##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224478_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135184
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21583915256243	34.0	31.0	34.0	31.0	34.0
2	32.390785891821515	34.0	31.0	34.0	31.0	34.0
3	32.53300686471772	34.0	31.0	34.0	31.0	34.0
4	35.81997869570363	37.0	37.0	37.0	35.0	37.0
5	35.84424192212096	37.0	37.0	37.0	35.0	37.0
6	35.95543851343354	37.0	37.0	37.0	35.0	37.0
7	35.902140785891824	37.0	37.0	37.0	35.0	37.0
8	35.94739762102024	37.0	37.0	37.0	35.0	37.0
9	37.67266096579477	39.0	39.0	39.0	35.0	39.0
10-11	37.74482187241094	39.0	39.0	39.0	36.0	39.0
12-13	37.757922535211264	39.0	39.0	39.0	35.0	39.0
14-15	39.26148434726003	41.0	40.0	41.0	37.0	41.0
16-17	39.10714655580542	41.0	40.0	41.0	36.5	41.0
18-19	39.203026985442065	41.0	40.0	41.0	36.5	41.0
20-21	39.27955601254587	41.0	40.0	41.0	37.0	41.0
22-23	39.23733577938218	41.0	40.0	41.0	37.0	41.0
24-25	39.17827553556634	41.0	40.0	41.0	36.0	41.0
26-27	39.033247277784355	41.0	39.5	41.0	36.0	41.0
28-29	38.949505858681505	41.0	39.0	41.0	35.5	41.0
30-31	38.85644380991833	41.0	39.0	41.0	35.0	41.0
32-33	38.64333057166529	41.0	39.0	41.0	35.0	41.0
34-35	38.66024455556871	41.0	39.0	41.0	35.0	41.0
36-37	38.668385311871226	41.0	39.0	41.0	35.0	41.0
38-39	38.55093428216357	40.5	39.0	41.0	35.0	41.0
40-41	38.464995857497925	40.0	39.0	41.0	35.0	41.0
42-43	38.327575748609306	40.0	38.0	41.0	34.0	41.0
44-45	38.14949624215883	40.0	38.0	41.0	33.5	41.0
46-47	37.95982142857143	40.0	38.0	41.0	33.0	41.0
48-49	37.761817226890756	40.0	37.5	41.0	33.0	41.0
50-51	36.535484968635345	39.0	36.0	40.0	31.5	40.5
52-53	36.88138759024737	39.5	36.5	40.0	31.5	40.5
54-55	37.370957361817965	40.0	36.5	41.0	33.0	41.0
56-57	37.272180879394014	40.0	36.0	41.0	32.0	41.0
58-59	37.16155388211622	40.0	35.5	41.0	32.0	41.0
60-61	36.973695111847555	40.0	35.0	41.0	32.0	41.0
62-63	36.72690924961534	39.0	35.0	41.0	31.0	41.0
64-65	36.53185658066043	39.0	35.0	41.0	31.0	41.0
66-67	36.31202657119186	38.5	35.0	41.0	31.0	41.0
68-69	36.04598547165345	38.0	35.0	41.0	30.5	41.0
70-71	35.779607793821754	37.0	35.0	40.0	30.0	41.0
72-73	35.4608200674636	37.0	35.0	40.0	30.0	41.0
74-75	35.18868727068292	36.0	35.0	39.0	30.0	41.0
76-77	34.82243830630844	36.0	35.0	39.0	29.0	41.0
78-79	34.53416454610013	35.5	34.5	38.5	29.0	40.0
80-81	34.17135163924725	35.0	34.0	37.0	29.0	39.0
82-83	33.85271185939165	35.0	34.0	37.0	29.0	39.0
84-85	33.554392531660554	35.0	34.0	36.5	29.0	38.0
86-87	33.280018345366315	35.0	34.0	36.0	28.0	37.0
88-89	33.05243224050184	35.0	34.0	36.0	27.0	37.0
90-91	32.845913717599714	35.0	34.0	35.0	27.0	36.5
92-93	32.67042327494378	35.0	34.0	35.0	27.0	36.0
94-95	32.50367647058823	35.0	33.0	35.0	26.0	36.0
96-97	32.34932388448337	35.0	33.0	35.0	25.5	36.0
98-99	32.27671913835957	35.0	33.0	35.0	25.0	35.5
100-101	31.17959595810155	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	19.0
8	82.0
9	67.0
10	53.0
11	121.0
12	254.0
13	245.0
14	276.0
15	265.0
16	300.0
17	356.0
18	430.0
19	461.0
20	555.0
21	757.0
22	852.0
23	756.0
24	701.0
25	641.0
26	717.0
27	777.0
28	810.0
29	977.0
30	1207.0
31	1422.0
32	1894.0
33	2738.0
34	4328.0
35	7477.0
36	15583.0
37	27404.0
38	43207.0
