FastQCFastQC Report
Fri 10 Feb 2017
SRR3224480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118808
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC17130.1531093806980286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.041.2728231
CCGTGCG1400.040.7052239
GGTATCA9650.038.580071
TCAACGC18800.036.374884
ATCAACG18950.036.0869563
CAACGCA20350.033.3709535
AACGCAG20650.032.8861476
CGCAGAG22550.030.3258468
ACGCAGA22800.029.9933247
TATCAAC24050.029.0268042
AACCGTG2251.0186341E-1025.3276967
TAGACCG950.001228126624.9944365
GCAGAGT28000.024.0839279
AGTTGCG1000.001655761523.7447172
ACCGTGC2402.3101165E-1023.7447178
TAATACT12400.023.7447154
AAACCGT2452.9831426E-1023.260136
CAGTACG1050.002199027222.6140169
AGAGTAC26000.020.91361410-11
TGCGCGG1652.7284841E-1120.14703212-13
Produced by FastQC (version 0.11.5)