FastQCFastQC Report
Fri 10 Feb 2017
SRR3224482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793870
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG11180.14082910300175092No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10080.12697293007671281No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9970.125587312784209No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA9940.12520941715898068No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT9820.12369783465806744No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG8130.10240971443687254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.043.6405261
GTATCAA12300.039.9512021
AACCGTG1750.037.9914867
AAACCGT1502.5465852E-1134.8255276
ATCAACG13550.034.697023
CCGTGCG1605.2750693E-1132.6489339
ACCGTGC2050.032.4317558
TCAACGC14450.032.2073144
GTCTAAT3750.031.8058091
TATAACG1201.5077785E-731.659572
CAACGCA16150.029.1111225
AACGCAG16750.028.0683366
ATAACGC1205.3883086E-627.7021263
CGCAGAG17600.027.2524158
GTTATTA3500.027.1367748
ATACCGT1457.8978337E-726.2010256
ACGCAGA18100.025.712477
TATCAAC19700.024.1062742
TCGCACC5750.024.0617851
GTTCCGG6000.022.9531886
Produced by FastQC (version 0.11.5)