FastQCFastQC Report
Fri 10 Feb 2017
SRR3224480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118808
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC17310.15471823583671193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1100.051.833849
ATCCCGT651.331044E-436.5256
AAACCGT1850.035.9327056
GTATCAA15450.035.7061421
ACCGTGC2150.035.335828
ATCAACG15100.034.9043583
CTATCCG550.002575776334.5558939
TCAACGC15450.034.1136444
GGTATCA8900.033.6638681
AACCGTG2150.033.127337
CGCAGAG17950.029.362448
ACGCAGA18700.028.438727
CAACGCA18550.028.412715
AACGCAG19250.027.6261846
TATCAAC19800.027.338412
GTATTAC3500.024.4578291
TAATACT13800.024.0853274
GCAGAGT22850.023.2893129
TGCGCGG1354.0017767E-1122.8803512-13
ACTTAAT11850.021.6714951
Produced by FastQC (version 0.11.5)