##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118808
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.201260627382	34.0	33.0	34.0	31.0	34.0
2	33.33853619209015	34.0	34.0	34.0	31.0	34.0
3	33.45395277831406	34.0	34.0	34.0	31.0	34.0
4	36.650966028129936	37.0	37.0	37.0	35.0	37.0
5	36.69036599666788	37.0	37.0	37.0	35.0	37.0
6	36.72861652759008	37.0	37.0	37.0	37.0	37.0
7	36.7057725722376	37.0	37.0	37.0	36.0	37.0
8	36.69316272318396	37.0	37.0	37.0	36.0	37.0
9	38.548099405796165	39.0	39.0	39.0	38.0	39.0
10-11	38.57866586581433	39.0	39.0	39.0	37.5	39.0
12-13	38.59087260727489	39.0	39.0	39.0	38.0	39.0
14-15	40.22925381298668	41.0	40.0	41.0	39.0	41.0
16-17	40.244019081021946	41.0	40.0	41.0	39.0	41.0
18-19	40.254851145147335	41.0	40.0	41.0	39.0	41.0
20-21	40.26968344881338	41.0	40.0	41.0	39.0	41.0
22-23	40.20375971569742	41.0	40.0	41.0	39.0	41.0
24-25	40.19021002710027	41.0	40.0	41.0	39.0	41.0
26-27	40.15262404273119	41.0	40.0	41.0	39.0	41.0
28-29	40.122646155551266	41.0	40.0	41.0	38.0	41.0
30-31	40.064955738607516	41.0	40.0	41.0	38.0	41.0
32-33	40.02217672737413	41.0	40.0	41.0	38.0	41.0
34-35	39.96046551329629	41.0	40.0	41.0	38.0	41.0
36-37	39.89142104811549	41.0	40.0	41.0	38.0	41.0
38-39	39.831879107049645	41.0	40.0	41.0	38.0	41.0
40-41	39.74840365817906	41.0	40.0	41.0	38.0	41.0
42-43	39.697017271953726	41.0	40.0	41.0	37.5	41.0
44-45	39.53821746001101	41.0	40.0	41.0	37.0	41.0
46-47	39.385581797770485	41.0	40.0	41.0	36.5	41.0
48-49	39.355291077646925	41.0	40.0	41.0	36.0	41.0
50-51	39.29751083295794	41.0	39.5	41.0	36.0	41.0
52-53	39.17750454054672	41.0	39.0	41.0	35.5	41.0
54-55	39.08375878613667	41.0	39.0	41.0	35.0	41.0
56-57	38.97286844570293	41.0	39.0	41.0	35.0	41.0
58-59	38.81969917984141	41.0	38.5	41.0	35.0	41.0
60-61	38.67075584014415	40.0	38.0	41.0	35.0	41.0
62-63	38.52924988022967	40.0	38.0	41.0	35.0	41.0
64-65	38.34287071597629	40.0	37.0	41.0	35.0	41.0
66-67	38.125102787967194	40.0	37.0	41.0	34.5	41.0
68-69	37.91762259476157	39.5	36.0	41.0	34.0	41.0
70-71	37.657074761710675	39.0	36.0	41.0	34.0	41.0
72-73	37.37524177517501	39.0	35.0	41.0	34.0	41.0
74-75	37.04477265089274	38.5	35.0	40.0	34.0	41.0
76-77	35.0349523778879	35.5	33.5	38.5	30.5	39.5
78-79	36.272815800387555	37.0	35.0	39.0	33.5	40.5
80-81	36.075255986728735	36.5	35.0	39.0	34.0	40.0
82-83	35.72057672093871	36.0	35.0	38.0	34.0	39.0
84-85	35.35967163266619	36.0	35.0	37.0	33.5	39.0
86-87	35.02150547725794	35.0	35.0	37.0	33.0	38.0
88-89	34.73588497758328	35.0	35.0	36.5	33.0	37.0
90-91	34.50125982295443	35.0	35.0	36.0	33.0	37.0
92-93	34.32554379303687	35.0	35.0	36.0	33.0	36.5
94-95	34.159135437000806	35.0	35.0	35.5	33.0	36.0
96-97	34.055650299247056	35.0	35.0	35.0	33.0	36.0
98-99	33.96234921452117	35.0	35.0	35.0	32.5	36.0
100-101	33.24235749118705	35.0	34.0	35.0	30.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	10.0
15	47.0
16	117.0
17	266.0
18	540.0
19	870.0
20	1363.0
21	1851.0
22	2663.0
23	2580.0
24	2181.0
25	2129.0
26	2468.0
27	3082.0
28	3919.0
29	4821.0
30	5767.0
31	6786.0
32	8764.0
33	11662.0
34	18085.0
35	32857.0
36	74089.0
37	176033.0
38	443459.0
39	312273.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.958882457802545	24.150191061638147	15.696959627845155	21.19396685271415
