##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793870
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16747074458035	34.0	33.0	34.0	31.0	34.0
2	33.30067643316916	34.0	34.0	34.0	31.0	34.0
3	33.41753309735851	34.0	34.0	34.0	31.0	34.0
4	36.62492095683172	37.0	37.0	37.0	35.0	37.0
5	36.653447037928125	37.0	37.0	37.0	35.0	37.0
6	36.69104387368209	37.0	37.0	37.0	36.0	37.0
7	36.66992832579641	37.0	37.0	37.0	36.0	37.0
8	36.651232569564286	37.0	37.0	37.0	35.0	37.0
9	38.492979958935344	39.0	39.0	39.0	37.0	39.0
10-11	38.5211728620555	39.0	39.0	39.0	37.5	39.0
12-13	38.51911333089801	39.0	39.0	39.0	37.0	39.0
14-15	40.133547054303605	41.0	40.0	41.0	38.0	41.0
16-17	40.14561955987756	41.0	40.0	41.0	38.0	41.0
18-19	40.12876226586217	41.0	40.0	41.0	38.0	41.0
20-21	40.12833335432754	41.0	40.0	41.0	38.0	41.0
22-23	40.054570017761094	41.0	40.0	41.0	38.0	41.0
24-25	40.028850441508055	41.0	40.0	41.0	38.0	41.0
26-27	39.97469862823888	41.0	40.0	41.0	38.0	41.0
28-29	39.9425119981861	41.0	40.0	41.0	38.0	41.0
30-31	39.89300893093328	41.0	40.0	41.0	38.0	41.0
32-33	39.85479549548415	41.0	40.0	41.0	38.0	41.0
34-35	39.78217151422777	41.0	40.0	41.0	38.0	41.0
36-37	39.68866754002544	41.0	40.0	41.0	37.0	41.0
38-39	39.59815397987076	41.0	40.0	41.0	37.0	41.0
40-41	39.477801781148045	41.0	39.5	41.0	36.5	41.0
42-43	39.389735095166714	41.0	39.0	41.0	36.0	41.0
44-45	39.17360902918614	41.0	39.0	41.0	35.0	41.0
46-47	38.969981861009984	41.0	39.0	41.0	35.0	41.0
48-49	38.890577172584926	40.5	39.0	41.0	35.0	41.0
50-51	38.759175305780545	40.0	38.0	41.0	35.0	41.0
52-53	38.53977477420736	40.0	37.5	41.0	35.0	41.0
54-55	38.365021980928866	40.0	37.0	41.0	35.0	41.0
56-57	38.151971355511606	40.0	36.5	41.0	35.0	41.0
58-59	37.89516230617103	40.0	36.0	41.0	34.0	41.0
60-61	37.653304067416585	39.0	35.0	41.0	34.0	41.0
62-63	37.430593170166404	39.0	35.0	41.0	34.0	41.0
64-65	37.15940141332963	38.5	35.0	41.0	33.5	41.0
66-67	36.869322433143964	37.5	35.0	40.5	33.0	41.0
68-69	36.585027775328456	37.0	35.0	40.0	33.0	41.0
70-71	36.275427966795576	36.5	35.0	39.5	33.0	41.0
72-73	35.96117689294216	36.0	35.0	39.0	33.0	41.0
74-75	35.630248655321395	35.5	35.0	39.0	32.5	41.0
76-77	33.792432640104806	34.5	32.5	36.5	29.5	39.0
78-79	35.050598964565985	35.0	35.0	37.0	32.5	39.0
80-81	34.953345006109316	35.0	35.0	37.0	33.0	39.0
82-83	34.72777280915011	35.0	35.0	36.0	33.0	38.5
84-85	34.51061319863454	35.0	35.0	36.0	33.0	37.0
86-87	34.30233539496391	35.0	35.0	36.0	32.0	37.0
88-89	34.13246753246753	35.0	35.0	35.0	32.0	36.5
90-91	33.98989255167723	35.0	35.0	35.0	32.0	36.0
92-93	33.89055764797763	35.0	34.5	35.0	32.0	36.0
94-95	33.78316286041795	35.0	34.0	35.0	32.0	36.0
96-97	33.733011072341824	35.0	34.0	35.0	32.0	36.0
98-99	33.686856790154565	35.0	34.0	35.0	31.0	35.0
100-101	32.870631211659344	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	14.0
15	27.0
16	100.0
17	178.0
18	333.0
19	512.0
20	728.0
21	1063.0
22	1416.0
23	1489.0
24	1561.0
25	1984.0
26	2445.0
27	3345.0
28	4187.0
29	5121.0
30	5760.0
31	6472.0
32	7994.0
33	11064.0
34	18098.0
35	35686.0
36	95088.0
37	209537.0
38	278993.0
39	100641.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.689954425163485	18.94551396037862	13.583731264135352	23.780800350322544
