FastQCFastQC Report
Fri 10 Feb 2017
SRR3224481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059117
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18290.17269102469321143No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13980.1319967482346143No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG13580.128220017240777No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA12800.12085539180279421No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC12630.11925028113041335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.045.0148431
GTATCAA15600.042.060741
AAACCGT2050.037.0537346
ATCAACG17100.036.647443
TCAACGC18500.033.8741234
CCGTGCG1850.033.3876769
CGATTCG300.00395087931.66800554-55
AACCGTG2400.031.6500667
CCCATAA9450.030.64532
TATCAAC21450.030.100762
CGCAGAG21250.029.4959858
GCCCATA9550.029.374541
CATAATA9700.028.8766064
ACGCAGA21950.028.5499427
ACCGTGC2500.028.4904388
CCATAAT10450.028.1670443
GACGTGC950.001228892124.9916138
GCAGAGT25050.024.467899
GTACATG35300.024.1101971
ATAATAA12750.023.8306395
Produced by FastQC (version 0.11.5)