##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224481_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059117
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28835435556223	34.0	31.0	34.0	31.0	34.0
2	32.46157884350832	34.0	31.0	34.0	31.0	34.0
3	32.6016219171253	34.0	31.0	34.0	31.0	34.0
4	35.88057882179211	37.0	37.0	37.0	35.0	37.0
5	35.90203254220261	37.0	37.0	37.0	35.0	37.0
6	35.99851196798843	37.0	37.0	37.0	35.0	37.0
7	35.96095426662021	37.0	37.0	37.0	35.0	37.0
8	35.99971296844447	37.0	37.0	37.0	35.0	37.0
9	37.731463096145184	39.0	39.0	39.0	35.0	39.0
10-11	37.80637266704245	39.0	39.0	39.0	36.0	39.0
12-13	37.812531571110654	39.0	39.0	39.0	36.0	39.0
14-15	39.32894996492361	41.0	40.0	41.0	37.0	41.0
16-17	39.16721051592978	41.0	40.0	41.0	36.5	41.0
18-19	39.25447660645614	41.0	40.0	41.0	36.5	41.0
20-21	39.32510666904601	41.0	40.0	41.0	37.0	41.0
22-23	39.28648298535478	41.0	40.0	41.0	37.0	41.0
24-25	39.22778314388306	41.0	40.0	41.0	36.0	41.0
26-27	39.07434589379643	41.0	39.5	41.0	36.0	41.0
28-29	39.002736241605035	41.0	39.0	41.0	35.5	41.0
30-31	38.93788740998398	41.0	39.0	41.0	35.0	41.0
32-33	38.75474050553433	41.0	39.0	41.0	35.0	41.0
34-35	38.76507600199034	41.0	39.0	41.0	35.0	41.0
36-37	38.76503020912703	41.0	39.0	41.0	35.0	41.0
38-39	38.668800047586814	41.0	39.0	41.0	35.0	41.0
40-41	38.59001177395887	41.0	39.0	41.0	35.0	41.0
42-43	38.45976837308815	40.0	38.5	41.0	34.5	41.0
44-45	38.30679660509651	40.0	38.0	41.0	34.0	41.0
46-47	38.164457279035275	40.0	38.0	41.0	34.0	41.0
48-49	37.98346358334348	40.0	38.0	41.0	33.0	41.0
50-51	36.7237467626334	39.0	36.0	40.0	31.5	40.5
52-53	37.059315448623714	39.5	36.5	40.0	32.5	40.5
54-55	37.54218986193216	40.0	36.5	41.0	33.0	41.0
56-57	37.424838332308894	40.0	36.0	41.0	33.0	41.0
58-59	37.30656764078	40.0	35.5	41.0	33.0	41.0
60-61	37.088577560363966	39.5	35.0	41.0	32.5	41.0
62-63	36.83230039740651	39.0	35.0	41.0	32.0	41.0
64-65	36.60422738941968	39.0	35.0	41.0	32.0	41.0
66-67	36.386062163103794	38.0	35.0	41.0	31.0	41.0
68-69	36.105315560037276	37.0	35.0	40.5	31.0	41.0
70-71	35.821029215846785	37.0	35.0	40.0	31.0	41.0
72-73	35.513360657982076	36.0	35.0	39.5	30.5	41.0
74-75	35.22697728390725	36.0	35.0	39.0	30.0	41.0
76-77	34.86115226174256	35.5	35.0	39.0	30.0	40.5
78-79	34.591027714596216	35.0	34.5	37.5	30.0	39.5
80-81	34.25726949902607	35.0	34.0	37.0	29.5	39.0
82-83	33.94738116751974	35.0	34.0	37.0	29.0	39.0
84-85	33.67674865005472	35.0	34.0	36.0	29.0	37.0
86-87	33.41827342965885	35.0	34.0	36.0	29.0	37.0
88-89	33.22987025984853	35.0	34.0	35.5	29.0	37.0
90-91	33.03168110794181	35.0	34.0	35.0	29.0	36.0
92-93	32.87978145946104	35.0	34.0	35.0	28.5	36.0
94-95	32.7462537188998	35.0	34.0	35.0	27.0	36.0
96-97	32.6063739888983	35.0	33.5	35.0	27.0	36.0
98-99	32.556932803457975	35.0	33.0	35.0	27.0	35.0
100-101	31.429628643483202	34.5	31.0	35.0	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	157.0
8	623.0
9	479.0
10	517.0
11	895.0
12	1530.0
13	1792.0
14	1841.0
15	1939.0
16	2056.0
17	2326.0
18	2537.0
19	2849.0
20	3325.0
21	4227.0
22	5043.0
23	5069.0
24	5033.0
25	5353.0
26	5662.0
27	5843.0
28	6407.0
29	7300.0
30	9076.0
31	11206.0
32	14970.0
33	21054.0
34	33966.0
35	60778.0
36	127988.0
37	233350.0
38	337136.0
39	136766.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07904416979509	21.206135166570526	14.46293652069484	23.251884142939545
2	14.967387897076428	21.37896132246137	39.79195857677535	23.86169220368685
3	18.24959352817544	28.092820980555445	30.101386630712085	23.556198860557032
4	12.379180015050274	18.466420612642416	41.0463622054976	28.108037166809712
5	12.953620799212928	39.15752461720471	35.39221823462375	12.496636348958614
