Basic Statistics
Measure | Value |
---|---|
Filename | SRR3224478_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 135184 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC | 219 | 0.16200142028642442 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 189 | 0.13980944490472244 | No Hit |
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT | 182 | 0.13463131731565867 | No Hit |
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA | 177 | 0.13093265475204166 | No Hit |
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG | 167 | 0.12353532962480766 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 162 | 0.11983666706119067 | No Hit |
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT | 140 | 0.1035625517812759 | No Hit |
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG | 140 | 0.1035625517812759 | No Hit |
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT | 137 | 0.10134335424310568 | No Hit |
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA | 136 | 0.1006036217303823 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 125 | 0.0 | 61.121197 | 1 |
AACCGTG | 55 | 1.17543095E-8 | 60.436653 | 7 |
AAACCGT | 65 | 4.3906766E-8 | 51.138706 | 6 |
CCGTGCG | 70 | 7.869858E-8 | 47.485943 | 9 |
ACCGTGC | 70 | 7.869858E-8 | 47.485943 | 8 |
CCAGACC | 50 | 2.818595E-5 | 47.48594 | 8 |
GTATCAA | 260 | 0.0 | 42.241207 | 1 |
CAGACCG | 45 | 9.608894E-4 | 42.209724 | 9 |
GTGTACT | 70 | 4.1295334E-6 | 40.929367 | 1 |
GTATTAA | 95 | 1.8200808E-8 | 40.211308 | 1 |
ATCAACG | 250 | 0.0 | 39.88819 | 3 |
TCCAGAC | 60 | 8.266358E-5 | 39.571617 | 7 |
TCAACGC | 260 | 0.0 | 38.35403 | 4 |
GCCCATA | 50 | 0.0015695834 | 38.200745 | 1 |
TTCGATC | 25 | 0.0015984263 | 38.073265 | 94-95 |
CGCTACG | 25 | 0.0016145098 | 37.99578 | 38-39 |
TCGCTAC | 25 | 0.0016145098 | 37.99578 | 38-39 |
ACCGAAC | 25 | 0.0016159783 | 37.98875 | 12-13 |
TCGAGCG | 25 | 0.0016159783 | 37.98875 | 78-79 |
TTCGCTA | 25 | 0.0016159783 | 37.98875 | 36-37 |