2	12.801928481026234	22.955323880415584	44.12383536764127	20.118912270916905
3	16.04341406210896	30.32379103474412	32.449088672944775	21.183706230202144
4	10.668139662926972	19.887952177674812	44.66584078769548	24.778067371702743
5	11.031562162587324	40.246852006778646	37.8501047543457	10.871481076288335
6	24.22471058483672	42.980654410765744	21.444877047715067	11.349757956682469
7	22.178246848431545	34.313483636155624	27.620020593345775	15.888248922067058
8	21.340748367905842	39.48872371309465	23.504837291116974	15.665690627882531
9	23.570085305074688	18.103016782146714	24.350737570700247	33.97616034207835
10-11	21.5497207742526	28.891507747531303	31.9709458638122	17.5878256144039
12-13	22.28250959950233	26.566622691292874	32.056349257423975	19.09451845178082
14-15	19.935994379732715	28.64070510757878	28.903306018548314	22.519994494140192
16-17	19.290620017018114	31.858728217889038	29.485085912864406	19.365565852228443
18-19	19.4570471430308	30.817441419796783	30.79992277495334	18.925588662219077
20-21	20.478084302043666	29.698198558542938	30.698155208466943	19.125561930946457
22-23	20.305714618982947	29.751914682712595	30.238557153563878	19.703813544740584
24-25	20.0786106604815	29.8581075343782	30.025384226920686	20.037897578219617
26-27	20.003512759674948	29.97242081430775	30.16817001756827	19.855896408449038
28-29	19.822920889140754	29.94689235590479	30.461656173702174	19.768530581252282
30-31	20.23185659571235	29.684616037790658	30.097202794077553	19.986324572419434
32-33	20.073497996971774	29.68816756434985	30.39995316435557	19.838381274322806
34-35	20.196466200528707	29.794036741324714	30.094903543408257	19.914593514738314
36-37	20.287669726837105	29.32677740008563	30.27235844663353	20.113194426443734
38-39	20.004862317752465	29.80028744878478	30.2036631843891	19.99118704907366
40-41	20.116320226526803	29.424977297266373	30.572135701568097	19.886566774638723
42-43	20.178752743991822	29.627067378853205	30.176312647031484	20.017867230123485
44-45	20.43741725909379	29.625780926662394	29.832895773051472	20.103906041192346
46-47	20.416157524774263	29.2912387042063	29.871302645868035	20.421301125151402
48-49	20.08899781594694	29.373346150734967	30.007870099120343	20.529785934197747
50-51	20.084232504594173	29.534736970060994	29.747508062151862	20.633522463192968
52-53	20.2936071247256	29.541753366082474	29.636318295900637	20.52832121329129
54-55	20.390004361785042	29.571517186148117	29.664786093771227	20.373692358295614
56-57	20.605418905474416	29.503679674769135	29.412458780759675	20.478442638996775
58-59	20.407708887431415	29.233801111705727	29.673279406708776	20.68521059415409
60-61	20.5897039410305	29.544563520109723	29.542373040470675	20.323359498389102
62-63	20.321583327970483	29.498939943225288	29.675914008480454	20.503562720323774
64-65	20.451721832521756	29.468863290216014	29.43735654375013	20.642058333512097
66-67	20.38701010361027	29.490806286690834	29.421893658250564	20.70028995144833
68-69	20.56854259175837	29.28666044576013	29.58045527025191	20.564341692229586
70-71	20.657431838170627	29.103563792893866	29.773070982688722	20.46593338624679
72-73	20.421779250774037	29.446518079956522	29.44414948766902	20.687553181600418
74-75	20.309204081486726	29.14682411995624	29.674975509649553	20.86899628890748
