##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224479_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033856
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45329040021048	34.0	31.0	34.0	31.0	34.0
2	32.60677502476167	34.0	31.0	34.0	31.0	34.0
3	32.74249025009286	34.0	33.0	34.0	31.0	34.0
4	36.02879511266559	37.0	37.0	37.0	35.0	37.0
5	36.057790446638606	37.0	37.0	37.0	35.0	37.0
6	36.156976406772316	37.0	37.0	37.0	35.0	37.0
7	36.11398009007057	37.0	37.0	37.0	35.0	37.0
8	36.14781845827659	37.0	37.0	37.0	35.0	37.0
9	37.91252553547109	39.0	39.0	39.0	37.0	39.0
10-11	37.989361187631545	39.0	39.0	39.0	37.0	39.0
12-13	38.013139644205765	39.0	39.0	39.0	37.0	39.0
14-15	39.56801769298626	41.0	40.0	41.0	38.0	41.0
16-17	39.41437830800421	41.0	40.0	41.0	37.5	41.0
18-19	39.528566841030084	41.0	40.0	41.0	37.5	41.0
20-21	39.60623916676984	41.0	40.0	41.0	38.0	41.0
22-23	39.57166568651727	41.0	40.0	41.0	38.0	41.0
24-25	39.538684304197105	41.0	40.0	41.0	38.0	41.0
26-27	39.42021857976353	41.0	40.0	41.0	37.0	41.0
28-29	39.36721845208617	41.0	40.0	41.0	37.0	41.0
30-31	39.30567264686765	41.0	40.0	41.0	37.0	41.0
32-33	39.14830498638108	41.0	40.0	41.0	36.0	41.0
34-35	39.182719837192025	41.0	40.0	41.0	36.5	41.0
36-37	39.21235404156865	41.0	40.0	41.0	37.0	41.0
38-39	39.14055052154265	41.0	40.0	41.0	36.0	41.0
40-41	39.10301434629194	41.0	40.0	41.0	36.0	41.0
42-43	39.023418638572494	41.0	39.0	41.0	35.5	41.0
44-45	38.924462400953324	41.0	39.0	41.0	35.0	41.0
46-47	38.84689163674632	41.0	39.0	41.0	35.0	41.0
48-49	38.73022161693699	40.5	39.0	41.0	35.0	41.0
50-51	37.562479687693454	39.0	37.5	40.0	33.5	40.5
52-53	37.924668909403245	39.5	38.0	40.5	34.5	41.0
54-55	38.48206423331683	40.0	38.0	41.0	35.0	41.0
56-57	38.43775680558995	40.0	38.0	41.0	35.0	41.0
58-59	38.38335174337625	40.0	38.0	41.0	35.0	41.0
60-61	38.226359376934504	40.0	37.5	41.0	34.0	41.0
62-63	38.004583810511335	40.0	37.0	41.0	34.0	41.0
64-65	37.82137937972019	40.0	36.5	41.0	34.0	41.0
66-67	37.61633438312492	39.5	36.0	41.0	34.0	41.0
68-69	37.36189324238579	39.0	36.0	41.0	33.0	41.0
70-71	37.074314991642936	39.0	35.0	41.0	33.0	41.0
72-73	36.75476758852297	38.5	35.0	40.5	33.0	41.0
74-75	36.42551912452024	37.0	35.0	40.0	33.0	41.0
76-77	36.018855140367705	37.0	35.0	39.0	32.0	41.0
78-79	35.66922956388511	36.5	35.0	39.0	32.0	40.5
80-81	35.24393871100037	36.0	35.0	38.5	32.0	39.0
82-83	34.87442883728488	35.5	35.0	37.0	31.5	39.0
84-85	34.52315602946639	35.0	35.0	37.0	31.0	39.0
86-87	34.1898214064628	35.0	34.5	36.5	31.0	37.0
88-89	33.94764357898973	35.0	34.0	36.0	31.0	37.0
90-91	33.71601460938467	35.0	34.0	36.0	31.0	37.0
92-93	33.52272705289711	35.0	34.0	35.5	30.0	36.0
94-95	33.346183124148816	35.0	34.0	35.0	30.0	36.0
96-97	33.186645915872234	35.0	34.0	35.0	29.5	36.0
98-99	33.10356519670051	35.0	34.0	35.0	29.0	36.0
100-101	32.016738791475795	34.5	32.5	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	258.0
8	1112.0
9	875.0
10	589.0
11	716.0
12	992.0
13	1208.0
14	1231.0
15	1368.0
16	1428.0
17	1506.0
18	1666.0
19	1863.0
20	2149.0
21	2610.0
22	2987.0
23	3087.0
24	3236.0
25	3418.0
26	3632.0
27	4101.0
28	4511.0
29	5309.0
30	6439.0
31	8232.0
32	10831.0
33	15258.0
34	24206.0
35	41084.0
36	82876.0
37	185565.0
38	409782.0
39	199685.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10416263010641	22.724736299591562	14.138682001720543	23.03241906858148