2	15.71768677491277	19.91749278849182	39.697809465025756	24.667010971569653
3	19.731316210462673	25.327068663635107	28.246690264149045	26.694924861753183
4	12.824139972539584	16.714449469056646	38.59019738748158	31.871213170922193
5	14.055827780367064	37.663345384004934	34.54507664982931	13.73575018579868
6	31.10181767795735	36.35544862508975	18.93017748497865	13.612556211974253
7	28.13055034199554	30.585108393061837	22.170128610477786	19.114212654464836
8	25.700807436985905	34.75808381724968	20.43017118671823	19.110937559046192
9	25.916711804199682	16.392734326778942	20.157708440928616	37.532845428092756
10-11	25.825575975915456	25.314661090606776	27.9552067718896	20.904556161588168
12-13	26.42246211596357	22.966228727625428	27.893042941539548	22.718266214871452
14-15	23.34557295275045	25.100709184123343	25.2095431241891	26.344174738937102
16-17	23.332913449305302	27.631098290652123	26.0571630115762	22.97882524846637
18-19	23.178732034212153	27.08107120813231	27.072631539168885	22.667565218486654
20-21	24.151268295079365	25.936908350454296	26.908614984235275	23.00320837023106
22-23	24.000576929375278	26.233847866998882	26.480743407511163	23.284831796114673
24-25	23.80353144573874	26.03312265585882	26.52753765584307	23.63580824255937
26-27	23.684220471250686	26.932713564990646	26.40622539664544	22.97684056711323
28-29	23.311504713508025	26.755228255728042	26.854493153808313	23.078773876955616
30-31	23.54670625380656	26.14231341652595	26.8171800965031	23.493800233164393
32-33	23.390019883645717	26.38579311309115	27.005291178576808	23.21889582468633
34-35	23.97314545143053	26.157990607584473	26.44526801640649	23.4235959245785
36-37	23.22931080852125	26.165442989580534	27.00117969315381	23.604066508744403
38-39	23.381787950168164	26.373902528121736	26.52336024789953	23.720949273810575
40-41	23.718933830476022	26.295363220678446	26.712874903951526	23.272828044894002
42-43	23.60613198634537	26.276972300250673	26.84652399007394	23.270371723330015
44-45	23.79981456146053	26.06431895428789	26.651436014885476	23.484430469366107
46-47	23.6778143090546	25.903329668999735	26.577974665671583	23.84088135627408
48-49	23.298589744962452	25.913585899842985	26.997840308818834	23.789984046375732
50-51	23.652676130852658	26.093692922014938	26.582123017622532	23.67150792950987
52-53	23.664642825651555	26.3400808696638	26.322256792673866	23.673019512010782
54-55	23.86769874160757	25.786526761308526	26.55056873291597	23.79520576416794
56-57	23.801710155425727	26.073728800214163	26.46155299749618	23.66300804686393
58-59	23.945824792580208	26.185649770415736	26.565045094546374	23.303480342457682
60-61	23.702461822658545	26.37144200030494	26.43085487758931	23.495241299447205
62-63	23.585851587791453	25.96042173151775	26.72666809427236	23.72705858641843
64-65	23.784372756244725	25.822804741330447	26.454142365878546	23.938680136546285
66-67	23.521987227127866	26.0945116958696	26.341214556539484	24.042286520463048
68-69	23.82953128345951	26.03656770000126	26.665323037776968	23.468577978762266
70-71	23.941073475506062	25.939196593900764	26.495584919445246	23.62414501114792
72-73	23.550392381624196	26.073601471273633	26.61600765868467	23.7599984884175