76-77	20.24313463305458	29.218790780154524	29.75932334886182	20.778751237929075
78-79	20.37927358783607	29.383780981346945	29.201339927193416	21.035605503623568
80-81	20.348776925428474	29.13045771217211	29.565635564359127	20.955129798040293
82-83	20.151752080468466	29.591521618951045	29.557780064953608	20.69894623562688
84-85	20.388228022512113	29.174575238731986	29.71744554649257	20.719751192263328
86-87	19.69363822925828	29.583315457165128	29.80989588919636	20.913150424380234
88-89	20.30048944948552	29.07652608847988	29.461578751671425	21.16140571036317
90-91	20.163870833959	29.31070389199934	29.553015352053258	20.9724099219884
92-93	20.503661264337254	29.154568592005326	29.439872364101955	20.901897779555465
94-95	20.301249186634347	28.94701324981587	29.38042094800895	21.371316615540824
96-97	20.251329986914644	29.19191675425989	29.58438802725758	20.97236523156788
98-99	19.827217896189516	29.43007200520554	29.50300677149252	21.239703327112426
100-101	20.12054852350866	29.266691471625748	29.306626859477518	21.306133145388067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	2.0
5	2.5
6	5.0
7	9.5
8	24.0
9	44.0
10	49.0
11	44.0
12	51.5
13	67.5
14	80.5
15	100.5
16	125.0
17	145.5
18	160.0
19	181.5
20	217.0
21	277.5
22	353.0
23	448.0
24	561.5
25	782.5
26	1275.0
27	2093.0
28	4001.5
29	8300.5
30	16338.5
31	28448.5
32	43265.5
33	58121.5
34	69990.5
35	77901.5
36	82723.0
37	82662.0
38	76599.5
39	67496.5
40	55790.5
41	43292.5
42	34241.5
43	28142.0
44	23812.5
45	21467.5
46	20445.0
47	20159.5
48	20366.5
49	21293.5
50	22036.0
51	20745.0
52	18860.0
53	18974.5
54	19251.5
55	18319.0
56	15964.5
57	13424.5
58	12240.0
59	11485.5
60	9597.5
61	7066.0
62	5473.5
63	3911.5
64	2489.0
65	1708.0
66	1340.5
67	1110.5
68	846.5
69	562.5
70	342.0
71	246.5
72	213.0
73	171.5
74	138.5
75	112.0
76	78.5
77	47.0
78	33.0
79	23.0
80	11.0
81	5.5
82	4.5
83	5.0
84	2.5
85	2.5
86	2.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47309279161393203
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.491179898606375E-4
22-23	0.0017429264002402555
24-25	2.681425231138855E-4
26-27	0.002636734810619874
28-29	0.003798685744113378
30-31	0.0011619509334935038
32-33	1.7876168207592365E-4
34-35	0.0036199240620374542
36-37	0.002234521025949046
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009384988308985992
46-47	0.08169408870869711
48-49	0.0014747838771263701
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008491179898606375
58-59	0.03901473711307034
60-61	0.029629748804084345
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.575233641518473E-4
78-79	0.008088966113935545
80-81	0.006256658872657328
82-83	8.491179898606375E-4
84-85	0.0029048773337337595
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.469042051898091E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06721439246054729
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1118808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.281362077571735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.47654651522738	29.375630191095837
2	13.560652556985781	11.467257215401295
3	5.453654803716814	6.917638602061171
4	3.0492483554518923	5.157054951252065
5	1.9046798532595741	4.026622925876213
6	1.3151007466800875	3.336255050331944
