##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033856
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.195563018447444	34.0	33.0	34.0	31.0	34.0
2	33.32941241333416	34.0	34.0	34.0	31.0	34.0
3	33.45234442707689	34.0	34.0	34.0	31.0	34.0
4	36.65462211371796	37.0	37.0	37.0	35.0	37.0
5	36.694128582703975	37.0	37.0	37.0	35.0	37.0
6	36.735907128265445	37.0	37.0	37.0	37.0	37.0
7	36.71310704779002	37.0	37.0	37.0	36.0	37.0
8	36.70766528414015	37.0	37.0	37.0	36.0	37.0
9	38.565821545747184	39.0	39.0	39.0	38.0	39.0
10-11	38.59523908552062	39.0	39.0	39.0	38.0	39.0
12-13	38.60410879271388	39.0	39.0	39.0	38.0	39.0
14-15	40.240976983254924	41.0	40.0	41.0	39.0	41.0
16-17	40.25727374025009	41.0	40.0	41.0	39.0	41.0
18-19	40.27224294292435	41.0	40.0	41.0	39.0	41.0
20-21	40.28543143339111	41.0	40.0	41.0	39.0	41.0
22-23	40.22342956852792	41.0	40.0	41.0	39.0	41.0
24-25	40.20782246270274	41.0	40.0	41.0	39.0	41.0
26-27	40.17488750851183	41.0	40.0	41.0	39.0	41.0
28-29	40.1488553531633	41.0	40.0	41.0	38.5	41.0
30-31	40.10153976956172	41.0	40.0	41.0	38.0	41.0
32-33	40.07601010198712	41.0	40.0	41.0	38.0	41.0
34-35	40.02223859028724	41.0	40.0	41.0	38.0	41.0
36-37	39.96574619676241	41.0	40.0	41.0	38.0	41.0
38-39	39.909557036956784	41.0	40.0	41.0	38.0	41.0
40-41	39.835276866410794	41.0	40.0	41.0	38.0	41.0
42-43	39.800182520583135	41.0	40.0	41.0	38.0	41.0
44-45	39.65834313482729	41.0	40.0	41.0	37.0	41.0
46-47	39.52466881267797	41.0	40.0	41.0	37.0	41.0
48-49	39.508961112572734	41.0	40.0	41.0	37.0	41.0
50-51	39.45379869150055	41.0	39.5	41.0	36.0	41.0
52-53	39.33174639408196	41.0	39.0	41.0	36.0	41.0
54-55	39.22947102884734	41.0	39.0	41.0	35.0	41.0
56-57	39.10601282963972	41.0	39.0	41.0	35.0	41.0
58-59	38.94259790531757	40.5	38.5	41.0	35.0	41.0
60-61	38.78286144298626	40.0	38.0	41.0	35.0	41.0
62-63	38.61474276881887	40.0	37.5	41.0	35.0	41.0
64-65	38.41118202148075	40.0	37.0	41.0	35.0	41.0
66-67	38.16467477095456	40.0	36.5	41.0	35.0	41.0
68-69	37.918890058189916	39.0	36.0	41.0	34.5	41.0
70-71	37.60624787204408	39.0	36.0	41.0	34.0	41.0
72-73	37.29176984028724	38.5	35.0	40.5	34.0	41.0
74-75	36.93546731846601	37.5	35.0	40.0	34.0	41.0
76-77	34.91276831589699	35.5	33.0	38.0	30.5	39.0
78-79	36.13435478441872	36.5	35.0	39.0	33.5	40.0
80-81	35.92567340132475	36.0	35.0	38.5	34.0	39.0
82-83	35.59202925745946	36.0	35.0	37.0	34.0	39.0
84-85	35.24983556704222	35.0	35.0	37.0	34.0	39.0
86-87	34.94857359245388	35.0	35.0	36.5	33.0	37.0
88-89	34.70435631267797	35.0	35.0	36.0	33.0	37.0
90-91	34.49807274900954	35.0	35.0	36.0	33.0	37.0
92-93	34.350374230066855	35.0	35.0	36.0	33.0	36.0
94-95	34.201521295035285	35.0	35.0	35.0	33.0	36.0
96-97	34.11808752863068	35.0	35.0	35.0	33.0	36.0
98-99	34.04385620434567	35.0	35.0	35.0	33.0	36.0
100-101	33.30899370898848	34.5	34.0	35.0	31.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	9.0
15	33.0
16	86.0
17	153.0
18	278.0
19	431.0
20	604.0
21	837.0
22	1136.0
23	1294.0
24	1409.0
25	1785.0
26	2440.0
27	3286.0
28	4533.0
29	4857.0
30	4942.0
31	5848.0
32	7218.0
33	9823.0
34	15534.0
35	28811.0
36	70993.0
37	178670.0
38	447383.0
39	241422.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10742122006479	22.769663516538415	14.109969703950151	23.012945559446642
2	13.300788504395197	22.55671969790764	42.29912096075276	21.84337083694441
3	16.46979850191903	30.701954624241672	30.878381515414137	21.949865358425157
