##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059117
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18775546044488	34.0	33.0	34.0	31.0	34.0
2	33.32316448513242	34.0	34.0	34.0	31.0	34.0
3	33.437099017389016	34.0	34.0	34.0	31.0	34.0
4	36.637520689404475	37.0	37.0	37.0	35.0	37.0
5	36.67064734113417	37.0	37.0	37.0	35.0	37.0
6	36.70854494829183	37.0	37.0	37.0	36.0	37.0
7	36.69153360771284	37.0	37.0	37.0	36.0	37.0
8	36.67588661120537	37.0	37.0	37.0	36.0	37.0
9	38.522042418354154	39.0	39.0	39.0	37.0	39.0
10-11	38.553877428083965	39.0	39.0	39.0	37.5	39.0
12-13	38.55041180530574	39.0	39.0	39.0	37.5	39.0
14-15	40.17499483059945	41.0	40.0	41.0	38.0	41.0
16-17	40.18619000544793	41.0	40.0	41.0	38.0	41.0
18-19	40.178753622121064	41.0	40.0	41.0	38.5	41.0
20-21	40.1809530958336	41.0	40.0	41.0	39.0	41.0
22-23	40.11403131098831	41.0	40.0	41.0	38.0	41.0
24-25	40.08132529267304	41.0	40.0	41.0	38.0	41.0
26-27	40.02399829291759	41.0	40.0	41.0	38.0	41.0
28-29	39.996746818340185	41.0	40.0	41.0	38.0	41.0
30-31	39.94276458597115	41.0	40.0	41.0	38.0	41.0
32-33	39.90568275270815	41.0	40.0	41.0	38.0	41.0
34-35	39.83348393048171	41.0	40.0	41.0	38.0	41.0
36-37	39.73693935608625	41.0	40.0	41.0	37.0	41.0
38-39	39.646109919867214	41.0	40.0	41.0	37.0	41.0
40-41	39.526119399462004	41.0	40.0	41.0	37.0	41.0
42-43	39.43567188516472	41.0	40.0	41.0	36.0	41.0
44-45	39.23142863347486	41.0	39.0	41.0	35.0	41.0
46-47	39.042553844381686	41.0	39.0	41.0	35.0	41.0
48-49	38.96658395625791	41.0	39.0	41.0	35.0	41.0
50-51	38.832527945448895	40.5	38.5	41.0	35.0	41.0
52-53	38.62167871915945	40.0	38.0	41.0	35.0	41.0
54-55	38.456378757021184	40.0	37.0	41.0	35.0	41.0
56-57	38.2601945771808	40.0	37.0	41.0	35.0	41.0
58-59	38.02392134202359	40.0	36.0	41.0	34.5	41.0
60-61	37.8099586731211	40.0	35.5	41.0	34.0	41.0
62-63	37.62085869644241	39.0	35.0	41.0	34.0	41.0
64-65	37.37697818088087	39.0	35.0	41.0	34.0	41.0
66-67	37.122195187122855	39.0	35.0	41.0	33.5	41.0
68-69	36.87330814253761	37.5	35.0	41.0	33.0	41.0
70-71	36.59621977552999	37.0	35.0	40.0	33.0	41.0
72-73	36.29140123329151	37.0	35.0	39.5	33.0	41.0
74-75	35.9713360280309	36.0	35.0	39.0	33.0	41.0
76-77	34.07697827529914	34.5	33.0	37.0	29.5	39.0
78-79	35.334333694955326	35.0	35.0	37.5	32.5	39.0
80-81	35.2043815744625	35.0	35.0	37.0	33.0	39.0
82-83	34.93976774992753	35.0	35.0	37.0	33.0	39.0
84-85	34.681616384214394	35.0	35.0	36.0	33.0	37.5
86-87	34.43897274805333	35.0	35.0	36.0	33.0	37.0
88-89	34.25224267007328	35.0	35.0	36.0	32.5	37.0
90-91	34.088546402333264	35.0	35.0	35.0	32.0	36.0
92-93	33.97125860504552	35.0	35.0	35.0	32.0	36.0
94-95	33.85547016996234	35.0	35.0	35.0	32.0	36.0
96-97	33.794093570398736	35.0	34.5	35.0	32.0	36.0
98-99	33.74694863740267	35.0	34.0	35.0	32.0	35.5
100-101	32.96731428161384	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	16.0
15	62.0
16	145.0
17	325.0
18	562.0
19	928.0
20	1246.0
21	1608.0
22	2019.0
23	2026.0
24	1954.0
25	2433.0
26	3372.0
27	4384.0
28	5876.0
29	6799.0
30	7331.0
31	8141.0
32	9589.0
33	13105.0
34	21571.0
35	42597.0
36	115588.0
37	250872.0
38	379893.0
39	176634.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19089871793291	21.364054128138417	14.48118348156572	22.96386367236295
2	14.778442797160277	21.58637808665143	40.30432898348341	23.330850132704885
3	17.932390850113823	28.51271389279938	30.35887442086191	23.196020836224893
4	12.374081428208592	18.722955065398818	41.34302442506352	27.55993908132907
5	12.94805012099702	38.73084843317594	35.852601742772514	12.468499703054526
