##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224480_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118808
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40400944576728	34.0	31.0	34.0	31.0	34.0
2	32.573285139183845	34.0	31.0	34.0	31.0	34.0
3	32.71108358181207	34.0	33.0	34.0	31.0	34.0
4	35.990988623606555	37.0	37.0	37.0	35.0	37.0
5	36.02396657871592	37.0	37.0	37.0	35.0	37.0
6	36.122862010282375	37.0	37.0	37.0	35.0	37.0
7	36.081928266512215	37.0	37.0	37.0	35.0	37.0
8	36.1132374813194	37.0	37.0	37.0	35.0	37.0
9	37.8615490772322	39.0	39.0	39.0	37.0	39.0
10-11	37.94432780244689	39.0	39.0	39.0	37.0	39.0
12-13	37.971137138812026	39.0	39.0	39.0	37.0	39.0
14-15	39.51710838678308	41.0	40.0	41.0	38.0	41.0
16-17	39.36479449557029	41.0	40.0	41.0	37.5	41.0
18-19	39.473923586531384	41.0	40.0	41.0	37.5	41.0
20-21	39.55232086291839	41.0	40.0	41.0	38.0	41.0
22-23	39.51883611844034	41.0	40.0	41.0	38.0	41.0
24-25	39.48890158096832	41.0	40.0	41.0	37.0	41.0
26-27	39.36692980386268	41.0	40.0	41.0	37.0	41.0
28-29	39.30029325853945	41.0	40.0	41.0	37.0	41.0
30-31	39.22938967186506	41.0	40.0	41.0	37.0	41.0
32-33	39.0540351874495	41.0	40.0	41.0	36.0	41.0
34-35	39.07787842060479	41.0	39.0	41.0	36.0	41.0
36-37	39.10035323308378	41.0	40.0	41.0	36.0	41.0
38-39	39.02403763648454	41.0	39.0	41.0	36.0	41.0
40-41	38.98008505480833	41.0	39.0	41.0	35.0	41.0
42-43	38.88226934380162	41.0	39.0	41.0	35.0	41.0
44-45	38.774777262944134	41.0	39.0	41.0	35.0	41.0
46-47	38.67584250380763	41.0	39.0	41.0	35.0	41.0
48-49	38.55103601332847	40.5	39.0	41.0	35.0	41.0
50-51	37.38644298217388	39.0	37.5	40.0	33.0	40.5
52-53	37.761603867687754	39.5	38.0	40.5	33.5	41.0
54-55	38.33574885056238	40.0	38.0	41.0	34.0	41.0
56-57	38.29401470136074	40.0	38.0	41.0	34.0	41.0
58-59	38.23667912635591	40.0	38.0	41.0	34.0	41.0
60-61	38.08405418981631	40.0	37.5	41.0	34.0	41.0
62-63	37.86932923254035	40.0	37.0	41.0	33.5	41.0
64-65	37.707849336079114	40.0	37.0	41.0	33.0	41.0
66-67	37.52248330365889	40.0	36.0	41.0	33.0	41.0
68-69	37.278296633560004	39.0	36.0	41.0	33.0	41.0
70-71	37.0212954322815	39.0	35.0	41.0	32.5	41.0
72-73	36.73210372110317	39.0	35.0	41.0	32.0	41.0
74-75	36.42928143166656	38.0	35.0	40.0	32.0	41.0
76-77	36.03336765557629	37.0	35.0	39.5	31.0	41.0
78-79	35.70480279011234	37.0	35.0	39.0	31.0	41.0
80-81	35.28785412689219	36.0	35.0	39.0	31.0	39.5
82-83	34.90478884670113	36.0	35.0	37.5	31.0	39.0
84-85	34.545384015845435	35.5	35.0	37.0	31.0	39.0
86-87	34.196693266404964	35.0	34.5	37.0	30.5	38.0
88-89	33.9209663320248	35.0	34.0	36.0	30.0	37.0
90-91	33.67174037010818	35.0	34.0	36.0	30.0	37.0
92-93	33.45464547983211	35.0	34.0	36.0	29.5	36.5
94-95	33.28153311381399	35.0	34.0	35.0	29.0	36.0
96-97	33.12928268299834	35.0	34.0	35.0	29.0	36.0
98-99	33.033703727538594	35.0	34.0	35.0	29.0	36.0
100-101	32.0194698286033	34.5	32.5	35.0	26.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	124.0
8	438.0
9	361.0
10	387.0
11	756.0
12	1228.0
13	1462.0
14	1570.0
15	1719.0
16	1732.0
17	1953.0
18	2183.0
19	2730.0
20	3235.0
21	3873.0
22	4389.0
23	4276.0
24	4053.0
25	4295.0
26	4543.0
27	5050.0
28	5688.0
29	6495.0
30	7882.0
31	9675.0
32	12612.0
33	17608.0
34	26391.0
35	44443.0
36	87055.0
37	182585.0
38	418424.0
39	249526.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.976998261486095	23.975019829084925	15.634002538839045	21.41397937058993
2	12.890594275335893	22.830995130531782	43.83576091697592	20.442649677156403
