FastQCFastQC Report
Fri 10 Feb 2017
SRR3224479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033856
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC38520.3725857372786926No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC20740.20060820849325242No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA20380.19712609879905907No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT16450.15911306797078123No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16170.15640476043085302No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT15720.1520521233131113No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA15050.14557153027114028No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT12400.1199393339111056No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA12070.11674740002476168No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC11310.10939627955924229No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG11280.10910610375139285No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA10880.10523709298006685No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC10660.10310913705583757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG558.2031147E-751.806139
GGTATCA7900.046.5161671
ACCGTGC1051.029548E-940.7048158
GTATCAA16000.040.267441
TCAACGC17500.035.8202364
ATCAACG17600.035.6167143
TGCGCGG452.4177309E-531.65930212-13
TAACGCA753.0824394E-431.65934
CAACGCA19850.031.5795545
AACGCAG20900.030.447466
TATCAAC21750.029.257562
TCCGGGA650.005850451529.2239692
TCGCACC3800.028.8858071
ACGCAGA22950.027.9346777
GTACATG45700.027.882731
CGCACCA3950.027.6517942
ACATGGG44850.027.423943
CGCAGAG23350.027.252768
AAATGCG908.959098E-426.3827516
CATGGGA24000.025.129574
Produced by FastQC (version 0.11.5)