FastQCFastQC Report
Fri 10 Feb 2017
SRR3224482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793870
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG20440.2574728859888899No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA15700.1977653772028166No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12020.15141018050814364No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT9990.12583924320102788No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9250.11651781777872951No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA8430.10618867068915565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.045.3860931
GGTATCA5200.040.2420851
AAACCGT1700.036.308966
ATCAACG12400.035.22783
TCAACGC12850.033.624644
CGTCCGA300.003939675631.68589816-17
CCGTGCG1859.094947E-1230.8217479
ACCGTGC2051.8189894E-1230.113668
AACCGTG2051.8189894E-1230.1098677
TCGCACC8250.029.9764751
CGCACCA8650.028.5434592
ACGCAGA16600.026.88687
CTGTGCG9900.026.8782239
CGCAGAG16300.026.8024398
CAACGCA16600.026.3147415
TATCAAC17150.026.30142
GTACATG26600.025.9249271
AACGCAG17250.025.8736766
TACATGG26150.024.5121522
TGTTCCG5500.024.1721215
Produced by FastQC (version 0.11.5)