FastQCFastQC Report
Fri 10 Feb 2017
SRR3224478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135184
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA2520.1864125932062966No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC2450.1812344656172328No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG2390.1767960705408924No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA1990.14720677003195642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.12057639957391408No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA1610.11909693454846729No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT1470.10874067937033968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGA200.002055420871.3779141
GGTATCA1400.061.1810721
CCGTGCG352.774845E-454.302649
ACCGTGC554.9525042E-543.1633228
TAGGGCA459.6151617E-442.2041364
AACCGTG756.8973422E-637.983727
GTATCAA2500.034.185352
CCATAAT1404.5110937E-1033.9140363
GCCCATA1201.4658872E-731.723521
CAATAAG753.032095E-431.7235181
AAACCGT600.003947003731.6531016
TAATACT804.484157E-429.6747844
CATAATA1601.882654E-929.6747844
ATCTAAT650.005775190429.283251
GTTACTC650.005838291329.218253
TACAGGC650.005838291329.218257
AGTACCA650.005838291329.218255
ACGCAGA2950.028.9706368
GTACATG4150.028.6658331
TCAACGC3000.028.487795
Produced by FastQC (version 0.11.5)