6	27.028175357396776	39.45343149057186	20.63586931377742	12.882523838253942
7	24.788573878051245	32.727168008822446	24.831723029655837	17.652535083470475
8	24.39721012881485	35.307052950712716	22.1801746171575	18.11556230331493
9	25.699521393764808	16.68370916527636	22.116442281636495	35.50032715932234
10-11	24.426527003154515	26.300163249197205	29.447549232048964	19.825760515599317
12-13	25.19957662845559	24.105363241266073	29.167032537481695	21.528027592796644
14-15	22.488167029704933	26.091073979550888	26.47913308916767	24.941625901576504
16-17	22.179560898371	28.55378584235736	27.28400167309183	21.98265158617981
18-19	22.07829729859874	28.041236237356216	28.22417164487021	21.65629481917484
20-21	23.289264719489832	26.798592397739224	27.793063206138008	22.119079676632932
22-23	23.331553531207675	27.220633618998658	27.21189984198945	22.23591300780422
24-25	22.863061548114015	27.062607489558495	27.386179681233706	22.68815128109378
26-27	22.66960314987395	27.887384452690515	27.450547167851646	21.99246522958389
28-29	22.482078582300662	27.719797029085953	27.773806781056553	22.024317607556835
30-31	22.667218072515535	27.013315884737292	27.83046347361136	22.489002569135813
32-33	22.552167350964083	27.309013983838398	27.924952472133587	22.21386619306393
34-35	23.14591305127836	27.20262304988563	27.313002650904444	22.338461247931562
36-37	22.463455776095312	27.156242579149055	27.788670216557836	22.591631428197793
38-39	22.3838820451376	27.364351624985716	27.39810615824314	22.853660171633543
40-41	22.78445157617147	27.170274861039907	27.695948606244635	22.349324956543988
42-43	22.650330416752823	27.306992523016817	27.78404085667589	22.25863620355447
44-45	22.850113569997692	26.890193321583684	27.58181123726852	22.677881871150106
46-47	22.860204263859245	26.78338752212197	27.163701165129822	23.192707048888963
48-49	22.490744578697754	26.821790769410892	27.578322911050705	23.10914174084065
50-51	22.788181098027884	26.93134941654227	27.288203286322478	22.99226619910737
52-53	23.009708096185875	27.09712628267347	27.015973698820527	22.877191922320122
54-55	23.02045005414888	26.67958308666559	27.19501244904954	23.104954410135992
56-57	22.999838531028953	26.880004003675072	27.055400853802595	23.06475661149338
58-59	23.268353850477133	26.79821568150529	27.203253146053886	22.730177321963694
60-61	22.882434889376874	27.25442138320229	27.077660503884204	22.785483223536634
62-63	22.69229933992184	26.90477067217314	27.46939195575182	22.9335380321532
64-65	23.02328260239426	26.96793649804507	26.96647301478496	23.042307884775713
66-67	22.766889777050128	26.95537886749056	26.981815984447422	23.29591537101189
68-69	22.943829624111405	26.948297496877117	27.414912611165715	22.692960267845763
70-71	23.272263593162982	26.724667812904524	27.250672022071214	22.752396571861276
72-73	22.929147582372863	26.656261772778645	27.438706016426888	22.9758846284216
74-75	23.01629565005566	26.42720303800241	27.279469595899226	23.277031716042703
76-77	22.783771522126763	26.317953647083147	27.187596041406326	23.710678789383763
78-79	22.799740893257276	26.25434008515396	27.245725541371623	23.700193480217145
80-81	22.460681520314548	26.828535924852982	27.32837351422604	23.382409040606433
82-83	22.50172433246735	26.922341541989915	27.41761798045615	23.158316145086584
84-85	22.66900451943954	26.455174080804163	27.505602679277473	23.370218720478825
86-87	22.455356679195972	26.987622708350443	27.288203286322478	23.268817326131106