39	19449.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95364827218304	21.290231758639084	13.672129690000592	23.083990279177286
2	14.597141673570837	21.03059533672624	40.951591904367376	23.42067108533554
3	18.952686708486212	27.958190318380872	29.792726949934906	23.296396023198014
4	11.535388803408688	18.067966623269026	41.703160137294354	28.693484436027934
5	12.045804237187834	39.303467866019645	36.35933246538052	12.291395431412
6	26.65182270091135	40.54030062729317	19.791543377914547	13.016333293880933
7	23.90519588116937	33.175523730619005	24.981506687181916	17.93777370102971
8	23.820126642206176	36.617499112320985	22.07509764469168	17.487276600781158
9	24.85797135755711	16.8437093147118	22.35841519706474	35.93990413066635
10-11	23.897058823529413	26.883728843650132	29.51384779263818	19.70536454018227
12-13	24.467762457095514	24.35606284767428	29.769055509527753	21.40711918570245
14-15	21.515835429924522	26.401139558975878	27.056015983424597	25.027009027675
16-17	21.2481910408871	29.68543542709941	27.9510553957873	21.11531813622619
18-19	21.204341489038995	28.549655595918942	29.23661411205895	21.00938880298311
20-21	21.93449804278494	27.33922347770107	29.344906431060892	21.3813720484531
22-23	21.767627422243933	28.10839217776199	28.803422599221328	21.32055780077275
24-25	21.420697512688104	27.64859506088809	29.229983871683707	21.700723554740097
26-27	21.39380400047343	28.392043437093147	29.114022369511183	21.10013019292224
28-29	21.21552846490709	28.456030299443718	28.97791158717008	21.35052964847911
30-31	21.228103917623386	27.4943780329033	29.666602556515564	21.610915492957748
32-33	21.064992898567876	28.023656645756894	29.426929222393184	21.484421233282045
34-35	21.62903893951947	27.846860575216002	28.96718546573559	21.556915019528937
36-37	21.250402287582908	27.620418083150533	29.605406745114287	21.523772884152272
38-39	21.43594258005097	27.66802658687153	29.050262798723182	21.84576803435432
40-41	21.716310535251278	27.646203751308445	29.104634948346458	21.53285076509382
42-43	21.530603186814812	27.60056812297495	29.158467843351925	21.710360846858308
44-45	21.767708525984837	27.84797484742001	28.824116885518773	21.56019974107638
46-47	21.706710853355425	27.62050242632264	28.58215469286306	22.09063202745887
48-49	21.57023020475796	27.950792993253636	28.4253314001657	22.0536454018227
50-51	21.541750503018108	28.484879867439933	27.913436501361105	22.05993312818085
52-53	21.917164753225236	28.161246893123447	27.85092910403598	22.07065924961534
54-55	21.826177654160254	27.697434607645878	28.265919043673808	22.21046869452006
56-57	22.112084270327852	28.19712391999053	27.78731210794177	21.90347970173985
58-59	22.111497046301125	28.082148709583155	28.1838728411365	21.622481402979222
60-61	22.044048887310606	27.918590198863637	27.998490767045453	22.038870146780305
62-63	21.85773953319744	27.81725963441568	28.50980551743018	21.815195314956696
64-65	22.58146875011563	27.51529100078814	27.74618233755277	22.157057911543458
66-67	21.479275713466624	27.93053063791222	27.571716573880558	23.018477074740602
68-69	21.813232335187596	27.705571665285834	28.657607409160846	21.823588590365723
