##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135184
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16345869333649	34.0	33.0	34.0	31.0	34.0
2	33.29282311516156	34.0	33.0	34.0	31.0	34.0
3	33.41594419457924	34.0	34.0	34.0	31.0	34.0
4	36.62487424547284	37.0	37.0	37.0	35.0	37.0
5	36.667778731210795	37.0	37.0	37.0	35.0	37.0
6	36.704336311989586	37.0	37.0	37.0	36.0	37.0
7	36.68050213042964	37.0	37.0	37.0	36.0	37.0
8	36.662275121316135	37.0	37.0	37.0	35.0	37.0
9	38.52143744821873	39.0	39.0	39.0	37.0	39.0
10-11	38.55139661498402	39.0	39.0	39.0	37.5	39.0
12-13	38.54862631672387	39.0	39.0	39.0	37.5	39.0
14-15	40.15451902592022	41.0	40.0	41.0	38.0	41.0
16-17	40.16948751331519	41.0	40.0	41.0	38.0	41.0
18-19	40.171458900461595	41.0	40.0	41.0	38.5	41.0
20-21	40.183516540418985	41.0	40.0	41.0	39.0	41.0
22-23	40.116988696887205	41.0	40.0	41.0	38.5	41.0
24-25	40.09535152089005	41.0	40.0	41.0	38.0	41.0
26-27	40.04341490117173	41.0	40.0	41.0	38.0	41.0
28-29	39.99976328559593	41.0	40.0	41.0	38.0	41.0
30-31	39.923252751804945	41.0	40.0	41.0	38.0	41.0
32-33	39.858429991715	41.0	40.0	41.0	38.0	41.0
34-35	39.77855737365368	41.0	40.0	41.0	37.5	41.0
36-37	39.67943691561132	41.0	40.0	41.0	37.0	41.0
38-39	39.573041188306306	41.0	40.0	41.0	37.0	41.0
40-41	39.442726210202395	41.0	40.0	41.0	36.5	41.0
42-43	39.362317286069356	41.0	39.5	41.0	36.0	41.0
44-45	39.126353710498286	41.0	39.0	41.0	35.0	41.0
46-47	38.88987971949343	41.0	39.0	41.0	35.0	41.0
48-49	38.78607675464552	41.0	39.0	41.0	35.0	41.0
50-51	38.665026926263465	40.5	38.5	41.0	35.0	41.0
52-53	38.47637294354361	40.0	38.0	41.0	35.0	41.0
54-55	38.323847496745174	40.0	37.5	41.0	35.0	41.0
56-57	38.140841371759976	40.0	37.0	41.0	35.0	41.0
58-59	37.94527089004616	40.0	36.5	41.0	34.0	41.0
60-61	37.73554192803882	40.0	36.0	41.0	34.0	41.0
62-63	37.574483666706115	39.5	35.0	41.0	34.0	41.0
64-65	37.34352068292105	39.0	35.0	41.0	34.0	41.0
66-67	37.09093161912652	39.0	35.0	41.0	33.0	41.0
68-69	36.87432684341342	38.5	35.0	41.0	33.0	41.0
70-71	36.608196975973485	37.5	35.0	40.5	33.0	41.0
72-73	36.3173896319091	37.0	35.0	40.0	33.0	41.0
74-75	35.98467644099893	36.5	35.0	39.0	33.0	41.0
76-77	34.05334950881761	34.5	33.0	37.5	29.5	39.0
78-79	35.338261184755595	36.0	35.0	39.0	32.5	40.0
80-81	35.213553379098116	35.5	35.0	37.5	33.0	39.0
82-83	34.93186693691561	35.0	35.0	37.0	33.0	39.0
84-85	34.640046159308795	35.0	35.0	37.0	33.0	39.0
86-87	34.37016214936679	35.0	35.0	36.0	32.0	37.0
88-89	34.14911897857735	35.0	35.0	36.0	32.0	37.0
90-91	33.96599449639011	35.0	35.0	36.0	32.0	37.0
92-93	33.82039664457332	35.0	35.0	35.0	32.0	36.0
94-95	33.703378358385606	35.0	34.5	35.0	32.0	36.0
96-97	33.62074653805184	35.0	34.0	35.0	31.0	36.0
98-99	33.55092688483845	35.0	34.0	35.0	31.0	36.0
100-101	32.7893722629897	34.5	33.0	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	6.0
16	22.0
17	38.0
18	76.0
19	175.0
20	241.0
21	387.0
22	626.0
23	574.0
24	407.0
25	343.0
26	440.0
27	532.0
28	681.0
29	797.0
30	855.0
31	1039.0
32	1248.0
33	1711.0
34	2655.0
35	5124.0
36	13847.0
37	29030.0
38	48311.0
39	25991.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22062015042959	21.681481701697535	13.694740910307102	22.403157237565775
2	14.423304533080838	21.454462066516747	42.086341578885076	22.03589182151734
