FastQCFastQC Report
Fri 10 Feb 2017
SRR3224479_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224479_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033856
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC34490.33360545375758327No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC21570.20863640584375384No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA19140.1851321654079485No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT16540.15998359539432958No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT15490.14982744211959886No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA15450.14944054104246626No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA13230.12796753126160704No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT12590.12177711402748545No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG10760.10407638974866905No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG405.3794094E-447.515249
GGTATCA7800.045.720931
GTATCAA14650.038.9486161
ATCAACG17200.031.4698163
TCAACGC17550.030.8422154
CCTCGTC3200.029.6754848
CTCGTCC3300.028.797129
ACATGGG40600.028.6521933
GTACATG43500.028.639171
CATGGGA21550.027.5410524
CAACGCA20300.026.6640825
AACGCAG21200.025.5321166
CGAGCGC950.001224178925.0080229
TCGCACC4200.024.907021
ACGCAGA22500.024.2679527
ACTTAAT15900.023.924411
CGCAGAG23100.023.8431598
TAATACT16950.023.810424
CATGGGG17650.023.6731344
TTAATAC18200.023.4795043
Produced by FastQC (version 0.11.5)