4	10.846094620527422	19.004580908753248	43.16490884610623	26.9844156246131
5	11.280294354339482	40.53939813668441	36.71043162684165	11.469875882134456
6	25.734628420205524	41.71112804878049	20.811699114770335	11.742544416243655
7	23.944050235235856	32.94820555280426	27.052123313111302	16.055620898848584
8	23.31775411662746	37.858850749040485	22.87920174569766	15.944193388634394
9	25.454318589822954	16.7919903739012	22.227563606537082	35.526127429738764
10-11	22.301993701250463	28.19125680945896	31.211019716478887	18.295729772811686
12-13	23.463519097437167	25.963190231521605	30.58704500433329	19.986245666707937
14-15	21.22094372910734	27.488257552309026	27.656559520861705	23.634239197721925
16-17	20.400713445586234	30.604358827534977	28.56103751392844	20.433890212950352
18-19	20.299103550204283	29.67332974805002	29.57408962176551	20.45347707998019
20-21	21.370097011500256	28.968468723354967	29.184603323607515	20.47683094153726
22-23	21.260156310454228	29.16481273698058	28.530188423740615	21.044842528824574
24-25	21.02975336352148	29.603776952061207	28.3472594415338	21.019210242883517
26-27	20.765946498077057	29.786222519713068	28.90271340024298	20.545117581966895
28-29	20.722933287935184	29.155091428261898	29.38583848576536	20.73613679803756
30-31	21.239817148782315	28.964468595903064	28.968627822712474	20.827086432602147
32-33	21.169592849290833	28.760982555105624	29.32315009205832	20.746274503545227
34-35	21.032259999090748	29.159214524012423	29.121973962560176	20.68655151433665
36-37	20.95505199552348	28.895891913537795	28.840225605727777	21.308830485210947
38-39	20.764738996533367	29.18414169865049	29.076099572861207	20.975019731954934
40-41	20.787711247988113	28.79603155565185	29.845500727374024	20.57075646898601
42-43	20.908376021418842	28.88714675931658	29.182062105360902	21.022415113903676
44-45	20.83141074194132	28.824941161448344	29.007478517761843	21.336169578848498
46-47	21.369779208698066	28.44102503540463	28.77474331038849	21.414452445508818
48-49	20.708268305080402	28.53825837613067	29.1265958017905	21.62687751699843
50-51	20.646685805373284	28.91732504333292	28.976037281787793	21.459951869506007
52-53	20.695648809552598	28.7431022725624	29.20902834536758	21.35222057251742
54-55	21.02894406958029	28.749264887953448	28.98353349015724	21.238257552309026
56-57	21.341162893841936	28.947076949566853	28.39579618185591	21.315963974735304
58-59	20.709354915496313	28.472508481333325	29.194655550459338	21.62348105271103
60-61	21.122345955480213	28.806224816540816	29.08033115296401	20.99109807501496
62-63	20.57559273245017	28.94503683298254	29.327681998266687	21.151688436300606
64-65	20.995622214312245	28.865915562708928	28.811797774545006	21.326664448433824
66-67	20.916936207750403	29.14791808530395	28.484914727002604	21.450230979943047
68-69	21.061975749040485	28.69040756159465	29.073778166398416	21.17383852296645
70-71	21.04364631051133	28.564084359910858	29.00824679645908	21.38402253311873
72-73	20.7062685712517	29.072569100532377	28.67527005695184	21.545892271264083
74-75	20.81798625727374	28.64499504766621	28.53003706512319	22.00698162993686
76-77	20.58920254774606	28.701993993354968	29.02447635306692	21.684327105832054
78-79	20.861986409034845	28.07714444632536	28.707794249232183	22.353074895407605
80-81	20.317577623018888	29.06448041656248	28.44893254897371	22.16900941144492