3	18.437093147118002	28.348029352586106	30.499911232098476	22.71496626819742
4	11.727719256716771	17.965143804000476	42.425139069712394	27.881997869570363
5	11.542786128535921	39.012013256006625	37.30840927920464	12.136791336252811
6	27.00689430701858	40.25624334240738	20.16214936678897	12.574712983785064
7	24.07311516155758	32.9129186886022	25.40907207953604	17.604894070304177
8	23.403657237542905	36.74695230204758	22.324387501479464	17.52500295893005
9	24.877204402887916	16.831133861995504	22.41019647295538	35.88146526216121
10-11	23.943292105574624	26.649233637116822	30.022044028879158	19.3854302284294
12-13	24.6427091963546	24.730737365368682	29.917002012072437	20.709551426204285
14-15	21.808424073854894	26.90222215646822	27.119703515208897	24.169650254467985
16-17	20.825319564445497	29.754260859273284	28.103547757131018	21.316871819150197
18-19	20.79757959521837	28.532592614510595	29.882234583974437	20.787593206296602
20-21	21.843191501952894	27.515460409515917	29.769055509527753	20.87229257900343
22-23	21.699763654588022	28.052609970891417	29.211405238065296	21.036221136455264
24-25	21.46608671208658	27.79121635116842	29.12459406878084	21.61810286796417
26-27	21.444053202349426	28.49047950170881	29.3219511473421	20.743516148599667
28-29	21.259801745820386	28.366992158603345	29.343098091433646	21.030108004142626
30-31	21.44751393476275	27.59730292528304	29.371927787045017	21.583255352909195
32-33	21.28728251864126	28.121301337436382	29.695082258255418	20.896333885666944
34-35	21.86829607492122	27.71966681954698	29.091521060496223	21.320516045035582
36-37	21.290847557930945	27.704399607937418	29.51417528156381	21.490577552567824
38-39	21.610545626701384	27.962258847200854	28.80999230678187	21.617203219315893
40-41	21.58391525624334	27.853148301574148	29.25864007574861	21.3042963664339
42-43	21.586504320037875	27.95523138832998	29.033761391880695	21.42450289975145
44-45	21.79532230799676	27.821651433158127	28.670930322881333	21.71209593596378
46-47	21.72562751942432	27.76077082816647	28.616958663858362	21.896642988550845
48-49	21.544049384725206	27.562905236215958	28.894798551598385	21.998246827460452
50-51	21.545449165581726	28.146822109125342	28.220425494141317	22.087303231151616
52-53	21.865383477334596	28.274056101313764	28.169383950763404	21.691176470588236
54-55	21.75553319919517	27.555405965202983	28.601757604450228	22.087303231151616
56-57	21.949451272965344	27.663462214216228	28.040376840649078	22.34670967216935
58-59	22.112296695327878	27.57062941202587	28.56994864660875	21.747125246037502
60-61	21.90603033666297	27.803551609322973	28.416574176840548	21.87384387717351
62-63	21.822848857853	27.67820156231507	28.528154219434253	21.97079536039768
64-65	22.17976979524204	27.575748609302874	28.22967215055036	22.014809444904724
66-67	21.556915019528937	27.77584625399456	27.97964256124985	22.687596165226655
68-69	21.797328086164043	27.716667652976685	28.41053674991123	22.07546751094804
70-71	22.15498875606581	27.66599597585513	28.018478518167832	22.160536749911234
72-73	22.10986507278968	27.44592555331992	28.189726594863295	22.254482779027104
74-75	21.799547283702214	27.540981181204877	28.236329743164873	22.42314179192804
76-77	21.81775142676436	27.545521457870226	28.042623545033234	22.594103570332177
78-79	21.777292818701337	27.091046956779053	28.25322261471029	22.878437609809325