70-71	21.89053438276719	27.503624689312346	28.252233992188426	22.35360693573204
72-73	21.970055627884957	27.472555923777964	28.403139424783998	22.15424902355308
74-75	21.926041543377913	27.666735708367856	28.0428896910877	22.364333057166526
76-77	21.83172564800568	27.51583027577228	27.944875133151854	22.707568943070186
78-79	21.93681079861355	26.863363802153657	28.266828443648713	22.93299695558408
80-81	21.617940937014286	27.547553845015173	28.204796092073124	22.629709125897417
82-83	21.50493071102148	27.826971821865342	28.338359636626027	22.32973783048715
84-85	21.507818771711104	27.27450973138348	28.78499210169028	22.432679395215143
86-87	21.144117505560345	28.270242064710953	28.135165441108445	22.450474988620257
88-89	21.903835196791547	27.517518480424442	27.760224354942544	22.81842196784147
90-91	21.63828559592851	27.394514143685644	28.2071103089123	22.760089951473546
92-93	21.583124972259622	27.30430086845882	28.717210871269845	22.39536328801172
94-95	21.81397206770032	27.435939164398153	28.319179784589892	22.430908983311635
96-97	21.835794176825658	27.725914309385725	28.403879157296725	22.03441235649189
98-99	20.866082029241735	27.917978155618105	28.307448764087333	22.908491051052827
100-101	21.567647973002977	27.42588398975771	28.01052351138937	22.99594452584995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	1.5
3	3.5
4	3.0
5	1.5
6	4.5
7	7.5
8	9.5
9	11.5
10	11.5
11	14.5
12	16.0
13	14.5
14	19.5
15	25.0
16	29.5
17	41.5
18	47.5
19	46.5
20	50.0
21	57.0
22	71.5
23	100.0
24	152.5
25	210.5
26	271.0
27	364.0
28	526.5
29	750.0
30	1144.0
31	1784.0
32	2666.0
33	3946.0
34	5232.5
35	6118.5
36	6971.5
37	7625.0
38	7202.0
39	6582.5
40	5969.5
41	5075.0
42	4406.0
43	3798.5
44	3415.0
45	3373.5
46	3441.5
47	3350.5
48	3470.5
49	3773.5
50	4111.5
51	4075.0
52	3840.5
53	3988.5
54	4116.0
55	4097.5
56	3525.5
57	2839.0
58	2541.0
59	2341.0
60	2031.5
61	1525.0
62	1192.5
63	869.5
64	507.0
65	331.0
66	260.5
67	223.5
68	189.5
69	120.0
70	61.0
71	39.5
72	28.0
73	26.0
74	22.0
75	16.5
76	14.5
77	9.0
78	6.5
79	5.0
80	1.5
81	1.0
82	2.0
83	2.0
84	2.5
85	1.5
86	0.5
87	1.5
88	1.5
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15978222274825424
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03254823055982957
16-17	0.06842525742691442
18-19	0.01701384779263818
20-21	0.031808498047106164
22-23	0.060658066043318734
24-25	0.013315185229021184
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.014424783998106285
38-39	0.003328796307255296
40-41	0.003328796307255296
42-43	0.0014794650254467984
44-45	0.006657592614510592
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.010726121434489288
60-61	0.011835720203574387
62-63	0.022561841638063674
64-65	0.04105515445614866
66-67	0.012205586459936087
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.013685051485382887
80-81	0.05474020594153155
82-83	0.045493549532489054
84-85	0.021082376612616877
86-87	0.055849804710616645
88-89	0.030329033021659367
90-91	0.0
92-93	0.0014794650254467984
94-95	0.0
96-97	0.0
98-99	3.698662563616996E-4
100-101	0.04290448573795715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	135184.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.29213516392472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40341293685948	50.707924014676294
