##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1024899
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18458209052795	34.0	33.0	34.0	31.0	34.0
2	33.31606919315952	34.0	34.0	34.0	31.0	34.0
3	33.4406600064982	34.0	34.0	34.0	31.0	34.0
4	36.63081240200254	37.0	37.0	37.0	35.0	37.0
5	36.67333561648514	37.0	37.0	37.0	35.0	37.0
6	36.722278975782004	37.0	37.0	37.0	36.0	37.0
7	36.70477871478067	37.0	37.0	37.0	36.0	37.0
8	36.691706207148215	37.0	37.0	37.0	36.0	37.0
9	38.548579908849554	39.0	39.0	39.0	38.0	39.0
10-11	38.58412097192016	39.0	39.0	39.0	37.5	39.0
12-13	38.586401684458664	39.0	39.0	39.0	38.0	39.0
14-15	40.212743402032785	41.0	40.0	41.0	39.0	41.0
16-17	40.229876309763206	41.0	40.0	41.0	39.0	41.0
18-19	40.237893685133855	41.0	40.0	41.0	39.0	41.0
20-21	40.25108278962122	41.0	40.0	41.0	39.0	41.0
22-23	40.184217176521784	41.0	40.0	41.0	39.0	41.0
24-25	40.16291654104453	41.0	40.0	41.0	38.0	41.0
26-27	40.11982888069946	41.0	40.0	41.0	38.0	41.0
28-29	40.09570845517461	41.0	40.0	41.0	38.0	41.0
30-31	40.04649580104966	41.0	40.0	41.0	38.0	41.0
32-33	40.02118306291644	41.0	40.0	41.0	38.0	41.0
34-35	39.955836623901476	41.0	40.0	41.0	38.0	41.0
36-37	39.88029064327314	41.0	40.0	41.0	38.0	41.0
38-39	39.81203221000313	41.0	40.0	41.0	38.0	41.0
40-41	39.71205260225642	41.0	40.0	41.0	37.0	41.0
42-43	39.65227549251195	41.0	40.0	41.0	37.0	41.0
44-45	39.48156745201234	41.0	40.0	41.0	36.5	41.0
46-47	39.32196928672972	41.0	39.5	41.0	36.0	41.0
48-49	39.27228683021449	41.0	39.0	41.0	35.0	41.0
50-51	39.17759262132171	41.0	39.0	41.0	35.0	41.0
52-53	39.01917993870616	41.0	39.0	41.0	35.0	41.0
54-55	38.885679466952354	40.5	38.5	41.0	35.0	41.0
56-57	38.74774733900609	40.0	38.0	41.0	35.0	41.0
58-59	38.56505372724532	40.0	37.0	41.0	35.0	41.0
60-61	38.39906078550179	40.0	37.0	41.0	35.0	41.0
62-63	38.243497164110806	40.0	36.5	41.0	35.0	41.0
64-65	38.04389310556455	40.0	36.0	41.0	34.5	41.0
66-67	37.82191903787593	39.5	35.0	41.0	34.0	41.0
68-69	37.59481275715949	39.0	35.0	41.0	34.0	41.0
70-71	37.321139448862766	39.0	35.0	41.0	34.0	41.0
72-73	37.010897659183975	38.0	35.0	40.0	34.0	41.0
74-75	36.651008050549365	37.0	35.0	39.5	34.0	41.0
76-77	34.66536458714469	35.5	33.0	38.0	30.5	39.0
78-79	35.88458716419862	36.0	35.0	39.0	33.0	39.5
80-81	35.68573098422381	36.0	35.0	38.0	33.0	39.0
82-83	35.35954030592283	35.5	35.0	37.0	33.0	39.0
84-85	35.04718855223783	35.0	35.0	37.0	33.0	38.0
86-87	34.75981438170981	35.0	35.0	36.0	33.0	37.0
88-89	34.5356327794251	35.0	35.0	36.0	33.0	37.0
90-91	34.344111956397654	35.0	35.0	36.0	33.0	36.5
92-93	34.20814050945508	35.0	35.0	35.5	33.0	36.0
94-95	34.0657269643155	35.0	35.0	35.0	33.0	36.0
96-97	33.997201187629216	35.0	35.0	35.0	33.0	36.0
98-99	33.938397344518826	35.0	35.0	35.0	32.5	36.0
100-101	33.18713551286517	34.5	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	0.0
13	3.0
14	16.0
15	39.0
16	106.0
17	223.0
18	431.0
19	613.0
20	791.0
21	1106.0
22	1610.0
23	1659.0
24	1664.0
25	1889.0
26	2509.0
27	3494.0
28	4586.0
29	5449.0
30	5933.0
31	6579.0
32	7829.0
33	10932.0
34	17541.0
35	34386.0
36	88212.0
37	194898.0
38	416561.0