80-81	21.50540020713123	27.62538837106081	28.21608226068945	22.65312916111851
82-83	21.401808666062543	27.758770550921902	28.715255303016292	22.12416547999926
84-85	21.43472712814159	27.631165276570563	28.443030717733436	22.491076877554416
86-87	21.225144987572495	28.08394484554385	28.284412356491895	22.406497810391762
88-89	21.84947922831104	27.512131613208663	27.701133270209493	22.9372558882708
90-91	21.76810865191147	27.634557344064387	28.03771156349864	22.559622440525505
92-93	21.53768197419813	27.59978991596639	28.586962954195762	22.275565155639722
94-95	21.646052787312108	27.745147354716536	27.79286010178719	22.815939756184164
96-97	21.754793466682447	27.71851698425849	28.2766451651083	22.250044383950762
98-99	20.475056219670968	28.632826369984617	28.236699609421233	22.655417800923185
100-101	21.291896133120154	28.026736542902825	27.881284419968022	22.800082904009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	2.0
3	3.0
4	2.5
5	4.0
6	3.5
7	5.5
8	16.0
9	20.5
10	20.0
11	21.5
12	22.0
13	25.5
14	26.5
15	22.0
16	20.5
17	28.0
18	35.5
19	39.5
20	45.0
21	65.5
22	90.5
23	117.5
24	144.0
25	194.0
26	291.5
27	390.5
28	505.0
29	770.5
30	1228.0
31	1929.0
32	2896.0
33	4076.0
34	5392.0
35	6461.5
36	7222.5
37	7592.5
38	7336.5
39	6579.5
40	5664.5
41	4851.0
42	4178.5
43	3682.0
44	3414.0
45	3398.5
46	3361.5
47	3364.5
48	3524.5
49	3836.5
50	4170.5
51	4051.0
52	3721.0
53	3804.5
54	4122.5
55	4053.0
56	3374.0
57	2763.5
58	2590.0
59	2507.0
60	2037.0
61	1458.5
62	1174.5
63	807.0
64	467.0
65	297.5
66	233.0
67	190.0
68	150.0
69	106.5
70	56.5
71	33.5
72	21.0
73	16.0
74	15.0
75	13.0
76	8.0
77	7.5
78	4.5
79	0.0
80	1.0
81	1.5
82	1.0
83	1.0
84	1.0
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47046987809208185
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.698662563616996E-4
24-25	7.397325127233992E-4
26-27	0.0014794650254467984
28-29	0.002958930050893597
30-31	3.698662563616996E-4
32-33	0.0
34-35	0.0014794650254467984
36-37	0.0011095987690850988
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007767191383595692
46-47	0.08026097763048881
48-49	3.698662563616996E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0055479938454254946
58-59	0.03106876553438277
60-61	0.025150905432595575
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.698662563616996E-4
78-79	0.004808261332702095
80-81	0.002958930050893597
82-83	0.0011095987690850988
84-85	0.0011095987690850988
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06509646111965914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	135184.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.14617114451415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93309391449796	48.07669546691916
2	10.921434545186205	13.13764942596757
3	3.4154080779259113	6.162711563498639
4	1.6714222462734292	4.021185939164399
5	1.018349977861957	3.062492602674873
6	0.6346238992473066	2.290211859391644
7	0.4612092291041472	1.9417978458989231
8	0.30378314556993163	1.4617114451414368
9	0.25212771191026717	1.3648064859746716
>10	1.307374428100556	14.057136939282755
>50	0.0541152162148866	2.171114924843177
>100	0.0270576081074433	2.2524855012427505