74-75	23.72888508194037	25.8088855858012	26.52581656946351	23.936412762794916
76-77	23.693296618581428	25.542281588374895	26.460822202179706	24.30359959086397
78-79	23.72067095408764	25.56491285533419	26.358631636631625	24.355784553946545
80-81	23.38984331942367	26.124368159987405	26.382426580584205	24.103361940004724
82-83	23.308492775201767	26.165219943491508	26.668518371557816	23.857768909748913
84-85	23.61352201812442	25.721172620382294	26.764259062840907	23.901046298652386
86-87	23.27629208812526	26.459621852444354	26.47480066005769	23.78928539937269
88-89	23.624522906773148	25.78658974391273	26.017358005718823	24.5715293435953
90-91	23.552344842354543	25.896242457833146	26.1813017244637	24.37011097534861
92-93	23.617846750727452	25.953493645055236	26.59383778200461	23.834821822212703
94-95	23.47349062189023	25.849761295930062	26.36873795457695	24.308010127602756
96-97	23.619925176666204	26.164107473515813	26.400796100117148	23.815171249700835
98-99	22.67770541776361	26.321438018819205	26.624951188481745	24.375905374935442
100-101	23.255824213779196	26.079015056639665	26.230785278540587	24.43437545104055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	1.0
3	1.5
4	2.0
5	0.5
6	1.5
7	6.0
8	10.0
9	15.0
10	17.5
11	18.0
12	21.0
13	26.5
14	34.0
15	36.5
16	36.5
17	40.5
18	52.5
19	60.0
20	67.0
21	89.5
22	110.5
23	138.5
24	184.0
25	279.5
26	408.0
27	546.0
28	779.5
29	1240.5
30	2223.5
31	4069.0
32	6880.0
33	10874.5
34	15359.0
35	19841.0
36	24014.0
37	26528.5
38	27263.5
39	26899.5
40	25434.5
41	23861.0
42	23085.5
43	23064.0
44	23880.0
45	25090.5
46	26294.0
47	27699.5
48	29229.5
49	31854.0
50	34646.5
51	34069.0
52	32168.5
53	32788.0
54	34420.5
55	33710.0
56	29295.0
57	25177.5
58	23380.0
59	21539.5
60	17685.0
61	13216.5
62	10470.5
63	7506.0
64	4577.5
65	2963.0
66	2237.0
67	1861.5
68	1452.0
69	985.0
70	630.0
71	421.0
72	303.0
73	232.0
74	163.0
75	113.5
76	68.0
77	39.0
78	26.5
79	15.5
80	14.0
81	11.0
82	6.0
83	4.0
84	2.0
85	0.5
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47123584465970497
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0011336868756849359
22-23	0.0015115825009132478
24-25	3.149130210235933E-4
26-27	0.0031491302102359332
28-29	0.004345799690125588
30-31	0.0011966694798896545
32-33	1.8894781261415597E-4
34-35	0.004786677919558618
36-37	0.0024563215639840275
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009573355839117236
46-47	0.08036580296522101
48-49	8.187738546613425E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.010392129693778578
58-59	0.046670109715696524
60-61	0.03413657147895751
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.519304168188746E-4
78-79	0.009447390630707798
80-81	0.007242999483542646
82-83	8.817564588660612E-4
84-85	0.0030231650018264955
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0712333253555368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	793870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.268385045617485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61402935405141	42.546793185443875
2	14.382749975720094	16.185882272973277
3	4.683506079468803	7.905999703291229
4	1.98278375166046	4.46272158402499
