FastQCFastQC Report
Fri 10 Feb 2017
SRR3224481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059117
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT14600.13785068127506214No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG12560.11858935320649183No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG12260.11575680496111385No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG12110.11434053083842484No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC11150.10527637645321528No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT11130.1050875399035234No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG10710.10112197235999422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.047.4013671
GGTATCA7450.046.110131
CCGTGCG1800.042.209729
ATCAACG20350.035.4686053
AACCGTG2150.035.3383677
AAACCGT2150.035.3383676
ACCGTGC2300.035.0982978
TAGACGC550.002583284634.535225
TCAACGC21700.033.2620354
TATCAAC24350.030.2271862
ACGCAGA25600.028.3802647
CGCAGAG25850.027.9220968
TATCGCG350.00840333927.136116-17
CTAACGC350.00840527527.13481988-89
CAACGCA26750.026.982665
AACGCAG28050.025.7321286
GTACATG40600.025.5008011
TGCGCGG1500.025.32582912-13
TACATGG41800.024.0837732
GCAGAGT30300.023.6646089
Produced by FastQC (version 0.11.5)