39	215789.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.765226588975466	21.906819578730467	14.84001188110659	21.487941951187473
2	14.04450584886901	21.576077252490244	42.57336576579741	21.806051132843336
3	18.68593880958026	28.62116169495726	30.584964957522644	22.107934537939837
4	11.882146435892707	18.177986318651886	42.463306140409934	27.476561105045473
5	12.073677503832085	39.606048986290354	36.42310120314294	11.897172306734614
6	26.27068618468747	40.05106844674451	20.856006299157283	12.822239069410744
7	24.713947423111936	33.28737758549867	24.61013231547694	17.388542675912454
8	23.65315996990923	37.135366509285305	22.152621868106028	17.058851652699435
9	25.2269735847142	17.344733481055204	22.442991943596393	34.9853009906342
10-11	23.933431489346756	27.60691541312851	29.613698520537145	18.845954576987587
12-13	24.485876169261555	25.040516187448713	30.10969861420491	20.363909029084816
14-15	21.674574762976643	27.02227243855248	27.179653800032977	24.123498998437896
16-17	20.851078984368215	30.139555214708963	28.028225220241215	20.981140580681608
18-19	21.055147873107497	29.234587993548633	29.395969749214313	20.314294384129557
20-21	22.273512279366567	27.737610865556306	29.035945320961275	20.952931534115855
22-23	22.1098741768023	28.200036979560256	28.72848367703672	20.961605166600723
24-25	21.639892418405175	28.251828355351005	28.724754158102794	21.383525068141026
26-27	21.750663129973475	28.967937049853422	28.51607684648218	20.765322973690925
28-29	21.527744914322735	28.692747863495967	28.9048248864361	20.8746823357452
30-31	21.596557687757496	27.947662803659895	29.06978048917325	21.38599901940935
32-33	21.57509486319113	28.27425585205147	29.036234860673805	21.11441442408359
34-35	21.986900681798776	28.200319555062265	28.523484857724817	21.289294905414142
36-37	21.630142065412535	28.113535243150416	28.799176488954803	21.457146202482242
38-39	21.592322755705684	28.20077880844844	28.598574103399454	21.608324332446415
40-41	21.759997814418785	28.176386160977813	28.805277398065567	21.258338626537835
42-43	21.755655923169016	28.066472891475158	28.798935309723202	21.378935875632624
44-45	21.771713531702876	28.11058109132035	28.43347194513685	21.684233431839928
46-47	21.997236705919114	27.777794051594505	28.268742554728842	21.956226687757532
48-49	21.509241949395644	27.66125601526795	28.763274569418044	22.06622746591836
50-51	21.460895171133938	28.031542620297216	28.543105223051246	21.964456985517597
52-53	21.988312994743872	27.996026925579987	28.270834491984086	21.744825587692056
54-55	21.710675881233175	27.965438545651818	28.337328848989024	21.986556724125986
56-57	21.892375036347918	27.759807107213184	28.201836813951353	22.145981042487545
58-59	21.882191025356335	27.970053611648638	28.249946925199914	21.897808437795113
60-61	21.551117927886736	28.25858764889398	28.29450422678586	21.895790196433428
62-63	21.650133330211073	27.977147016437716	28.288836265817412	22.083883387533795
64-65	21.501191824755413	27.927434800892577	28.21102372038611	22.3603496539659
66-67	21.585053746759435	27.939338412858245	28.129649848424087	22.345957991958233
68-69	21.7635591409495	27.769907083527258	28.363282625897774	22.103251149625475