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	219	0.16200142028642442	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.13980944490472244	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	182	0.13463131731565867	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	177	0.13093265475204166	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	167	0.12353532962480766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	162	0.11983666706119067	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	140	0.1035625517812759	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	140	0.1035625517812759	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	137	0.10134335424310568	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	136	0.1006036217303823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	7.397325127233992E-4	0.0
26-27	0.0	0.0	0.0	0.0014794650254467984	0.0
28-29	0.0	0.0	0.0	0.0025890637945318973	0.0
30-31	0.0	7.397325127233992E-4	0.0	0.006287726358148893	0.0
32-33	0.0	7.397325127233992E-4	0.0	0.013315185229021188	0.0
34-35	0.0	7.397325127233992E-4	0.0	0.02958930050893597	0.0
36-37	0.0	7.397325127233992E-4	0.0	0.055479938454254946	0.0
38-39	0.0	7.397325127233992E-4	0.0	0.0980145579358504	0.0
40-41	0.0	7.397325127233992E-4	0.0	0.15053556633921172	0.0
42-43	0.0	7.397325127233992E-4	0.0	0.20120724346076457	0.0
44-45	0.0	7.397325127233992E-4	0.0	0.2577967806841046	0.0
46-47	0.0	7.397325127233992E-4	0.0	0.31549591667652976	0.0
48-49	0.0	7.397325127233992E-4	0.0	0.3754142502071251	0.0
50-51	0.0	7.397325127233992E-4	0.0	0.4445792401467629	0.0
52-53	0.0	7.397325127233992E-4	0.0	0.5130044975736774	0.0
54-55	0.0	7.397325127233992E-4	0.0	0.5858681500769322	0.0
56-57	0.0	7.397325127233992E-4	0.0	0.6812936442182507	0.0
58-59	0.0	7.397325127233992E-4	0.0	0.7844863297431648	0.0
60-61	0.0	7.397325127233992E-4	0.0	0.891377677831696	0.0
62-63	0.0	7.397325127233992E-4	0.0	1.008255414841993	0.0
64-65	0.0	7.397325127233992E-4	0.0	1.129201680672269	0.0
66-67	0.0	7.397325127233992E-4	0.0	1.2749289856787787	0.0
68-69	0.0	7.397325127233992E-4	0.0	1.4306426796070542	0.0
70-71	0.0	7.397325127233992E-4	0.0	1.588205704817138	0.0
72-73	0.0	7.397325127233992E-4	0.0	1.7613031127944137	0.0
74-75	0.0	7.397325127233992E-4	0.0	1.9669487513315185	0.0
76-77	0.0	7.397325127233992E-4	0.0	2.1751834536631556	0.0
78-79	0.0	7.397325127233992E-4	0.0	2.4174458515800685	0.0
80-81	0.0	7.397325127233992E-4	0.0	2.678571428571429	0.0
82-83	0.0	7.397325127233992E-4	0.0	2.9633684459699374	0.0
84-85	0.0	7.397325127233992E-4	0.0	3.2378092081903187	0.0
86-87	0.0	7.397325127233992E-4	0.0	3.5577435199431884	0.0
88-89	0.0	7.397325127233992E-4	0.0	3.921691916203101	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	125	0.0	61.121197	1
AACCGTG	55	1.17543095E-8	60.436653	7
AAACCGT	65	4.3906766E-8	51.138706	6
CCGTGCG	70	7.869858E-8	47.485943	9
ACCGTGC	70	7.869858E-8	47.485943	8
CCAGACC	50	2.818595E-5	47.48594	8
GTATCAA	260	0.0	42.241207	1
CAGACCG	45	9.608894E-4	42.209724	9
GTGTACT	70	4.1295334E-6	40.929367	1
GTATTAA	95	1.8200808E-8	40.211308	1
ATCAACG	250	0.0	39.88819	3
TCCAGAC	60	8.266358E-5	39.571617	7
TCAACGC	260	0.0	38.35403	4
GCCCATA	50	0.0015695834	38.200745	1
TTCGATC	25	0.0015984263	38.073265	94-95
CGCTACG	25	0.0016145098	37.99578	38-39
TCGCTAC	25	0.0016145098	37.99578	38-39
ACCGAAC	25	0.0016159783	37.98875	12-13
TCGAGCG	25	0.0016159783	37.98875	78-79
TTCGCTA	25	0.0016159783	37.98875	36-37
>>END_MODULE