3	16.16544780775219	30.15920543865514	32.215057838445624	21.46028891514704
4	10.5817977704843	19.808045705786874	44.67227620825021	24.937880315478616
5	11.189766251224517	40.29699465860094	37.65471823583671	10.858520854337831
6	24.17349536292197	43.18015244796247	21.402153005698924	11.244199183416637
7	22.203631007286326	34.42091940708325	27.529120278010165	15.846329307620252
8	21.461591264989167	39.51607425045226	23.56597378638694	15.456360698171626
9	23.52217717427834	18.102927401305674	24.25760273433869	34.11729269007729
10-11	21.51852686073035	28.843510235893916	32.02703234156352	17.610930561812214
12-13	22.216904062180465	26.383391967165053	32.084638293612485	19.315065677041996
14-15	19.817423742513736	28.5546441198088	28.944180074428953	22.683752063248512
16-17	19.50911349899862	31.651351527831572	29.35318621378103	19.48634875938878
18-19	19.601524345950537	30.793576289938645	30.679809121489477	18.92509024262134
20-21	20.64234436872321	29.61586935829496	30.459562760175313	19.282223512806514
22-23	20.347264706829378	29.89807271933685	30.001914043353977	19.7527485304798
24-25	20.00761656836049	29.981655870568403	29.90866375711371	20.1020638039574
26-27	19.95427276172498	30.043850240613224	30.077055223058828	19.92482177460297
28-29	19.93536871384545	29.891724049166612	30.27918999506618	19.89371724192176
30-31	20.221029881802778	29.601415077475313	30.163799329286167	20.013755711435742
32-33	20.091070138933578	29.616386368349172	30.259570900458343	20.032972592258904
34-35	20.11596270316265	29.88171339497036	30.037012606273823	19.965311295593168
36-37	20.317513389899755	29.2113480909056	30.232297215991533	20.238841303203117
38-39	20.084907793836475	29.844209081084756	30.031428129606176	20.039454995472596
40-41	20.296306426736137	29.31207013601456	30.390783206650696	20.00084023059861
42-43	20.346067865663066	29.53080573380206	30.10791325108222	20.015213149452656
44-45	20.510969654332754	29.56599626165551	29.729180093986017	20.19385399002572
46-47	20.479519274084563	29.204608833687278	29.743977518930866	20.571894373297294
48-49	20.20851656405746	29.416754259890883	29.88265189380126	20.492077282250396
50-51	20.163334548912772	29.619738149888097	29.46519867573346	20.751728625465674
52-53	20.319706330308684	29.538982560010297	29.61415184732322	20.527159262357795
54-55	20.283685851370386	29.49245983225004	29.565349908116495	20.658504408263077
56-57	20.478053641935723	29.672307345097348	29.36514994760046	20.484489065366475
58-59	20.39383083287332	29.362678818462808	29.553224822382784	20.69026552628109
60-61	20.7390766213257	29.515326544779025	29.454938478819702	20.290658355075568
62-63	20.338817117345467	29.59683212790221	29.589232525048516	20.475118229703806
64-65	20.526935634015544	29.427190222100606	29.36933335837185	20.676540785512
66-67	20.35205472532964	29.520487357238416	29.253100626718382	20.87435729071356
68-69	20.399434040514546	29.40607324938685	29.700046835560705	20.4944458745379
70-71	20.496099420097103	29.085955767209388	29.740804499074013	20.677140313619493
72-73	20.439163824355923	29.256315650227744	29.63225146763341	20.672269057782927
74-75	20.291730127063804	29.161125054522312	29.56208750741861	20.985057310995273
76-77	20.27707166913358	29.29327462799694	29.54514983804192	20.88450386482757
78-79	20.328021412173612	29.310281633733116	29.306169409341543	21.05552754475173