88-89	22.816695417031358	26.413842852111713	26.939941479553255	23.82952025130368
90-91	22.660527590436182	26.685200974018926	26.96987207267941	23.684399362865484
92-93	22.784640412721163	26.61877771766481	27.210072163887467	23.386509705726564
94-95	22.69801164555002	26.44495367367345	27.098375344744724	23.758659336031805
96-97	22.62096633327574	26.948627960839076	27.190197117032394	23.24020858885279
98-99	21.78659203846223	27.032471388902263	27.51853666780913	23.66239990482638
100-101	22.464676317483953	26.691755075277037	27.089292657105297	23.754275950133717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	3.0
3	3.0
4	1.5
5	2.5
6	3.0
7	9.5
8	16.0
9	15.0
10	18.0
11	25.0
12	24.5
13	28.0
14	38.5
15	44.0
16	53.5
17	67.0
18	76.0
19	98.5
20	124.5
21	141.0
22	173.0
23	223.0
24	300.5
25	419.0
26	559.0
27	828.0
28	1259.5
29	2170.0
30	4053.5
31	7413.5
32	13205.5
33	21334.0
34	30345.5
35	38335.0
36	45667.0
37	50281.5
38	50286.0
39	47325.5
40	42691.5
41	37931.0
42	33969.0
43	31612.5
44	30560.5
45	31104.5
46	32241.5
47	33097.0
48	34208.0
49	37129.5
50	40690.0
51	39463.0
52	36269.5
53	36271.0
54	37605.5
55	36697.5
56	31442.0
57	26102.5
58	24129.5
59	23166.0
60	19152.5
61	13882.5
62	10736.5
63	7620.5
64	4635.0
65	2914.0
66	2343.5
67	2034.5
68	1475.0
69	942.0
70	598.0
71	403.0
72	302.5
73	224.0
74	164.0
75	123.0
76	92.5
77	55.0
78	32.0
79	14.5
80	8.0
81	5.0
82	2.5
83	1.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.45358539235986206
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0010386010233052628
22-23	9.913918858822962E-4
24-25	2.3604568711483245E-4
26-27	0.00254929342084019
28-29	0.004626495467450717
30-31	5.665096490755978E-4
32-33	1.4162741226889946E-4
34-35	0.004201613230644018
36-37	0.002218829458879425
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0074118345754057395
46-47	0.07364625437982772
48-49	7.553461987674638E-4
50-51	0.0
52-53	9.441827484593298E-5
54-55	0.0
56-57	0.008497644736133968
58-59	0.04244101454324688
60-61	0.03229104999730908
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.304639619607654E-4
78-79	0.0088753178355177
80-81	0.0068925340637531075
82-83	0.0010858101607282293
84-85	0.002407666008571291
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06859487667557031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1059117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.7602721559468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36238963093093	36.00772201889786
2	15.154540017035725	15.08188071291769
3	5.384927526284202	8.038663777438536
4	2.4661779880924652	4.908707514899456
5	1.3205740343466765	3.2856061675583637
6	0.8148204061296661	2.4327411100338776
7	0.5296247265959845	1.8447989375154572
8	0.35596877276416244	1.4170482409410485
9	0.23953427967020963	1.0727361852361474
>10	1.1789500642267698	10.242646230974788
>50	0.09559131336796298	3.335377531024078
>100	0.08892113882947203	9.091706031770556
>500	0.006080077505395622	2.149213359591669
>1k	0.0019000242204361317	1.091152181200398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1460	0.13785068127506214	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1256	0.11858935320649183	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1226	0.11575680496111385	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1211	0.11434053083842484	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1115	0.10527637645321528	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1113	0.1050875399035234	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1071	0.10112197235999422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.441827484593298E-5