2	13.531478271635509	22.462606010895133	42.00468924105485	22.00122647641451
3	16.60938913097098	30.737289078255653	30.464233378955463	22.189088411817906
4	10.921637055837563	18.793526371177418	43.160846384796336	27.123990188188685
5	11.386692150550948	40.765735266806985	36.47567939829145	11.371893184350625
6	25.957870341711033	41.62746069085056	20.753663953200448	11.66100501423796
7	24.037196669555527	33.06920886467748	26.92821824315959	15.965376222607402
8	23.34628807106599	37.83679738764393	23.045569208864677	15.771345332425405
9	25.647092051504274	16.770033737773925	22.259289494861953	35.32358471585985
10-11	22.360125588089637	28.196721787173455	31.14161933576823	18.301533288968677
12-13	23.505110963228923	25.840010601089514	30.668052417357927	19.986826018323637
14-15	21.22055697309766	27.382075494518997	27.746814400679646	23.650553131703692
16-17	20.610415845916023	30.440257903647183	28.581917125077073	20.36740912535972
18-19	20.48709340351013	29.649228893747942	29.454372182124267	20.40930552061766
20-21	21.55579950969411	28.8077690308016	29.166039713131862	20.470391746372417
22-23	21.431011887593428	29.032073853682522	28.622802653560182	20.914111605163875
24-25	20.99494709591037	29.5249031841132	28.42884963474556	21.051300085230874
26-27	20.70897687879163	29.745728612108458	28.952436316082704	20.59285819301721
28-29	20.680297836449178	29.06831318868392	29.48969682431596	20.76169215055095
30-31	21.16165113903677	28.961625216664604	28.96945996347654	20.907263680822087
32-33	21.069326869506007	28.758937414881764	29.288943527918782	20.88279218769345
34-35	20.999249411910363	29.17364700693327	29.12102846044323	20.706075120713134
36-37	21.183995774104197	28.791285444362423	28.795155312312854	21.229563469220523
38-39	20.88138606611411	29.227653030774224	28.96667500158396	20.92428590152771
40-41	21.05374652857379	28.697171688445604	29.70670257313573	20.54237920984487
42-43	21.03555644545816	28.78675321936776	29.143681546418577	21.0340087887555
44-45	20.93070049214622	28.895536826321962	28.85219893831848	21.32156374321334
46-47	21.452165485328713	28.50396960505138	28.79728898415253	21.246575925467376
48-49	20.74611938219636	28.7067541321035	28.979809567289834	21.567316918410302
50-51	20.58091262226074	29.127170515042717	28.86775334282531	21.42416351987124
52-53	20.776442754116626	28.673238826296892	29.06536306797078	21.484955351615696
54-55	21.000168301968554	28.757438173207873	28.934880679707813	21.30751284511576
56-57	21.281880648755727	28.880907979447816	28.517220966943174	21.319990404853286
58-59	20.820206822061344	28.44807028796337	29.16109802692392	21.570624863051368
60-61	21.245803786508265	28.710178296748477	29.104114465932067	20.939903450811187
62-63	20.767534979979306	28.59464247444864	29.476888275472383	21.16093427009967
64-65	21.34890992412509	28.49695479391936	28.818773585407786	21.335361696547768
66-67	21.08941432213806	28.874524689079784	28.429458079040078	21.60660290974208
68-69	21.1336491735793	28.457493113160826	29.20600160950848	21.202856103751394
70-71	21.19115234616813	28.29426922124551	29.15435031571128	21.360228116875078
72-73	20.957512458214683	28.865867200074284	28.876410254426148	21.30021008728488
74-75	21.034602497833355	28.374019205769468	28.7182644391482	21.87311385724898
76-77	20.818373158350873	28.566309041104372	29.11077558189922	21.504542218645536