6	27.23211883106399	39.4851560309201	20.437779773150652	12.844945364865259
7	24.83993741956743	32.82186953849291	24.592467121196243	17.74572592074341
8	24.523447362283864	35.265792164605045	22.24173533235705	17.969025140754045
9	25.922537358950898	16.47787732611222	21.89153795095348	35.70804736398339
10-11	24.52047318662622	26.205084046427352	29.2753775078674	19.999065259079025
12-13	24.98123436787437	23.99947314602636	29.067090793557277	21.952201692541994
14-15	22.588972492627736	25.68045844990734	26.318978594462255	25.41159046300267
16-17	22.330781918007297	28.455123610804673	27.080042880029936	22.134051591158098
18-19	22.17257070107969	28.081248630556168	27.841359848284515	21.904820820079635
20-21	23.49369793243443	26.64551100187454	27.388053110510878	22.472737955180154
22-23	23.45036524936779	27.120196638940502	26.878791443390483	22.55064666830123
24-25	22.69663685324373	27.101789655299413	27.33381873401082	22.867754757446036
26-27	22.654343193433775	27.716106907924242	27.39121362417939	22.238336274462593
28-29	22.546564732697146	27.69939487327651	27.37195229611082	22.382088097915528
30-31	22.638622550671926	27.065848249060302	27.51759248506067	22.7779367152071
32-33	22.62158005206224	27.18339900124349	27.60455171619377	22.590469230500503
34-35	22.99240782651964	26.9951289612007	27.42539303967361	22.587070172606047
36-37	22.547028952589983	26.984310618216227	27.787667785155673	22.68099264403812
38-39	22.398583652994983	27.33403363824137	27.26085570964886	23.006526999114783
40-41	23.07115231357856	26.8637422902747	27.541376627398844	22.5237287687479
42-43	22.8636714499175	27.231010006349866	27.52315104418704	22.382167499545595
44-45	22.80820785941255	26.82540357039996	27.361294069321097	23.005094500866395
46-47	22.96422397147813	26.785189927080765	26.972657411787367	23.27792868965374
48-49	22.481793796152832	27.02317118882994	27.31152460020942	23.183510414807806
50-51	22.50799486742258	27.320777591144317	27.03105511477958	23.140172426653525
52-53	23.00784521445695	27.076753559805006	26.66272942460559	23.25267180113245
54-55	22.94534031650894	26.626708852751868	27.018922366461872	23.409028464277316
56-57	22.79143852851007	27.072504737436944	27.04101624277582	23.095040491277167
58-59	23.124241870514673	27.00798960468431	27.106153945672958	22.76161457912806
60-61	22.921594060354973	27.16531064477578	26.935217393973193	22.977877900896058
62-63	22.6414470623959	27.03773543027609	27.409531446349312	22.911286060978703
64-65	23.32324914095434	26.707900858455254	26.707759160201682	23.261090840388725
66-67	22.63193755521548	26.9526153782228	26.777247939999217	23.6381991265625
68-69	22.626442593216805	27.02826977567162	27.595818025770523	22.74946960534105
70-71	23.090413995809715	26.668064057134387	27.287872822360516	22.95364912469538
72-73	22.943027068775216	26.71409296612178	27.4362511412809	22.90662882382211
74-75	22.93891987381942	26.49268211160807	27.348347727399336	23.220050287173184
76-77	22.78218553757517	26.410585421629527	26.95009144409919	23.857137596696116
78-79	22.755953063919744	26.11609177003757	27.510424442798126	23.61753072324456
80-81	22.678851063930296	26.824270223414732	27.150452427234917	23.34642628542006
82-83	22.54920993451901	27.055414875894083	27.369145478514746	23.02622971107216
84-85	22.648500415337892	26.405431174568005	27.677937695065957	23.26813071502815
86-87	22.644854453502685	27.007470294078466	27.245457783438	23.10221746898085
88-89	22.81313784365044	26.51677508139369	27.019966129646196	23.650120945309673
90-91	22.475751026562694	26.569113705095848	27.217389580188023	23.73774568815343
92-93	22.590743681792343	26.45645001992292	27.60305623171766	23.349750066567086