7	0.910991207253988	2.6962564358373045
8	0.694245566777377	2.3482918543730626
9	0.5274201663336693	2.007003871779028
>10	2.87031721239036	21.123211693581627
>50	0.15273392098238228	4.485646199085134
>100	0.08101576480254313	6.028624549579971
>500	0.0031812470045660523	0.8768989444701366
>1k	2.1208313363773682E-4	0.15360751527531893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1713	0.1531093806980286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	8.938084103796184E-5	0.0
14-15	0.0	0.0	0.0	1.7876168207592367E-4	0.0
16-17	0.0	0.0	0.0	1.7876168207592367E-4	0.0
18-19	0.0	0.0	0.0	2.234521025949046E-4	0.0
20-21	0.0	0.0	0.0	4.9159462570879E-4	0.0
22-23	0.0	0.0	0.0	7.150467283036947E-4	0.0
24-25	0.0	0.0	0.0	8.044275693416565E-4	0.0
26-27	0.0	0.0	0.0	9.384988308985991E-4	0.0
28-29	0.0	0.0	0.0	0.0012513317745314655	0.0
30-31	0.0	0.0	0.0	0.0028154964926957976	0.0
32-33	0.0	0.0	0.0	0.006882324759923061	0.0
34-35	0.0	0.0	0.0	0.013049602791542428	8.938084103796184E-5
36-37	0.0	0.0	0.0	0.022747424044161288	8.938084103796184E-5
38-39	0.0	0.0	0.0	0.040757663513310595	8.938084103796184E-5
40-41	0.0	0.0	0.0	0.06515863311667416	8.938084103796184E-5
42-43	0.0	0.0	0.0	0.09174943332546782	8.938084103796184E-5
44-45	0.0	0.0	0.0	0.12142387255007114	8.938084103796184E-5
46-47	0.0	0.0	0.0	0.1576231131704457	8.938084103796184E-5
48-49	0.0	0.0	0.0	0.20057060728918635	8.938084103796184E-5
50-51	0.0	0.0	0.0	0.25183051962445746	8.938084103796184E-5
52-53	0.0	0.0	0.0	0.3045652158368549	8.938084103796184E-5
54-55	0.0	0.0	0.0	0.3602494798035052	8.938084103796184E-5
56-57	0.0	0.0	0.0	0.4297877741310394	8.938084103796184E-5
58-59	0.0	0.0	0.0	0.5094261034958635	8.938084103796184E-5
60-61	0.0	0.0	0.0	0.6014436793444451	8.938084103796184E-5
62-63	0.0	0.0	0.0	0.6990475577578994	8.938084103796184E-5
64-65	0.0	0.0	0.0	0.7994669326640496	8.938084103796184E-5
66-67	0.0	0.0	0.0	0.911058912699945	8.938084103796184E-5
68-69	0.0	0.0	0.0	1.0448620317337738	8.938084103796184E-5
70-71	0.0	0.0	0.0	1.1975245082266126	8.938084103796184E-5
72-73	0.0	0.0	0.0	1.3706551973171446	8.938084103796184E-5
74-75	0.0	0.0	0.0	1.5496850219161824	8.938084103796184E-5
76-77	0.0	0.0	0.0	1.7274635147406883	1.3407126155694276E-4
78-79	0.0	0.0	0.0	1.9326819257638488	1.7876168207592367E-4
80-81	0.0	0.0	0.0	2.1720885084840296	1.7876168207592367E-4
82-83	0.0	0.0	0.0	2.4438509556599524	1.7876168207592367E-4
84-85	0.0	0.0	0.0	2.7158815453589895	1.7876168207592367E-4
86-87	0.0	0.0	0.0	3.0106148686816683	2.234521025949046E-4
88-89	0.0	0.0	0.0	3.3377040564600895	2.681425231138855E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1700	0.0	41.272823	1
CCGTGCG	140	0.0	40.705223	9
GGTATCA	965	0.0	38.58007	1
TCAACGC	1880	0.0	36.37488	4
ATCAACG	1895	0.0	36.086956	3
CAACGCA	2035	0.0	33.370953	5
AACGCAG	2065	0.0	32.886147	6
CGCAGAG	2255	0.0	30.325846	8
ACGCAGA	2280	0.0	29.993324	7
TATCAAC	2405	0.0	29.026804	2
AACCGTG	225	1.0186341E-10	25.327696	7
TAGACCG	95	0.0012281266	24.994436	5
GCAGAGT	2800	0.0	24.083927	9
AGTTGCG	100	0.0016557615	23.744717	2
ACCGTGC	240	2.3101165E-10	23.744717	8
TAATACT	1240	0.0	23.744715	4
AAACCGT	245	2.9831426E-10	23.26013	6
CAGTACG	105	0.0021990272	22.614016	9
AGAGTAC	2600	0.0	20.913614	10-11
TGCGCGG	165	2.7284841E-11	20.147032	12-13
>>END_MODULE