5	1.0204882757894107	2.8710613618328424
6	0.5853171324465423	1.976090986937924
7	0.34994663837813533	1.3783652535576265
8	0.24620338975665695	1.1082773707491047
9	0.17253736362481464	0.8737558930077111
>10	0.8139502233957031	8.287665302553867
>50	0.07323675125070829	2.8847546893116927
>100	0.07032391143051885	7.51630824529791
>500	0.004479230024301476	1.7344066518007406
>1k	4.479230024301476E-4	0.26791749921734903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1118	0.14082910300175092	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1008	0.12697293007671281	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	997	0.125587312784209	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	994	0.12520941715898068	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	982	0.12369783465806744	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	813	0.10240971443687254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.2596520840943732E-4	0.0
16-17	0.0	0.0	0.0	1.2596520840943732E-4	0.0
18-19	0.0	0.0	0.0	2.5193041681887464E-4	0.0
20-21	0.0	0.0	0.0	2.5193041681887464E-4	0.0
22-23	0.0	0.0	0.0	4.4087822943303064E-4	0.0
24-25	0.0	0.0	0.0	5.668434378424679E-4	0.0
26-27	0.0	0.0	0.0	8.817564588660612E-4	0.0
28-29	0.0	0.0	0.0	0.0013226346882990918	0.0
30-31	0.0	0.0	0.0	0.0032750954186453705	0.0
32-33	0.0	0.0	0.0	0.010958973131621048	0.0
34-35	0.0	0.0	0.0	0.020406363762328843	0.0
36-37	0.0	0.0	0.0	0.03533324095884716	0.0
38-39	0.0	0.0	0.0	0.06304558680892337	0.0
40-41	0.0	0.0	0.0	0.09611145401640067	0.0
42-43	0.0	0.0	0.0	0.12728784309773641	0.0
44-45	0.0	0.0	0.0	0.16432161437011097	0.0
46-47	0.0	0.0	0.0	0.20267802033078464	0.0
48-49	0.0	0.0	0.0	0.2508597125473944	0.0
50-51	0.0	0.0	0.0	0.3031982566415156	0.0
52-53	0.0	0.0	0.0	0.36038646125940016	0.0
54-55	0.0	0.0	0.0	0.42135362212956784	0.0
56-57	0.0	0.0	0.0	0.48861904342020734	0.0
58-59	0.0	0.0	0.0	0.5643241336742791	0.0
60-61	0.0	0.0	0.0	0.6479650320581456	0.0
62-63	0.0	0.0	0.0	0.7361406779447517	0.0
64-65	0.0	0.0	0.0	0.824694219456586	0.0
66-67	0.0	0.0	0.0	0.9302530641036946	0.0
68-69	0.0	0.0	0.0	1.0553365160542658	0.0
70-71	0.0	0.0	0.0	1.1913159585322535	0.0
72-73	0.0	0.0	0.0	1.3306334790330911	0.0
74-75	0.0	0.0	0.0	1.4742967992240543	0.0
76-77	0.0	0.0	0.0	1.6358471790091578	0.0
78-79	0.0	0.0	0.0	1.8150326879715823	0.0
80-81	0.0	0.0	0.0	2.0172698300729337	0.0
82-83	0.0	0.0	0.0	2.240858074999685	0.0
84-85	0.0	0.0	0.0	2.4735787975361205	0.0
86-87	0.0	0.0	0.0	2.723745701437263	0.0
88-89	0.0	0.0	0.0	3.000239333895978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	645	0.0	43.640526	1
GTATCAA	1230	0.0	39.951202	1
AACCGTG	175	0.0	37.991486	7
AAACCGT	150	2.5465852E-11	34.825527	6
ATCAACG	1355	0.0	34.69702	3
CCGTGCG	160	5.2750693E-11	32.648933	9
ACCGTGC	205	0.0	32.431755	8
TCAACGC	1445	0.0	32.207314	4
GTCTAAT	375	0.0	31.805809	1
TATAACG	120	1.5077785E-7	31.65957	2
CAACGCA	1615	0.0	29.111122	5
AACGCAG	1675	0.0	28.068336	6
ATAACGC	120	5.3883086E-6	27.702126	3
CGCAGAG	1760	0.0	27.252415	8
GTTATTA	350	0.0	27.136774	8
ATACCGT	145	7.8978337E-7	26.201025	6
ACGCAGA	1810	0.0	25.71247	7
TATCAAC	1970	0.0	24.106274	2
TCGCACC	575	0.0	24.061785	1
GTTCCGG	600	0.0	22.953188	6
>>END_MODULE