70-71	21.91650104059034	27.904895994629715	28.357818672864347	21.820784291915594
72-73	21.736629658141922	27.872844055853307	28.282494177475048	22.10803210852972
74-75	21.836737083361385	27.661798869937428	28.275664236183275	22.225799810517916
76-77	21.670171090697096	27.492913908254017	28.07629074197845	22.760624259070443
78-79	21.63585481186001	27.45906118330793	28.17786893723983	22.727215067592226
80-81	21.626940856477248	27.87134545720663	27.99038872016295	22.511324966153172
82-83	21.17301886829272	27.969038749373105	28.305658926679243	22.552283455654926
84-85	21.629602815171207	27.464653023708735	28.320708222608502	22.585035938511556
86-87	21.069344393935403	27.82644924036417	28.325278881138534	22.77892748456189
88-89	21.655353356769787	27.419531095259142	27.922556271398452	23.00255927657262
90-91	21.628716585731862	27.437337727912702	27.91367734771914	23.020268338636296
92-93	21.63497165816908	27.265334079423475	28.37124846997044	22.728445792437007
94-95	21.47836030672291	27.260198321980994	27.957486542576394	23.303954828719707
96-97	21.395961943567123	27.485586384609604	28.017736381828843	23.10071528999443
98-99	20.840687716545727	27.67780044667816	28.12608852189338	23.355423314882735
100-101	21.18419922126714	27.415136082981522	28.089009478358335	23.311655217393003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	2.5
3	2.5
4	0.5
5	1.5
6	3.5
7	5.0
8	9.0
9	12.5
10	16.5
11	17.0
12	15.5
13	27.0
14	29.5
15	32.0
16	43.0
17	47.0
18	48.5
19	59.5
20	70.0
21	82.5
22	106.5
23	129.5
24	167.5
25	227.5
26	303.0
27	392.0
28	494.5
29	701.0
30	1222.5
31	2554.0
32	5743.0
33	12158.0
34	22497.5
35	36289.5
36	53453.5
37	67542.0
38	75363.5
39	76889.0
40	71717.0
41	65018.0
42	57267.0
43	48397.0
44	40079.0
45	34054.5
46	29949.0
47	26045.0
48	23262.0
49	22923.5
50	23895.5
51	22675.0
52	20696.5
53	21151.5
54	22749.0
55	22555.0
56	19126.5
57	16262.0
58	15639.0
59	15353.5
60	13146.5
61	9786.5
62	7761.5
63	5575.0
64	3352.5
65	2220.5
66	1782.0
67	1553.5
68	1233.0
69	781.5
70	479.0
71	358.0
72	308.0
73	287.0
74	212.5
75	139.5
76	104.5
77	77.0
78	49.0
79	37.5
80	32.5
81	20.5
82	12.5
83	4.5
84	3.5
85	3.5
86	1.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4675582667170131
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.781353089426373E-4
22-23	8.293500140013797E-4
24-25	2.4392647470628814E-4
26-27	0.0023904794521216236
28-29	0.0034637559408292914
30-31	6.342088342363492E-4
32-33	1.951411797650305E-4
34-35	0.0035613265307118066
36-37	0.0018538412077677897
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009220420743897692
46-47	0.07405607772082907
48-49	6.829941291776068E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007903217780483736
58-59	0.03917459183782988
60-61	0.029515103439460864
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.90282359530061E-4
78-79	0.007512935420953675
80-81	0.006000591277774688
82-83	4.8785294941257627E-4
84-85	0.0022929088622391084