80-81	20.3524330328496	29.20296760293755	29.49930755633343	20.945291807879414
82-83	20.306342778216717	29.452448949204747	29.45204652554116	20.78916174703738
84-85	20.452662810507743	29.170863245834376	29.628228907244313	20.748245036413568
86-87	19.734681539144	29.55158085657456	29.880076341885726	20.83366126239572
88-89	20.330680633983373	28.907463695452652	29.614934633261818	21.14692103730216
90-91	20.244134829211088	29.07670485016196	29.6082080213942	21.070952299232754
92-93	20.35947545918773	29.004736455872482	29.642669755776215	20.993118329163572
94-95	20.35107900551301	28.781256480110972	29.598376128880023	21.269288385495994
96-97	20.409936289336507	28.99804971004855	29.718995573860752	20.87301842675419
98-99	20.10805322307239	29.11157976779596	29.6037586683238	21.176608340807856
100-101	20.244241483673342	28.9469483675131	29.5015955327765	21.307214616037058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	1.5
3	5.0
4	5.0
5	7.0
6	15.5
7	23.0
8	32.0
9	43.0
10	40.5
11	49.0
12	75.0
13	92.0
14	97.5
15	108.5
16	132.5
17	153.0
18	190.0
19	221.0
20	243.5
21	298.0
22	359.0
23	430.5
24	558.0
25	739.5
26	1139.5
27	1998.0
28	3901.5
29	8170.0
30	15825.5
31	27751.5
32	42921.5
33	57500.0
34	69601.0
35	78213.5
36	83299.5
37	83598.5
38	77029.0
39	67112.5
40	55254.5
41	42897.5
42	34052.0
43	27834.5
44	23398.0
45	21168.0
46	20436.5
47	19932.5
48	19788.0
49	20631.5
50	21402.5
51	20436.0
52	19221.0
53	19433.0
54	19600.5
55	18954.5
56	16354.0
57	13695.0
58	12501.0
59	11366.5
60	9557.5
61	7375.0
62	5752.5
63	4229.0
64	2878.0
65	2057.0
66	1569.5
67	1281.0
68	1020.5
69	721.5
70	473.5
71	356.5
72	275.0
73	210.5
74	158.5
75	133.0
76	114.5
77	79.5
78	48.5
79	31.0
80	24.5
81	18.0
82	13.0
83	14.0
84	9.0
85	2.5
86	4.5
87	7.0
88	6.0
89	7.0
90	9.0
91	8.5
92	6.0
93	3.5
94	2.0
95	1.0
96	2.5
97	4.0
98	3.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15748904190888874
2	0.0
3	1.7876168207592365E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03807623828217174
16-17	0.07606309572330552
18-19	0.026412038526717724
20-21	0.04035544972863976
22-23	0.06757191582469914
24-25	0.017339883161364596
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0215407826901488
38-39	0.005809754667467519
40-41	0.006033206770062424
42-43	0.003485852800480511
44-45	0.011708890175973
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.469042051898091E-5
58-59	0.01613324180735211
60-61	0.018725286197453003
62-63	0.02936160628097046
64-65	0.047461226591157736
66-67	0.01818900115122523
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017161121479288673
80-81	0.059572330551801556
82-83	0.05215372074565072
84-85	0.030166033850312118
86-87	0.06077897190581405
88-89	0.03463507590221021
90-91	0.0
92-93	0.003485852800480511
94-95	0.0
96-97	0.0
98-99	6.703563077847137E-4
100-101	0.04732715532960079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1118808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.49747000159266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.42803393965376	30.63441293561239
2	13.22254416214432	11.502944360752133
3	5.312198619193401	6.932016002426006
4	2.9477193572383613	5.128733372583584
5	1.817023495300114	3.9517962489502874
6	1.2379294403100642	3.230807921638524
7	0.9113204099867536	2.7748092536666555
8	0.653999785817221	2.275786885170607
9	0.5292850753371587	2.0720305518091897
>10	2.7205699096319345	20.575061364631544
>50	0.14432113543333444	4.377845807890164
