##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224482_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793870
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.001952460730344	34.0	31.0	34.0	30.0	34.0
2	32.17388993160089	34.0	31.0	34.0	30.0	34.0
3	32.32176804766524	34.0	31.0	34.0	30.0	34.0
4	35.63274465592603	37.0	35.0	37.0	35.0	37.0
5	35.62779548288763	37.0	35.0	37.0	35.0	37.0
6	35.736010933780086	37.0	37.0	37.0	35.0	37.0
7	35.690594177888066	37.0	37.0	37.0	35.0	37.0
8	35.72252383891569	37.0	37.0	37.0	35.0	37.0
9	37.42126544648368	39.0	38.0	39.0	35.0	39.0
10-11	37.49949361986219	39.0	38.0	39.0	35.0	39.0
12-13	37.50241223374104	39.0	38.0	39.0	35.0	39.0
14-15	38.958947938579364	41.0	39.0	41.0	36.0	41.0
16-17	38.79055260936929	41.0	39.0	41.0	35.5	41.0
18-19	38.86309597289228	41.0	39.0	41.0	35.5	41.0
20-21	38.931108997694835	41.0	39.0	41.0	36.0	41.0
22-23	38.880938944663484	41.0	39.0	41.0	35.5	41.0
24-25	38.822267499716574	41.0	39.0	41.0	35.0	41.0
26-27	38.64340194238351	41.0	39.0	41.0	35.0	41.0
28-29	38.55647587136433	41.0	39.0	41.0	34.0	41.0
30-31	38.48032423444644	40.0	39.0	41.0	34.0	41.0
32-33	38.29685653822414	40.0	38.0	41.0	34.0	41.0
34-35	38.281249448902216	40.0	38.0	41.0	34.0	41.0
36-37	38.29830009951252	40.0	38.0	41.0	34.0	41.0
38-39	38.19397382442969	40.0	38.0	41.0	33.5	41.0
40-41	38.1049655485155	40.0	38.0	41.0	33.0	41.0
42-43	37.95083514933175	40.0	38.0	41.0	33.0	41.0
44-45	37.79860682479499	40.0	38.0	41.0	33.0	41.0
46-47	37.62276758159396	40.0	37.0	41.0	32.0	41.0
48-49	37.42878430977364	40.0	37.0	41.0	32.0	41.0
50-51	36.17343078841624	38.5	35.0	40.0	29.5	40.5
52-53	36.558619799211456	39.0	35.5	40.0	30.5	40.5
54-55	37.04928010883394	39.5	36.0	41.0	31.0	41.0
56-57	36.90942660637132	39.0	35.0	41.0	31.0	41.0
58-59	36.766398780656786	39.0	35.0	41.0	31.0	41.0
60-61	36.52510549586204	39.0	35.0	41.0	31.0	41.0
62-63	36.233834254978774	38.5	35.0	41.0	30.0	41.0
64-65	35.97358824492675	37.5	35.0	40.5	30.0	41.0
66-67	35.72342008137352	37.0	35.0	40.0	29.5	41.0
68-69	35.40033947623667	36.5	35.0	40.0	29.0	41.0
70-71	35.090720143096476	36.0	34.5	39.0	29.0	41.0
72-73	34.770253316034115	35.5	34.0	39.0	29.0	41.0
74-75	34.4862401904594	35.0	34.0	38.5	29.0	40.5
76-77	34.12754229281872	35.0	34.0	37.0	28.0	39.5
78-79	33.88134896141686	35.0	34.0	37.0	28.0	39.0
80-81	33.55559537455755	35.0	34.0	36.5	27.0	39.0
82-83	33.27952057641679	35.0	34.0	36.0	27.0	38.0
84-85	33.04605351002053	35.0	33.5	36.0	27.0	37.0
86-87	32.808526584957235	35.0	33.0	35.0	26.0	37.0
88-89	32.63286180356985	35.0	33.0	35.0	26.0	36.0
90-91	32.44415332485167	35.0	33.0	35.0	25.5	36.0
92-93	32.298103593787395	35.0	33.0	35.0	25.0	36.0
94-95	32.15166211092496	35.0	33.0	35.0	25.0	35.5
96-97	32.01758726239812	35.0	33.0	35.0	25.0	35.0
98-99	31.946718606320935	35.0	33.0	35.0	25.0	35.0
100-101	30.739187776336173	34.0	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	81.0
8	301.0
9	310.0
10	439.0
11	1025.0
12	1771.0
13	1993.0
14	2142.0
15	2137.0
16	2199.0
17	2283.0
18	2546.0
19	2809.0
20	3173.0
21	3802.0
22	4256.0
23	4421.0
24	4399.0
25	4619.0
26	4936.0
27	5607.0
28	6018.0
29	7046.0
30	8711.0
31	10734.0
32	14014.0
33	19752.0
34	31195.0
35	53545.0
36	109488.0
37	186957.0
38	222798.0
