FastQCFastQC Report
Fri 10 Feb 2017
SRR3224478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135184
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC2190.16200142028642442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.13980944490472244No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT1820.13463131731565867No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA1770.13093265475204166No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG1670.12353532962480766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.11983666706119067No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT1400.1035625517812759No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG1400.1035625517812759No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT1370.10134335424310568No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA1360.1006036217303823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1250.061.1211971
AACCGTG551.17543095E-860.4366537
AAACCGT654.3906766E-851.1387066
CCGTGCG707.869858E-847.4859439
ACCGTGC707.869858E-847.4859438
CCAGACC502.818595E-547.485948
GTATCAA2600.042.2412071
CAGACCG459.608894E-442.2097249
GTGTACT704.1295334E-640.9293671
GTATTAA951.8200808E-840.2113081
ATCAACG2500.039.888193
TCCAGAC608.266358E-539.5716177
TCAACGC2600.038.354034
GCCCATA500.001569583438.2007451
TTCGATC250.001598426338.07326594-95
CGCTACG250.001614509837.9957838-39
TCGCTAC250.001614509837.9957838-39
ACCGAAC250.001615978337.9887512-13
TCGAGCG250.001615978337.9887578-79
TTCGCTA250.001615978337.9887536-37