>100	0.07193605517678754	5.526366399608288
>500	0.0027027994732474058	0.7695981776594035
>1k	4.1581530357652404E-4	0.24779071760126503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1731	0.15471823583671193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	8.938084103796184E-5	8.938084103796184E-5
14-15	0.0	0.0	0.0	8.938084103796184E-5	8.938084103796184E-5
16-17	0.0	0.0	0.0	8.938084103796184E-5	8.938084103796184E-5
18-19	0.0	0.0	0.0	1.3407126155694276E-4	8.938084103796184E-5
20-21	0.0	0.0	0.0	3.1283294363286643E-4	8.938084103796184E-5
22-23	0.0	0.0	0.0	4.4690420518980913E-4	8.938084103796184E-5
24-25	0.0	0.0	0.0	5.36285046227771E-4	8.938084103796184E-5
26-27	0.0	0.0	0.0	6.703563077847137E-4	2.681425231138855E-4
28-29	0.0	0.0	0.0	9.8318925141758E-4	2.681425231138855E-4
30-31	0.0	0.0	0.0	0.002502663549062931	2.681425231138855E-4
32-33	0.0	0.0	0.0	0.0065694918162901945	2.681425231138855E-4
34-35	0.0	0.0	0.0	0.012915531529985484	2.681425231138855E-4
36-37	0.0	0.0	0.0	0.022658043203123326	2.681425231138855E-4
38-39	0.0	0.0	0.0	0.04048952099019671	2.681425231138855E-4
40-41	0.0	0.0	0.0	0.06448827680888947	2.681425231138855E-4
42-43	0.0	0.0	0.0	0.09094500575612616	2.681425231138855E-4
44-45	0.0	0.0	0.0	0.12053006413969153	2.681425231138855E-4
46-47	0.0	0.0	0.0	0.15628240055487624	2.681425231138855E-4
48-49	0.0	0.0	0.0	0.19873830004790813	3.5752336415184735E-4
50-51	0.0	0.0	0.0	0.24946192733695144	3.5752336415184735E-4
52-53	0.0	0.0	0.0	0.30148157682104526	4.4690420518980913E-4
54-55	0.0	0.0	0.0	0.35680831742354363	4.4690420518980913E-4
56-57	0.0	0.0	0.0	0.4258550171253691	4.4690420518980913E-4
58-59	0.0	0.0	0.0	0.5049123710234463	4.4690420518980913E-4
60-61	0.0	0.0	0.0	0.5961255193026864	4.4690420518980913E-4
62-63	0.0	0.0	0.0	0.6927908988852421	4.4690420518980913E-4
64-65	8.938084103796184E-5	0.0	0.0	0.7926292983246455	4.4690420518980913E-4
66-67	8.938084103796184E-5	0.0	0.0	0.9033721603706802	4.4690420518980913E-4
68-69	8.938084103796184E-5	0.0	0.0	1.0357004955273827	4.4690420518980913E-4
70-71	8.938084103796184E-5	0.0	0.0	1.1857262372096016	4.4690420518980913E-4
72-73	8.938084103796184E-5	0.0	0.0	1.356264881910033	4.9159462570879E-4
74-75	8.938084103796184E-5	0.0	0.0	1.5337305417909062	5.36285046227771E-4
76-77	8.938084103796184E-5	0.0	0.0	1.7110621304102223	5.36285046227771E-4
78-79	8.938084103796184E-5	0.0	0.0	1.9144482341921045	5.36285046227771E-4
80-81	8.938084103796184E-5	0.0	0.0	2.1514415342042605	5.80975466746752E-4
82-83	8.938084103796184E-5	0.0	0.0	2.421505745400462	6.256658872657329E-4
84-85	8.938084103796184E-5	0.0	0.0	2.691391194914588	6.256658872657329E-4
86-87	8.938084103796184E-5	0.0	0.0	2.983487783426647	6.703563077847137E-4
88-89	8.938084103796184E-5	0.0	0.0	3.308789354384309	7.150467283036947E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	110	0.0	51.83384	9
ATCCCGT	65	1.331044E-4	36.525	6
AAACCGT	185	0.0	35.932705	6
GTATCAA	1545	0.0	35.706142	1
ACCGTGC	215	0.0	35.33582	8
ATCAACG	1510	0.0	34.904358	3
CTATCCG	55	0.0025757763	34.555893	9
TCAACGC	1545	0.0	34.113644	4
GGTATCA	890	0.0	33.663868	1
AACCGTG	215	0.0	33.12733	7
CGCAGAG	1795	0.0	29.36244	8
ACGCAGA	1870	0.0	28.43872	7
CAACGCA	1855	0.0	28.41271	5
AACGCAG	1925	0.0	27.626184	6
TATCAAC	1980	0.0	27.33841	2
GTATTAC	350	0.0	24.457829	1
TAATACT	1380	0.0	24.085327	4
GCAGAGT	2285	0.0	23.289312	9
TGCGCGG	135	4.0017767E-11	22.88035	12-13
ACTTAAT	1185	0.0	21.671495	1
>>END_MODULE