78-79	21.178233573528225	27.97785956797812	28.875966273715758	21.9679405847779
80-81	20.622738310349064	28.8584098803476	28.62288929128966	21.895962518013672
82-83	20.868401825158347	29.061001514513684	28.443921864642153	21.626674795685812
84-85	21.423875356360696	28.116175643190843	28.64740887736645	21.812540123082012
86-87	20.44653667440607	29.043272576291496	29.312474050100608	21.197716699201834
88-89	20.8743707118177	28.260663429152526	28.987749501939508	21.877216357090262
90-91	21.06739236412034	28.336393076018325	28.690165748421443	21.90604881143989
92-93	21.317647809893316	28.341939768015596	28.790905956020367	21.549506466070724
94-95	21.283573340968182	27.68064411291321	28.584590116998886	22.451192429119722
96-97	21.388471895505756	28.016280797325738	29.061880958276586	21.533366348891917
98-99	21.028024953414263	27.938722044326695	28.94433554691146	22.088917455347577
100-101	21.269701336115173	27.975969861685417	28.809699149093888	21.944629653105522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	2.5
3	4.0
4	4.5
5	4.5
6	7.0
7	14.5
8	19.0
9	19.5
10	23.5
11	27.0
12	30.0
13	36.5
14	39.0
15	43.5
16	51.0
17	65.0
18	80.5
19	81.5
20	96.5
21	125.0
22	139.0
23	164.5
24	205.5
25	265.0
26	332.5
27	425.5
28	653.0
29	1114.0
30	2280.0
31	5181.5
32	11148.5
33	21098.5
34	33726.5
35	48792.0
36	68738.0
37	84825.5
38	87210.0
39	81867.0
40	71262.0
41	59137.0
42	49608.0
43	41111.5
44	34511.5
45	29785.5
46	26193.5
47	22405.5
48	20332.5
49	20494.5
50	20896.5
51	20023.0
52	19066.0
53	19060.5
54	19455.0
55	19398.5
56	16581.0
57	13678.5
58	12645.5
59	11801.5
60	9875.5
61	7567.0
62	6134.0
63	4385.0
64	2673.0
65	1726.0
66	1289.0
67	1096.0
68	853.0
69	565.0
70	364.0
71	239.0
72	170.5
73	131.0
74	107.5
75	68.5
76	44.0
77	36.5
78	27.0
79	21.0
80	16.5
81	10.0
82	7.5
83	7.5
84	7.0
85	6.0
86	5.0
87	2.5
88	1.0
89	2.5
90	3.0
91	3.5
92	3.5
93	2.5
94	2.5
95	3.5
96	2.5
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15708183731583508
2	0.0
3	9.672526928314969E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03646542651974743
16-17	0.07322102884734431
18-19	0.02669617432214931
20-21	0.040576250464281295
22-23	0.0655797325739755
24-25	0.01866797697164789
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022150086665841277
38-39	0.005078076637365358
40-41	0.005706790887705831
42-43	0.0033853844249102393
44-45	0.011461944410053238
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016008032066361273
60-61	0.017990900086665842
62-63	0.029065943419586477
64-65	0.04937824996904791
66-67	0.018426163798440018
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017555636374891666
80-81	0.059292590070570764
82-83	0.05039386529652099
84-85	0.030710272997400027
86-87	0.059486040609137064
88-89	0.03356366844125294
90-91	0.0
92-93	0.003530472328834964
94-95	0.0
96-97	0.0
98-99	9.18890058189922E-4
100-101	0.0471052061408939
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1033856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.24917416421666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.65754640652813	31.26538076958684
2	14.709329414245953	13.017513581796084
3	5.733718171098318	7.611368818843898
4	2.9071730911789415	5.145600337484043
5	1.623721189402024	3.5924160851989595
6	1.018290486621182	2.7035107855359364
7	0.679475141610025	2.104634971695044
8	0.472576743239157	1.6728904494038206
9	0.3498557198339037	1.3932744011350013