94-95	22.846862055844632	26.298888602486787	27.410805416209914	23.44344392545866
96-97	22.83340745167909	26.81006914250267	27.45589014244885	22.90063326336939
98-99	22.259098099958596	26.868068757827928	27.504877923272264	23.367955218941212
100-101	22.854559916266293	26.354296359132963	27.186154465262412	23.60498925933833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	2.5
3	2.5
4	2.5
5	5.5
6	6.5
7	10.5
8	18.0
9	18.5
10	19.5
11	28.5
12	36.5
13	40.0
14	48.0
15	55.0
16	58.5
17	73.0
18	82.0
19	79.0
20	90.5
21	122.5
22	186.0
23	228.5
24	281.0
25	408.5
26	549.5
27	762.5
28	1162.5
29	1975.5
30	3663.5
31	6853.5
32	12284.5
33	20177.0
34	29285.5
35	37331.5
36	44877.0
37	49919.5
38	50777.0
39	48455.5
40	43639.5
41	38566.0
42	34132.5
43	31352.0
44	29925.5
45	30527.0
46	32737.5
47	32993.5
48	34009.0
49	37134.0
50	39580.0
51	38545.5
52	36804.0
53	37017.5
54	37868.5
55	37654.0
56	32075.5
57	26465.0
58	24376.0
59	22685.0
60	18720.5
61	13947.0
62	11066.5
63	8079.0
64	5090.0
65	3356.5
66	2700.5
67	2343.0
68	1793.5
69	1179.5
70	761.5
71	521.0
72	373.5
73	293.0
74	216.5
75	170.0
76	121.5
77	78.5
78	57.5
79	36.5
80	20.0
81	17.0
82	14.0
83	10.0
84	6.0
85	6.5
86	7.5
87	5.0
88	6.0
89	6.5
90	6.0
91	6.0
92	4.5
93	4.0
94	5.5
95	5.0
96	4.0
97	2.0
98	2.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1517301676774143
2	9.441827484593298E-5
3	2.832548245377989E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.039561257160445916
16-17	0.07699810313685836
18-19	0.027664554529858363
20-21	0.043101942467168405
22-23	0.06897254977495404
24-25	0.018694818419494733
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023415732161791377
38-39	0.005145795979103348
40-41	0.0061371878649856436
42-43	0.003729521856414352
44-45	0.012463212279663155
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016853662059999035
60-61	0.018742027556917694
62-63	0.031866167760502384
64-65	0.04999447653092151
66-67	0.018647609282071764
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017703426533612433
80-81	0.06226885226089279
82-83	0.053393534425375096
84-85	0.03214942258504018
86-87	0.0621272248486239
88-89	0.03531243479237894
90-91	0.0
92-93	0.0033046396196076545
94-95	0.0
96-97	0.0
98-99	8.025553361904302E-4
100-101	0.049380757744422944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1059117.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.11311325348646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3701185211723	37.50175177394405
2	14.632463788607694	14.958215576092893
3	5.209176980875917	7.987717589428963
4	2.3573131542760284	4.819584569137762
5	1.2717104190452306	3.2500539337148804
6	0.7826790031126256	2.4003096316332915
7	0.4928957495261763	1.7635405387385485
8	0.34021144858142127	1.3911413041179896
9	0.23915341441328608	1.1001488000277848
>10	1.125725156142264	10.034272847154146
>50	0.08975254269277476	3.2468862558846094
>100	0.08115109783984449	8.431077152967053
>500	0.006529398292785182	2.3351162304513
>1k	0.0011193254216203168	0.7801837967067882
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1829	0.17269102469321143	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1398	0.1319967482346143	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1358	0.128220017240777	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1280	0.12085539180279421	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1263	0.11925028113041335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	9.441827484593298E-5	0.0	0.0	9.441827484593298E-5
8	0.0	9.441827484593298E-5	0.0	0.0	9.441827484593298E-5
9	0.0	9.441827484593298E-5	0.0	0.0	9.441827484593298E-5