39	68350.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13391765571241	19.082284989004528	13.662268057365704	24.12152929791736
2	15.771367820131532	19.783616692426584	39.318703715600435	25.126311771841447
3	19.977502557100173	24.89973144149909	28.115883245073487	27.00688275632725
4	12.728532379356821	16.546033985413228	38.34179399649817	32.38363963873178
5	14.161638555430988	38.24228148185471	34.03340597327018	13.562673989444116
6	31.136836005895173	36.57324247042967	18.60997392520186	13.679947598473301
7	27.860984795999343	30.75226422462116	22.006625769962337	19.38012520941716
8	25.64361923236802	35.09567057578697	20.4832025394586	18.77750765238641
9	25.99027548591079	16.188922619572473	20.015871616259588	37.804930278257146
10-11	25.743824555657728	25.326690768009875	27.824580850769014	21.10490382556338
12-13	26.249448902213206	22.768463350422614	27.941854459798204	23.040233287565975
14-15	23.238640122743067	24.86215033505455	25.011672563332716	26.88753697886967
16-17	23.389444820902344	27.54608632617363	26.042580014813183	23.021888838110847
18-19	23.28855068793149	27.11536256427897	26.760906791866546	22.83517995592299
20-21	24.209235546576917	25.678822062323526	26.699000678648776	23.41294171245078
22-23	24.09170239895321	26.267847497143205	26.075104297517846	23.565345806385736
24-25	23.526239730074675	26.1963258204286	26.495871871145432	23.78156257835129
26-27	23.53874059984632	26.8793379268646	26.530792195195684	23.05112927809339
28-29	23.255003967904063	26.85918349351909	26.681068688828148	23.2047438497487
30-31	23.440298789474348	26.276090543791803	26.660410394649	23.623200272084848
32-33	23.28977036542507	26.347008956126317	26.796137906710165	23.567082771738445
34-35	23.924005189766586	26.039212969377857	26.49715948455037	23.53962235630519
36-37	23.267709094574503	26.176637351006725	26.995690849986143	23.55996270443263
38-39	23.381140442042543	26.461644085838643	26.425804501048734	23.731410971070087
40-41	23.929474840517198	26.09301147873059	26.67507331673839	23.302440364013826
42-43	23.795747972180546	26.176916303555032	26.681871375969507	23.345464348294918
44-45	23.876045656117718	26.006160552308	26.47040667204193	23.647387119532354
46-47	23.676924433471473	26.00224218070969	26.473603990577804	23.847229395241033
48-49	23.254311159257814	26.251527328151962	26.602277450968042	23.89188406162218
50-51	23.354138586922293	26.420761585650045	26.409046821267967	23.8160530061597
52-53	23.63730837542671	26.371824102182977	25.945746784738056	24.045120737652258
54-55	23.71981558693489	25.73462909544384	26.419061055336517	24.126494262284755
56-57	23.64738559209946	26.258014536385048	26.231057981785433	23.863541889730055
58-59	23.81392746991352	26.30822011006511	26.548420585804422	23.329431834216948
60-61	23.66779325746432	26.497919534328474	26.17424254355434	23.660044664652872
62-63	23.563055811946157	26.10904073512873	26.583018576680388	23.744884876244722
64-65	24.18940439001857	25.65150454334341	26.102782470930986	24.05630859570703
66-67	23.29187303371211	26.123579579458507	26.06291365279435	24.521633734035035
68-69	23.583458248831672	26.25511733659163	26.744429188658092	23.4169952259186
70-71	23.683348659100357	25.923765855870606	26.540176603222186	23.852708881806844
72-73	23.54125990401451	26.185962437174855	26.637232796301664	23.635544862508976