82-83	20.637539911534468	29.08379270086831	28.38823658000346	21.89043080759376
84-85	21.026759589296166	28.31496321946926	28.58807256477097	22.070204626463603
86-87	20.024887411786555	29.103521186702984	29.23439047604309	21.637200925467376
88-89	20.584491457224217	28.397813622013125	28.724745032190167	22.292949888572487
90-91	20.81271473009781	28.545900009285624	28.46469914572242	22.176686114894146
92-93	20.93753869010771	28.57143548037638	28.523121208369446	21.967904621146463
94-95	20.608189148198587	27.90330568280302	28.371842887210597	23.116662281787793
96-97	20.84623003590442	28.218436610127522	28.74776564627956	22.187567707688498
98-99	20.270859771573605	28.00114329268293	28.94856730531138	22.77942963043209
100-101	20.480399473073412	28.268682228616836	28.69485007660955	22.556068221700198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	3.0
4	2.5
5	4.0
6	4.5
7	11.0
8	19.5
9	16.5
10	18.0
11	26.5
12	30.0
13	33.5
14	35.0
15	36.0
16	38.0
17	40.5
18	57.0
19	73.0
20	76.5
21	89.5
22	104.0
23	124.0
24	161.5
25	209.5
26	268.0
27	395.0
28	592.0
29	1061.0
30	2218.5
31	5163.0
32	11307.5
33	21055.5
34	34213.0
35	49378.5
36	68686.5
37	84733.5
38	86445.5
39	81358.5
40	71594.5
41	59428.0
42	50191.5
43	42070.5
44	35556.0
45	30702.5
46	26334.0
47	22569.5
48	20499.0
49	20304.0
50	21204.5
51	20428.5
52	18886.0
53	18680.5
54	19149.5
55	18830.5
56	16035.0
57	13481.0
58	12656.0
59	12002.0
60	9992.0
61	7298.0
62	5826.0
63	4100.5
64	2380.5
65	1493.0
66	1113.5
67	909.5
68	693.0
69	483.5
70	294.5
71	162.5
72	112.0
73	87.5
74	73.5
75	49.5
76	35.0
77	21.5
78	11.5
79	6.5
80	5.0
81	2.5
82	1.5
83	1.0
84	1.0
85	1.5
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48449687383929674
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.18890058189922E-4
22-23	0.001547604308530395
24-25	6.287142503404729E-4
26-27	0.0023214064627955925
28-29	0.003869010771325987
30-31	9.672526928314968E-4
32-33	4.836263464157484E-5
34-35	0.0035788349634765385
36-37	0.0016443295778135446
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.00918890058189922
46-47	0.0772834901572366
48-49	0.0010156153274730718
50-51	0.0
52-53	9.672526928314969E-5
54-55	0.0
56-57	0.007883109446576698
58-59	0.03965736040609137
60-61	0.030516822458833727
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	9.672526928314969E-5
78-79	0.007834746811935123
80-81	0.005948604060913706
82-83	6.770768849820478E-4
84-85	0.0020312306549461435
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06722406215178903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1033856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.89187221248621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.61314283125057	29.858380266275507
2	15.220064212572963	13.056340985430282
3	5.92394733520033	7.622675762847324
4	2.939431366757305	5.0431085824131205
5	1.7094944767072986	3.666170932144023
6	1.047999736074273	2.69704024550502
7	0.7118855763441017	2.1373873619324315
8	0.4945260892178474	1.6968919859577978
9	0.37125777362922185	1.4331546885957047
>10	1.6652138268536403	12.58558204924951
>50	0.15967593601290941	4.85618109118428
>100	0.13271635374791776	11.053266925819274
>500	0.007700266201606703	2.23802766315179
