FastQCFastQC Report
Fri 10 Feb 2017
SRR3224483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024899
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG10910.10644951356182414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.047.2612421
GATACCG801.0793914E-535.6158525
TAGTACG801.0793914E-535.6158524
GTATCAA19350.034.4994851
CCGTTAT753.082858E-431.6585359
TCAACGC21700.030.1996174
ATCAACG21900.029.923823
AGTGCGA404.5016196E-429.67987810-11
AGTACGT1056.917198E-527.1358875
CAACGCA24650.026.7781125
GTACATG49450.026.5174621
GTACGTT908.960309E-426.3821136
TACATGG49450.026.024662
CCGTGCG1301.003967E-525.5703549
ACATGGG47850.025.4062213
CATGGGA25150.025.1128374
ATACCGT1901.19398464E-824.993586
TATCAAC26700.024.7221152
AACGCAG26800.024.629876
CGCAGAG27450.024.0466468
Produced by FastQC (version 0.11.5)