754141627614037	0.0
66-67	0.0	0.0	0.0	2.205583184294257	0.0
68-69	0.0	0.0	0.0	2.467657788718693	0.0
70-71	0.0	0.0	0.0	2.758076649504	0.0
72-73	0.0	0.0	0.0	3.065033725274393	0.0
74-75	0.0	0.0	0.0	3.3806257982493886	0.0
76-77	0.0	0.0	0.0	3.7120243067853513	0.0
78-79	0.0	0.0	0.0	4.096891498576933	0.0
80-81	0.0	0.0	0.0	4.528446217627298	0.0
82-83	0.0	0.0	0.0	4.978246636985693	0.0
84-85	0.0	0.0	0.0	5.431559597579859	0.0
86-87	0.0	0.0	0.0	5.910240911543479	0.0
88-89	0.0	0.0	0.0	6.456148361936151	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	48.02323	1
GTATCAA	2020	0.0	43.781593	1
ATCAACG	2270	0.0	38.279087	3
TCAACGC	2335	0.0	37.416855	4
TATCAAC	2580	0.0	33.863705	2
CCGTGCG	120	1.5019577E-7	31.675291	9
CAACGCA	2775	0.0	31.312984	5
AATGCGG	235	0.0	30.308172	7
CGTTATA	95	3.4991157E-5	29.989141	2
AACGCAG	2910	0.0	29.860321	6
ATGCGGG	225	0.0	29.547737	8
CGCAGAG	2960	0.0	29.358786	8
AGCGTAC	65	0.0058513395	29.223038	8
ACGCAGA	3015	0.0	28.820406	7
CGTACGA	35	0.008380936	27.150913	10-11
GTACATG	5005	0.0	25.840137	1
GCAGAGT	3475	0.0	24.884474	9
TACATGG	5060	0.0	24.492119	2
ACATGGG	4940	0.0	24.318115	3
AAATGCG	305	0.0	23.352196	6
>>END_MODULE