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.878529494125762E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06546986581116773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1024899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.447470086904964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.29818483022485	21.460148584076467
2	15.417742616079464	10.622044551499945
3	6.877211433982562	7.107076054603052
4	3.935567644353393	5.422813948154182
5	2.5185934910078727	4.337958697128362
6	1.7360153616165048	3.5880802343815237
7	1.210278027074483	2.9183711294140635
8	0.92692549390607	2.5544190579295165
9	0.7677586884979132	2.3802610010393925
>10	3.937389640849309	24.153755407559768
>50	0.23073206497020485	5.439704095064422
>100	0.1370734025633633	8.387948272429488
>500	0.006243509009818608	1.5207622662518259
>1k	2.8379586408266397E-4	0.10665670046803431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1091	0.10644951356182414	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	4.878529494125762E-5	0.0
16-17	0.0	0.0	0.0	3.90282359530061E-4	0.0
18-19	0.0	0.0	0.0	4.3906765447131863E-4	0.0
20-21	0.0	0.0	0.0	7.317794241188644E-4	0.0
22-23	0.0	0.0	0.0	0.0013659882583552134	0.0
24-25	0.0	0.0	0.0	0.0020489823875328205	0.0
26-27	0.0	0.0	0.0	0.0032198294661230035	0.0
28-29	0.0	0.0	0.0	0.0055127383283621115	0.0
30-31	0.0	0.0	0.0	0.013708667878493393	0.0
32-33	0.0	0.0	0.0	0.03175922700675872	0.0
34-35	0.0	0.0	0.0	0.05585916270773998	0.0
36-37	0.0	0.0	0.0	0.09630217221404255	0.0
38-39	0.0	0.0	0.0	0.164747941016627	0.0
40-41	0.0	0.0	0.0	0.2457803159140559	0.0
42-43	0.0	0.0	0.0	0.32798353789007506	0.0
44-45	0.0	0.0	0.0	0.42213915712670225	0.0
46-47	0.0	0.0	0.0	0.5320036413344145	0.0
48-49	0.0	0.0	0.0	0.6457221638424859	0.0
50-51	0.0	0.0	0.0	0.7751983366165838	0.0
52-53	0.0	0.0	0.0	0.9042842270311513	0.0
54-55	0.0	0.0	0.0	1.0451761588215034	0.0
56-57	0.0	0.0	0.0	1.2112900880964856	0.0
58-59	0.0	0.0	0.0	1.391649323494315	0.0
60-61	0.0	0.0	0.0	1.586692932669463	0.0
62-63	0.0	0.0	0.0	1.7857857213247355	0.0
64-65	0.0	0.0	0.0	1.9935622924795517	0.0
66-67	0.0	0.0	0.0	2.224999731680878	0.0
68-69	0.0	0.0	0.0	2.4897087420321418	0.0
70-71	0.0	0.0	0.0	2.7822741557948634	0.0
72-73	0.0	0.0	0.0	3.091768066902202	0.0
74-75	0.0	0.0	0.0	3.4077016369417867	0.0
76-77	0.0	0.0	0.0	3.740856416095635	0.0
78-79	0.0	0.0	0.0	4.129431290302752	0.0
80-81	0.0	0.0	0.0	4.561766574072177	0.0
82-83	0.0	0.0	0.0	5.0165430935145805	0.0
84-85	0.0	0.0	0.0	5.474002804178753	0.0
86-87	0.0	0.0	0.0	5.957269935866851	0.0
88-89	0.0	0.0	0.0	6.508397412818239	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1130	0.0	47.261242	1
GATACCG	80	1.0793914E-5	35.615852	5
TAGTACG	80	1.0793914E-5	35.615852	4
GTATCAA	1935	0.0	34.499485	1
CCGTTAT	75	3.082858E-4	31.658535	9
TCAACGC	2170	0.0	30.199617	4
ATCAACG	2190	0.0	29.92382	3
AGTGCGA	40	4.5016196E-4	29.679878	10-11
AGTACGT	105	6.917198E-5	27.135887	5
CAACGCA	2465	0.0	26.778112	5
GTACATG	4945	0.0	26.517462	1
GTACGTT	90	8.960309E-4	26.382113	6
TACATGG	4945	0.0	26.02466	2
CCGTGCG	130	1.003967E-5	25.570354	9
ACATGGG	4785	0.0	25.406221	3
CATGGGA	2515	0.0	25.112837	4
ATACCGT	190	1.19398464E-8	24.99358	6
TATCAAC	2670	0.0	24.722115	2
AACGCAG	2680	0.0	24.62987	6
CGCAGAG	2745	0.0	24.046646	8
>>END_MODULE