>1k	0.002944219430026092	2.0557914594939595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	3852	0.3725857372786926	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	2074	0.20060820849325242	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	2038	0.19712609879905907	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1645	0.15911306797078123	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1617	0.15640476043085302	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1572	0.1520521233131113	No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA	1505	0.14557153027114028	No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT	1240	0.1199393339111056	No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA	1207	0.11674740002476168	No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC	1131	0.10939627955924229	No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG	1128	0.10910610375139285	No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA	1088	0.10523709298006685	No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC	1066	0.10310913705583757	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.672526928314969E-5	0.0
9	0.0	0.0	0.0	1.9345053856629937E-4	0.0
10-11	0.0	0.0	0.0	1.9345053856629937E-4	0.0
12-13	0.0	0.0	0.0	1.9345053856629937E-4	0.0
14-15	0.0	0.0	0.0	4.352637117741736E-4	0.0
16-17	0.0	0.0	0.0	5.803516156988981E-4	0.0
18-19	0.0	0.0	0.0	7.254395196236226E-4	0.0
20-21	0.0	0.0	0.0	9.672526928314969E-4	0.0
22-23	0.0	0.0	0.0	0.001209065866039371	0.0
24-25	0.0	0.0	0.0	0.001547604308530395	0.0
26-27	0.0	0.0	0.0	0.0022730438281540173	0.0
28-29	0.0	0.0	0.0	0.003965736040609137	0.0
30-31	0.0	0.0	0.0	0.00918890058189922	0.0
32-33	0.0	0.0	0.0	0.025438745821468364	0.0
34-35	0.0	0.0	0.0	0.048217546737650115	0.0
36-37	0.0	0.0	0.0	0.08294191841030085	0.0
38-39	0.0	0.0	0.0	0.15413171660269903	0.0
40-41	0.0	0.0	0.0	0.2484872167884115	0.0
42-43	0.0	0.0	0.0	0.33819990404853284	0.0
44-45	0.0	0.0	0.0	0.44082541475795467	0.0
46-47	0.0	0.0	0.0	0.5566055620898849	0.0
48-49	0.0	0.0	0.0	0.6869428624489291	0.0
50-51	0.0	0.0	0.0	0.8219229757335644	0.0
52-53	0.0	0.0	0.0	0.9627066051751888	0.0
54-55	0.0	0.0	0.0	1.1145652779497337	0.0
56-57	0.0	0.0	0.0	1.2842697629070199	0.0
58-59	0.0	0.0	0.0	1.4936316082703973	0.0
60-61	0.0	0.0	0.0	1.7182760461805127	0.0
62-63	0.0	0.0	0.0	1.944806626841649	0.0
64-65	0.0	0.0	0.0	2.1683870867896493	0.0
66-67	0.0	0.0	0.0	2.4190989847715736	0.0
68-69	0.0	0.0	0.0	2.7146430450662375	0.0
70-71	0.0	0.0	0.0	3.0521658722297884	0.0
72-73	0.0	0.0	0.0	3.4056000061904173	0.0
74-75	0.0	0.0	0.0	3.751634657050885	0.0
76-77	0.0	0.0	0.0	4.10124814287483	0.0
78-79	0.0	0.0	0.0	4.5130559768478395	0.0
80-81	0.0	0.0	0.0	4.975596214559861	0.0
82-83	0.0	0.0	0.0	5.474940417234121	0.0
84-85	0.0	0.0	0.0	5.961516884363006	0.0
86-87	0.0	0.0	0.0	6.465165361520366	0.0
88-89	0.0	0.0	0.0	7.029218769345054	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	55	8.2031147E-7	51.80613	9
GGTATCA	790	0.0	46.516167	1
ACCGTGC	105	1.029548E-9	40.704815	8
GTATCAA	1600	0.0	40.26744	1
TCAACGC	1750	0.0	35.820236	4
ATCAACG	1760	0.0	35.616714	3
TGCGCGG	45	2.4177309E-5	31.659302	12-13
TAACGCA	75	3.0824394E-4	31.6593	4
CAACGCA	1985	0.0	31.579554	5
AACGCAG	2090	0.0	30.44746	6
TATCAAC	2175	0.0	29.25756	2
TCCGGGA	65	0.0058504515	29.223969	2
TCGCACC	380	0.0	28.885807	1
ACGCAGA	2295	0.0	27.934677	7
GTACATG	4570	0.0	27.88273	1
CGCACCA	395	0.0	27.651794	2
ACATGGG	4485	0.0	27.42394	3
CGCAGAG	2335	0.0	27.25276	8
AAATGCG	90	8.959098E-4	26.382751	6
CATGGGA	2400	0.0	25.12957	4
>>END_MODULE