2	10.117683383011316	12.605042016806722
3	3.171869990143571	5.9274766244525985
4	1.6126542293579071	4.018227009113504
5	0.9072664442043012	2.825778198603385
6	0.5878231542946716	2.1970055627884957
7	0.402569796577563	1.7553852526926266
8	0.29688038095690483	1.4794650254467985
9	0.21969148190810958	1.2316546336844596
>10	1.2053343466850337	13.083648952538763
>50	0.05225094704841525	2.0956622085453898
>100	0.022562908952724767	2.0727305006509646
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	252	0.1864125932062966	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	245	0.1812344656172328	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	239	0.1767960705408924	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	199	0.14720677003195642	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.12057639957391408	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	161	0.11909693454846729	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	147	0.10874067937033968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	7.397325127233992E-4	0.0
26-27	0.0	0.0	0.0	0.0014794650254467984	0.0
28-29	0.0	0.0	0.0	0.0025890637945318973	0.0
30-31	0.0	0.0	0.0	0.006287726358148893	0.0
32-33	0.0	0.0	0.0	0.013315185229021188	0.0
34-35	0.0	0.0	0.0	0.02921943425257427	0.0
36-37	0.0	0.0	0.0	0.055110072197893246	0.0
38-39	0.0	0.0	0.0	0.09875429044857378	0.0
40-41	0.0	0.0	0.0	0.15090543259557343	0.0
42-43	0.0	0.0	0.0	0.2004675109480412	0.0
44-45	0.0	0.0	0.0	0.25631731565865784	0.0
46-47	0.0	0.0	0.0	0.31475618416380635	0.0
48-49	0.0	0.0	0.0	0.37393478518167833	0.0
50-51	0.0	0.0	0.0	0.44272990886495445	0.0
52-53	0.0	0.0	0.0	0.5063469049591668	0.0
54-55	0.0	0.0	0.0	0.5788406912060599	0.0
56-57	0.0	0.0	0.0	0.6720469878092081	0.0
58-59	0.0	0.0	0.0	0.7715410107705054	0.0
60-61	0.0	0.0	0.0	0.8762131613208664	0.0
62-63	0.0	0.0	0.0	0.9912415670493551	0.0
64-65	0.0	0.0	0.0	1.1136672979050775	0.0
66-67	0.0	0.0	0.0	1.257175405373417	0.0
68-69	0.0	0.0	0.0	1.4114096342762457	0.0
70-71	0.0	0.0	0.0	1.5656438631790746	0.0
72-73	0.0	0.0	0.0	1.737631672387265	0.0
74-75	0.0	0.0	0.0	1.9410581133861995	0.0
76-77	0.0	0.0	0.0	2.147443484436028	0.0
78-79	0.0	0.0	0.0	2.3841578885075156	0.0
80-81	0.0	0.0	0.0	2.6430642679607055	0.0
82-83	0.0	0.0	0.0	2.9223132915137886	0.0
84-85	0.0	0.0	0.0	3.1960143212214467	0.0
86-87	0.0	0.0	0.0	3.510400639128891	0.0
88-89	0.0	0.0	0.0	3.8676914427742926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGA	20	0.0020554208	71.377914	1
GGTATCA	140	0.0	61.181072	1
CCGTGCG	35	2.774845E-4	54.30264	9
ACCGTGC	55	4.9525042E-5	43.163322	8
TAGGGCA	45	9.6151617E-4	42.204136	4
AACCGTG	75	6.8973422E-6	37.98372	7
GTATCAA	250	0.0	34.18535	2
CCATAAT	140	4.5110937E-10	33.914036	3
GCCCATA	120	1.4658872E-7	31.72352	1
CAATAAG	75	3.032095E-4	31.723518	1
AAACCGT	60	0.0039470037	31.653101	6
TAATACT	80	4.484157E-4	29.674784	4
CATAATA	160	1.882654E-9	29.674784	4
ATCTAAT	65	0.0057751904	29.28325	1
GTTACTC	65	0.0058382913	29.21825	3
TACAGGC	65	0.0058382913	29.21825	7
AGTACCA	65	0.0058382913	29.21825	5
ACGCAGA	295	0.0	28.970636	8
GTACATG	415	0.0	28.665833	1
TCAACGC	300	0.0	28.48779	5
>>END_MODULE