>10	1.5704044636378633	12.342334918718132
>50	0.14028848613157108	4.404530706488459
>100	0.12853461867163668	10.904113381183016
>500	0.0070915651131844525	2.2807078241625143
>1k	0.0019945026880831274	1.5617229687682603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	3449	0.33360545375758327	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	2157	0.20863640584375384	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	1914	0.1851321654079485	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1654	0.15998359539432958	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1549	0.14982744211959886	No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA	1545	0.14944054104246626	No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA	1323	0.12796753126160704	No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT	1259	0.12177711402748545	No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG	1076	0.10407638974866905	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.672526928314969E-5	0.0
9	0.0	0.0	0.0	1.9345053856629937E-4	0.0
10-11	0.0	0.0	0.0	2.4181317320787421E-4	0.0
12-13	0.0	0.0	0.0	2.9017580784944906E-4	0.0
14-15	0.0	0.0	0.0	5.319889810573232E-4	0.0
16-17	0.0	0.0	0.0	6.770768849820478E-4	0.0
18-19	0.0	0.0	0.0	8.221647889067724E-4	0.0
20-21	0.0	0.0	0.0	0.0010639779621146464	0.0
22-23	0.0	0.0	0.0	0.0013057911353225207	0.0
24-25	0.0	0.0	0.0	0.0016443295778135448	0.0
26-27	0.0	0.0	0.0	0.0022730438281540173	0.0
28-29	0.0	0.0	0.0	0.0038690107713259874	0.0
30-31	0.0	0.0	0.0	0.008753636870125047	0.0
32-33	0.0	0.0	0.0	0.024810031571127895	0.0
34-35	0.0	0.0	0.0	0.04705684350625232	0.0
36-37	0.0	0.0	0.0	0.08163612727497833	0.0
38-39	0.0	0.0	0.0	0.15272920019809336	0.0
40-41	0.0	0.0	0.0	0.2466494366720317	0.0
42-43	0.0	0.0	0.0	0.33573340968181253	0.0
44-45	0.0	0.0	0.0	0.4382138324873096	0.0
46-47	0.0	0.0	0.0	0.5532201776649746	0.0
48-49	0.0	0.0	0.0	0.682735313235112	0.0
50-51	0.0	0.0	0.0	0.816167822211217	0.0
52-53	0.0	0.0	0.0	0.9558874736907268	0.0
54-55	0.0	0.0	0.0	1.1074559706574223	0.0
56-57	0.0	0.0	0.0	1.2764833787297265	0.0
58-59	0.0	0.0	0.0	1.4836205428995914	0.0
60-61	0.0	0.0	0.0	1.7063304754240436	0.0
62-63	0.0	0.0	0.0	1.9309749133341587	0.0
64-65	0.0	0.0	0.0	2.153636483223969	0.0
66-67	0.0	0.0	0.0	2.402558963724155	0.0
68-69	0.0	0.0	0.0	2.6952012659403244	0.0
70-71	0.0	0.0	0.0	3.0301125108332303	0.0
72-73	0.0	0.0	0.0	3.381080150427139	0.0
74-75	0.0	0.0	0.0	3.723729416862697	0.0
76-77	0.0	0.0	0.0	4.070973133589204	0.0
78-79	0.0	0.0	0.0	4.479540671041228	0.0
80-81	0.0	0.0	0.0	4.937824996904792	0.0
82-83	0.0	0.0	0.0	5.4338805404234245	0.0
84-85	0.0	0.0	0.0	5.917651974743098	0.0
86-87	0.0	0.0	0.0	6.4180117927448315	0.0
88-89	0.0	0.0	0.0	6.978486365606042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	40	5.3794094E-4	47.51524	9
GGTATCA	780	0.0	45.72093	1
GTATCAA	1465	0.0	38.948616	1
ATCAACG	1720	0.0	31.469816	3
TCAACGC	1755	0.0	30.842215	4
CCTCGTC	320	0.0	29.675484	8
CTCGTCC	330	0.0	28.79712	9
ACATGGG	4060	0.0	28.652193	3
GTACATG	4350	0.0	28.63917	1
CATGGGA	2155	0.0	27.541052	4
CAACGCA	2030	0.0	26.664082	5
AACGCAG	2120	0.0	25.532116	6
CGAGCGC	95	0.0012241789	25.008022	9
TCGCACC	420	0.0	24.90702	1
ACGCAGA	2250	0.0	24.267952	7
ACTTAAT	1590	0.0	23.92441	1
CGCAGAG	2310	0.0	23.843159	8
TAATACT	1695	0.0	23.81042	4
CATGGGG	1765	0.0	23.673134	4
TTAATAC	1820	0.0	23.479504	3
>>END_MODULE