10-11	0.0	9.441827484593298E-5	0.0	0.0	9.441827484593298E-5
12-13	0.0	9.441827484593298E-5	0.0	0.0	2.832548245377989E-4
14-15	0.0	9.441827484593298E-5	0.0	0.0	4.248822368066984E-4
16-17	0.0	9.441827484593298E-5	0.0	9.441827484593298E-5	5.665096490755978E-4
18-19	0.0	9.441827484593298E-5	0.0	9.441827484593298E-5	5.665096490755978E-4
20-21	0.0	9.441827484593298E-5	0.0	1.4162741226889946E-4	6.609279239215308E-4
22-23	0.0	9.441827484593298E-5	0.0	2.3604568711483242E-4	7.553461987674638E-4
24-25	0.0	9.441827484593298E-5	0.0	3.776730993837319E-4	7.553461987674638E-4
26-27	0.0	9.441827484593298E-5	0.0	8.969736110363633E-4	8.497644736133968E-4
28-29	0.0	9.441827484593298E-5	0.0	0.001652319809803827	9.441827484593298E-4
30-31	0.0	9.441827484593298E-5	0.0	0.0049569594294114816	9.441827484593298E-4
32-33	0.0	9.441827484593298E-5	0.0	0.011849493493164588	0.0010386010233052628
34-35	0.0	9.441827484593298E-5	0.0	0.021857830626833483	0.0011330192981511957
36-37	0.0	9.441827484593298E-5	0.0	0.03923079319848516	0.0011330192981511957
38-39	0.0	9.441827484593298E-5	0.0	0.07397671834178848	0.0011330192981511957
40-41	0.0	9.441827484593298E-5	0.0	0.11264100189119805	0.0011330192981511957
42-43	0.0	9.441827484593298E-5	0.0	0.15187179508968318	0.0011330192981511957
44-45	0.0	9.441827484593298E-5	0.0	0.19469048273231382	0.0012274375729971285
46-47	0.0	9.441827484593298E-5	0.0	0.24888657249387935	0.0013218558478430616
48-49	0.0	9.441827484593298E-5	0.0	0.3067649749744362	0.0013218558478430616
50-51	0.0	9.441827484593298E-5	0.0	0.37191358461813	0.0013218558478430616
52-53	0.0	9.441827484593298E-5	0.0	0.4407917161182381	0.0014162741226889947
54-55	0.0	1.8883654969186595E-4	0.0	0.514154715673528	0.0014162741226889947
56-57	0.0	1.8883654969186595E-4	0.0	0.6032383579906658	0.0014162741226889947
58-59	0.0	1.8883654969186595E-4	0.0	0.7018110369298198	0.0014162741226889947
60-61	0.0	1.8883654969186595E-4	0.0	0.8146880845081328	0.0015106923975349276
62-63	0.0	1.8883654969186595E-4	0.0	0.9413974093513748	0.0015106923975349276
64-65	0.0	1.8883654969186595E-4	0.0	1.0632441930400514	0.0016051106723808607
66-67	0.0	2.832548245377989E-4	0.0	1.199064881405926	0.001652319809803827
68-69	0.0	2.832548245377989E-4	0.0	1.3605201313924713	0.0016995289472267936
70-71	0.0	2.832548245377989E-4	0.0	1.5333055743605286	0.0016995289472267936
72-73	0.0	2.832548245377989E-4	0.0	1.7188846935702098	0.0018883654969186596
74-75	0.0	2.832548245377989E-4	0.0	1.9075796158498068	0.002124411184033492
76-77	0.0	2.832548245377989E-4	0.0	2.1103900702188714	0.0021716203214564586
78-79	0.0	2.832548245377989E-4	0.0	2.343603209088325	0.0021716203214564586
80-81	0.0	2.832548245377989E-4	0.0	2.612978547223772	0.0021716203214564586
82-83	0.0	2.832548245377989E-4	0.0	2.9012375403284056	0.0022660385963023913
84-85	0.0	2.832548245377989E-4	0.0	3.1849172471030114	0.0022660385963023913
86-87	0.0	2.832548245377989E-4	0.0	3.4898410657179517	0.002407666008571291
88-89	0.0	2.832548245377989E-4	0.0	3.8312575475608455	0.0025492934208401906
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	45.014843	1
GTATCAA	1560	0.0	42.06074	1
AAACCGT	205	0.0	37.053734	6
ATCAACG	1710	0.0	36.64744	3
TCAACGC	1850	0.0	33.874123	4
CCGTGCG	185	0.0	33.387676	9
CGATTCG	30	0.003950879	31.668005	54-55
AACCGTG	240	0.0	31.650066	7
CCCATAA	945	0.0	30.6453	2
TATCAAC	2145	0.0	30.10076	2
CGCAGAG	2125	0.0	29.495985	8
GCCCATA	955	0.0	29.37454	1
CATAATA	970	0.0	28.876606	4
ACGCAGA	2195	0.0	28.549942	7
ACCGTGC	250	0.0	28.490438	8
CCATAAT	1045	0.0	28.167044	3
GACGTGC	95	0.0012288921	24.991613	8
GCAGAGT	2505	0.0	24.46789	9
GTACATG	3530	0.0	24.110197	1
ATAATAA	1275	0.0	23.830639	5
>>END_MODULE