74-75	23.615075516142443	25.917467595450137	26.49709650194616	23.97036038646126
76-77	23.582135614143375	25.59115472306549	26.264375779409725	24.56233388338141
78-79	23.605932382232083	25.447974484431686	26.61848795185656	24.32760518147967
80-81	23.499849370444085	26.17342945053615	26.154584999817228	24.172136179202532
82-83	23.34186816365448	26.33494051981591	26.39569053037154	23.927500786158074
84-85	23.654516322725804	25.664102100440857	26.788449433982947	23.89293214285039
86-87	23.393825260390077	26.508737232598328	26.258714858526538	23.838722648485057
88-89	23.613087936106496	25.889256216051376	26.00644807235148	24.491207775490647
90-91	23.304382329600564	25.778213057553508	26.40973962991422	24.507664982931715
92-93	23.278916303505056	25.87132017406563	26.757583563911297	24.092179958518013
94-95	23.524128635670827	25.72102485293562	26.62784838827516	24.126998123118394
96-97	23.759557610188068	26.095141521911646	26.492939650068653	23.652361217831636
98-99	23.002946980179843	26.158086371271633	26.556706537081	24.282260111467526
100-101	23.542760133519273	25.673913769537464	26.26135320284305	24.521972894100212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	2.0
3	4.0
4	4.5
5	3.5
6	3.0
7	11.0
8	15.5
9	16.5
10	22.5
11	23.0
12	29.5
13	29.5
14	28.5
15	38.0
16	39.5
17	38.5
18	52.5
19	69.5
20	87.0
21	106.0
22	121.0
23	164.0
24	224.5
25	292.5
26	416.0
27	581.5
28	810.0
29	1291.0
30	2258.0
31	4046.0
32	6788.5
33	10801.0
34	15458.5
35	19768.0
36	24022.0
37	26830.5
38	27535.5
39	26925.0
40	25321.5
41	23805.0
42	22887.0
43	22654.5
44	23105.0
45	24436.0
46	26441.5
47	27667.5
48	28831.0
49	30998.0
50	33112.0
51	33198.0
52	32708.0
53	33206.0
54	34119.5
55	33902.0
56	29559.5
57	25240.0
58	23246.0
59	21148.0
60	17580.5
61	13595.5
62	10940.0
63	8056.0
64	5141.5
65	3405.5
66	2626.0
67	2213.5
68	1762.5
69	1209.5
70	800.0
71	537.0
72	379.0
73	292.0
74	220.5
75	162.5
76	106.5
77	70.0
78	50.5
79	35.5
80	27.0
81	18.5
82	16.0
83	14.5
84	7.0
85	7.0
86	7.5
87	4.0
88	2.0
89	3.0
90	5.5
91	5.0
92	3.0
93	2.0
94	4.5
95	4.5
96	2.5
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16010177988839483
2	1.259652084094373E-4
3	2.519304168188746E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.04314308388023228
16-17	0.08282212452920504
18-19	0.032624988978044264
20-21	0.048055727008200336
22-23	0.07387859473213498
24-25	0.021288120221194907
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.026452693765981833
38-39	0.0069280864625190525
40-41	0.006802121254109615
42-43	0.00466071271114918
44-45	0.013856172925038105
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.019902502928691095
60-61	0.022358824492675122
62-63	0.03369569324952448
64-65	0.057503117638908134
66-67	0.022547772305289277
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.019965485532895816
80-81	0.06713945608223008
82-83	0.05725118722208926
84-85	0.037600614710217035
86-87	0.06814717774950559
88-89	0.03772657991862648
90-91	0.0
92-93	0.0039679040648972754
94-95	0.0
96-97	0.0
98-99	8.187738546613425E-4
100-101	0.053724161386625016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	793870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.425004455500165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.427284855644	45.236894626682805