7	0.0	0.0	0.0	0.0	9.441827484593298E-5
8	0.0	0.0	0.0	0.0	9.441827484593298E-5
9	0.0	0.0	0.0	0.0	9.441827484593298E-5
10-11	0.0	0.0	0.0	0.0	9.441827484593298E-5
12-13	0.0	0.0	0.0	9.441827484593298E-5	9.441827484593298E-5
14-15	0.0	0.0	0.0	9.441827484593298E-5	9.441827484593298E-5
16-17	0.0	0.0	0.0	1.8883654969186595E-4	1.8883654969186595E-4
18-19	0.0	0.0	0.0	2.832548245377989E-4	1.8883654969186595E-4
20-21	0.0	0.0	0.0	5.193005116526314E-4	2.3604568711483242E-4
22-23	0.0	0.0	0.0	6.137187864985643E-4	2.832548245377989E-4
24-25	0.0	0.0	0.0	7.553461987674638E-4	2.832548245377989E-4
26-27	0.0	0.0	0.0	0.0012274375729971285	4.248822368066984E-4
28-29	0.0	0.0	0.0	0.0018411563594956932	4.720913742296649E-4
30-31	0.0	0.0	0.0	0.005145795979103347	4.720913742296649E-4
32-33	0.0	0.0	0.0	0.012085539180279423	4.720913742296649E-4
34-35	0.0	0.0	0.0	0.022235503726217215	7.553461987674638E-4
36-37	0.0	0.0	0.0	0.039608466297868884	7.553461987674638E-4
38-39	0.0	0.0	0.0	0.07477927367797892	7.553461987674638E-4
40-41	0.0	0.0	0.0	0.11377402118934923	7.553461987674638E-4
42-43	0.0	0.0	0.0	0.1533824874872181	7.553461987674638E-4
44-45	0.0	0.0	0.0	0.19605954771757983	7.553461987674638E-4
46-47	0.0	0.0	0.0	0.2505861014411061	7.553461987674638E-4
48-49	0.0	0.0	0.0	0.30917264098300756	7.553461987674638E-4
50-51	0.0	0.0	0.0	0.374746132863508	7.553461987674638E-4
52-53	0.0	0.0	0.0	0.4441907740126917	7.553461987674638E-4
54-55	0.0	0.0	0.0	0.5189700476906707	8.025553361904302E-4
56-57	0.0	0.0	0.0	0.6084313631071921	8.497644736133968E-4
58-59	0.0	9.441827484593298E-5	0.0	0.7077121791076906	8.497644736133968E-4
60-61	0.0	9.441827484593298E-5	0.0	0.821344572884771	9.441827484593298E-4
62-63	0.0	9.441827484593298E-5	0.0	0.9488092439267806	0.0011330192981511957
64-65	0.0	9.441827484593298E-5	0.0	1.072166720012992	0.0011330192981511957
66-67	0.0	9.441827484593298E-5	0.0	1.208789963715057	0.0011330192981511957
68-69	0.0	9.441827484593298E-5	0.0	1.3718503243739832	0.0011330192981511957
70-71	0.0	9.441827484593298E-5	0.0	1.5468073876634971	0.0011802284355741622
72-73	0.0	9.441827484593298E-5	0.0	1.7342748723700971	0.0012274375729971288
74-75	0.0	9.441827484593298E-5	0.0	1.9262744342693017	0.0013218558478430616
76-77	0.0	9.441827484593298E-5	0.0	2.1310204632727077	0.0013218558478430616
78-79	0.0	9.441827484593298E-5	0.0	2.3671605686623858	0.0013218558478430616
80-81	0.0	9.441827484593298E-5	0.0	2.639085200218673	0.0013690649852660282
82-83	0.0	9.441827484593298E-5	0.0	2.9296574410570315	0.0015106923975349276
84-85	0.0	9.441827484593298E-5	0.0	3.215225513328556	0.0015106923975349276
86-87	0.0	9.441827484593298E-5	0.0	3.5245869908612555	0.0016051106723808605
88-89	0.0	9.441827484593298E-5	0.0	3.8690248574992188	0.0016995289472267936
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1540	0.0	47.401367	1
GGTATCA	745	0.0	46.11013	1
CCGTGCG	180	0.0	42.20972	9
ATCAACG	2035	0.0	35.468605	3
AACCGTG	215	0.0	35.338367	7
AAACCGT	215	0.0	35.338367	6
ACCGTGC	230	0.0	35.098297	8
TAGACGC	55	0.0025832846	34.53522	5
TCAACGC	2170	0.0	33.262035	4
TATCAAC	2435	0.0	30.227186	2
ACGCAGA	2560	0.0	28.380264	7
CGCAGAG	2585	0.0	27.922096	8
TATCGCG	35	0.008403339	27.1361	16-17
CTAACGC	35	0.008405275	27.134819	88-89
CAACGCA	2675	0.0	26.98266	5
AACGCAG	2805	0.0	25.732128	6
GTACATG	4060	0.0	25.500801	1
TGCGCGG	150	0.0	25.325829	12-13
TACATGG	4180	0.0	24.083773	2
GCAGAGT	3030	0.0	23.664608	9
>>END_MODULE