2	13.32874003966849	15.5746339240767
3	4.354880039403259	7.633016571159123
4	1.801760934988782	4.2107156261786285
5	0.9724194880210699	2.8406806460123097
6	0.5385404192205977	1.8878535835458203
7	0.3355806125876593	1.3724409149929429
8	0.2162569820978481	1.0107852114079834
9	0.1563086340807314	0.8219099378339888
>10	0.7328230198549915	7.748412919260142
>50	0.07063742387663272	2.8862125922495445
>100	0.060855416632526485	6.851533061695204
>500	0.0032601116028224287	1.3133681714539693
>1k	6.520223205644857E-4	0.6115422134508669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2044	0.2574728859888899	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1570	0.1977653772028166	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1202	0.15141018050814364	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	999	0.12583924320102788	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	925	0.11651781777872951	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	843	0.10618867068915565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	1.2596520840943732E-4	0.0
20-21	0.0	0.0	0.0	1.2596520840943732E-4	0.0
22-23	0.0	0.0	0.0	3.77895625228312E-4	0.0
24-25	0.0	0.0	0.0	6.298260420471866E-4	0.0
26-27	0.0	0.0	0.0	8.817564588660612E-4	0.0
28-29	0.0	0.0	0.0	0.0012596520840943732	0.0
30-31	0.0	0.0	0.0	0.0032121128144406517	0.0
32-33	0.0	0.0	0.0	0.010958973131621046	0.0
34-35	0.0	0.0	0.0	0.02015443334550997	0.0
36-37	0.0	0.0	0.0	0.034892362729414136	0.0
38-39	0.0	0.0	0.0	0.06273067378789979	0.0
40-41	0.0	0.0	0.0	0.09541864537014877	0.0
42-43	0.0	0.0	0.0	0.12609117361784675	0.0
44-45	0.0	0.0	0.0	0.1623691536397647	0.0
46-47	0.0	0.0	0.0	0.2004736291836195	0.0
48-49	0.0	0.0	0.0	0.24859233879602455	0.0
50-51	0.0	0.0	0.0	0.3009938654943505	0.0
52-53	0.0	0.0	0.0	0.35805610490382556	0.0
54-55	0.0	0.0	0.0	0.41795256150251303	0.0
56-57	0.0	0.0	0.0	0.48421026112587706	0.0
58-59	0.0	0.0	0.0	0.5590965775252875	0.0
60-61	0.0	0.0	0.0	0.6422965976797208	0.0
62-63	0.0	0.0	0.0	0.730031365336894	0.0
64-65	0.0	0.0	0.0	0.8180180634108859	0.0
66-67	0.0	0.0	0.0	0.9228840994117424	0.0
68-69	0.0	0.0	0.0	1.046392986257196	0.0
70-71	0.0	0.0	0.0	1.1810497940468843	0.0
72-73	0.0	0.0	0.0	1.3187297668383993	0.0
74-75	0.0	0.0	0.0	1.459369922027536	0.0
76-77	0.0	0.0	0.0	1.618086084623427	0.0
78-79	0.0	0.0	0.0	1.7954450980639147	0.0
80-81	0.0	0.0	0.0	1.9960446924559436	0.0
82-83	0.0	0.0	0.0	2.2168617027976874	0.0
84-85	0.0	0.0	0.0	2.4479448776248	0.0
86-87	0.0	0.0	0.0	2.6927582601685414	0.0
88-89	0.0	0.0	0.0	2.965346971166564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	985	0.0	45.386093	1
GGTATCA	520	0.0	40.242085	1
AAACCGT	170	0.0	36.30896	6
ATCAACG	1240	0.0	35.2278	3
TCAACGC	1285	0.0	33.62464	4
CGTCCGA	30	0.0039396756	31.685898	16-17
CCGTGCG	185	9.094947E-12	30.821747	9
ACCGTGC	205	1.8189894E-12	30.11366	8
AACCGTG	205	1.8189894E-12	30.109867	7
TCGCACC	825	0.0	29.976475	1
CGCACCA	865	0.0	28.543459	2
ACGCAGA	1660	0.0	26.8868	7
CTGTGCG	990	0.0	26.878223	9
CGCAGAG	1630	0.0	26.802439	8
CAACGCA	1660	0.0	26.314741	5
TATCAAC	1715	0.0	26.3014	2
GTACATG	2660	0.0	25.924927	1
AACGCAG	1725	0.0	25.873676	6
TACATGG	2615	0.0	24.512152	2
TGTTCCG	550	0.0	24.172121	5
>>END_MODULE